BACKGROUND:Scaffold materials are the important part of tissue engineering bone, and the ideal biodegradation speed of the scaffold materials can be in accordance with the regeneration speed of bone formation in order to achieve a long-term stability. OBJECTIVE:To summarize the biodegradability of different types of currently available scaffold materials and its relationship with bone formation. METHODS:The PubMed and CNKI database were searched by the first author using the key words of “tissue engineering bone, composite scaffold, scaffold degradation” in English and Chinese for articles addressing biodegradability of scaffold materials and its relationship with bone formation published during January 1996 and December 2013. RESULTS AND CONCLUSION:Many scaffold materials under organic, inorganic and organic-inorganic categories have been available and the current studies focus on inorganic materials and organic-inorganic composite materials.In vivo andin vitro studies indicate the different characteristics and relative merits of these scaffold materials. Organic materials have a relatively slow degradation rate while inorganic materials can completely degrade but have an insufficient mechanical property. For organic-inorganic composite materials, further studies are needed.

Objective:To evaluate the effect of segmental approach in the treatment of Angle class Ⅱ division 1 malocclusion.Methods:7 cases of classⅡ,division 1 maloclusion were treated.Upper first premolar and lower second premolars were extracted in all cases.Lower first molars were mesially moved by means of segmental arch. Cephalometric analysis was used to evaluate the effects of the treatment.Results:Facial profile improvement and lower anterior facial height maintenance were achieved.The occlusion plane angle,mandibular plane angle were well controlled with lower anterior teeth upright on the lower basal bone.All patients represented good mandible response.Conclusion:Segmental technique is a simple and effective approach in correcting class Ⅱ,division 1 maloclusion

Objective:To evaluate the reliability of localization of the impacted teeth by assessing various conventional radiographies. Methods:32 patients with impacted teeth were included. The impacted teeth were localized by conventional roentgenographic techniques including panoramic radiography (PR) and linear tomography (LT).Results:Dentomaxillary orthopantomographic solution (PR,LT and PR combined with LT) clearly presented the position of the impacted teeth. Serial cross-section linear tomography also ensured the shape and axis of the impacted teeth captured on the radiograph. The accuracy of the localization was further verified by the surgical exposure in 5 cases. Conclusion:Orthopantomographic assessing can directly and precisely localize the buccal and lingual position of the impacted teeth.

Objective To analyze the incidence of complications during and after interventional therapy for common con-genital heart disease (CHD) in children. Methods From January 2011 to December 2013, interventional therapy of common congenital heart disease which include ventricular septal defect (VSD), atrial septal defect (ASD), patent ductus arteriosus (PDA) and pulmonary valve stenosis (PS) were performed in 2356 patients. Among them, 159 patients who developed complications during and post to interventional therapy were retrospectively analyzed. Results The overall complication rate was 6.75%(159/2356) (11.40% post VSD occlusion, 7.50% post ASD occlusion, 3.09% post PDA occlusion, 1.63% post percutaneous balloon pulmonary valvuloplasty (PBPV) ).The rate of arrhythmia was 4.41%(102/2356). The severe complication rate was 2.71%(64/2356) (3.62%post VSD occlusion, 2.21%post ASD occlusion, 2.53%post PDA occlusion, 1.63%post PBPV). The intraoperative severe complication rate was 0.51%(12/2356);the early severe complication rate was 1.99%(47/2356);the late severe complication rate was 0.21%(5/2356). Interventional therapy rate was 0.13%(3/2356); cardiovascular surgery rate was 0.64%(15/2356);conservative treatment rate was 1.95%(46/2356). The mortality rate was 0.08%(2/2356). Conclusions The complications and mortality rate of interventional therapy for CHD in children are relatively low, but cannot be ignored. The complication could be reduced by choosing proper indications, following the operational procedures and careful operative follow-up.

