Objective To explore the prevalence of poor behaviors and factors among patients with type 2 diabetes in urban areas of Guangzhou.Method Self-designed questionnaire was conducted among 369 patients with type 2 diabetes and factors on poor behaviour were analysed by binany logistic regression.Results Among 369 subjects with type 2 diabetes,80 cases(21.7%)currently smoked,41(11.1%)currently used alcohol and 65(16.9%)were lack of physical activity in leisure time.Multiple logistic regression analysis indicated that female,diabetes-related knowledge and health demands were significantly and negatively associated with smoking,alcohol use and the lack of physical activity; compared with unmarried cases,those who got married,divorced or loosed spouse significantly had the higher risk of smoking,one who got married had the lower risk of using alcohol; the cases with aged and high family income had the lower risk of lack of physical activity in leisure time.Conclusion There were a higher prevalence of poor lifestyle behaviors among these patients with type 2 diabetes,and there was a significant difference of the poor lifestyle behaviors in those with different characteristics,and the cases with good diabetes-related knowledge well encouraged them giving up the poor lifestyle behaviors.

Objective To explore the correlations and status of self-management level and the quality of life of patients undergoing maintenance hemodialysis. Method The self-management behavior rating scale and World Health Organization Quality of Life-brief (WHOQOL-BREF) were used among 59 patients undergoing hemodialysis for the investigation. Results The score by WHOQOL-BREF was (40.56 ± 6.15) and that by the self-management behavior rating scale was (86.99 ± 16.41). The self-management level and the quality of life were positively correlated. Conclusion The measures including enhancing fluid intake, improving patient's compliance, encouraging them to actively participate in physical exercise and social activities can be done to improve their self-management and ultimately improve their quality of life.

Objective To evaluate the effect of oxycodone postconditioning on myocardial ischemia-reperfusion (I/R) injury in rats.Methods Forty pathogen-free healthy adult male Sprague-Dawley rats,weighing 200-300 g,were randomly divided into 4 groups (n =10 each) using a random number table:sham operation group (group S),myocardial I/R group (group Ⅰ),oxycodone postconditioning group (group O),and selective protein kinase C inhibitor chelerythrine group (group CH).Myocardial ischemia was induced by 30 min occlusion of the left anterior descending branch of the coronary artery,followed by 120 min reperfusion.In group S,the left anterior descending branch of the coronary artery was only exposed but not ligated.In group CH,chelerythrine 5 mg/kg was injected intravenously and slowly via the jugular vein before ligation which was performed immediately after administration.In O and CH groups,oxycodone 0.5 mg/kg was injected intravenously and slowly via the jugular vein at 2 min before reperfusion.Arterial blood samples were taken at 120 min of reperfusion to detect the levels of cardiac troponin Ⅰ (cTnI) and creatine kinase-MB (CK-MB) in serum.The hearts were removed after the animals were sacrificed to measure the myocardial infarct size by TTC staining.Results Compared with group S,the levels of cTnI and CK-MB in serum and myocardial infarct size were significantly increased in Ⅰ,O and CH groups (P＜0.05).Compared with group Ⅰ,the levels of cTnI and CK-MB in serum and myocardial infarct size were significantly decreased in O and CH groups (P＜0.05).Compared with group O,the levels of cTnI and CK-MB in serum and myocardial infarct size were significantly increased in group CH (P＜0.05).Conclusion Oxycodone postconditioning can mitigate myocardial I/R injury in rats,and the mechanism is partially related to activation of protein kinase C signaling pathway.

Object A systematic study on the chemical constituents of Citrus changshan huyou Y B Chang was carried out in order to reveal the active components for their further development Methods By repeated silica gel chromatographic separation and spectral analysis the structures were determined Results Six compounds of the flavonoids were obtained and identified They were hesperidin (hysperetin 7 O rutonoside) (Ⅰ); naringenin (Ⅱ); nobiletin (3′, 4′, 5, 6, 7, 8 hexamethoxyflavone) (Ⅲ); tangertin (4′, 5, 6, 7, 8 pentamethoxyflavone) (Ⅳ); 5 hydroxy 3′, 4′, 6, 7, 8 pentamethoxyflavone (Ⅴ); 5 hydroxy 3′, 4′, 6, 7, 8 haxamethoxyflavone (Ⅵ) Conclusion The above six compounds were obtained from this plant for the first time

