Objective To study the clinical experience of pure laparoacopic left hemihepatectomy. Methods Pure laparoscopic left hemibepatectomy was attempted in 10 patients including 3 cases of primary hepatic carcino-mma,3 cases of cavernous hemangioma of liver,2 cases of hepatic cyst,and 2 cases of calculus of intrahepatic duct. Results Fully pure laparoscopic left hemihepatectomy was accomplished for all 10 cases. Operation time was 190-340(253.0±51.2) min,blood loss was 200-800(342.0 ± 191.7) ml,and hospital stay was(5.2 ± 1.3) d . No com-plicatious occurred. Conclusion Laparoscopic left bemibepatectomy is safe and feasible, which can be considered asan excellent choice for selected cases.

Objective To prepare optimization of notoginsenoside R1 chitosan nanoparticles,to provide a theoretical basis for clinical application of the drug.Methods Notoginsenoside R1 chitosan nanoparticles were prepared,HPLC method was used to detect the content of notoginsenoside R1 chitosan nanoparticles,preparation technology of nanoparticles were optimized by orthogonal experiment,and the optimized preparation technology of nanoparticles was verified.Results HPLC standard curve equation was A =911.49C -1803.4(r =0.999 9),linear range was from 25 to 900 g/mL.The intra day precision were 1.520%,0.884% and 0.969%(n =6),and the inter day precision were 1.591%,1.447% and 1.269%(n =6).The recovery rates of low,medium and high concentra-tions were (98.11 ±1.16)%,(101.27 ±0.59)% and (100.97 ±0.82)%.4 factors of orthogonal experiment:the concentration of chitosan,the mass ratio of drug and carrier,temperature and rotational speed,and 3 levels of each factor were selected.The average particle size,encapsulation efficiency and drug loading were selected as control indexes.The test results were determined by the method of comprehensive weighted scoring.The orthogonal design was designed according to L9(34)orthogonal design.The optimization process was 2% of chitosan concentration,20% of the weight ratio of drug and carrier,35 ℃ of temperature,600 r/min of rotational speed.According to the optimized process,the average particle size was (123.40 ±7.68)nm,the encapsulation efficiency was (58.41 ±1.59)%,and the drug loading amount was (10.46 ±0.53)%.Conclusion The optimized preparation process of notoginsenoside R1 chitosan nanoparticles is simple and easy to operate,the entrapment efficiency and drug loading amount were high. As a new dosage form,it has a good clinical application prospect.

Objective To investigate the intracellular signal transduction pathway of insulin promoting stem cell factor (SCF) expression in colonic smooth muscle cells (SMCs).Methods Colonic SMCs from SD rats were isolated and cultured, which were identified by α-actin immunofluorescence cytochemistry staining.SMCs were divided into 3 groups for experiment. ①Insulin concentration gradient group:Adding different insulin concentrations (0,2.5,5,20,40 and 80 mg/L) into SMCs culture and cultured for 16 hours; ② Insulin time point group:SMCs were cultured with insulin (40 mg/L) for different time durations (0,8,16,24 hours); ③ Signal pathway blocking group:SMCs were pretreated with LY-294002 or PD-98059,and then induced with insulin (40 mg/L) for 16 h.The proliferation of SMCs was examined by MTT method.The change of SCF expression was determined by Western blot and RT-PCR.Results ① Insulin at concentration of 5 mg/L remarkably promoted SMCs proliferation,and there was no significant difference in its effect with insulin concentrations at 20,40,80 mg/L (all P＜0.05).② Compared with no insulin adding group,low and medium dose of insulin (2.5,5,20 mg/L) already had a role in promoting SCF mRNA and SCF protein expression (all P ＜ 0.05). It reached maxium effect when insulin concentration was at 40 mg/L(P＜0.05),and there was no significant difference between insulin concentration at 40 mg/L and 80 mg/L (P＞0.05).③ Compared with time 0 hour,the expression of SCF increased after insulin worked on SMC (40 mg/L) for 8 hours (P＜0.05),and reached the peak after 16 hours (P＜0.05),and there was no significant difference between 24 hours and 16 hours (P＞0.05).④ Compared with blank control group and solvent control group,both insulin-induced SCF expression reduced after pretreated with LY-294002 or PD-98059 (all P＜0.05).Conclusions Insulin can promote SMCs proliferation,and in certain range the induction of SCF synthesis in colon SMCs is in dose and time dependent manners.The effect may be related to PI3K and MAPK signal pathway.

