Mesenchymal stem cells (MSCs) have a strong self-renewal capacity and multi-differentiation potential,which make them become ideal seed cells of tissue repair and target cells of gene therapy.However,the labeling and tracing method of MSCs transplanted in vivo is the hot spot and difficult problem of the current research.At present,the commonly used tracer methods of MSCs include fluorescence dye labeling,molecular marker,imaging marker technology.This paper summarized the advantages and disadvantages of these tracer methods.

Objective To produce humanized anti-keratin antibodies with high affinity. Methods Panning of phage antibody libraries against human epidermal keratin was conducted to select specific antibodies. Random mutation method and chain-shuffling technique for the gene of the antibodies were employed to improve antibody affinity. The binding activity, specificity and affinity of the obtained antibodies were de-termined and analyzed. Results Four clones of either IgG or IgM anti-keratin antibodies were seleeted from semisynthetic or natural antibody libraries. One of them was seleeted to undergo affinity maturation. Random mutation method for the gene did not result in any improvement in antibody affinity. By chain-shuffling technique, however, affinity level was markedly increased and an antibody with affinity of 8?1010/M was isolated. Conclusion It is feasible to generate humanized antikeratin antibodies using genetical engineering technique.

objective: To improve the repair technique of secondary nasal deformity following surgical treatment in the patients with bilateral cleft lip. Methods: 12 cases were treated with the operation technique: bilateral vermilion flaps were inserted into prolabial for the enlongation of upper lip and "V Y incision suture" principle was employed to repaire the nasal deformity. Results: Satisfactory results were achieved in all the 12 cases. Conclusion: The technique is feasible in the reconstruction of Cupid's bow, philtrum and correction of nasal and labia deformities.

Objective To study the protective function of ?-blockers to injury myocardial cell after PCI.Methods 60 cases were divided into two groups randomly,group A(30 cases) was control group,group B(30 cases) took ?-blockers orally before PCI.Serum levels of CK-MB were measured in 60 cases undergoigg coronary interventions in preprocedure,1st hour and 6th hour after operation,we judged the effect of ?-blockers.Results No difference was found in proprecedural level of CK-MB of two groups.There was significant difference between group A and group B 1h and 6h after operation(P=0.012 and 0.002).In the control group,we saw that the levels of postoperative CK-MB were higher than preprocedure (P=0.033 and 0.022).In hospital MACE did not occur in both groups.Conclution ?-blockers decrease the level of CK-MB in 30 cases undergoing coronary interventions.Thus it can decrease the mortality.

Objective To investigate the satisfaction degree of family members of children patients with the quality of nursing by follow-up analysis using telephone.Methods We used the high quality nursing questionnaire as the follow-up investigation.311 parents of the discharged pediatric patients were enrolled in this study,and answered the questionnaire within 1 ～2 weeks after their children were discharged from hospital.Meanwhile,we collected specific comments through asking questions by telephone.Then we analyzed and summarized the results by the content analysis.Results We found the total satisfaction rate was 96.65％ and there were three levels after qualitative analysis on the specific recommendations:evaluation of the hospital system,evaluation of professional knowledge and skills of the medical workers,evaluation of the attitude of the medical workers.Conclusions The parents of pediatric patients are satisfied with the high quality nursing which has pediatric features.However,what the parents focused on had already beyond the scope of the high quality nursing itself.We should expand the concept of quality service to every aspect of the hospital work,in order to meet the needs of the parents of pediatric patients in the department of pediatrics.

Objective To develop a sheet to assess infection risks in children with malignant tumors after chemotherapies.Methods A risk assessing sheet (to consult) was designed and used to consult 24 experts from 9 hospitals located in 6 provinces.After 3 rounds of expert consultation,a sheet (to try) was developed and tried by nurses in the clinic.And then a sheet (to use) was developed.Results The authority coefficient of experts was 0.79,the positive coefficient of experts was 1.00 and 0.96.The sheet (to try) developed after 3 rounds of expert consultation consisted of 30 items and was divided into 2 parts,one was about static items and the other was about dynamic items.Depending on the trying of the sheet (to try),the sheet (to use) was constituted by 8 fundamental immune state assessing items and 16 infection risk screening items.Conclusions Experts consulted in the study were quite reliable,representative and positive.The sheet (to use) seemed to have good pertinence and practicability.Further studies should be done to verify whether the sheet (to use) may help nurses to prevent children with malignant tumors from infections after chemotherapies.

BACKGROUND:Both osteopontin and leptin are closely linked to bone metabolism, therefore, which may be related to the attack of femoral head necrosis. OBJECTIVE:To study the correlation of serum osteopontin and leptin in the development of femoral head necrosis. METHODS:Thirty-one patients with femoral head necrosis (case group, including 11 cases of ARCO II, 10 of ARCO III, 10 of ARCO IV) and 10 healthy adults (control group) were selected as the research objects. Enzyme-linked immunosorbent assay was used to determine the levels of osteopontin and leptin in serum folowed by statistical analysis. RESULTS AND CONCLUSION:In the case group, the serum levels of osteopontin and leptin were significantly higher than those in the control group (P < 0.05). But the serum levels of osteopontin and leptin showed no correlation between the two groups. These findings indicate that the serum levels of osteopontin and leptin are both elevated in patients with femoral head necrosis, which maybe play a role in the pathogenesis of femoral head necrosis, but there is no obvious correlation.

