Objective To evaluate the clinical value of tumor chemosensitivity test in the guidance of the advanced epithelial ovarian carcinoma patients treated with chemotherapy.Methods Women with ovarian cancer were enrolled and fresh tissue samples were collected for chemoresponse testing.Oncologists chose a drug for each patient according to the patient’ s condition by double blind method.Each treatment was classified by the assay as: sensitivity (S); moderate sensitivity (I);resistant (R).Progression-free survival (PFS) and overall survival (OS) were detection.The relationship between treatment response and PFS or OS was analysis.Results 262 patients were enrolled.The PFS and OS were significantly improved in sensitivity patients (S) after chemotherapy,but no significant difference were found in I and R groups.In I+R groups (HR=0.66,P=0.008) , median PFS was 8.9 months, OS was 5.8 months.Chemotherapy response test results was consistent with clinical results cisplatin sensitive and resistant ( HR:0.72 vs.0.66).Multi factor regression analysis showed that tumor chemosensitivity test was independent prognostic factors (HR =0.66, P =0.021).The average OS extended for 14 months (37.6 months for the S group vs.group I +R 23.8 months,HR =0.62, P =0.011).Conclusion Tumor chemosensitivity assay is correlated with the clinical effects and might play an important role in guiding the recurrent epithelial ovarian cancer patients with individualized chemotherapy.

OBJECTIVE To investigate and analyze the cause of the cross infection of Methicillin-resistant Staphylococcus aureus(MRSA) in ICU patients,and to apporach its preventive and control measures.METHODS Eiological inspection and sanitary monitoring have been done in ICU where there were two MRSA patients from June 6th,2008 to August 1st,2008.RESULTS MRSA has been detected on the hands of the ICU medical staff,on the bed-desks,on the switches of the vacuum extractor and gas supply unit on the wall,and in the NS for inhaling sputum.The separation rate were 37.5%,20.0%,54.5%,37.5%,87.5%.CONCLUSIONS ICU patients are prone to be infected with MRSA,which has a strong drug resistance and the infection is difficult to be treated.The cross infection can be prevented and controlled by rational administration,sterile operation and insulation.

Objective To assess the effect of fructose sodium diphosphate and alpha-lipoic acid on diabetic peripheral neuropathy. Methods Sixty-six patients with diabetic peripheral neuropathy were randomly divided into two groups, additional to the regular treatment to control blood glucose, 34 patients in the treatment grouptreated with fructse sodium diphosphate 10 g and alpha-lipoic acid 0.45 g daily through intravenous drip for two weeks (3 patients dropped out due to un-tolerance), 32 patients in the control group treated with alpha-lipoic acid 0.45 g dai-ly through intravenous drip for two weeks. All patients were examined on the improvement of symptoms, motor and sensory nerve conduction velocity of the median nerve and nervus peroneus communis before and after the treatment. Results After treatment for two weeks, the overall effect rate in the treatment group was 93.55 % (28/31), which was significantly higher than that in the control group(68.75%,22/32) (P < 0.05). In the treatment group, the motor nerve conduction velocity for the median nerve and nervus peroneus communis was 47.7 (SD:3.8) m/s and 41.6 (SD: 3.3) m/s after treatment, which was significantly higher than that before treatment (42.5 [SD:2.5] and 36.0[SD:2.8] m/s,respectively) (P <0.05). In the control group, the motor nerve conduction velocity for the median nerve and nervus peroneus communis was 43.4 (SD: 2.0) m/s and 39.6 (SD: 3.3) m/s after treat-ment, which was significantly higher than that before treatment (41.9 [SD16.0] and 36.2 [SD: 1.9] m/s, respec-tively) (P < 0.05). In the treatment group,the sensory nerve conduction velocity for the median nerve and nervus peroneus communis was 40.3 (SD: 1.8) m/s and 34.1 (SD: 2.3) m/s after treatment, which was significantly higher than that before treatment (35.2 [SD:2.3] and 28.8 [SD:2.5] m/s,rospectively) (P <0.05). In the control group,the sensory nerve conduction velocity for the median nerve and nervus peroneus communis was 37.7 (SD:1.7) m/s and 30.8 (SD:2.6) m/s after treatment,which was significantly higher than that before treatment (34.8 [SD :3.1] and 27.8 [SD :2.4] m/s, respectively) (P < 0.05). Furthermore, the improvements in the treat-ment group was significantly higher than those of the control group (P < 0.01). Conclusions Fructse sodium si-phosphate combined with alpha-lipoic acid is more effective to treat diabetic peripheral neuropathy than alpha-lipoic acid only.

