Objective To investigate the therapeutic effect of Ganglioside(GM1) on acute cerebral infarction.Methods 60 cases with acute cerebral infarction were divided into two groups randomly.At the basic of routin treatment for the twe gyoups,GM1 group was treated with GM1 40 mg/d for 14 days and control group was treated with Citicoline 0.75 g/d for 14 days.The neurological deficit scores(NDS),activities of daily living(ADL) and adverse reactions were evaluated.Results After treatment,the scores of NDS and ADL were singnificantly decreased in twe groups than before treatment((P

The effects of ranitidine (Ran) on cultured myocardial cells of neonatal rats were observed. Ran 100 ?mol?L-1 took no effect on spontaneous beating rates of cultured myocardial cells, but it decreased the beating rates increased by histamine 10 ?mol?L-1 and did not inhibit the beating rates increased by isoprenaline 2?mol?L-1. Histamine 10?mol?L-1 increased the amplitude, maximal rate of rise and pro-longed action potential duration at 50% (APD50) and 90% (APD90) repolarization. The effect was abolished by Ran 100?mol?L-1. The results suggested that Ran may possess antiarrhythmic action induced by histamine's increase.

Objective To investigate the comparability of test seven tumor marks results of two electrochemiluminescence immu-noassay analyzer between Roche Cobas E411 and Cobas E601 .Methods According to United States Clinical and Laboratory Stand-ards Institute (CLSI) EP9-A2 document ,E411 was acted as comparison method (X) and E601 was as the experimental method (Y ) .Alpha fetoprotein (AFP ) ,carcinoembryonic antigen (CEA ) ,carbohydrate antigen125 (CA125 ) ,carbohydrate antigen199 (CA199) ,carbohydrate antigen153(CA153) ,total prostate specific antigen(TPSA) and free prostate specific antigen(FPSA) levels of patient samples were tested and the results were analyzed .Results The correlation between the two systems was good and the bias was acceptable in the medical decision level .Conclusion Two systems have good concordance in detecting the seven tumor markers ,w hich can meet the clinical need .

Aim To demonstrate the effects and mechanism of adenosine A1 receptor agonist R(-)-N6-(2-phenylisopropyl) adenosine(R-PIA) on high glucose(HG)-induced myocardial hypertrophy by in vitro cultured myocardial cells from neonatal rats.Methods The protein content was assayed by the method of Lowry. The expression of p-ERK1/2 and ERK1/2 was determined by Western blot.The [Ca~(2+)]I transient changes of cell loaded Fura-2/AM were measured by Till image system.Results 1 μmol·L~(-1) R-PIA and U0126 inhibited similarly HG-induced increase of the protein content and [Ca~(2+)]I transient along with the relative expression of p-ERK1/2.These responses were completely abolished by adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(CPDPX).Conclusion Adenosine A1 receptor stimulation significantly inhibits HG-induced myocardial hypertrophy by mediating ERK1/2 pathway and Ca~(2+).

Objective To compare the characteristics of digital subtraction angiography ( DSA) and compu-ted tomography angiography ( CTA) of moyamoya disease .Methods To analyze the characteristic of DSA and CTA in 29 cases with moyamoya disease ,including arterial occlusion ,arteriarctia,abnormal proliferation of vascular ,collat-eral circulation.Results There were no statistically significant differences between DSA and CTA in detecting arteri-al occlusion,arteriarctia(CTA 53 hemicerebrum,DSA 57 hemicerebrum)(χ2 =2.167,P>0.05),abnormal prolifera-tion of vascular (abnormal:CTA 41 hemicerebrum,DSA 45 hemicerebrum;normal:CTA 16 hemicerebrum,DSA 12 hemicerebrum)(χ2 =0.757,P>0.05).But there was statistically significant difference between DSA and CTA in detecting collateral circulation .Conclusion CTA is a good method to find out moyamoya disease .But DSA is better in discovery collateral circulation of moyamoya disease .

