Objective:To explore the function of virtual reality in clinical laboratory equipment maintenance teaching depends on present condition of Luohe medical college. Methods: 90 college students from laboratory medicine department were selected as experiment target. They were divided into control group and experiment group and each group were 45 students. All the chosen students had no significant difference in subjects, ages and hobbies. Traditional teaching was used in the control group and virtual reality was introduced in the teaching of the experiment group. Results: Scores of written exam and training operation were analyzed with SPSS19.0. It showed that the scores of the experiment group were better than those of the control group. Conclusion: Application of virtual reality in clinical laboratory equipment maintenance teaching can improve students’ scores and effects.

Colorectal cancer is one of the most common malignancies with a high risk of liver metastasis in China. Compared with systemic chemotherapy, hepatic arterial infusion (HAI) transports cytotoxic agents directly into the hepatic artery, leading to the persistent level of medicine in tumor cells and the relative lower systemic concentration. With advances in HAI, this technique has been successfully used not only as the pre-and post-operative chemotherapy for the resection of colorectal liver metastases, but also as an ad-juvant chemotherapy after the colorectal radical surgery to prevent the liver metastasis. This review discusses the application and per-spective of HAI in the treatment of colorectal cancer liver metastasis.

Raf kinase inhibiting protein (RKIP)plays a vital role in various physiological processes and participates in multiple signaling pathways,with a close releationgship with tumor.The mechanism of RKIP down-regulation in neoplasms includes transcriptional regulation,methylation,acetylation,histone modifica-tion,microRNA regulation and NF-κB/Snail/RKIP loop regulation.

Henoch-schonlein purpura nephritis (HSPN)is the most common pediatric secondary renal glomerular disease.It could play an important role in the pathogenesis and progression of HSPN that regulatory T cells decline in the children and that the differentiation of Th17 cells are maybe not able to be effectively inhibited,leading to the insufficient immunosuppressive effect and the Th17 cells overexpression.We could further study the correlation among HPSN and the quantity and functional imbalance of Th17 cells and regulatory T cells to provide more theoretical basis for clinical treatment of HSPN.

BACKGROUND:Left ventricle volume or volume index and left ventricle mass or mass index are commonly used to evaluate left ventricle remodeling. Recently,real-time three dimensional echocardiography (RT3DE) has been attempted to evaluate left ventricle remodeling index (LVRI),and it is believed that LVRI can serve as new index to evaluate left ventricle reconstruction clinically. OBJECTIVE:To assess application of RT-3DE in evaluating left ventricular remodeling in patients with hypertension. DESIGN,TIME AND SETTING:Case-control observation was performed at the Department of Ultrasonography,First Hospital of Liaoning Medical University from September 2008 to September 2009. PARTICIPANTS:A total of 90 hypertension patients and 30 normal people were selected. The hypertension patients were divided into 3 subgroups according to interventricular septum,left ventricle myocardial thickness and end-diastolic diameter:normal configuration,concentric hypertrophy and eccentric hypertrophy groups with 30 cases in each group. METHODS:Three dimensional echocardiography of 90 patients and 30 controls was performed. Left ventricular end-diastolic volume (EDV),left ventricular end-diastolic epicardial volume (EDVepi) were measured by RT-3DE; left ventricular mass (LVM),left ventricular mass index (LVMI),left ventricular end-diastolic epicardial volume index (EDVI),LVRI were calculated and compared with two-dimensional echocardiography (2DE) Simpson biplane method. MAIN OUTCOME MEASURES:LVRI,LVMI,and EDVI detected by RT-3DE and 2DE Simpson biplane. RESULTS:LVRI,EDVI,LVMI measured by RT-3DE and 2DE were significantly different in hypertension patients (P 0.05). The LVRI measured by RT-3DE,2DE and EDVI,LVMI measured by 2DE were significantly correlated in normal control,normal configuration and concentric hypertrophy group (P 0.05). CONCLUSION:Real-time three-echocardiographic LVRI can serve as a new approach for evaluating left ventricular remodeling in clinic.

Objective To investigate the influnce of dexamethasone pretreatment on the expression of ICAM 1 and neutrophil infiltration in brain tissue of hypoxic ischemic neonatal rats Methods Forty eight neonatal rats were made to model with hypoxic ischemic encephalopathy(HIE) The control group and the dexamethasone group were respectively injected intraperitoneally with normal saline 10 ml/kg, dexamethasone 0 1 mg/kg before hypoxia ischemia;all animals were killed 24 hours after hypoxia ischemia Immunocytochemistry was used to determine the absorbency of ICAM 1 The damage grade of neurocytes and leukocyte infiltration were recorded for the histopathological study Results Compared to the control group,the mean absorbency(A) of ICAM 1, the damage grade of neurocytes and neutrophil infiltration of the dexamethasone group were all obviously decreased( P

Objective To investigate the effect of different administrations of insulin-like growth factor 1(IGF-1) on cerebral hypoxia-ischemia injury in neonatal rats.Methods Forty neonatal rats were divided into four groups: control group, nasal-treated group, intravenous-treated group and sham-operated group. The two treat groups were given IGF-12.5?g (dissolved in 0.1 ml NS) intravenously or by nasal immediately after hypoxia, respectively. Normal saline (0.1 ml) was used intravenously in control group and only disassociation of common carotid artery was performed in sham-operated group. The animals were killed 24 hours after hypoxia. The expression of caspase-3 protein was determined by immunohistochemistry method, also pathological change was studied under light microscope.Results Compared to the control group, the expressions of caspase-3 protein in treat groups were obviously decreased (all (P

Objective To investigate the different characters between conventional frequency audiometry (125 Hz～8 kKHz) and high frequency audiometry (9～16 kHz)in the normal youth and provide the references for the clinical application of the HFA.Methods 52 male and 48 female normal-hearing subjects were tested with both conventional-frequency (0.25～8 kHz) and high-frequency (10～18 kHz) audiometry.Results Data showed that no difference between male and female in different frequencies. However, the threshold of HFA went up gradually with the frequency increasing. The average threshold of HFA was significantly higher than that of CFA and speech frequency (SFA) and had a positive correlation with both CFA and SFA. Conclusion The results of this study suggest that HFA might be used as an early indicator for CFA and SFA hearing loss resulting from acoustic trauma, ototoxicity and presbycusis.