Objective To observe and analyze the medium and long term follow-up data of arrhythmias after transcatheter closure of children with ventricular septal defect (VSD). Methods Retrospectively analyzed the clinical data of 1071 children with VSD, who successfully underwent transcatheter device closure, at l, 3, 6, 12 months and ev-ery year post procedure from March 2002 to December 2010. Results Of all 1071 children, 272 cases (25.4%) were ob-served of having arrhythmias within 1 month after intervention, mainly including atrioventricular block (AVB), branch block, junctional tachycardia, atrial and ventricular tachycardia, frequent contractions, etc. Among them, 22 cases (2.1%) had above II degree AVB, complete left bundle branch block (CLBBB) and other causes of serious arrhythmias. After treatment, all cases got better and no permanent pacemaker was necessary. After 1 to 107 months (2.8±1.7 years) fol-low-up, 161 cases (18.2%) were observed of having persistent abnormal ECG mainly caused by AVB and branch block, including 10 cases (1.1%) with serious arrhythmias. In 4 cases with late-onset AVB, 3 cases had already appeared AVB in early postoperative, 1 case had recurrence CLBBB, left ventricle enlarge, and died of heart failure during follow up. Four cases were implanted permanent pacemaker. Conclusion During follow-up, serious arrhythmias after VSD closure, such as AVB or CLBBB, have high risk of recurring. Conduction block arrhythmias may reappear or worsen, while arrhythmias like tachycardia and premature heart rhythm mostly return to normal.

[ ABSTRACT] AIM:To investigate the characteristics of the intestinal microbial flora in the pregnant women with congenital heart disease fetus ( PW group) and normal pregnant women ( NW group) .METHODS: Stool samples were collected from 15 NW and 17 PW cases.The bacterial genomic DNA was extracted.The 16S rDNA was amplified by PCR, and the second generation of Illumina sequencing was conducted.RESULTS: We obtained 2 696 276 ( NW group) and 2 445 530 ( PW group) optimized sequences.The coverage was greater than 97%.We obtained 77 243 operational taxono-mic units ( OTUs) in NW group and 75 600 OTUs in PW group after a 97%similarity merge.In NW group, the Chao 1 in-dex and the Shannon index were greater than those in PW group.The diversity analysis of microbial population indicated that they were mainly composed of Firmicutes, Proteobacteria and Actinobacteria.In family, the Bifidobacteriaceae and Cori-obacteriaceae were significantly different through analysis of variance.CONCLUSION: The Bifidobacteriaceae and Cori-obacteriaceae may play an important role in the occurrence of congenital heart disease.

Objective To assess the effect of transcatheter closure of patent ductus arteriosus (PDA) by comparing five different devices.Methods 354 patients (135 males, 219 females) underwent transcatheter closure of PDA using the Porstmann Ivalon plug, Rashkind double umbrella, Sideris button device, Coils and Amplatzer occluders.The mean PDA diameter at its narrowest segment was 3.1 ± 1.3 mm. Qp/Qs was 2.2 ± 0.5. Follow-up evaluation was performed with color flow mapping of the main pulmonary artery at 24 h and 6 months after closure.Results Successful occlusion was observed in 92％ (23/25) for the Porstmann device, 98.7％ (77/78)for the Rashkind device, 100％ (43/43) for the Sideris device, 100％ (69/69) for the Coil and 100％(139/139) for the Amplatzer device. No residual shunts were needed for the Porstmann, while 1.4％ -15％ of patients who received the Rashkind, Sideris, coils or Amplatzer needed residual shunts during follow-up. There were complications of 24％ (6/25) for the Porstmann, 3.8％ (3/78) for the Rashkind,1.4％ (1/69) for Coils and 1.4％ (2/139) for the Amplatzer. No complications were found in patients with the Sideris device.Conclusion The results showed that the Sideris device, coils and the Amplatzer device are more effective and safer than the others for the occlusion of PDA in children.

