Objective To observe and analyze the clinical effect of aerosol inhalation with budesonide suspension liquid drug in the treatment of children with acute infectious laryngitis.Methods 76 children with acute infectious laryngitis were divided into the observation group (n=38) and control group (n=38).Two groups of chil-dren were treated with conventional therapy,on the basis of conventional therapy,the control group was given dexam-ethasone aerosol inhalation,the observation group was treated with atomization of inhalation with budesonide suspen-sion liquid.The clinical therapeutic effects of the two groups were assessed.Results The total effective rate of the observation group was 97.37%,which was significantly higher than 76.32% of the control group,the difference was statistically significant (χ2 =19.392,P<0.05).The disappeared time of fever,cough,hoarseness,dyspnea,throat beep symptoms in the observation group was (2.21 ±1.12)days,(2.21 ±0.54)days,(1.21 ±0.33)days,(1.61 ± 0.81)days,(1.23 ±0.21)days,which were significantly shorter than the control group,the differences were statisti-cally significant (t=4.118,4.362,6.369,3.943,3.946,all P<0.05).The two groups had no obvious adverse reac-tion,The duration and hospitalization time of the observation group were (2.11 ±0.72)days,(3.09 ±0.54)days respectively,which were significantly shorter than the control group (t=4.966,5.795,all P<0.05).The satisfaction rate of the observation group was (91.23 ±4.52)%,which was significantly higher than the control group,the differ-ence was statistically significant (χ2 =4.203,P<0.05).Conclusion For the children with acute infectious laryngi-tis,inhalation with budesonide suspension liquid therapy can significantly improve clinical symptoms,with no adverse reaction,shorten the course of disease and hospitalization time,reduce the physical and psychological suffering of chil-dren,improve the clinical treatment effect,promote the healthy growth of children,it is worthy of clinical application.

Objective To explore the ideal resuscitation pressure for uncontrolled hemorrhagic shock in different ages of rats.Methods Ninety-six rats were divided into 3 groups by ages (6 weeks,16 weeks and 12 months representing pre-adult,adult and elderly rats,respectively).Uncontrolled hemorrhagic-shock model was established in all rats.Then every group was divided into 4 groups by the different target pressures (untreated group,40 ～ 50 mmHg group,＞ 50 ～ 60 mmHg group,and ＞ 60 ～ 70 mmHg group).We recorded the survival time and 24 h survival rate of rats in each group and compared the blood gas analysis,blood lactate and hematocrit (Hct) level in each group at 90 min of establishing model.Results Different ages had different optimal recovery pressure.The optimal target resuscitation pressures for 6 weeks,16 weeks and 12 months rats were 40 ～50 mmHg,＞ 50 ～ 60 mmHg and ＞ 60 ～ 70 mmHg,respectively.Under the ideal pressure they had a better survival rate and lower blood lactate level (P ＜ 0.01 or P ＜ 0.05).Conclusious Hemorrhagic-shock rats at different ages have different ideal target resuscitation pressures during active hemorrhage.

Objective: To evaluate the experience of surgical treatment of anastomotic recurrence following resection of esophageal carcinoma. Methods: Reoperation was performed in 11 patients with anastomotic recurrence. The surgical procedure performed included cervical esophagocolostomy in 5, cervical esophagogastrostomy in 3, esophagocolostomy and esophagogastrostomy at the top of chest in 2, respectively, and thoracotomy in 1. Results: There was one operative death with mortality rate of 9.1%. The cause of death was respiratory failure. 1-, 3-and 5-year survival rates were 77.8% (7/9), 44.4% (4/9) and 22.2% (2/9), respectively. Conclusion: Reoperation is still effective for some patients with anastomotic recurrence. Early diagnosis and prompt surgical treatment can improve the outcome.

