The purpose of this study was to reveal the current situation of health technology transfer in Shanghai hospitals,and to analyze the problems and needs.Questionnaire and interview were adopted for the study.We found that in the past 3 years,the transformation rate of patent technologies at Shanghai Tertiary Hospitals was 5.88％.The transfer routes mainly involves proactive enlist of enterprises by project leader,technology brokerage,exhibitions of patents achievements,friends' recommendation,etc..The main factors impacted the transfer included poorer quality of patent,lacking efficient management,and lacking channels for transfer.In order to improve the transfer of patents,hospitals need to provide information service,technology assessment,project packaging,patents application,consultant,and relevant trainings etc.Other things that could promote health technology transfer are to improve scientific research system,to enhance the quality of scientific achievements,and to develop technology brokerage.

PRL-3 is a key gene related to metastasis of colorectal carcinoma. However, it is known little about the possible regulatory mechanisms of PRL-3 gene expression. There were three possible promoter regions predicted by TRED, a promoter prediction software,which were all located in the upstream regions of PRL-3 gene. One of PRL-3 gene candidate promoters was located in the region of about -1kb upstream proximal to 5′ UTR of PRL-3 gene. Many possible transcription factor binding sites such as Snail, n-MYC, ARNT, E74A, NF-kappaB, NRF-2 and AML-1 were predicted in the region by Consite, a promoter analysis web system. Interestingly, a 5′ CACCTG 3′ core sequence and other related sequences of snail binding sites were found in promoter region of PRL-3 genes. Two PRL-3 gene promoters between -699 to 299 nt and between -642 to -383 nt were cloned into pGL3 vector with luciferase report gene. Both of them had promoter activities in four different cell lines including colorectal carcinoma cell lines SW480 and SW620, nasopharyngeal carcinoma cell line CNE2 and human embryo kidney cell line 293A. Interestingly, the luciferase activities of the short DNA fragmentations with Snail binding site′s core sequence 5′ CACCTG 3′ were higher than that of the longer one. PRL-3 promoter obtaining the 5′ CACCTG 3′ core sequence of Snail binding sites, was validated to bind to snail by chromatin immunoprecipitation (CHIP) analysis and electrophoretic mobility shift assay (EMSA) in SW480 cells. The data suggested that Snail was involved in regulation of PRL-3.

Objective Establishing the scientific research competitiveness evaluation index system of Shanghai hospitals.Methods Delphi method and Analytic Hierarchy Process were applied.Results Thirty-four experts were invited to provide evaluation index and weighted scores.The positive coefficient from two rounds of expert's consultation were 88.24％ and 94％.The expert's opinion of authority coefficient of each indicator's was greater than 0.7.The degrees of coordinative consistency was high.Kendall concordance coefficient was increasing with the increasing of consulting times.After two rounds of Delphi expert consultation,2 level-one indictors,10 level-two indictors and 47 level-three indictors were confirmed.Conclusions After two rounds of Delphi expert consultation,using all objective indicators,an Index System for research competitiveness evaluation in the hospitals in Shanghai has been created and could be used with scientific,objectivity and feasibility.

Objective To analyze the distribution of aminoglycoside acetyltransferases (aac) gene in Acinetobacter baumannii, and to establish a molecular biology method to detect the drug-resistance of the bacteria. Methods The aac genes of 42 isolated Acinetobacter baumannii were analyzed by drug susceptibility and multiplex PCR assay. Results The isolated bacteria were more resistant to Gentamicin (57%) and less resistant to Amikacin (29%). As to the bacteria resistant to at least one of the following aminoglycosides, Gentamicin, Tobramycin, Amikacin, aminoglycoside resistant genes were detected in all of pathogens except 2 special. Among them 6 strains with aac(6′)-Ih, 20 strains with aac(3)-Ia, 14 strains with aac(6′)-Ib and 14 strains were found to have both aac(6′)-Ib and aac(3)-Ia. Conclusion Acinetobacter baumannii isolated in this district are easily resistant to Gentamicin, and it is most caused by the enzyme encoded by aac(3)-Ia and aac(6′)-Ib.

Objective To investigate the clinical and laboratory feature of neonatal hemolytic disease (HDN)occurred in Qingdao caused by mother-baby ABO blood type disagreement.Methods Serum bilirubin (TBIL)test and micro column gel technology were used on 422 cases neonatal hemolytic disease children blood samples (collected from Jun.2013 to Feb.2015).Results There were 388 cases first-born children among 422 cases including 206 cases of type A and 216 of type B and the difference between them was not statistically significant (χ2 =0.24,P >0.05).The difference between male (218 cases)and female (204 cases)was not statistically significant (χ2 =0.24,P >0.05)too.The indirect bilirubin (IBI)increas-ing was more obviously.The peak level of serum bilirubin was 116~465 μmol/L and 256.5~342.0 μmol/L was 38.9% (χ2=0.24,P >0.05).162 cases reticulocyte count was increased nearly 38.5% (χ2 =75.62,P <0.05).RBC antibody release test and serum free antibody test were often positive and the percentage was 80.1% (χ2 =146.98,P <0.05).Conclusion The neonatal hemolytic disease may turn up in first-born children.The child with three positive test was more sensitive to neonatal hyperbilirubinemia.RBC antibody release test and serum free antibody test are often positive.It is important to make the early diagnosis and treatment as soon as possible for reducing the bilirubin encephalopathy.