Object To compare the contents of alkaloids of callus at different period with tissue culture of fresh bulbs of Fritillaria mellea S. Y. Tang et S. C. Yueh.Methods To study the best condition of induction frequency of callus about some phytohormones, temperature and light, and then to determine the contents of alkaloids of callus in F. mellea. Results The best condition of induction frequency of callus above was NAA 1 mg/L+ 6BA 3 mg/L; temperature at (22?1) ℃ and scattered light; the contents of alkaloids were kept highest in about 20th day ofter the callus appeared.Conclusion The contents of alkaloids of callus are kept higher level than fresh bulbs of F. mellea.

Objective To explore the effect of CYP2C19 polymorphism on plasma minimum concentration of Voriconazole in children with hematological malignancies complicated with invasive fungal infection.Methods Twenty children with hematological malignancies complicated with invasive fungal infection were selected from the Department of Pediatrics,the First Affiliated Hospital of Zhengzhou University were selected,and 5 mL venous blood for each was extracted.CYP2C19 genotypes of the whole blood of all patients were detected by using the method of polymerase chain-reaction restriction -fragment length polymorphism(PCR -RFLP).All the patients were treated with Voriconazole at the same time and by the same way.Plasma concentration of Voriconazole was measured by the method of fluo rescence polarization immunoassay.The impact of CYP2C19 genotypes on plasma minimum concentration of voriconazole was analyzed by using the rank sum test.Results Typing results showed that the incidence of iuhomozygous extensive me-tabolizers (EM)genotype (CYP2C19* 1 /*1 )was 30%(6 /20 cases);the incidence of mixed sub extensive metaboli-zers (IM)genotype (CYP2C19*1 /*2 or CYP2C19*1 /*3)was 45%(9/20 cases),among which ,CYP2C19*1 /*2 was in 4 cases,CYP2C1 9*1 /*3 was in 5 cases;and that of poor metabolizer (PM)genotype (CYP2C1 9*2 /*2 or CYP2C1 9*2 /*3 or CYP2C1 9*3 /*3)was 25%(5 /20 cases),among which,CYP2C1 9*2 /*2 was in 3 cases, CYP2C1 9*2 /*3 was in 1 case,and CYP2C1 9*3 /*3 was in 1 case.The serum trough concentration of Voriconazole in EMgroup,IMgroup and PMgroup was(2.30 ±0.50)mg/L,(3.23 ±0.71 )mg/L,(4.84 ±0.29)mg/L,respec-tively.There was a statistically significant relationship between CYP2C19 genotype and plasma minimum concentration of Voriconazole (F =26.99,P =0.032).Conclusions CYP2C19 polymorphism has a significant effect on the mini-mum concentration of Voriconazole in children with hematological malignancies complicated with invasive fungal infec-tion,which indicates that administration of Voriconazole for clinical treatment should be based on individual CYP2C19 genotype.

Objective To evaluate the performance of Mindray TSH chemiluminescence immunoassay ,including imprecision , limit of detection ,functional sensitivity ,interference ,cross-reaction ,and calibration consistency .The purpose was to confirm Mind-ray TSH assay meets the criteria of IFCC standardization and harmonization of thyroid functional tests .Methods The CLSI guide-lines defined in EP documents have been followed to measure the limit of detection ,functional sensitivity ,specificity ,imprecision and calibration consistency of Mindray TSH immunoassay on CL2000i system .Results Limit of detection was 0 .001 5 μIU/mL ,func-tional sensitivity was 0 .013 μIU/mL ,the correlation coefficient of linearity was 0 .999 in the range of 0-98 .95 μIU/mL .Mindray TSH calibrator C0 spiked with luteinizing hormone (LH) up to 500 mIU/mL ,follicle stimulating hormone (FSH) up to 500 mIU/mL ,or human chorionic gonadotropin (HCG) up to 200 000 mIU/mL detected no cross reactivity (detected TSH was less than 0 .2μIU/mL) .Hemoglobin up to 500 mg/dL ,bilirubin up to 10 mg/dL ,triglycerides up to 1 800 mg/dL ,and protein up to 10 g/dL showed -6 .91% ~8 .60% bias in TSH measurement .The total imprecision of two controls (high and low levels) and three serum specimens were in the range of 3 .24% and 5 .34% .Calibration consistency which had been demonstrated with high and low controls were measured between -6 .58% and 5 .26% .Conclusion The performance of Mindray thyroid-stimulating hormone assay meets the criteria for IFCC standardization and harmonization of thyroid function tests .