Objective To study the levels of serum C -reactive protein (CRP),plasma fibrinogen (Fib),D-dimmer(DD)in the first onset young patients with acute progressive cerebral infarction.Methods 42 first onset young patients with acute progressive cerebral infarction(PIS group),50 cases of non -acute progressive cerebral infarction(N -PIS group)and 90 healthy people(health control group)were enrolled.The levels of serum CRP, plasma Fib and DD were detected and compared.Results PIS group:CRP (3.764 ±0.832)mg /L,Fib (3.994 ± 0.851)g/L,DD (1.560 ±0.225)μg/mL;N -PIS group:CRP (2.573 ±0.657)mg/L,Fib (2.468 ±0.739)g/L, DD (0.740 ±0.162)μg/mL;health control group:CRP (1.725 ±0.326)mg/L,Fib (2.103 ±0.584)g/L,DD (0.450 ±0.131)μg/mL.The levels of serum CRP,plasma Fib and DD of PIS group were higher than the other two groups(CRP:PIS group vs.N -PIS group t =8.89,PIS group vs.health control group t =13.99,N -PIS vs.health control group t =8.55,all P <0.01;D -D:PIS group vs.N -PIS group t =23.82,PIS group vs.health control group t =29.46,N -PIS group vs.health control group t =12.59,all P <0.01;FIB:N -PIS group vs.health control group t =2.85,P <0.05,PIS group vs.N -PIS group t =10.06,PIS group vs.health control group t =13.48,all P <0.01).Conclusion The levels of serum CRP,plasma Fib and DD are related to acute progressive cerebral infarction.

Objective To compare clinical curative effects of two types of compression hollow screws in the treatment of femoral neck fractures. Methods A retrospective analysis was carried out on clinical records of 87 cases of femoral neck fracture from February 1996 to September 2004 treated with two types of compression hollow screws,that is,doubleheaded compression hollow screws(46 cases,Group A) and AO titanium compression hollow screws(41 cases,Group B).Results A follow-up was conducted for 3 months~8 years(mean,4.2 years).No statistically significant differences were observed between the two groups.The intraoperative blood loss was significantly less and the time to bone union was significantly shorter in the Group B than in the Group A(t=5.147,P=0.000;t=5.472,P=0.000).The surgical outcomes were classified as "excellent" in 34 cases,"good" in 8,"fair" in 1,and "poor" in 3 in the Group A,while "excellent" in 37 cases,"good" in 2,"fair" in 2,and "poor" in no cases in the Group B,without statistically significant differences between the two groups in the rate of "good" or "excellent" results(89.1% vs 95.1%,?~2=0.398,P=0.528).No femoral head necrosis or non-union was found in the Group B. Conclusions Both of two types of compression hollow screws have showed advantages of simplicity of performance,minimal invasion,short operative time,reliable fixation,and high success rate,being an ideal option for femoral neck fractures.

Objective To observe the effects of Coptidis Rhizoma on gastrointestinal motility functions, MTL and GAS in spleen-deficiency rats; To explore the dose-effect mechanism of “invigorating stomach” and “spoiling appetite”.Methods Fatigue and troublesome were used to establish exhausted spleen-deficiency models. 60 SD rats were randomly divided into blank control group, model group, positive medicine group, Coptidis Rhizoma high-, medium- and low-dose groups, with 10 rats in each group. Each medication group received relevant medicine for gavage, while the blank control group and model group received normal saline for gavage, once a day for 14 d. The general gastrointestinal motility functions, MTL and GAS of rats in each group were observed.Results Compared with blank control group, the gastric residual rate of model group was significantly higher; small intestinal propulsion rate, MTL, and GAS were significantly lower (P<0.05,P<0.01). Compared with model group, the gastric residual rate in Coptidis Rhizoma low-dose group was significantly lower; small intestinal propulsion rate, MTL and GAS increased significantly (P<0.05,P<0.01); small intestinal propulsion rate and MTL in Coptidis Rhizoma high-dose group were significantly lower (P<0.05).Conclusion High-doseCoptidis Rhizoma leads to “spoiling appetite”, and low-dose Coptidis Rhizoma leads to “invigorating stomach”, which may be related to adjusting gastrointestinal motility directly or adjusting the secretion of gastrointestinal hormones.

Objective To investigate the curative effect of magnesium ion on patients with severe craniocerebral injury. Methods According to the standards,80 patients with severe craniocerebral injury were chosen and divided into control group and experimental group randomly. The control group received routine therapy and the experimental group received routine therapy plus magnesium sulfate. Serum endothelin(ET) and Glasgow coma score(GCS)of all the patients were measured at different time respectively,and Glasgow outcome scale(GOS)of all the patients were determined after 3 months,and then these data were analyzed statistically. Results Compared with the control group, Serum ET in the experimental group were lower,GCS were higher,and GOS were better. All these differences were significant(P

AIM : To explore the effects of DECB (deproteinized extract of calf blood, DECB) on hypoxia-reoxygenation-induced injury in cultured cardiac myocytes. METHODS : By using cultured myocardial cells as models, thenumber of death cells, beating frequency and the content of LDH were observed after hypoxia-reoxygenation-induced injury. RESULTS : 0.1 and 0.3 g?L -1 of DECB decreased the number of death cells and the content of lactate dehydrogenase (LDH), and restored the cellular beating frequency in the supernatant. CONCLUSION : DECB can protect the cultured myocardial cells against hypoxia-reoxygenation-induced injury.