The severe hand,foot and mouth disease(HFMD) caused by Enterovirus 71 (EV71) has become a serious threat to children's health.It is an important issues which need to be faced and resolved by pediatrician and disease prevention and control system,especially pediatric critical care specialist.The pathogenesis of severe HFMD caused by EV71 has not been clearly defined so far,and the clinical treatmnent is controversial.So,to further explore the mechanism of EV71 infection in severe HFMD and explore new effective prevention and control methods,have important significance in reducing the mortality and improve the quality of life of patients with severe HFMD.

Objective To explore the effect and intracellular signal transduction pathway of insulin-like growth factor 1 (IGF-1) on the expression of stem cell factor (SCF) in spesis rats colonic smooth muscle cells (SMC).Methods The model of spesis rats was established,colonic SMC from spesis rats were cultured by enzymolysis and identified by α-actin immunofluoresence methods.Colonic SMC incubated with different concentrations (0,50,100 and 150 μg/L) of IGF-1 for different durations (0,12,24,36 and 48 h),and another SMC were pretreated with specific mitogen-activated protein kinase (MEK) inhibitor PD-98059 and phosphatidylinostiol 3-kinase(PI 3-kinase) inhibitor LY-294002 before IGF-1 induced.The expression of SCF in colonic SMC was examined by Western blot and quantitative reverse transcription polymerase chain reaction.Results (1) A very low level of SCF was expressed in colonic SMC cultured in bovine serum free medium.The protein and mRNA were 0.223 ± 0.047 and 0.149 ± 0.023 respectively.The expression of SCF protein and mRNA from SMC was increased to 0.398±0.033 and 0.243±0.013(P＜0.05) when concentration of IGF-1 was 50 μg/L.The final effective concentration of IGF-1 in vitro was 100 μg/L,the levels of SCF protein and mRNA were increased to 0.719±0.017 and 0.360±0.006(P＜0.05).(2) For the time point induced the SCF expression by IGF-1,the expression level of protein and mRNA in 0 h were 0.347±0.031 and 0.092 ±0.018 respectively.The time point of SCF peak level occurred at the 24 h,the expression level of SCF protein and mRNA were increased to 0.893 ± 0.049 and 0.396 ± 0.048 (P ＜ 0.05).(3) The IGF-1-induced SCF expression was reduced significantly by a pretreatment of PD-98059 (SCF protein and mRNA were reduced 37％ and 45％,P＜0.05),and LY-294002 had no effect on the expression of SCF (P＞0.05).Conclusion The expression of SCF from colon SMC in sepsis rat is stimulated by IGF-1 in both dose and time-dependent manners,in which ERKMAPK signal transduction may play an important role.

Objective To study the clinical experience of pure laparoacopic left hemihepatectomy. Methods Pure laparoscopic left hemibepatectomy was attempted in 10 patients including 3 cases of primary hepatic carcino-mma,3 cases of cavernous hemangioma of liver,2 cases of hepatic cyst,and 2 cases of calculus of intrahepatic duct. Results Fully pure laparoscopic left hemihepatectomy was accomplished for all 10 cases. Operation time was 190-340(253.0±51.2) min,blood loss was 200-800(342.0 ± 191.7) ml,and hospital stay was(5.2 ± 1.3) d . No com-plicatious occurred. Conclusion Laparoscopic left bemibepatectomy is safe and feasible, which can be considered asan excellent choice for selected cases.

Retinoblastoma 94(RB94)gene,a new tumor suppressor gene,is found in the past several years.It has been found that Rb94 gene has a more powerful antitumor effection compared with Rb110gene.After transfection,Rb94 gene could adjust telomerase activation,induce apoptosis and regulate cell cycle.It also has a synergistic action associated with radiotherapy or chemotherapy.

Objective To study the expression of Sox2 in human prostate cancer and pericarcinomatous tissue,and the relation between its expression and the pathological type and clinical stage of prostate cancer.Methods Immunohistochemisty,Western blot and reverse transcription-polymerase chain reaction (RT-PCR)were used to detect the expression of Sox2 in 86 cases of prostate cancer tissues and 40 cases of pericarcinomatous tissues.The relation between Sox2 expression and the pathological type and clinical stage of prostate cancer was analyzed.Results The expression of Sox2 in prostate cancer tissue (71.9％) was significantly higher than that in pericarcinomatous tissue (7.5％),and the difference was statistically significant (x2 =53.98,P ＜ 0.05).Sox2 expression in prostate cancer tissues was significantly related to Gleason score (x2 =19.49,P ＜ 0.05).The positive rate of Sox2 in lymphatic metastasis group and negative lymphatic metastasis group was 92.3％ and 73.3％ respectively,and the difference was statistically significant (x2 =3.95,P ＜ 0.05).While there was no significant difference among clinical stages (x2 =0.16,P ＞ 0.05).Moreover,Western blot and RT-PCR revealed that the expression of Sox2 in prostate cancer tissue was significantly higher than that in pericarcinomatous tissue (t =-2.93,P ＜ 0.05 ; t =3.29,P ＜ 0.05).Conclusion The high expression of Sox2 is closely related to the Gleason score and lymphatic metastasis of prostate cancer,but not to the clinical stage.The overexpression of Sox2 may play an important role in formation,differentiation and metastasis of prostate cancer.