Objective To investigate the accuracy of the plasma DNA concentration in evaluating the prognosis in patients with sepsis.Methods One hundred and sixty patients with sepsis were enrolled as the sepsis group (group SE).Another 109 patients without sepsis hospitalized during the same period served as the control group (group C).The venous blood sample was taken on admission for determination of plasma DNA concentration by polymerase chain reaction,C reactive protein (CRP) concentration by ELISA.APACHE Ⅱ score and SOFA score were evaluated at 24 h after admission.The 160 patients with sepsis were divided into two groups according to the result of prognosis:survival group ( n =103) and death group ( n =57).Results Compared with group C,the plasma DNA concentration,CRP concentration,APACHE Ⅱ score and SOFA score were significantly increased in group SE (P＜0.05).Compared with survival group,the plasma DNA concentration,APACHEⅡ score and SOFA score were significantly increased in death group ( P ＜ 0.05).The areas under receiver operating characteristic (ROC) curves of the plasma DNA concentration was significantly larger than those of APACHE Ⅱ score and SOFA score (0.81(95％ CI,0.74-0.88) versus 0.68(95％ CI,0.60-0.77),or 0.72(95％ CI,0.63-0.82)).Conclusion The plasma DNA concentration can accurately evaluate the prognosis in patients with sepsis.As compared with the plasma CRP concentration,APACHE Ⅱ score and SOFA score,the plasma DNA concentration is more accurate to evaluate the prognosis in patients with sepsis.

Objective To analyze the reliability and validity of an established Research Hospital Competitiveness Evaluation System.Methods To evaluate the research competitiveness of 34 tertiary hospitals and their departments in Shanghai using above system.The reliability and validity of the indicator system were analyzed with the Cronbach's alpha coefficient, and confirmatory factor analysis based on the structural equation model.Results After adjusting for structure of the index system via item parceling, the CFI, MFI, RMSEA AND IFI were 1.000, 1.009, 0.000 and 1.028, the Cronbach's alpha of whole set indicators system was 0.768.Conclusions The results suggested that the measured reliability and validity are both in good degree, survey results can reflect the actual competence of evaluated hospitals.

Objective To establish a risk warning model for surgical site infection(SSI), provide support for screening high risk population and finding suspected cases.Methods Clinical data of 5 067 patients who underwent abdominal surgery in 6 domestic hospitals from January 2013 to December 2015 were collected retrospectively, all cases were randomly divided into modeling group and validation group according to a 6:4 ratio, warning model was established by employing logistic regression, the area under the receiver operating characteristic curve (AUC) was used to evaluate discriminant ability of evaluation model, the maximum Youden index was as the optimum cut-off point.Results For the warning model of high-risk patients, AUC was 0.823, sensitivity and specificity were 78.81% and 74.33% respectively, positive predictive value and negative predictive value were 19.67% and 97.78% respectively.For the discriminant model of suspected infection cases, AUC was 0.978, sensitivity and specificity were 93.38% and 95.62% respectively, positive predictive value and negative predictive value were 62.95% and 99.45% respectively.Conclusion The early-warning model established in this study has better discrimination ability, which can provide a reference for the development of early warning and discrimination of healthcare-associated infection information system.

Objective To explore the effect of pithy formula teaching method on the education of stoma patients.Methods Totals of 63 stoma patients were randomly divided into control group (n=30) that received the routine method teaching , and test group ( n=33 ) that received pithy formula teaching method .Two groups were received a test after education.Results The score of test group was (83.13 ±8.42), which was significantly higher than that of control group (72.21 ±7.72),and the difference was statistically significant (t=6.96,P<0.05).Conclusions Pithy formula teaching method mobilizes the learning enthusiasm and improves the learning effect of stoma patients .

Objective To explore the organization and practice method of the discussion of difficult nursing cases .Methods Many methods were used such as establishing the rules of discussion , making full prepare in advance , establishing the procedure of the discussion and so on .Results Five discussion meetings of difficult nursing cases have been held within 2 years.It become one of the key nursing rules in the hospital . Conclusions The discussion of difficult nursing cases improved the quality of nursing and nurses ’ skills,and promote the exchange of new service and skills , which fully embodies the nurses ’ professional values .