Objective To determine the relationship between the content of microelement and the health of children.Methods Atomic absorption spectrometry,aas.Children were divided into 3 groups:physically weak group,minimal brain dysfunction(MBD) group and control group.Peripheral blood gathered from all the children.Put 40?l of blood sample into the specific reagent,detect the content of zinc(Zn),cuprum(Cu),ferret(Fe),calcium(Ca),magnesium(Mg),lead(Pb),cadmium(Cd).The result was compared between the 3 groups.Results Lack of Zn,Fe,Ca and Mg mostly be detected in physically weak group(P

The antimicrobial activity of nitropropane compound 791224 against several phytopachogenic fungi and Fusarium was studied in vitro. Its inhibitory effect on mycelial growth of Fusarium oxysporum f. sp. vasinfectum (Atk.) Snyder et Hansen and Fusarium graminearum Schwabe showed a much stronger than that on the other phytopathogenic fungi tested. The inhibition of 791224 on all tested Fusarium showed excellent. Its inhibitory effect values ranged from 54.2 to 100% at 5 ppm for 12 tested Fusarium whereas the respective values of carbendazim ranged from 0 to 27.3%. The inhibitory effect of 791224 at 5 ppm on Fusarium lateritium Nees reached to 100% whereas for carbendazim it was 0, i.e.100 times lower than that of 791224. In green house, the control efficiency of 791224 on Fusarium solani sothora japonico H. has been tested and showed a promising result.

[Objective] We assessed in a retrospective unicenter study the state of metabolism and gonadal axis of early menarche girls and girls who treated with Gonadotropin-releasing hormone analogs (GnRHa).[Methods] Thirty-nine early menarche girls and 58 girls who had been treated with GnRHa were enrolled in our study and 19 normal menarche girls were enrolled as control group.Data were collected in height,weight,gonadal hormone,blood glucose,insulin,blood lipid,leptin,adiponectin and the size of uterus and ovary.[Results] Both BMI SDS for chronological age (CA) and for bone age (BA) of early menarche girls were significantly higher than normal menarche girls (P ＜ 0.05).The ratio of insulin resistance in early menarche girls (20.5％) was also significantly higher than normal girls (0％).No significant difference in lipid metabolism and gonadal axis between two groups.In girls treated with GnRHa,BMISDS,insulin,HOMA-IR and the ratio of insulin resistance (20.7％) were all significantly higher than normal group (P ＜ 0.05).Meanwhile,DHEAS,androstenedione and testosterone of GnRHa treated girls were significantly higher than early menache girls,and DHEAS was higher than normal girls.The size of uterus in treated group was larger than the other two groups.[Conclusion] Early menarche and GnRHa treatment may take negative effect to BMI and glucose metabolism.Androgen was higher in GnRHa treated group.Therefore,suggestion was that BMI,insulin,blood glucose and androgen should be monitored in early menarche girls and girls treated with GnRHa.

Objective To compare the advantages and disadvantages of SNPscan and Sanger sequence which are both used to detect the common deafness gene mutations in non-syndromic hearing loss (NSHL) in Gansu Province.Methods Peripheral blood samples were obtained from Dongxiang, Yugu and Baoan people with moderately severe to profound sensorineural hearing loss in Gansu province to extract genomic DNA.SNPscan was used to detect the 115 mutations in the common pathogenic GJB2 gene, SLC26A4 gene and mtDNA gene.Results We used the SNPscan to screen the mutation of GJB2 gene,mtDNA A1555G and mtDNA C1494T, SLC26A4 gene of sensorinural deafness patients from Gansu Province.The mutation rate of these three genes was 23.18% (35/151), and the mutation rate of Dongxiang, Yugu, Baoan was 21.31% (26/122), 54.54% (6/11), 16.67% (3/18), respectively.Compared with the Sanger sequence, the results were statistically insignificant(P>0.05).The detection rates in the three genes of SNPscan were 11.26% (17/151), 1.32% (2/151) and 0.66% (1/151),respectively , and the detection rates of Sanger sequence were 9.93% (15/151), 1.32% (2/151) and 0.66% (1/151) ,respectively.The results of the two methods were compared.The results were statistically insignificant (P>0.05).Time, cost and flux, SNPscan method is superior to Sanger sequencing.Conclusion Compared with the Sanger sequence, SNPscan is more lighter in workload, less time-consuming, higher-throughput, lower cost, and can get more meaningful mutations and reduce the false negative rates.