Objective:To investigate the development of vestibular system function in 7 to 14 years old normal boys and the ADHD boys;Methods:The present study involved two groups of subjects:148 ADHD boys(age range 7 years to 14 years) diagnosed with DSM-IV diagnostic criteria and 148 normal boys with the same age.Each group was divided into 4 clusters according to two years gap.All subjects were tested for the vestibular system function by the Vestibular Function Test System 2000/ ENG-V600 with the eye movement index.Results:From 7 to 14 years,the development of vestibular system function in normal boys had the characters as the description below.The optokinetic system function exhibited more development from seven years in the normal group.From 7 to 10 years,the time delay of saccade and anti-saccade in normal boys was significantly shorter(In the normal controls,the delay time of left saccade was 149?66 ms in 7-8 years old boys,108?64 ms in 9-10 years old boys,117?72 ms in 9-10 years old boys,the delay time of left anti-saccade was 178?127ms in 7-8 years old boys,101?88 ms in 9-10 years old boys,P

Objective To evaluate the efficacy of supportive psychotherapy on patients with cardiac neurosis. Method 32 patients with cardiac neurosis were treated with a comprehensive measure consists of adequate drug therapy and supportive psychotherapy. Symptom Checklist 90 (SCL - 90) was rated. Result Among the 9 factors of SCL - 90, scores of somatization, obsessive - compulsive, interpersonal relationship, depression, anxiety and phobic anxiety factors were significanly higher than that of normal controls. After treatment, the scores of somatization, depression, anxiety and phobic anxiety were statistically reduced. Patient- reported improvement efficacy was 84. 38%.Conclusion Adequate drug therapy together with supportive psychotherapy has reliable efficacy on patients with cardiac neurosis.

0.05).However,GTPase activities of Wt PgFtsZ,Z?C01 and Z?N01 were 5.84?0.20,5.25?0.18,5.73?0.13,respectively,with 10 mmol?L-1 Ca2+,and reduced by 10 mmol?L-1 Ca2+(P

Objective To study application of surface plasmon resonance(SIR)system in detection of clinical pathogen with a gene chip.Methods 27 clinical samples were detected by SPR-based gene chip system.These samples were composed by 8 positive blood samples,3 positive pyoid samples,9 positive leucorrhea samples and positive reproductive tract pyoid samples,1 positive biopsy sample and 6 negative biopsy samples.Specific primers and probes for target pathogens were designed by bioinformatics methods and validated by PCR and enzyme-labelled chemiluminescence,respectively.SPR-based gene chip was prepared and utilized to detect clinical samples by SPR system.Results The primers and probes showed good specificity and accuracy,which can be applied to perform PCR and application of the gene chip.Compared with the clinical analysis,gene chip analysis of 26 clinical samples showed the consistent results.Conclusions SPR detection system proved to be accurate and reliable.The chip will have a promising prospect in application.

Objective To investigate the composite of chitosan(CS) and polyvinyl alcohol (PVA) as scaffold carrier for rabbit chondrocytes nurture and growth.Methods The third passage of chondrocytes were seeded in CS/PVA gel scaffold and 24,48 and 72 h after which cytoactive and toxicity were determined by MTT respectively.After one,two and three weeks,the growing status and morphology of chondrocytes in CS/PVA gel were observed with scaning electron microscope (SEM) and laser confocal scanning fluorescence microscope (LCSM).Results The third passage of chondrocytes in CS/PVA gel scaffold remained high proliferation ability.MTT measuring cell activity and virulence,the result showed that the number of cells obviously increased with the time,with statistical significance of difference between each groups (P＜0.05),without side effect to cells by the material.Observation of scaning electron microscope and confocal laser scanning fluorescence microscope showed that chondrocytes grew well with the scaffold of CS/PVA gel.Conclusion CS/PVA mixed gel material can be used as scaffold for rabbit chondrocytes growing for repairing cartilages defect in tissue engineering.

Objective To select the optimal exacting process for Chuanping prescription (CP).Methods The anti- and the anti- asthma ticanti effects of extracts obtained by different extracting processes from CP were observed.Results The anti- asthmatic effect of extract obtained by ion exchange resin(IER) method was better than that by acid- liquid- extraction alcohol- precipitate method (ALEAP) (P