1.Air Quality Improvement by Ventilation in Outpatient Department Area
Yuhua GAO ; Chenjie TIAN ; Xiaobo LIU
Chinese Journal of Nosocomiology 2004;0(10):-
OBJECTIVE To evaluate the methods of improving the air quality in the outpatient departmant area.METHODS The amount of outpatients in different time periods in the morning were calculated in the specialist consulting area;the bacteria in the air collected from the clinic room were collected and cultured in general nutrition agar medium and compared the efficacy for the improvement of air quality between the new and the old methods.RESULTS The air quality of the specialist consulting area was improved significantly through the ventilation and cleaning-floors depending on the amount of outpatients(P
2.The changes of macular microstructure in large idiopathic macular hole after vitrectomy combined with internal limiting membrane transplantation or internal limiting membrane peeling
Ruijie XI ; Yuhua HAO ; You HAN ; Xiaoyu TIAN ; Junfang SHI ; Yuhua WEI
Chinese Journal of Ocular Fundus Diseases 2016;32(5):468-472
Objective To observe the different changes of macular microstructure in patients with large idiopathic macular hole (IMH) treated with vitrectomy combined with internal limiting membrane (ILM) transplantation or not.Methods Forty eyes in 40 consecutive patients with giant IMH (≥500 μm) were included in the study.Twenty eyes received vitrectomy with ILM transplantation (ILM transplantation group) and others with ILM peel off (ILM removal group).During the operation,a proper size of the ILM was removed and filled in the bottom of the macular hole.The age,duration of disease and the ocular laterality of the two groups of patients were not statistically significant (P>0.05).Minimum resolution angle in logarithmic (logMAR) best corrected visual acuity (BCVA) and frequency domain optical coherence tomography (SD-OCT) scan were examined.There was no statistically significant difference in logMAR BCVA,average defect diameter of photoreceptor ellipsoid (IS/OS) and average defect diameter of external limiting membrane (ELM) between two groups (t=0.128,1.452,1.321;P>0.05).The logMAR BCVA and SD-OCT were examined on 1,3,6,12 months postoperatively.Results On 1 month after the surgery,there was no statistically significant difference in logMAR BCVA,average defect diameter of IS/OS and average defect diameter of ELM between two groups (t=1.226,1.435,1.018;P>0.05).On 3,6,12 months after the surgery,compared with ILM removal group,the logMAR BCVA (t=2.059,2.871,2.415) increased and the average defect diameter of IS/OS (t =2.070,2.110,2.121) and ELM (t =2.034,3.647,3.556) significantly reduced in ILM transplantation group (P<0.05).On 1 month after the surgery,there was statistically significant difference in CRT between two groups (t=2.113,P<0.05).On 3,6,12 months after the surgery,there was no statistically significant difference in CRT between two groups (t=0.428,0.847,0.849;P>0.05).Conclusion Compared with vitrectomy combined with ILM peeling surgery,the diameter of IS/OS and ELM defect were significantly decreased after vitrectomy combined with ILM transplantation in the patients with large IMH.
3.Bibliometric analysis of neonatal pressure sore literature in China
Jian ZHANG ; Yandong WANG ; Tian HE ; Hui LIU ; Yuhua TAN
Chinese Journal of Practical Nursing 2017;33(22):1753-1756
Objective Through the analysis of the status quo of the research on neonatal pressure sore in the relevant nursing research, we can understand the strategy and the problems of the prevention of neonatal pressure sore, which provides reference for future research. Methods This article used bibliometric analysis method to construct literature reading and reviewing database,screening articles in the Chinese biological medical disc (CBMdisc), China Journal Full-text Database (CJFD), China Science Periodical Database(CSPD)and China Science and Technology Journal Database(CSTJ), according to the criteria of inclusion and exclusion. Results A total of 74 articles were integrated into the literature, the first published in China in 2006, after the overall trend of growth, especially since 2011 began to significantly increase. Of which 29 (39.2%) of the literature published in 13 kinds of professional nursing journals. The total number of citations was 1.84, the cohort rate was 39.19%, the total number of citations was 54, and the number of citations per article was 0.73. The total number of citations was 447, and the average number of citations was 6.04. Literature research types are subdivided into 10 categories, of which experienced literature was the most (23.0%). Literature research concentrated on the use of chemical or physical methods to prevent neonatal pressure sores. Conclusions Neonatal pressure sore is paid more and more attention by the nurses, and the policy support for it is acceptable, but the related research needs to be further strengthened in the breadth, depth and comprehensive aspects. It is necessary for us to use scientific research methods, combining with the culture of pediatric and the characteristics of pediatric management in our country, to construct the best practice manual for preventing neonatal pressure sore in China.
