1.Expression of VEGF gene mediated by adenovirus in human bone marrow stromal cell
Bin CHEN ; Yanbin SONG ; Yuhua LI
Orthopedic Journal of China 2006;0(20):-
[Objective]To investigate the possibility of stable expression of VEGF~65 in human bone marrow stromal cell.[Method]hBMSc were divided into transfection group, empty vector group and control group, hBMSc were infected with Ad-VEGF165(MOI=20 pfu). The hBMSc proliferation and transfection efficiency were confirmed by fluorescence microscope.The expression of VEGF165 gene of cultured hBMSc transfected with VEGF165 gene was assayed by RT-PCR, ELISA and Western blot.[Result]VEGF165 gene was amplified with RT-PCR.GAPDH gene(internal control) was located at 549 bp in the three groups, VEGF165 gene was located at 500 bp in transfection group but not in the other groups. ELISA assay showed that the expression had significant difference in the transfection group compared with the empty vector group and control group (P
2.Cellular tunneling nanotubes and its significance
Kefu WU ; Yuhua SONG ; Xiaotong MA
Journal of Leukemia & Lymphoma 2009;18(4):195-196
Tunneling nanotubes(TNT) are cellular communication and transport mechanism discovered recently. TNTs play important role in immunological response and viral spread, also may involve embryonic development, regulation of gene expression and carcinogenesis. However, it was not easy to study TNT for clinical samples since intricate assay method for TNT. We suggested using histochemical assay PAS staining in cell drip to show TNT in leukemia and lymphoma samples.
3.Qualitative Study on Training Demand among Clinical Medical Postgraduate Students
Yuhua MA ; Hong SONG ; Shanhua TAI ; Jinhua HUANG
Chinese Journal of Medical Science Research Management 2013;26(5):336-338
Objective To explore the training demand among clinical medical postgraduate students.Methods Focus Group Discussions and a research interview,two of the qualitative study methods were applied among medical students of a hospital.Results Different clinical medical postgraduate students need different levels training.The demand status related to the students' training styles.Students had desire to receive kinds of help of hospital administrative authority.Conclusion Training guide is importance during the growth of clinical medical postgraduate students.Hospital administrative authority should afford manifold ways to satisfy the demand of students for their training.
4.Evaluation of application effect of evidence- based nursing in reducing ventilator - associated pneumonia of patients with severe head injury
Yuhua ZHOU ; Yiying SONG ; Yibin LIU ; Meiqing XU ; Li LIN
Chinese Journal of Practical Nursing 2012;13(13):34-36
Objective To evaluate the application effect of evidence-based nursing in reducing ventilator-associated pneumonia (VAP) of patients with severe head injury. Methods 100 patients with severe head injury using mechanical ventilation were randomly divided into the control group and the observation group with 50 patients in each group.The observation group explored evidence from nursing of ventilator tube,oropharyngeal and sound sects care,airway care,balloon sleeves care to prevent biofilm on the endotracheal tube(ETT-BF) to fall off,enteral nutrition care,decubitus care,ward environment and the hand disinfection of medical staff and was given evidence- based nursing.The control group took routine care.The incidence of VAP,off-line time and mortality rate of the two groups were compared during the same time period. Results The incidence of VAP,off-line time,mortality rate showed significant difference between the two groups.The observation group showed better effect. Conclusions Implementation of evidence-based nursing can significantly reduce the off-line time of patients with severe traumatic brain injury using mechanical ventilation.It can decrease the incidence of VAP and mortality rate,improve the prognosis and reduce medical cost.
5.Use of electronic resources in readers of hospital library and measures for improving its information service
Xinyu TIAN ; Rui XU ; Yahan SONG ; Shumei YIN ; Yuhua WANG
Chinese Journal of Medical Library and Information Science 2014;(12):52-54
The use of electronic resources and needs of novel services in readers of our library and their suggestions for library website construction were investigated with questionnaires and suggestions were put forward for improving and deepening the information service in hospital library.
