Objective:To explore the Chlamydia pneumoniae (Cpn) immunoglobulin (Ig) G positive rate , inflamma‐tory factors and blood lipid levels in patients with coronary heart disease (CHD) .Methods :A total of 50 CHD patients hospitalized in our hospital were selected as CHD group ,and 33 non‐CHD patients visited to outpatient clinic in same period were regarded as non‐CHD control group .Blood lipid ,inflammatory factor levels ,and positive rate of Cpn IgG were compared between two groups .Results :Compared with non‐CHD control group ,there were significant rise in levels of TC [ (3.96 ± 1.07) mmol/L vs .(4.75 ± 1.33) mmol/L] ,TG [ (1.05 ± 0.42) mmol/L vs .(1.67 ± 0.82) mmol/L] and LDL‐C [ (2.12 ± 0.47) mmol/L vs .(2.88 ± 0.86) mmol/L] , P < 0.05 all ;significant rise in levels of inflammatory factor :C reactive protein [CRP ,(3.85 ± 0.87) mg/L vs .(5.80 ± 1.60) mg/L] ,interleukin‐ 6 [IL‐6 ,(6.50 ± 1.53) mg/L vs .(15.80 ± 7.62) mg/L] and tumor necrosis factor‐α [TNF‐α ,(5.50 ± 1.60) mg/L vs .(16.10 ± 2.20) mg/L] in CHD group , P < 0.05 all ;positive rate of serum Cpn IgG in CHD group was significantly higher than that of non‐CHD control group (78.0% vs .21.2% ,P < 0.05) .Conclusion : In present study ,positive rate of serum Cpn IgG ,inflammatory factor and blood lipid levels significantly rise in CHD patients ; Chlamydia pneumoniae infection and dyslipidemia may have a synergistic effect ,cause occurrence and development of CHD in common .

Objective:To compare therapeutic effect and safety between atorvastatin and simvastatin treating angina pectoris of coronary heart disease (CHD).Methods:A total of 150 patients with chronic stable angina pectoris (SAP)of CHD were selected and randomly and equally divided into atorvastatin group and simvastatin group accord-ing to number table method.Changes of angina pectoris attack frequency,duration,degree of pain,nitroglycerin dosage and blood lipids were compared between two groups before and after treatment.All patients were followed up six months.Results:Compared with before treatment,there were significant reductions in angina pectoris attack frequency,duration,pain degree and nitroglycerin dosage after treatment 12 weeks in two groups,P<0.05 all;but there were no significant difference in above indexes between two groups before and after treatment (P>0.05 all). Compared with before treatment,there were significant reductions in serum levels of total cholesterol [ (5.62 ± 0.54)mmol/L vs.(3.68±0.41)mmol/L,(5.78±0.56)mmol/L vs.(3.33±0.43)mmol/L],triglyceride [(2.30 ±0.42)mmol/L vs.(1.62±0.34)mmol/L,(2.35±0.41)mmol/L vs.(1.68±0.69)mmol/L]and low density lipoprotein cholesterol [(3.56±1.22)mmol/L vs.(2.76±0.83)mmol/L,(3.48±1.21)mmol/L vs.(2.43± 0.72)mmol/L],and significant increase in level of high density lipoprotein cholesterol [(1.32±0.35)mmol/L vs. (1.58±0.34)mmol/L,(1.30±0.46)mmol/L vs.(1.60±0.45)mmol/L]in atorvastatin group and simvastatin group after treatment (P<0.05 all),but there were no significant difference in blood lipid levels between two groups after treatment (P>0.05);there was no significant difference in incidence rate of cardiovascular events (28.00% vs.25.33%,P>0.05)between two groups during follow-up.Conclusion:Both atorvastatin and simvasta-tin can effectively regulate the state of high blood lipid,and possess good effect and safety for angina pectoris of coronary heart disease.

Objective To explore and analyze the pathogenic bacteria distribution and its prognostic factors of pneumonia patients after chronic intracerebral hemorrhage. Methods 375 pneumonia patients after chronic intracerebral hemorrhage who were treated in the rehabilitation department of Qingdao Workers' Sanatorium of Shandong Provincial Federation of Trade Unions from July 2011 to July 2013 were chosen as the research objects. The clinical data of all the subjects were collected, and the pathogenic bacteria distribution and its prognostic factors were analyzed by SPSS 17.0 statistical package. Results Good prognosis rate of the pneumonia patients after chronic intracerebral hemorrhage was 85.60%, the bacterium was the main pathogenic bacteria, accounted for 42.51%. Multiariable logistic regression analysis showed that swallowing reflex disorder (OR=12.922,P<0.01) and the combination of respiratory system disease (OR=3.327,P<0.01) were risk factors for the prognosis of pneumonia patients after chronic intracerebral hemorrhage, and chest breathing training (OR=0.695,P<0.01) was the protection factor. Conclusion The prognosis of pneumonia patients after chronic intracerebral hemorrhage is tolerable. The gram-negative bacterium is the main pathogenic bacteria. The prognostic factors of pneumonia patients after chronic intracerebral hemorrhage is concerned with swallowing reflex obstacles, other combination of respiratory system diseases and chest breathing training.In the process of clinical medical, we should strengthen the chest breathing training guidance and improve the prognosis of patients, especially in high-risk groups.