Objective To evaluate the effect of oxycodone pretreatment on autophagy during renal ischemia-reperfusion (I/R) in rats.Methods Thirty-six SPF healthy adult male Wistar rats,aged 6-9 weeks,weighing 180-220 g,were divided into 3 groups (n=12 each) using a random number table:sham operation group (Sham group),I/R group and oxycodone pretreatment group (Oxy group).The left renal pedicles were clamped with atraumatic microclips for 45 min followed by reperfusion to establish the model of renal I/R injury in I/R and Oxy groups.Oxycodone 0.5 mg/kg was injected via the caudal vein at 15 min before ischemia in group Oxy,and the equal volume of normal saline was given instead in I/R and Sham groups.At 24 h of reperfusion,blood samples were collected from hearts for measurement of serum creatinine (Cr) and blood urea nitrogen (BUN) concentrations.The animals were then sacrificed and left renal tissues were obtained for examination of pathological changes (with a light microscope) and for determination of Bcl-2 and Beclin-1 expression (by immunohistochemistry).Results Compared with Sham group,the concentrations of serum Cr and BUN were significantly increased,and the expression of Bcl-2 and Beclin-1 in renal tissues was up-regulated at 24 h of reperfusion in I/R and Oxy groups (P<0.05).Compared with I/R group,the concentrations of serum Cr and BUN were significantly decreased,the expression of Bcl-2 in renal tissues was up-regulated,and the expression of Beclin-1 in renal tissues was down-regulated at 24 h of reperfusion (P<0.05),and the pathological changes were significantly attenuated in Oxy group.Conclusion Oxycodone pretreatment inhibits autophagy through up-regulating the expression of Bcl-2 and down-regulating the expression of Beclin-1,thus attenuating renal I/R injury in rats.

Objective To investigate the effects of sinomenine on hind limb ischemia?reperfusion ( I∕R) injury and expression of Bcl?2 and Bax in skeletal muscle cells of rats. Methods Fifty?four healthy adult male Wistar rats, aged 6-8 weeks, weighing 180-220 g, were divided into 3 groups ( n=18 each) using a random number table: sham operation group ( group S) , group I∕R and sinomenine group ( group SIN) . The rats were subjected to 4 h of ischemia on the proximal part of the right hind limb using elastic rubber bands followed by reperfusion in I∕R and SIN groups. Sinomenine 60 mg∕kg was injected intraperito?neally at 30 min before reperfusion in group SIN, and the equal volume of normal saline was given instead of sinomenine at 30 min before reperfusion in S and I∕R groups. Immediately after onset of reperfusion and at 4 and 24 h of reperfusion, blood samples were collected from the cardiac apex to measure the concentra?tions of serum lactate dehydrogenase ( LDH) and creatine kinase ( CK) . The animals were sacrificed imme?diately after blood sampling, and the gastrocnemius specimens of the hind limb were immediately removed for determination of the wet to dry weight ratio ( W∕D ratio) and expression of Bcl?2 and Bax in gastrocnemi?us cells ( by immunohistochemistry) and for examination of the pathological changes after haematoxylin and eosin staining. The Bcl?2∕Bax ratio was calculated. Results Compared with group S, the gastrocnemius W∕D ratio and concentrations of serum LDH and CK were significantly increased, the expression of Bcl?2 was significantly down?regulated, the expression of Bax was significantly up?regulated, and the Bcl?2∕Bax
ratio was significantly decreased in I∕R and SIN groups ( P<0?05) . Compared with group I∕R, the gastroc?nemius W∕D ratio and concentrations of serum LDH and CK were significantly decreased, the expression of Bcl?2 was significantly up?regulated, the expression of Bax was significantly down?regulated, and the Bcl?2∕Bax ratio was significantly increased in group SIN ( P<0?05) . The pathological changes of the gastrocne?mius were significantly attenuated in group SIN as compared with group I∕R. Conclusion Sinomenine can attenuate hind limb I∕R injury, and the mechanism may be related to maintenance of the balance between Bcl?2 and Bax and to inhibition of apoptosis in skeletal muscle cells of rats.