4.Use of electronic resources in readers of hospital library and measures for improving its information service
Xinyu TIAN ; Rui XU ; Yahan SONG ; Shumei YIN ; Yuhua WANG
Chinese Journal of Medical Library and Information Science 2014;(12):52-54
The use of electronic resources and needs of novel services in readers of our library and their suggestions for library website construction were investigated with questionnaires and suggestions were put forward for improving and deepening the information service in hospital library.
5.Therapeutic Effect Observations on Treatment of Urticaria with Acupuncture plus Garlic-partitioned Moxibustion
Ping GENG ; Yuhua TIAN ; Zhaojuan LIN ; Guifen WANG ; Siyou WANG
Journal of Acupuncture and Tuina Science 2004;2(1):44-45
Forty-six patients with intractable urticaria were treated by acupuncture plus garlic-partitioned moxibustion. The results were compared with those of simple acupuncture in a control group of 30patients. The total effective rate were 91.3%, in the observation group and 73.3%, in the control group. It is showed that acupuncture plus garlic-partitioned moxibustion has a better effect on intractable urticaria than simple acupuncture.
6.Observing on relationship in changes of OPN,IL-18 levels with hepatic function in patients with chronic hepatic disease infected by HBV
Liulan PAN ; Xiaoqi ZHOU ; Yuhua ZHOU ; Yueli TIAN ; Dongfu LI
Chinese Journal of Immunology 1999;0(12):-
Objective:To observe clinical significance between changes of OPN,IL-18 levels and relation ship with hepatic function in patients with chronic hepatic disease infected by HBV.Methods:The levels of OPN,IL-18 in peripheral blood of 102 patients with chronic hepatitis B,34 patients with hepatitis B-related cirrhosis,95 HBV carriers and 20 healthy people as the control group were respectively detected with ELISA.Results:The levels of OPN and IL-18 in chronic hepatitis B and hepatitis B-related cirrhosis groups were significantly increased compared with that of health control group(P
7.Methylation status of p16 gene and expressions of related genes in keloid tissue and cultured keloid fibroblasts
Jiang JI ; Hong LENG ; Shengjun JI ; Yuhua SU ; Xin SHI ; Ye TIAN ; Jianping CAO
Chinese Journal of Dermatology 2015;48(3):171-174
Objective To explore the role of p16 gene methylation in fibroblasts in the occurrence and development of keloid.Methods Skin tissue specimens were resected from the lesions of patients with keloid and normal skin of healthy human controls.Fibroblasts were isolated from these tissue specimens and subjected a primary culture.An immunohistochemical analysis was performed to measure the expression of p16 protein in tissue specimens,real-time fluorescence-based quantitative PCR to determine the mRNA expression level (expressed as 2-△△Ct) of p 16 and DNA methyltransferases (DNMTs) in fibmblasts,and bisulfite sequencing PCR (BSP) to estimate the methylation status of p16 gene in the tissue specimens and primary fibroblasts.Results The keloid fibroblasts (KFbs) showed significandy lower mRNA expression of p16 gene (0.64 ± 0.18 vs.1.92 ± 0.23,t =10.54,P< 0.05),but significantly higher mRNA expressions of 3 DNMTs (DNMT1:2.58 ± 0.23 vs.1.13 ± 0.21,t =11.22,P < 0.05; DNMT3A:4.87 ± 0.46 vs.2.38 ± 0.32,t =10.81,P< 0.05; DNMT3B:1.57 ± 0.12 vs.0.57 ± 0.16,t =12.45,P< 0.05) compared with the normal fibmblasts (NFbs).The DNA methylation rate in the p16 gene promoter region was significantly increased in keloid tissue (1.81% ± 0.46%) and KFbs (3.15% ± 0.94%) compared with normal skin tissue (0.90% ± 0.35%,F =14.23,P< 0.01) and NFbs (0.17% ± 0.29%,F=37.62,P< 0.01).Conclusions The methylation and low expression of p16 gene in KFbs may be associated with the uncontrolled growth of keloid,and DNMTs may play a role in the pathogenesis of keloid.