Objective:To evaluate clinical significance of change of serum thyroid hormone level in patients with ST elevation myocardial infarction (STEMI) and old myocardial infarction (OMI) .Methods :Clinical data of 52 STE‐MI patients (STEMI group) and 48 OMI patients (OMI group) hospitalized in our department of cardiology were retrospectively analyzed .Automatic chemiluminescent analyzer was used to quantitatively measure thyroid hormone levels in two groups .Results:Compared with OMI group ,there were significant reductions in serum levels of free triiodothyronine [FT3 ,(4.45 ± 1.09) pmol/L vs .(3.03 ± 0.14) pmol/L] ,free thyroxine [FT4 ,(18.53 ± 3.89) pmol/L vs .(11.83 ± 0.53) pmol/L] and thyrotropic‐stimulating hormone [TSH ,(1.85 ± 1.04)μIU/L vs .(0.26 ± 0.13)μIU/L] in STEMI group ,P<0.01 all .Conclusion:Thyroid hormone levels of STEMI patients are significant‐ly higher than those of OMI patients , serum thyroid hormone levels may reflect severity of coronary vessel lesions in patients with myocardial infarction.

Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and high mortality.Early diagnosis is the key for HCC treatment.DNA methylation is one of the important mechanisms of epigenetic genetics.To investigate the mechanism of DNA methylation can provide a theoretical basis for the diagnosis of HCC,and the methylation level of the specific genes can also be used as the biomarkers of HCC for the early diagnosis and prognosis evaluation.

Objective:To construct human adipose-derived mesenchymal stem cells (hASCs)-biomate-rial mixture 3D bio-printing body and detect its osteogenesis in vivo,and to establish a guideline of osteogenesis in vivo by use of 3D bio-printing technology preliminarily.Methods:P4 hASCs were used as seed cells,whose osteogenic potential in vitro was tested by alkaline phosphatase (ALP)staining and alizarin red staining after 1 4 d of osteogenic induction.The cells were added into 20 g/L sodium alginate and 80 g/L gelatin mixture (cell density was 1 ×1 06/mL),and the cell-sodium alginate-gelatin mixture was printed by Bioplotter 3D bio-printer (Envision company,Germany),in which the cells’survival rate was detected by live-dead cell double fluorescence staining.Next,the printing body was osteogeni-cally induced for 1 week to gain the experimental group;and the sodium alginate-gelatin mixture without cells was also printed to gain the control group.Both the experimental group and the control group were implanted into the back of the nude mice.After 6 weeks of implantation,the samples were collected,HE staining,Masson staining,immunohistochemical staining and Inveon Micro CT test were preformed to analyze their osteogenic capability.Results:The cells’survival rate was 89%±2% after printing.Six weeks after implantation,the samples of the control group were mostly degraded,whose shape was irregu-lar and gel-like;the samples of the experimental group kept their original size and their texture was tough.HE staining and Masson staining showed that the bone-like tissue and vessel in-growth could be observed in the experimental group 6 weeks after implantation,immunohistochemical staining showed that the result of osteocalcin was positive,and Micro CT results showed that samples of the experimental group had a higher density and the new bone volume was 1 8%±1%.Conclusion:hASCs-biomaterial mixture 3D bio-printing body has capability of ectopic bone formation in nude mice,and it is feasible to apply cells-biomaterial mixture 3D bio-printing technology in the area of bone formation in vivo.

OBJECTIVEThis study aimed to improve students' ability in practical and theoretical courses of oral health education and to promote students' learning interest and initiative.

METHODSFourth-year students of the oral medical profession from 2006 to 2008 at Weifang Medical University were chosen as research objects for oral health education to explore the experimental teaching reform. The students were divided into test and control groups, with the test group using the "speak out" way of teaching and the control group using the traditional teaching method. Results of after-class evaluation of the test group, as well as final examination and practice examination of the two groups, were analyzed and compared.