Objective To investigate the variation of chemical characteristics with environmental factors and establish a relationship betweem morphological characters and chemical composition of Prunella vulgaris collected in different areas of China.Methods Twelve phenotypic traits and three chemical compositions were assessed in 28 populations of P.vulgaris collected from different locations in China.Results The variability ranges observed at phenotypic and chemical levels were polymorphic.According to the morphological traits,28 populations of P.vulgaris could be grouped into six clusters,and two morpho-types could be clearly distinguished.Perceptible differences could be discerned in the plant height,leaf length,corolla length,calyx length,fruiting spikes length,and maturity period.Based on three kinds of components including ursolic acid,total flavonoids,and total polysaccharides,all populations could be identified as four types.Cluster Ⅳ showing high content of ursolic acid,total flavonoids,and total polysaccharides could be utilized to develop superior derivatives.Conclusion The variation of chemical characteristics is influenced by the genetic and environmental factors,such as soil,climate,longitude,and altitude.It provides a solid basis for efficiently evaluating qualities and establishing good agricultural practices for P.vulgaris.

Background and purpose:Esophagorespiratory ifstula is often accompanied with life-threatening pulmonary infection. Though the pulmonary infection can be controlled temporarily by conservative medical treatment or stent placement, but patients have a poor quality of life and short survival time. This study was to investigate the effectiveness and security of surgical treatment for patients with esophagorespiratory fistula.Methods:We retrospectively analyzed the clinical data of 7 patients with esophagorespiratory ifstula after surgical treatment between Jun. 2009 and Oct. 2013 in Henan Province Chest Hospital. The causes were the following: esophageal cancers (4 cases), congenital ifstula (1 case), diverticulum (1 case) and trauma (1 case). All patients underwent surgical treatment through thoracotomy. Surgical treatment consisted of esophagectomy gastroesophageal anastomosis and pulmonary lobectomy in 2 patients, esophagectomy gastroesophageal anastomosis and tracheal ifstula repair in 1 patient, remnant stomach repair and pulmonary lobectomy in 1 patient, esophageal ifstula repair and pulmonary lobectomy in 2 patients and esophagotracheal ifstula double deligation in 1 patient.Results:There was no perioperative death. The incidence rate of postoperative complications was 57% (4/7). Two patients got severe pulmonary infection. One patient suffered from esophageal-tracheal fistula recurrence and underwent elective jejunostomy. One patient had thoracic cavity infection. Six patients resumed normal eating after postoperative recovery. Follow-up was acquired in all cases. Three cases with benign ifstula remained well without recurrence. Four cases with malignant ifstula had an average survival time of 18.8 months (11–28 months).Conclusion:Once congenital esophagorespiratory ifstula is conifrmed, surgical management is recommended as early as possible. The selective surgical treatment for malignant esophagorespiratory ifstulas according to patient’s condition could improve the life quality and lengthen the survival time.

Aim To study the characteristics of the binding reaction of Troxetutin with bovine serum albumin (BSA) by fluorescence and ultra violet-visible absorption spectra.Methods The quenching mechanism of the fluorescence of BSA by troxerutin was studied with fluorescence.To determine the dynamic quenching constants and static binding constants,the Stern-Volmer equation and the double reciprocal Lineweaver-Burk equation were applied. The number of binding site was calculated with double logarithmic equation and the main binding force was discussed by thermodynamic equations. The binding distance and energy transfer efficiency between donor (BSA) and acceptor (troxerutin) were obtained effectively quenched fluorescence of BSA via static quenching processes. The binding constant Ka was calculated to be in the order of 106,indicating a strong interaction between Troxerutin and BSA. The number of binding site was approximately equal to 1,the binding distance was 1.97 nm,the energy transfer efficiency was 0.529,and the binding force was mainly hydrophobic force.Conclusion Troxerutin effectively quenchs the intrinsic fluorescence of BSA via static quenching mechanism,and the binding is mainly driven by the hydrophobic interaction.

OBJECTIVETo investigate the original plant and dietotherapy history of Prunella vulgaris by textual researches, and provide a theoretical basis for clinical medication, exploitation and protection of wild resources.

METHODThe ancient literatures in the graphic, geographical distribution and morphological description of P. vulgaris were used in this studied.

RESULTThe varieties of P. vulgaris for therapeutic were no confusion. However, there has existed confusion between P. vulgaris and its confusable species (Ajuga ciliate, A. decumbens and Lagopsis supina) with appellations, morphological description and attached drawing of original plant.

CONCLUSIONThe original plant is originated from two species (P. vulgaris, P. asiatica). P. hispida was used as a certified medicinal material in Yunnan province from Ming Dynasty to modern time. The dietotherapy history of P. vulgaris in China can be traced back to Ming Dynasty or before.