6.Clinical study on depression of aged patients with cerebral stroke hemiplegia convalescence
Haiyun ZHOU ; Yan FANG ; Qingsong YANG ; Fengjiao DONG ; Yuhua SONG
Chinese Journal of Postgraduates of Medicine 2011;34(1):16-20
Objective To evaluate the effects of Taoist cognitive psychotherapy on depression of aged patients with cerebral stroke hemiplegia convalescence. Methods Seventy-seven hemiplegia convalescence patients with depression were divided into general treatment group (38 patients, received general back-up psychology therapy) and combined treatment group (39 patients, received general back-up psychology therapy and Taoist cognitive psychotherapy) by random digits table. All patients were treated for 8 weeks and followed up for 6 months. Two groups were evaluated with HAMD and SS-QOL before treatment and at the end of the 2 weeks,4 weeks, 8 weeks and 6 months after treatment. The results were analyzed with statistics. Results In general treatment group, the HAMD scores were gradually decreased, and the HAMD scores of patients after 8 weeks' treatment were significantly lower than those before treatment[(22.35 ± 4.69)scores vs. (29.62 ± 5.95 ) scores,t = 6.425 ,P < 0.01]. At the end of 6 months after treatment, the scores increased [(24.48 ± 4.12 ) scores vs. (22.35 ± 4.69 ) scores, t = 2.014, P < 0.05], but they were lower than those before treatment(t = 4.836, P < 0.01 ). At the end of 6 months after treatment, the SS-QOL scores were lower than those after 8 weeks' treatment (t =2.543,P <0.05),but they were higher than those before treatment (t = 2.790,P < 0.05 ). In combined treatment group, the HAMD scores decreased continuously,and the scores after 8 weeks' treatment [(20.08 ± 4.60) scores] and 6 months' treatment [( 15.21 ± 3.42)scores] were significantly lower than those before treatment [( 30.14 ± 4.92 ) scores] (t = 8.341,15.443, P <0.01). Meanwhile,the HAMD scores after 6 months'treatment were significantly Iower than those after 8 weeks' treatment (t =4.724,P < 0.01 ). The SS-QOL scores after 8 weeks' treatment [( 117.56 ± 26.22)scores] and 6 months' treatment [(126.57 ±21.82) scores] were significantly higher than those before treatment[(86.54 ± 23.90) scores] (t = 6.716,8.916,P < 0.01 ) ,and there was significantly difference(t=2.378,P < 0.05). The HAMD scores of combined treatment group after 8 weeks' and 6 months' treatment were significantly lower than those of general treatment group at the same time(t = 2.118, P < 0.05 ;t = 8.405,P< 0.01 ) ,and SS-QOL scores were significantly higher than those of general treatment group at the same time (t = 3.123,P < 0.05 ;t = 6.580,P < 0.01 ). Conclusions General back-up psychology therapy combined with Taoist cognitive psychotherapy can improve depression and life quality of cerebral stroke hemiplegia convalescence in aged patients. The effects of Taoist cognitive psychotherapy is slower, but it is more beneficial in the long time.
7.Improving Design of Multichannel Digital Temperature Detecting System
Ping CHEN ; Meisheng SHI ; Zhenxing SONG ; Yuhua MENG
Chinese Medical Equipment Journal 1993;0(05):-
Objective To solve problems existing in multichannel digital temperature detecting system,including boring wiring,unreliable data storage,poor anti-jamming.Methods Dynamic address code design and automatic compositor algorithms were integrated into the software design,while the running out was eliminated by adopting watch dog chip.Results The validity of the improvement were proved by high & low temperature tests.Conclusion The improved system,with convenient wiring,reliable data storage,accurate results and short response time,can be applied to fully digital & unattended temperature detection.
8.Circadian rhythms and hematopoietic tumors
Kefu WU ; Xiaotong MA ; Guoguang ZHENG ; Yuhua SONG
Journal of Leukemia & Lymphoma 2013;22(2):81-84
The disturbance of life rhythm exerts negative influence on modem life.The relationship between the disruption of circadian rhythms and tumor growth has attracted much attention.It has been studied in breast cancer and prostate cancer,and chronotherapeutics has been used for treatment of some cancers in clinic.Researchers have begun to investigate the association of circadian rhythms with leukemia/lymphoma outside China.The literatures were reviewed and the launch of research on this field was appealed.
9.Human endogenous retroviruses and tumors
Kefu WU ; Guoguang ZHENG ; Xiaotong MA ; Yuhua SONG
Journal of Leukemia & Lymphoma 2014;23(3):137-140
Viral etiology of tumor has been studied for a century.Seven human tumor viruses were identified over the past half century.The complexity and regularity of the pathogenic mechanisms of human tumor viruses have been elucidated preliminarily.Human genome sequencing indicated that 8 % of human genes were composed of human endogenous retroviruses (HERVs).Owing to the findings that adult T-cell leukemia virus (HTLV) can cause human adult T-cell leukemia and animal exogenous and HERVs can induce tumors and malignant diseases,the relationship between HERVs and human tumors has attracted much attention receutly.Here this article discusses the association of the expression of HERVs with leukemia and solid tumors progression,which may be an important aspect of tumor virology.
10.Tumor microevolution and its clinical significance
Kefu WU ; Guoguang ZHENG ; Xiaotong MA ; Yuhua SONG
Journal of Leukemia & Lymphoma 2014;23(10):577-580
As novel organisms,tumors undergo microevolution in limited time and space in vivo.Due to genetic mutations and epigenetic mechanisms,tumor cell clones undergo linear or branching evolution.In most clinic patients,tumors develop through branching evolution pathways,causing tumor heterogeneity and affecting tumor progression.Microevolution is the biological basis for the refractoriness and recurrence of tumors and has been paid attention in research and clinic recently.In this paper,acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS) are used as examples to demonstrate tumor microevolution and the concept of linear and branching evolution.Questions and perspective in the field of tumor microevolution research are also discussed.