BACKGROUND: The evaluation of stereoacuity can provide an unambiguous assessment of haplopia. It is valuable in assessing the diagnosticaccuracy and the therapeutic effect in strabismus, amblyopia and ametropia. OBJECTIVE: This investigation was designed to evaluate the stereopticdevelopment and the occurrence rate of strabismus in 3-6 years old children, and so that to provide objective data for prevention and treatment of strabismus and steropsis. DESIGN: It was a randomized sampling investigation. SETTING: Department of Ophthalmology,Haikou People's Hospital. PARTICIPANTS:Totally 8326 children in 25 urban and suburban kindergartens, with an age of 3-6 years old, were selected randomly in Haikou city from June 2000 to April 2002. METHODS: 8 326 3-6 years old children in Haikou city underwent vision examinations on hyperopia, eye position and the myopia. Those having eyesight lower than 1.0D or abnormal eye position received atropine drops to dilate the pupil of the eye for optometry. ① 4-6 years old children were examined using standard visual chart and logMAR chart (logarithm of the minimum angle of resolution chart), 3 years old children were examined using hroken wheel acuity cards or symbols chart. The results of the exminations were conversed and recorded in form of decimal fraction uniformly. ②The corneal reflection and the cover test procedure were used for the position of gaze. ③ Stereoacuity were examined using the stereoptic visual testing cards (made by the Institute of Biophysics, Chinese Academy of Sciences). Stereoacuity were defined as normal when the test value was ≤ 60 s and were defined as abnormal when it was ≥ 100 s. Each examination was performed by same selected physicians. MAIN OUTCOME MEASURFS: The main outcome measurements included ① results of eye position examination and stereoptic examination in children in all age brackets, ② results of optometry in children with strabismus, ③ The relation between eyesight and stereopsis in participants,④Stereoacuity of children in all age brackets, ⑤and possible causations involve in stereopsis. RESULTS: Practically, 8326 participants receiving examinations of eysight and eye position entered the statistical analysis procedure, while an other 662 children were omitted for their poor expression for the examinations. ① Results of stereoptic examination and eye position examination for participants in all age brackets: 165 (2％) with strabismus, in which 89.7％were first diagnosed; 2016 (27.5) with myopia, whose stereoptic value were ≥ 100 s. ②Results of optometry in children with strabismus: 93.5％ esotropia occurred in children with hyperopia. The rate of hyperopia/myopia was not significantly different between exotropia and vertical strabismus.③ Relation between eyesight and stereoacuity of participants: 75.5％ children have a normal stereoacuity.With a decreasing in eyesight,the stereoacuity decreased (P ＜ 0.05 or P ＜ 0.01). ④ Stereoacuity of the children in all age brackets: With an increasing age, the rate of children with normal stereoacuity increased. ⑤ Possible causations involved in stereopsis: strabismal amblyopia might have the greatest influence on stereopsis. CONCLUSION: ①About 90％ children with strabismus were firstly diagnosed in the investigation. Causations responsible for abnormal stereopsis include ametropia, strabismus, and amblyopia, and so on. ② Concomitant esotropia has close relation with binocular accommodation, while exotropia and vertical strabismus are not linked to refractions. Stereoacuity decreases with a decreasing in eyesight. ③ The results of the investigation support the view that mature stereopsis is achieved early than 4 years old although certain variance of stereoptic development can be observed among individuals.

Objective To explore the relationship between adrenarche and gonadarche.Methods Total 49 idiopathic central precocious puberty(ICPP)girls,whose serum dehydroepiandrosterone sulfate(DHEAS)Z scores for chronological age were higher than+2 s at diagnosis.were enrolled.Physical examinations during pubertal stage were repeated at 3-6 months intervals,and serum DHEAS levels were monitored yearly within an average period of 4.08 years.Of them,16 girls were followed up until more than one year after discontinuation of gonadotropin-releasing hormone analogue(GnRHa)treatment.Results Before GnRHa treatment,these49 girls presented a younger average age at attainment of pubic hair stage2(PH2)and pubic hair stage3(PH3)than normal(8.07 years vs 11.16 years,8.82 years vs 12.40 years respectively).During GnRHa treatment,the intervals between PH2 and PH3,PH3 and pubic hair stage4(PH4),breast stage 2(B2),and PH2 were longer than normal(1.69 years vs 0.83 years,1.64 years vs 0.60 years,and3.62 years vs 0.76 years respectively).The intervals between PH2 and PH3,as well as B2 and PH2 during GnRHa treatment were also longer than that before GnRHa treatment(1.69 years/35 0.88 years,3.62 years vs 1.13 years respectively).The serum DHEAS Z scores decreased during GnRHa treatment,and increased significantly after GnRHa cessation.Conclusion Gonadarche after age of 6-year-old may lead to earlier adrenarehe.GnRHa treatment might slow down the progression of adrenarche and suppress the hypothalamuspituitary-gonadal axis.