8.The preliminary establishment of the satisfaction evaluation tool for using personal digital assistant by nurse
Hui LIU ; Haina LIU ; Yandong WANG ; Hong ZHANG ; Yuhua TAN ; Tian HE ; Jian ZHANG ; Xueting WAN
Chinese Journal of Practical Nursing 2017;33(20):1567-1570
Objective Based on the theory of Service Quality to develop a scale for measuring personal digital assistant satisfaction by nurses and to detect the reliability and validity of this scale. Methods Through the literature research, cross-sectional study and present satisfaction evaluation tool for using personal digital assistant by nurse to build the item pool. The items were selected by 15 experts consultation and the pilot survey of 666 nurses. Results The satisfaction evaluation tool for using personal digital assistant by nurse scale consisted of 41 items;the exploratory factor analysis identified 8 principal factors and explained for 65.22%. Pearson correlation coefficient between each dimension was 0.213-0.684(P<0.01). Pearson correlation coefficient between each dimension and total scale was 0.574-0.798(P<0.01). The Cronbach α coefficient of the scale was 0.928 and test-retest reliability was 0.934. Conclusions The satisfaction evaluation tool for using personal digital assistant by nurse scale has good validity and reliability. It can be used as a tool to measure the satisfaction for using personal digital assistant by nurse.
9.Alterations of calcium channel gene expression and function in rat ventricular myocytes infected by virus
Miao TIAN ; Shujun HUANG ; Yuhua LIAO ; Jihua DONG ; Min WANG ; Heping GUO ; Ming TANG
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To investigate the changes of the L-type calcium channel subunit expression and calcium currents (I_~Ca -L) in cultured rat ventricular myocytes infected by coxsackie virus B_3 (CVB_3). METHODS: Primary cultured neonatal rat ventricular myocytes were infected with CVB_3. The changes of L-type calcium channel subunits mRNA in normal and infected myocytes were measured by semi-quantitative reverse transcription-polymerase chain reaction. I_~Ca -L was recorded in two groups respectively using whole cell patch-clamp techniques. RESULTS: The expression of ?_1 and ? subunits of L-type calcium channel mRNA increased in the infected group compared with the normal one (4.00?0.07 vs 2.21?0.41, P0.05). The average current density of I_~Ca -L significantly increased by CVB_3 infection [(-8.66?0.99) pA/pF vs (-6.97?1.75) pA/pF, P
10.Cytokine production in mice with experimental cardiomyopathy treated with anti-L3T4 monoclonal antibody at different stages
Zhaohui WANG ; Yuhua LIAO ; Jing YUAN ; Min WANG ; Jinghui ZHANG ; Yuan TIAN ; Jihua DONG
Chinese Journal of Pathophysiology 1989;0(06):-
AIM:To clarify the mechanism of treating autoimmune cardiomyopathy at different stages with anti-L3T4 monoclonal antibody.METHODS:Mice immunized with human mitochondria ADP/ATP peptides were used as the cardiomyopathy(DCM) group,and the sham-immunized mice were regarded as the controls.Mice receiving early treatment were immunized with the same peptides,followed by the injection of 400 ?g of anti-L3T4 on day 0,1 and 2 post-immunization.Mice in the late treatment group were immunized as of the early treatment group but anti-L3T4 was administered 3 months post-immunization.The cytokine expression was measured with three-color flow cytometry to quantitate the splenic Th1/Th2 cell subsets in the different groups of mice.In addition,serum and myocardial cytokines were measured by enzyme-linked immunosorbent assay and real-time PCR.RESULTS:Th1 and Th2 subsets in the early treatment group were similar to those in control group,but were drastically lower than those in DCM group.Mice in the late treatment group showed an increased level of Th1-related cytokines,while the Th2 level was between the DCM and early treatment group.IFN-? and IL-6 levels in early treatment group were similar to those in control group.In the early treatment group,IL-4 level was higher than that in control and lower than that in DCM group,whereas IL-2 and TNF-? contents were lower than those in control and DCM group.In the late treatment group,IFN-? and IL-2 levels were higher than those in DCM group and lower than those in the early treatment group,while IL-6 and IL-4 levels were lower than those in DCM group.CONCLUSION:These results suggest that the cytokine production in cardiomyopathic mice may be repressed by treatment with anti-L3T4 at different stages.Early treatment with anti-L3T4 has better inhibitory function than treatment in late stage of autoimmune cardiomyopathy.