0.05).Conclusion The polymorphism of rs2285676 in KCNJ11 may not be associated with T2DM in Hanethnic population in Chongqing.

Objective:To investigate the effect of EBV protein on the production of Immunoglobulin(Ig)by cultured B cells.Methods:The purified human umbilical blood B cells were treated respectively with UV and Heat-inactivated EBV and cultured in complete IMDM. CD5, CD3, CD4 and CD8 cells were measured by flow cytometric analysis. IgG and IgM in the supernatant were detected by ELISA. At the mean time, IgG and IgM in the supernatant of cultured EBV-transformed B lymphocytes were detected by ELISA,and compared with that of UV-inactivated EBV group at the same period.Results:In UV-inactivated EBV group,CD3, CD4 and CD8 T cells couldn’t be detected,CD5 + cells occupied about 42% of all detected cells at the 14th day; and at the 28th day, 47%.The IgG A value of the 10th、18th、22th and 26th day in UV-inactivated EBV group have a significant difference compared with that in the control group ( P

Objective To study the role of ERK signal pathway in the endochondral ossification of bone mesenchymal stem cells ,and to explore the mechanism of ERK signal pathway in persistent enhanced FGFR2 function on development of mice BMSCs by a knock‐in mouse model with the FGFR2S252W/+ .Methods Mice with neo‐FGFR2 gain‐of‐function mutation were mated with EII‐Cre mice .The genotype of generation mice were identified by PCR and divided into wild type group and mutant type group ac‐cording to their genotype .6 week‐old mice were sacrificed to receive bone mesenchymal stem cells .The western blot was used to compare the level of P‐ERK and ERK and the RT‐PCR was applied to detect the genes of Col2 ,Col10 ,OC ,OP in chondrogenic dif‐ferentiation medium of BMSCs .Then ,treatment of cultured BMSCs with PD98059 ,compare the changes of genes and utilize the in vitro culture of long bones detect the role of ERK signal pathway in the endochondral ossification by FGFR2 mutant .Results We successfully derive BMSCs from FGFR2S252W/+ mutant mice and found the activity of ERK signal pathway of FGFR2S252W/+ was en‐hanced .After been cultured in chondrogenic differentiation medium ,the expressions of the BMSCs mRNA of Col2 ,Col10 from mu‐tant group were decreased ,while the expressions of OC ,OP were increased .Those OC ,OP genes levels showed an increased treated by PD98059 .Using in vitro culture of long bones ,we found the retardation of total length growth of long bones has been rescued by PD98059 treatment ,suggesting that ERK signal pathways was responsible for the retarded long bone development in FGFRS252W/+mice .Conclusion The results indicate these effects are mediated by the ERK signal pathway .Furthermore ,the retardation of long bones has been recued by PD98059 treatment ,suggesting that ERK signal pathway is responsible for the retarded long bone devel‐opment in FGFR2S252W/+ mice .

Objective:To compare the detective effect between color Doppler twinkling artifact and B-mode ultrasonography for kidney stones in order to develop an ultrasonic model that can more easily and more accurately detect kidney stone.Methods: 9 patients with kidney stone who were confirmed by CT scan in recently were selected to accepted two methods (color Doppler twinkling artifact and B-mode ultrasonography) to detect kidney stone. The video fragments of upper pole, interpolar and lower pole were created by three radiologists and the results were diagnosed by them.Results: In dual kidneys of the nine patients, 32 stones with average size of (8.9±7.5) mm were found. The sensitivity, specificity, positive predictive value and negative predictive value of the B-mode ultrasonography were 71%, 48%, 52% and 68%, respectively, while those of color Doppler twinkling artifact were 56%, 74%, 62% and 68%, respectively.Conclusions: In the detection of kidney stones, B-mode ultrasonography is more sensitive when is used alone, but color Doppler twinkling artifact has more high specificity. The color Doppler twinkling artifact should be applied in more clinical practices, and a new commercial ultrasonography mode that used basic acoustic difference and is special for detection of kidney stone should be researched and developed so as to improve the detection of kidney stone.

Objective To observe clinical therapeutic effect of Yiqi Huayu Prescription for diabetes peripheral nerve and blood vessel pathological change. Methods One hundred patients with diabetes peripheral nerve and blood vessel pathological change were randomly divided into two groups. The patients in control group (n=48) were treated by routine treatment. The patients in treatment group (n =52) were treated by oral use of Yiqi Huayu Prescription and routine treatment. Their therapeutic effects were compared after three courses of treatment, which was 30 days. Results The total effective rate was 92.9% in the treatment group and 61.1% in the control group. There was significant difference between the two groups (P 0.05). The internal diameter of the dorsal pedal artery and popliteal artery and the peak value of flow rate of the treatment group were improved (P