China ; Drug Therapy ; history ; Drugs, Chinese Herbal ; analysis ; history ; therapeutic use ; History, Ancient ; Humans ; Medicine in Literature ; Prunella ; chemistry ; growth & development

OBJECTIVETo investigate the herbal medicine of Prunella vulgaris by textual researches, and provide a theoretical basis for clinical medication, exploitation and protection of wild P. vulgaris resources.

METHODTextual research on medicinal works of past dynasties and field work were adopted.

RESULTThe natural distributions of P. vulgaris were concentrated distribution in Sichuan province, Huaihe river basin, and Middle-Lower Yangtze river valley in Chinese history. The indications of P. vulgaris in ancient and modern times were basically identical. While there were difference between the medicinal parts, harvest period and processing methods existed difference between ancient and modern.

CONCLUSIONThree periods that whole grass of P. vulgaris as medicinal parts (from late Ming dynasty to late Qing dynasty and early stage of Republic of China), both whole grass and spicas as medicinal parts (from mid-term Republic of China to 1963), and the semi-maturity or maturity of spicas as medicinal parts (from 1963 to today) existed. The processing method for medicinal parts of P. vulgaris adopted sun drying and shady drying in ancient China, but only the sun drying was only used in modern times.

China ; Drug Therapy ; history ; Drugs, Chinese Herbal ; administration & dosage ; analysis ; history ; Herbal Medicine ; history ; History, Ancient ; Humans ; Prunella ; chemistry

Objective To analyze the prognosis of patients with pulmonary artery sling (PAS) combined with tracheal stenosis(TS) without tracheal intervention,and to discuss the method for improving the effect of treatment.Methods The clinical data of 17 children with PAS/TS (moderate or severe) who received treatment at Beijing Bayi Children's Hospital Affiliated to General Hospital of Bejing Military Command from October 2011 to July 2015 were retrospectively analyzed,and the relationship between the effect and prognosis of re-implantation of the left pulmonary artery (LPA) without tracheal intervention was analyzed.Results Seventeen patients received re-implantation of the left pulmonary artery without tracheal intervention.Extubation was successfully performed in 12 of the 17 patients and they healed.The remaining 5 patients received tracheal intervention after the first operation but they all died.Of those 5 patients,3 received tracheal stent implantation (1 died from necrotizing enterocolitis,2 died from infection and multiple organ failure),and 2 received traheoplasty (both of them died from infection and tracheal fistula).Respiratory symptoms were reduced or resolved in all survivors.Diameter/length (％) in survivors without tracheal intervention was significantly higher than those who received tracheal intervention [(10.14 ± 1.58) ％ vs.(5.72 ± 1.17) ％,t =3.600,P ＜ 0.001].Patients with PAS undergoing LPA re-implantation achieved a good outcome if the diameter/length(％) of the trachea was up to (10.14 ± 1.58) ％.Patients with PAS undergoing LPA re-implantation achieved a bad outcome if the diameter/length(％) of the trachea was below to (5.72 ± 1.17) ％.Conclusions Most of patients with PAS/TS undergoing LPA re-implantation without tracheal intervention have a good outcome.It is feasible for them to avoid tracheal intervention.Diameter/length (％) may be a reliable indicator for determining tracheal intervention in surgical management of PAS.

In the present study, 89 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in China during 2007 to 2012 were randomly selected from the GenBank genetic sequence database. Evolutionary characteristics of these isolates were analyzed based on the sequences of non-struc-tural protein 2 (Nsp2) and glycoprotein 5 (GP5). The genetic variations of the isolates were also compared with six representative strains. The results showed that a high degree of genetic diversity exists among the PRRSV population in China. Highly pathogenic PRRSV isolates, with a discon-tinuous deletion of a 30 amino acid residue in the Nsp2 region, remained the most dominant virus throughout 2007-2012 in China. Owing to the extensive use of representative vaccine strains, natu-ral recombination events occurred between strains. Three isolates-HH08, DY, and YN-2011-were more closely related to vaccine strains than the other isolates. Both YN-2011 and DY were the evolu-tionary products of recombination events between strains SP and CH-1R. The results of the present study provide useful information for the epidemiology of PRRSV as well as for vaccine development.