BACKGROUND: Studies confirm that ischemia/reperfusion (I/R) injury can induce myocardial apoptosis. The loss of mitochondrial membrane potential (MMP) after reperfusion is the inevitable pathway of apoptosis. Protection of MMP may reduce apoptosis.OBJECTIVE: To observe the effect of naloxone on MMP of hypoxic myocardial cells and apoptosis in neonatal rats, and investigate the protective effect of naloxone on hypoxic myocardial cells.DESIGN: Observation and controlled trial.SETTING: Department of Cardiology, the General Hospital of Chinese PLA.MATERIALS: Detection of apoptosis of myocardial cells was carried out in the Laboratory of Pathophysiology, General Hospital of Chinese PLA in December 2004. Ten neonatal rats were used, and detection of MMP of myocardial cells was carried out in the same laboratory in March 2006 and 20 rats were used. All the involved rats were provided by the Animal Center of the General Hospital of Chinese PLA on the day of birth, were involved in this trial. Reagents: Naloxone hydrochloride injection (0.4 g/L, Beijing Sihuan Pharmaceutical Factory, Batch No. 0206272); the lowest and essential medium (Dulbecco, DEME,GIBCO company); phosphate buffer solution and fetal bovine serum (PBS and FBS, SIGMA Company).METHODS: The neonate rats were cut open along the median line of chest bone after local sterilization with iodine tincture on the day of birth. 1/3 ventricular myocardium before cardiac apex was harvested. On the 4th day of the culture,culture flasks of cells in good growth status ( ＞ 106 cells/bottle) were selected and divided into 3 groups: control group (normal culture, n =3 bottles), hypoxia group (hypoxia/reoxygenation, n =15 bottles) and naloxone group (hypoxia/reoxygenation, and treated by naloxone, n =15 bottles). Three time points were set in hypoxia group and naloxone group according to different time of hypoxia and reoxygenation: hypoxia 2 hours/reoxygenation 0 hour; hypoxia 2 hours/reoxygenation 2 hours; hypoxia 2 hours/reoxygenation 4 hours, 5 bottles at each time point. In the hypoxia group, DEME medium, which was pre-filled with 0.95 volume fraction of N2 and 0.05 volume fraction of CO2, containing 0.01 volume fraction of FBS, was used and 0.95 volume fraction of N2 and 0.05 volume fraction of CO2 was also filled to replace the air in the culture flasks. The culture flasks were enveloped for incubation at 37 ℃. The cells in the hypoxia group were incubated at normal condition (0.95 volume fraction of air and 0.05 volume fraction of CO2) at set time. In the naxolone group, hypoxia/reoxygenation treatment was the same as above, and naloxone hydrochloride was added at the sametime and the final concentration of naloxone in culture flasks was 5 μmol/L (The volume for naloxone ≤ 0.5% of the total medium). In the control group, hypoxia/reoxygenation and naloxone treatment were not given, but the same volume of normal saline was added, and this time served as time point of hypoxia 0 hour/reoxygenation 0 hour. After intervention,myocardial MMP changes and apoptosis were detected with fluorescent staining-flow cytometer at different time after hypoxia/reoxygenation.MAIN OUTCOME MEASURES: ① MMP changes of hypoxia group and naloxone group at different time points; ② Comparison of survival, apoptosis and necrosis of cells at hypoxia 2 hours/reoxygenation 4 hours in each group.RESULTS: ① After hypoxia, MMPs of the cells in hypoxia group and naloxone group were decreased. MMP of the cells in the naloxone group was higher than that in the hypoxia group at each time point (P ＜ 0.01). The great amplitude of decrease of MMP occurred in hypoxia period, but not in reoxygenation period. ② At hypoxia 2 hours/reoxygenation 4 hours, the apoptotic and necrotic rates in the hypoxia group were significantly higher than those in the naloxone group [(9.88±0.98)% vs. (2.41±0.52)%; (5.10±0.29)% vs. (3.56±0.56)%, both P ＜ 0.01]. The apoptotic rate was significantly higher than the necrotic rate in the hypoxia group (P ＜ 0.05).CONCLUSION: Early application of naloxone can significantly alleviate and postpone the decrease of myocardial MMP after I/R, and reduce apoptosis and necrosis.

Objective To evaluate endoscopic ultrasonography (EUS) for diagnosis of gastric stromal tumors (GST) by analyzing EUS features of tumors with different invasive capacities.Methods Clinical data of 34 patients with GST were analyzed retrospectively.The tumors were classified according to Fletcher′s GIST biological behavior ranking system and the corresponding EUS features were analyzed.Results The mean maximal diameter of GST in this cohort was 6.7cm.The echo heterogeneity, big tumors size, irregular shape and ulceration were more common in tumors of high risk (P<0.05).Conclusion Tumor size, shape, echo quality and ulceration detected by EUS are useful features for pretreatment evaluation of GST, and can guide the choice of following managements.