RESULTSAfter-class evaluation results of the test group showed that the "speak out" teaching method was recognized by the students and improved students' ability to understand oral health education. The final examination and practice examination results showed that the score of the test group was higher than that of the control group (P < 0.01).

CONCLUSION"Speak out" teaching methods can improve students' ability for oral health education, in accordance with the trend of teaching reform.

Objective To evaluate the diagnosis and therapy of intestinal lymphangiectasia.Methods In this study 15 patients were admitted in our hospital during recent 7 years.Clinical manifestations included hypoalbuminemia,symmetrical edema,emaciation,diarrhea and lymphopenia.Lymphangiography,lympanscintigraphy and biopsy were performed for diagnosis.Therapy conducted included conservative therapy,low-fat and medium-chain triglycerides(MCT)diet,albumin infusions,diuretics,total parenteral nutrition and octreotide.Surgical therapy ineluded thoracic duct-vein anastomasis and segmental resection.Results In this group 8 patients receiving conservative therapy were followed-up from 1.5 to 7 years(average 2.5 years).Symptoms were alleviated in 6 patients.Seven patients underwent operative therapy,among them,4 patients received thoracic duct-exterior jugular vein anastomasis and followed-up from 1 to 5 years,with symptoms mitigated in 2 patients.3 patients underwent local intestinal resection,follow-up from 1 to 3 years found one patient was cured,one was improved,and 1 patient died 3 months afterthe operation. Conclusion Intestinal lymphangiectasia is rather rare and there was no definite and effective therapy.

Objective To investigate the effects of uncoupling protein 2 (UCP-2) gene a 45 bp insertion/deletion (Ins/Del) polymorphism in the 3'-untranslated (3'-UTR) of its exon 8 on macroangiopathy in diabetes mellitus.Methods A total of 182 patients were selected,80 cases with macroangiopathy( A group), 102 cases without macroangiopathy(B group) ,UCP-2 gene polymorphism was confirmed by electrophoresis after PCR with 3% agarose,then compared genotype and allele gene frequency. Results The 3'-UTR Ins/Del polymorphism of UCP-2 gene in A group ( II:6. 25% 、ID: 18. 75% 、DD:75.00% ) and B group( II:9. 80% 、ID:23.53%、 DD: 66. 67% ) had no significant difference ( P ＞ 0. 05 ), and there was also no difference of alleles frequencies in two groups ( I: 15.63 %、 D: 84. 37 % )and(I:21.57% 、D:78.43%)(P ＞0.05). Conclusion No relationship of the 3'-UTR a 45bp Ina/Del polymorphism in exon 8 of the UCP-2 gene was found with macroangiopathy in diabetes mellitus.

Objective To summarize the clinical characteristics of liver cirrhosis complicated with chylous ascites.Methods From July,2007 to November,2012,patients diagnosed as liver cirrhosis complicated with chylous ascites were retrospectively analyzed.Analyze the etiology,liver function,ascites,imaging examination,treatment options and progonsis of these patients.Differences in measurement data were compared with U test.Results A total of 34 cases were enrolled,male 27 cases and female seven cases,average age (51.7±12.5) years old.Hepatitis B (20/34,58.8％) was the most common etiology of liver cirrhosis.Child-Pugh grading was grade B (26 cases) and C (eight cases).The appearance of aseites of 17 cases was chylous and the serm-ascites albumin gradient (SAAG) was 18.8 (2.6 to 32.5) g/L.The SAAG of 27 cases (84.4％) was no less than 11.0 g/L.The ascites triglyceride (TG) level was 2.94 (0.26 to 16.75) mmol/L,the TG of 27 cases (84.4％) was no less than 1.25 mmol/L.The level of TG (2.66(0.26 to 16.75) mmol/L) of patients with SAAG over 11.0 g/L was significantly lower than that of SAAG lower than 11 g/L (7.07(2.26 to 15.67) mmol/L,U=24.00,P=0.02).The lymphatic scintigraphy indicated that imaging agent leaked into peritoneal cavity in 29 cases (85.3 ％).Direct lymphangiography revealed lymphatic vessel structure abnormality in 15 cases (60 ％).Twelve patients received conservative treatment and four patients were effective,13 patients accepted peritoneal-venous shunting and 12 patients were effective,11 patients with microsurgical treatrnent and seven patients were effective.Conclusions Lymphatic struture is a possible cause of cirrhosis patients with chylous ascites.The characteristics of chylous ascites in cirrhosis patients are still the same as the characteristics of portal hypertension ascites.The SAAG remarkably increases,and the the level of SAAG probably depends on TG level.Lymphoscintigraphy has great value on the determination of the presence of ascites.And the direct lymphangiography could help to reveal lymphatic vessel structure abnormality.