To study the effect of losartan on high glucose up-regulated expression of parathyroid hormone-related peptide (PTHrP) and its type 1 receptor (PTH1R) in NRK-52E cells. The expression of PTHrP and PTH1R were detected by RT-PCR and Western blot in control group (0 mmol/L glucose) ,normal glucose group (5 mmol/ L glucose) ,moderately high glucose group (16.7 mmol/L glucose) ,high glucose group (25 mmol/L glucose) ,and after intervention by 10 μmol/L losartan for 72 h (only Western blot). The expression of PTHrP and PTH1R were up-regulated by high glucose (PTHrP mRNA : 0. 66 ± 0.08, 0. 84 ± 0. 13,1.57 ± 0. 15, and 1.73 ± 0.21 ; PTHrP protein :0.63±0.12,0.68±0.06,1.02±0. 11, and 1.04±0.08 ; PTH1R mRNA :0.26±0. 08,0.28±0.07,2.35± 0. 10,and 2.47±0. 05 ; PTH1R protein:0. 88±0. 05,0. 87±0. 10, 1.05±0. 11, and 1.12±0. 09) ,and losartan inhibited the effects of high glucose (PTHrP 0.74±0. 15, PTH1R 0.98±0.06, both P<0.01). The results suggest that losartan could inhibit the expression of FTHrP and PTH1R induced by high glucose in NRK-52E.

Objective To study the role of parathyroid hormone-related peptide ( PTHrP) in transdif-ferentiation of NRK-52E cells induced by high glucose. Methods Expression of PTHrP and its receptor 1 in NRK-52E cells incubated with 0 ( control group) ,5 ( normal glucose group) and 16. 7mmol/L glucose ( high glucose group) ,respectively,was detected by RT-PCR and Western blotting,respectively. Expression of TGF-?1,MMP2,type Ⅰ collagen and ?-SMA in NRK-52E cells of normal and high glucose groups was detected by Western blotting and ROS was detected by CM2H2DCFDA after 72 h. Results The expression levels of PTHrP,PTHrP receptor 1 ( PTH1R) ,and PTHrP mRNA were higher in high glucose group than in control and normal glucose groups ( P

Objective To study whether eicosapentaenoate can decrease the apoptosis effects in-duced by palmitic acid in INS-1 or not. Methods Based on different condition, there were four groups in this study, including control group , EPA group, palmitic acid group, combination of EPA and palmitic acid group. The grow curve was detected by MTT, and the expressions of bax were detected by Western blot.Cell apoptosis was detected by caspase-3. ROS was detected by CM2H2DCFDA kit after 48h. Result The grow curve of combination of EPA and palmitic acid group was higher than that in plamitic group[24 h :(37.33±1.15)OD vs (30. 79 ± 1.55 )OD, P < 0. 01 ;48 h:(31.50 ± 1.56)OD vs (23.94 ± 1.10)OD, P<0. 01] . Caspase-3 activity and ROS and the expression of bax and SREBP1c in combination of palmitic acid and T0901317 group were lower than those in palmitic acid group[(3566. 67 ± 305.51 )OD vs (4233. 33 ±416. 33)OD]. Conclusion EPA can inhibit apoptosis in INS-1 induced by palmitic acid.

Objective To observe insulin synthesis and secretion in INS-1 under high glucose, and to clarify the effect of PTH1R. Methods After successful construction of recombinant PTH1R-siRNA vectors in INS-1 cell, insulin secretion and intracellular insulin content of control group, siPTH1R-Negative control group, PTHrP group, and siPTH1R group under 25 mmol/L glucose were measured by radioimmunoassay in INS-1 cell. Intracellular calcium were detected by Fluo-3/AM and the capability of glucose transport was calculated by assaying the uptake of [3H]-2-deoxy-D-glucose in cells.Results Compared with control group, and siPTH1R-NC group, PTHrP group showed increased capability of insulin secretion; PTHrP group had higher intracellular insulin levels than others; PTHrP group showed increased intracellular calcium; the uptake of [3H]-2-deoxy-D-glucose under high glucose after 48h of PTHrP group was increased(all P＜0.01). Conclusion There is a close relationship between PTH1R activation and insulin secretion and synthesis, PTH1R activation may be one of the protective mechanisms in maintaining function of β-cell under high glucose.

Objective To study the relationship between fragile histidine traid (FHIT), pituitary tumor transforming gene-1 (PTTG-1) in thyroid tumor tissue. Methods The expression of FHIT and PTTG-1 were detected by immunohistocbemistry in 96 eases (56 carcinoma,40 adenoma). Results Compared with thyroid adenoma, the expression of FHIT decreased (P <0.01) ,PTTG-1 increased in thyroid carcinoma(P <0.01). The expression of FHIT is different in thyroid carcinoma in eancerometastasis to non-cancerometastasis (P < 0. 01), prognosis index (≥65) and prognosis index(< 65) (P < 0.01 and P < 0.05) ; There also was statistically significant differences between the expression of PTTG in thyroid carcinoma (P <0.05 and P <0.01). Conclusion FHIT and FTTG-1 may be an important reference significance in the differentiation of benign and malignant thyroid tumor tissue, and may serve as useful prognostic markers.

