OBJECTIVE: To observe the effects of naoluotong on dogs' cerebral blood flow. METHODS:The anaesthetized dogs were randomly divided into naoluotong (which was subdivided into high, medium and low dose subgroups) group, antigeron positive control group, naoxuekang group, and blank control group with 6 dogs in each group. The internal carotid artery flow (ICAF), blood pressure (BP), electrocardiogram (ECG) and heart rates of the dogs were determined at different time after administration with drugs. RESULTS:As compared with the blank control group, the high and medium dose of naoluotong could significantly increase dogs' cerebral blood flow and reduce cerebral vascular resistance yet without significant influence on arterial blood pressure, heart rates and ECG. CONCLUSION: Naoluotong can improve cerebral blood circulation.

A semantic predication network was developed by processing the documents on schizophrenia into se-mantic predication sets using SemRep, from which the core information was extracted to produce a graphic summary which was consisted of highly cohesive cliques.The automatic methods for summarizing biomedical documents were studied using the network properties combined with semantic information.The subthemes in the summary obtained by clustering were evaluated according to the clique co-node matrix and the contents of the summary were assessed according to the reference criteria.The accuracy was 0.93, the recall was 0.68, and the F-value was 0.79 for the summary, indicating that this method can effectively recognize the core information in documents and the semantic information in network graphics.

The construction plan of information service platform for biomedical industry groups was proposed, including expert intelligence database, special database, advanced information service platform and biomedical achievements transformation platform.The information ( including names, subject fields and research interests of experts) in expert intelligence database can be searched according to the subject fields and research interests of experts.The special database is consisted of database of special literature, genes, proteins, special drugs, achievements, patents and demands.The information service platform can provide literature retrieval service, literature novelty assessment service, data-mining service, information analysis service, decision-making support service, and competitive informa-tion service.The biomedical achievements transformation platform can provide service for transforming achievements and for solving difficulty demand problems.

〔Abstract〕 The paper briefly introduces the concept and development process of Electronic Medical Records ( EMR) , elaborates in-formation extraction of EMR as well as the methods assisting clinical decision, including machine learning, statistical learning and rule induction, etc.It describes the application of EMR assisting clinical decision in diagnostic criteria identification and clinical diagnosis ac-tivities, reflects its evidence-based significance.

Aim To demonstrate the effects and mechanism of adenosine A1 receptor agonist R(-)-N6-(2-phenylisopropyl) adenosine(R-PIA) on high glucose(HG)-induced myocardial hypertrophy by in vitro cultured myocardial cells from neonatal rats.Methods The protein content was assayed by the method of Lowry. The expression of p-ERK1/2 and ERK1/2 was determined by Western blot.The [Ca~(2+)]I transient changes of cell loaded Fura-2/AM were measured by Till image system.Results 1 μmol·L~(-1) R-PIA and U0126 inhibited similarly HG-induced increase of the protein content and [Ca~(2+)]I transient along with the relative expression of p-ERK1/2.These responses were completely abolished by adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(CPDPX).Conclusion Adenosine A1 receptor stimulation significantly inhibits HG-induced myocardial hypertrophy by mediating ERK1/2 pathway and Ca~(2+).

The changes of the growth characteristics and surface ultrastructure during longterm passages of Wg3h cells that have been transfected with PSV_2-neo (Wg3h-neo) were studied. The results showed that the growth and DNA synthesis rate were evidently higher in the transfected cells than in the parental wg3h cells. The saturation of the transfected cell growth density was also increased, but neither the parental and transfected cells formed cell colony in soft agar medium nor tumor growth in nude mice. There was no significant differences in morphology between the two cell types under light microscopy, however much more microvilli were seen on the transfected cell surface under scanning electron microscopy. Southern blot hybridization analysis indicated that the PSV_2-neo plasmids were integrated into the genome of the target cells. Our results demonstrated that the transfected cells remained as nonmalignant cells although some changes of their growth characteristics and surface ultrastructure appeared.

