As compared with penetrating keratoplasty(PKP), deep lamellar keratoplasty(DLKP) offers the same effect for recovery of corneal clarity in patients who have corneal stromal abnormality and/or opacity with normal endothelium. Many technical advances have been made for carrying out DLKP during past decade. However, the current status of the surgery is still technical difficulty, time consuming, possibly incomplete removal of recipient stroma or perforation of Descemet's membrane. As there may be further improvement of the technique, DLKP can be mastered by more ophthalmologists to treat patients who are currently considered as candidates for PKP. (Ophthalmol CHN, 2006, 15:156-158 )

Objective To study the new nursing method of patients complicated with urinary fistula after gynecological malignancy operation. Methods Nursing measures were provided with sufficient nutritional support and prevention of urinary tract infection by perineal nursing, vaginal plugging by OB sliver,and using endovaginal catheter to 23 patients complicated with urinary fistula after gynecological malignancy operation. Results 18 patients recovered by treatment and nursing, 4 mild cases cured after discharge from hospital and 1 patient died of MODS and multiple metastasis. Conclusions Vaginal plugging by OB sliver, perineal nursing and sufficient nutritional support have positive effect for patients complicated with urinary fistula after gynecological malignancy operation.

Objective To explore the previous and current status of hypertension, hyperglycemia and hyperlipidemia in teachers of the primary and middle high schools of Wuhu city. Methods 2 026 teachers aged 30~85 yrs were cluster sampled from 10 primary and 10 middle high schools in WuHu city. They were investigated on their previous examination of blood pressure, blood glucose & lipids, and current prevalence of hypertension, hyperglycemia and hyperlipidemia. Results The previous detection rates of the blood pressure, blood glucose and the blood lipid were 65.24%, 35.21% and 37.84%, respectively. The female previous detection rate of blood glucose and lipids was significant higher than those of the male (?2=4.42,P=0.035;?2=5.42, P=0.020). The result of trends test showed that detection rates were increased with the age increasing. The prevalence of hypertension, hyperglycemia and hyperlipidemia were 21.39%, 3.21% and 37.90%, respectively, among the participations without previous detection for the blood pressure, blood glucose and blood lipid, the hypertension prevalence was significant difference between the male and female participations (26.29% vs 18.68%; ?2=5.58, P=0.018). The medication compliance rate of the hypertensions, hyperglycemia and hyperlipidemia were 70.75%, 70.74% and 15.18% and the control rates were 47.04%, 72.22% and 5.85%. Concludes It is important to strengthen the detection of the blood pressure, blood glucose and blood lipid in community population in order to prevent and control the cardiovascular diseases.

The long noncoding RNAs refers to RNA transcripts more than 200 nucleotides in length,and do not encode proteins.In the end of the 20th century,the lncRNAs were found to play crucial role in many important biological processes,including embryonic development,cell differentiation,species evolution,metabolism,and disease occurrence by the scientific community.Currently,multiple studies have indicated that long noncoding RNAs have participated in rheumatic diseases,immune response,immune cell differentiation,and dynamic balance of the immune system.Therefore,summary of the roles of lncRNAs in rheumatic diseases could be beneficial to understand the pathogenesis of autoimmune diseases.This review article attempts to highlight the recent progresses regarding IncRNAs studies and the relationship between long noncoding RNA and rheumatic diseases by taking systemic lupus erythematosus,rheumatoid arthritis,arthritis and other typical diseases as examples.

Proteome research has become a new hot spot in the post-genome era. High-resolution two-dimensional gel electrophoresis (2-DE), which provides the most comprehensive analysis system of the whole proteome, was highly improved in recent years. With the development of computer techniques, the powerful and user-friendly image analysis systems appeared to help high-throughput, large-scale proteomic studies. Using new generation two-dimensional image analysis software, ImageMaster 2D Elite, the 2D gels of proteins extracted from cultured Schwann’s cells were processed. The analysis procedure, including image acquirement, spot detection, match, background subtraction, pI/Mr calibration, analysis results report and database query, were reported and discussed.