ObjectiveTo evaluate clinical significance of serum free light chains (sFLC) in diagnosis and response to the therapies of patients with multiple myeloma (MM).MethodssFLC (κ,λ and κ / λ ratio)were examed by immumoassay from 62 patients with MM at different stage. The results were analyzed associated with clinical data,and 35 cases of chronic renal failure(CRF)patients and 62 cases of healthy donors were taken as controls.ResultsMedium sFLC of normal κ value was (13.25±6.46) mg/L,λ value was (18.39±11.42) mg/L; and κ / λ ratio was (0.97±0.64) mg/L (range 0.33-1.61).sFLC κ and λ of CRF patients were (200.01±299.87) mg/L,(191.02±245.98) mg/L,significantly higher than that of the normal control group (t =-17.804,-16.894,both P ＜ 0.001),but the κ/λ ratio was at normal range (1.11±0.29).κ value range was at 16.20- 35 250 mg/L in newly diagnosed intact immunoglobulin MM patients with IgGκ,IgAκ and IgDκ type.The range of λ values was 15.70-4885 mg/L in IgGλ,IgAλ,IgDλ type,and κ/λ ratio was abnormal in 96.5 ％ (55/57) patients (＜0.5 or ＞1.5).The κ,λ value and κ/λ ratio were close to that of the normal after remmision.ConclusionsFLC ( κ,λ,and κ / λ ratio) are very good monitoring markers for MM.

Objective To investigate the effects of health education on diet and behaviors in stegmonth. Methods Three hundred and forty three primiparas were divided into the experiment and control group.The 163 primiparas in the experiment group took the courses in the pregnant women’s school and 180 primiparas in the control group did not.The differences of diet and daily behaviors in stegmonth were compared between the two groups.Results There were statistically significant differences in the rate of diet,health behaviors,breast feeding between the two groups(P<0.01).Conclusion The health education for the pregnant woman can enhance their sense of self care,change their wrong behaviors,and improve the rate of breast feeding.

Objective To study the relationship between VEGF-C,CD34 expression and thyroid papillary tumor tissue by combining assays of VEGF-C and CD34 in thyroid tumor tissue.Methods The expression of VEGF-C and CD34 were dectected by immunohistochemistry in 61 cases(thyroid papillary cancer 38.thyroid adenoma 23).Results VEGF-C level in thyroid papillary cancer(38.24±19.58)were significantly hisher than that in thyroid adenoma(16.49±9.25,P<0.01);CD34 in thyroid papillary cancer (23.68 ±9.07)/HP were higher than that in thyroid adenoma(18.70±6.34,t=4.9889,P<0.01).We found a significant relationship between the VEGF-C expression and cancerometastasis or tumor size(P<0.01;P<0.05).There was also a relationship between the CD34 expression and cancerometastasis or tumor size or age (P<0.01 or P<0.05).There Was positive relationship between the expression of VEGF-C and CD34(r=0.46.P<0.01).Conclusion It is suggested that combined detections of VEGF-C and CD34 may have important reference value on the differential diagnosis between thyroid papillary tumor tissue.

OBJECTIVE: To evaluate the bioequivalence of domestic and imported Fenofibrate capsules in healthy volunteers. METHODS: In double-period crossover study, 18 healthy volunteers received a single oral dose of domestic Fenofibrate capsule 200 mg (test capsule) and imported capsule 200 mg (reference capsule). The content of fenofibric acid in plasma was measured with HPLC. BECS pharmacokinetics program was used to calculate the pharmacokinetic parameters and bioavailability and to evaluate the bioequivalence of two preparations. RESULTS: The main pharmacokinetic parameters of domestic Fenofibrate capsule vs. imported Fenofibrate capsule were as follows: t1/2(21.34?3.31) h vs.(21.83?4.35) h, Cmax(7.31?2.65) mg?L-1 vs. (7.28?2.66) mg?L-1, tmax(4.72?0.57) h vs.(4.67?0.59) h, AUC0～72(170.09?54.06) mg?h?L-1 vs. (172.2?54.64) mg?h?L-1, AUC0～∞(188.56?55.27) mg?h?L-1 vs. (192.27?56.62) mg?h?L-1. The relative bioavailability F0～72 and F0～∞ of domestic Fenofibrate capsule were(98.87?6.76)% vs.(98.00?6.72)%, respectively. Non-parameter test of tmax and variance analysis and t-test of Cmax and AUC0～72 showed there was no statistical difference between 2 kinds of Fenofibrate capsules. The 90% confidential intervals of AUC0～72 and Cmax of test capsule were 83.3%～116.9% and 81.1%～124.4%, respectively. CONCLUSION: The domestic and imported Fenofibrate capsules are bioequivalent.

Objective:To prepare the monoclonal antibody(mAb) against human B7 1 and analyse its biological characteristics.Methods:The B lymphocytes hybridization technique was applied by using XG7 B7 cell,a multiple myeloma(MM) cell line transfected with human B7 1 gene,as immunogen;the specificity and the antigen binding activity of mAbs were identified by flow cytometry and Western blot analysis;its biological effects on human PBTC and human B lymphoma cell line were examined by 3H TdR incroporation and annexin V satining.Results:Four mouse anti human B7 1(B7 1) hybridoma(1F11,3H8,6H2,7B10) were obtained.They secrete continuosly and steadily specific anti human B7 1(B7 1) mAb and their subclasses belong to IgG1 and IgM respectively;three of four mAbs could inhibit the proliferation of response cells(the human peripheral blood T lymphocytes),stimulated by costimulatory molecule B7 1.Furthermore,it was found that these mAbs induced the apoptosis of human B lymphoma cell line,Raji,which express naturally human B7 1 molecule,by using annexin V staining analysis after 24 hours of mAb treatment.Conclusion:Sucessefully obtained four mouse anti human B7 1 functional monoclonal antibodies,which have a potential value in anti allogenetic graft rejection and in the therapeutic approach of B lymphoma.