A large number of starring biomedical industry clusters are emerged in recent years with the develop-ment of biomedical industry. The information access approaches of users in biomedical industry clusters consisting of higher education institutions, scientific research institutions and enterprises, the types of biomedical information they need, the transformation ways of their achievements, and the major barriers of information access were thus in-vestigated in this paper with questionnaires. The different information behaviors were compared between the users of scientific research group and enterprise group. The characteristics of information need of users in biomedical indus-try clusters were summarized, and following suggestions were put forward, namely establishing information service platform, perfecting institution-oriented information service model, and improving information service system for bi-omedical industry clusters.

OBJECTIVE: To develop a method of HPLC/MS/MS to determine phenacetin and paracetamol in rat liver microsomal incubation system. METHODS: Samples were separated on XTerra MS C18 column, different ratios of methanol- 0.1% formic acid were used as the gradient eluent, and the flow rate was 0.2mL?min-1. The electrospray ion-quadrupole mass spectrometry and multiple reaction monitor were adopted to detect the concentration of phenacetin and paracetamol. RESULTS: The calibration curves were linear in the ranges of 45～9 000ng?mL-1(r=0.999 8)and 15.2～1 520ng?mL-1(r=0.999 6) for phenacetin and paracetamol respectively; The lowest limits of assay were 9ng?mL-1 and 10ng?mL-1.The average recoveries at three concentrations of phenacetin were (96.2?2.3)%～(98.3?2.4)% and those of paracetamol were (99.6?2.1)%～(100.2?2.6)%; RSD of the intra-day and inter-day were less than 5%. CONCLUSION: The method is rapid, sensitive and suitable for determination of phenacetin and paracetamol in rat liver microsomal incubation system.

The dynamic changes of serum gastrin (SG) levels were observed in rats during chemical induction of colorectal cancer by 1,2-dimethylhydrazine (DMH). It was found that the mean SG concentration in the induced rats was very significantly lower than that in the control rats (P0.05) from week 11 to 20 after induction;the mean SG concentration in the induced rats with colorectal cancer in Dukes A or B stage was very significantly higher than that in those without cancer or with cancer in Dukes C or D stage (P

This study was aimed to investigate Baishile Capsule on behavior, memory and expression of SYN I and SYNA in hippocampal CA3 region of depression model rats. SD rats were randomly divided into six groups, which were the control group, depression model group, fluoxetine hydrochloride group, and the Baishile Capsule group (2.88 g·kg-1, 1.44 g·kg-1, 0.72 g·kg-1). The chronic stress depression model rats were established by chronic and mild unpredictable stressors as described in the literature. In the model group, medicine was intragastricly administered once per day and continued for 21 days. In the control group and model group, same volume of distilled water was intragastricly administered. The open-field test, preference for 1% sucrose solution, Morris water maze, and rate of weight were carried out and observed. Immunohistochemistry and in situ hybridization were used in the observation of the expression of SYN I and SYNA in each group of model rats. The results showed that high-dosage Baishile Capsule can significantly increase the horizontal and vertical activities of score after two-week treatment (P< 0.01). The middle-dosage Baishile Capsule can significantly increase the horizontal activity of score after three-week treatment (P< 0.05). The rat's preference for sucrose solution was obviously increased in the high-dosage group after one-week treatment and in the middle-dosage group after three-week treatment (P< 0.01, or P< 0.05). The rate of weight of rats was obviously increased in the high-dosage group after two-week treatment and in the middle-dosage group after three-week treatment (P< 0.01, or P< 0.05). The high-dosage and middle-dosage Baishile Capsule can shorten EL, and significantly increase the number of target quadrant. The high-dosage Baishile Capsule can obviously shorten the target quadrant latency (P< 0.05). Compared with the model group, the immunohistochemistry and in situ hybridization showed high-dosage Baishile can significantly promote the expression of SYN I and SYNA in hippocampal CA3 region; and the middle-dosage group can enhance the expression of SYNA (P < 0.05, or P <0.01). It was concluded that Baishile Capsule can obviously improve the behavior and the expression of SYN I and SYNA in hippocampal CA3 region of depression model rats.