Objective To investigate the distribution of Human Papillomavirus 16 (HPV16) infection and its mixed infection with other HPV subtypes in the Yunnan region. Methods 16 166 cases of women were tested using flow fluorescence Luminex technology. Results (1) HPV16 infection rate and mixed infection rate was 2.2%and 28.0%, respectively; (2) The most common type of HPV16 mixed infection was HPV52, followed by HPV33. The two kinds of mixed infection accounted for 39.8% of the total infection rate; (3) There was a significant difference between each age group of HPV16 mixed infection (Chi=26.39, <0.01) . Conclusion The HPV16 infection was mainly HPV infection in Yunnan region. HPV16 mixed infection merged mainly with HPV52 and HPV33. HPV16 mixed infection was associated with age.

Objective To investigate the effect of immediate breast construction using prosthesi after nipple-areola-sparing mastectomy.Methods The immediate breast construction using prosthesi after nippleareola-sparing mastectomy was performed in 26 cases with breast cancer from January 2008 to December 2011 in Jiangsu Cancer Hospital.The postoperative cosmetic results and the complications were observed.The therapeutic effects were followed up.Results All operations were successful.The s,uperior rate of cosmetic result after one month according to JCRT was 88.5 ％ (23/26).No severe complication was found.After a median follow-up of 26 months (range 3-48 months),there was no recurrence and metastasis.Conclusion The immediate breast construction using prosthesi after nipple-areola-sparing mastectomy is maneuverable with satisfactory aesthetic result and the clinical effect,which deserves the further clinical application.

In recent five years,brain imaging studies suggested that internet addicts' neural pathway have abnormalities in reward circuits,executive control system,and decision-making system when they are in resting state or induced state.For internet addicts,in the aspect of reward circuits,they showed decreased metabolism level when undergoing a resting-state fMRI scan,and enhanced reward sensitivity as well as decreased loss sensitivity when functioning.In the aspect of executive control system,the related brain areas were associated with reduced white matter integrity and disrupted functional comnectivity in resting-state.When the task was internet-related,internet addicts showed enhanced executive control function.However,when the task was not internet-related,they showed reduced executive control function.In the aspect of decision-making system,reduced cortical thickness in related brain areas was found when internet addicts are in resting-state,and they possess high impulsivity and high risk tendency when they are in induced state.These findings are consistent with the conclusions of substance addicts which are based on the research of brain imaging,therefore,we preliminary think the internet addiction is a new type of addictive mental disorder.

MicroRNAs ( miRNAs ) play important roles in the processes of the occurrence and development of cancers , through regulating tumor related gene expression at post-transcription.It has shown that the expression of miR-214 is aberrant in cervi-cal cancer.Also,miR-214 could affect the proliferation, migration, invasion and apoptosis of tumor cells by targeting various genes . This article focuses on the studies of miR-214 function in cervical cancer , and will provide a novel approach for the clinical diagnosis and the treatment of cervical cancer .

Objective To investigate the functions of Rv1273c and Rv1272c, two ATP-binding cassette transporters (ABC transporters) in Mycobacterium tuberculosis (M. tuberculosis). Methods The ethidium bromide ( EB)-agar cartwheel and EB accumulation assays were used to detect the transport direc-tion of Rv1273c and Rv1272c in the recombinant strains of BL21/pET-28a-rv1273c and BL21/pET-28a-rv1272c, which were constructed by our lab in the previous study. RT-PCR was used to confirm whether rv1273c and rv1272c genes were in the same operon. Real-time PCR was performed to detect the expression of rv1272c and rv1273c genes in M. tuberculosis strains of H37Rv and H37Ra. Results Both the EB-agar cartwheel and the EB accumulation assays indicated that Rv1273c and Rv1272c transported EB into cells. Results of the RT-PCR showed that rv1273c and rv1272c were in the same operon. The expression of rv1273c and rv1272c at mRNA level in M. tuberculosis H37Ra strain were about 6 times higher than those in M. tuber-culosis H37Rv strain. Conclusion Rv1273c and Rv1272c are ABC importers and might be related to the virulence of M. tuberculosis.