Objective To investigate the prevalence of GJB2 ,SLC26A4 and mitochondrial DNA 12S rRNA m .1555A>G(mtDNA 1555A>G) mutations in Hui ethic group patients with nonsyndromic hearing loss (NSHL) from Northwest China .Methods A total of 420 peripheral blood samples were collected from unrelated Hui ethic group probands with NSHL in Northwest China .Amplified the target gene by polymerase chain reaction (PCR) af-ter extracting genomic DNA from whole blood .The mtDNA 1555A>G mutation was detected by PCR -Alw26I di-gestion ,then direct sequencing was used to the positive samples of mtDNA 1555A> G ,the coding region of GJB2 gene ,exon 8 and 19 of SLC26A4 gene .Results There were 11(2 .62% ) cases caused by mtDNA 1555A>G homo-zygous mutation in 420 patients with NSHL .There were 41(9 .76% ) cases including homozygote and compound het-erozygote ,caused by GJB2 gene mutation ,which was the most frequent deafness -related gene .The allel frequency of c .235delC accounted for 6 .90% ,as well as the most frequent(51 .33% ) mutational pattern in GJB2 gene .There were 20 patients(4 .76% ) were found carring two allel mutations in SLC26A4 gene .The allel frequency of c .919 -2A>G was 5 .0% ,accounting for a total of 68 .85% in all base alterations of SLC26A4 gene ,which was the major mutant form of SLC26A4 gene .Conclusion GJB2 gene is the most common deafness -gene in Hui ethnic group pa-tients with NSHL from Northwest China ,while c .235delC is the main mutant form ,and c .919-2A>G is the hot-spot mutation of SLC26A4 gene .Through this study we can provide the molecular epidemiology basis for Hui ethnic group patients with NSHL from Northwest China in genetic diagnosis ,genetic counseling and therapy by associated testing of three frequent hearing loss genes .

This study is designed to theoretically evaluate the treatment effects of MEAW with tip back bends on the mandible dentition when used as a finishing archwire without elastic and with long class III elastics respectively, and to compare them with those of stainless-steel wire and shape-memory wire. The finite element analysis (FEA) method was adopted and the findings were as follows: (1) In the case of no elastics, the MEAW rotates the second premolar, the first molar and the second molar distally while rotating other teeth mesially, and depresses anterior teeth. However, the stainless-steel wire and the shape-memory wire rotate molars distally while rotating other teeth mesially. Furthermore, they extrude anterior teeth and depress posterior teeth. (2) The MEAW with tip back bends and long class III elastics inclines and rotates posterior teeth more distally than the MEAW with only tip back bends does. In the case of tip back bends and long class III elastics loaded together, the shape-memory wire inclines teeth in greater strength as compared with the stainless-steel wire. (3) The stress level of tooth root is the lowest with MEAW, the highest with stainless-steel wire, and is middle with the shape-memory wire. From these results, it is suggested that: (1) The MEAW therapy technique is effective for leveling the curve of Spee and regulating tooth respectively. (2) The MEAW therapy technique can transfer therapy force efficiently. (3) The MEAW is considered to be suitable for treating openbite malocclusion to make the posterior teeth upright because it effectively rotates teeth distally through the force of posterior bends and long class III elastics. (4) The loaded force on the teeth is more soft and permanent in the MEAW than in the stainless-steel wire and the shape-memory wire.
Biomechanical Phenomena ; Finite Element Analysis ; Humans ; Imaging, Three-Dimensional ; Malocclusion ; therapy ; Orthodontic Appliance Design ; Orthodontic Brackets ; Orthodontic Wires ; Orthodontics, Corrective ; methods ; Tooth Movement Techniques ; instrumentation

Objective To investigate the related factors of sarcopenia(SP)in hospitalized male patients with type 2 diabetes mellitus(T2DM).Methods 800 male patients with T2DM who were hospitalized in our department from August 2019 to June 2022 were enrolled in the study.All the subjects were divided into SP group(n=305)and T2DM group(n=495),according to the diagnostic criteria on sarcopenia.General data,biochemical indicators and bone metabolism data were collected.Body composition was determined by dual energy X-ray absorptiometry.Results BMI,HbA1c,FIns,TG,FC-P,HOMA-IR,fat content in Android area,fat content in Gynoid area and A/G fat ratio in SP group were lower than those in T2DM group(P<0.05).The incidence of DM complications was higher in SP group than those in T2DM group(P<0.05).After adjusting for age,BMI,DM complications and the level of 25(OH)D.Logistic regression analysis showed that A/G fat ratio was the influencing factor of SP.Conclusions A/G fat ratio is a protective factor for SP in hospitalized male T2DM patients.Early screening and intervention in fat distribution may be effective measures to delay the occurrence of SP.