Objective To understand the continuous care needs and related services will of the women in puerperium, and analyze the relative factors of the continuity care of those discharged from maternity hospital.Methods A total of 207 delivery women who were discharged from a hospital in Chenghua District Maternal & Child Care Center in Chengdu city from September to December, 2014 were investigated with self-made questionnaires.Results As for newborn babies' continuity care service, what those delivery women care most were newborn babies' common disease prevention and care, and as for their own continuity care service, what they most need were maternal uterine instauration care.Enforcers for continuity of care services were more inclined to obstetricians and pediatricians, and the service forms tended to make telephone calls or do follow-up visits.Time frequency of the follow-up visits was inclined to 7 days once.Delivery women's educational degree (r=-0.216, P＜0.01), place of residence (r=0.208, P＜0.01), whether they would participate in pregnant Women school (r=0.192, P＜0.01), pregnant women school number (r=-0.382, P＜0.01),the newborn's tire(r=0.173, P＜0.05) and maternal type(r=0.161, P＜0.05) were factors that influenced the degree of maternal continued care needs.Conclusions The main factors which influence the continuity care in obstetrics are educational degrees, place of residence and the types of delivery women.As a result, different methods of continuity care service should be established to aim at different groups of people so as to promote the service quality in obstetrics in general to meet the needs of delivery women at all levels.

Objective To compare the effects of oxycodone and morphine on myocardial ischemiareperfusion (I/R) injury in rats.Methods Forty-eight SPF healthy adult male Sprague-Dawley rats,aged 8-10 weeks,weighing 250-300 g,were randomized into 4 groups (n =12 each) using a random number table:sham operation group (S group),myocardial I/R injury group (I/R group),morphine group (M group) and oxycodone group (O group).Myocardial I/R was produced by occlusion of the anterior descending branch of the left coronary artery for 30 min followed by 120 min of reperfusion.In group S,the anterior descending branch of the left coronary artery was only exposed but not ligated.In M and O groups,morphine 1.5 mg/kg and oxvcodone 0.5 mg/kg were injected,respectively,via the internal jugular vein at 5 min before ischemia.At the end of reperfusion,arterial blood samples were collected via cardiac puncture for determination of the serum creatine kinase-MB (CK-MB) and cardiac troponin Ⅰ (cTnI) concentrations.Myocardial infarct size was measured by 2,3,5-triphenyhetrazolium chloride staining.Apoptosis in cardiomyocytes was determined by TUNEL,and apoptosis index (AI) was calculated.Results Compared with S group,serum CK-MB and cTnI concentrations and AI were significantly increased,and myocardial infarct size was enlarged in I/R,M and O groups (P＜0.05).Compared with I/R group,serum CK-MB and cTnI concentrations and AI were significantly decreased,and myocardial infarct size was decreased in M and O groups (P＜0.05).Serum CK-MB and eTnI concentrations and AI were significantly lower,and myocardial infarct size was smaller in O group than in M group (P＜0.05).Conclusion Oxycodone produces better efficacy than morphine in reducing myocardial I/R injury in rats.

Objective To indentify the gene mutation of fibroblast growth factor receptor 3 (FGFR3) gene in a Chinese family with congenital achondroplasia (ACH). Methods The genomic DNA from 2 clinically diagnosed ACH patients and the other 4 members from the same family was prepared for PCR. The products of PCR were purified and then sequenced directly. Results Two patients with ACH in this family showed G-A transition mutation at nucleotide 1138 as heterozygotes. Conclusion The G-A transition mutation at nucleotide 1138 in transmembrane domain of FGFR3 gene seems to be the pathologic cause of this Chinese family with ACH.

Objective To compare the image quality produced by MR high resolution vessel wall imaging (HR?VWI) and ultrasound (US) in evaluating carotid plaque load. Methods This prospective study enrolled 21 patients with carotid plaques undergoing HR?VWI and subsequent 2D US between August 2016 to January 2017 in Hebei General Hospitial. The plaque thickness (PT), lumen area (LA), wall area (WA) and total vessel area (TVA) of the plaques were measured and normalized wall index (NWI) was calculated on both HR?VWI images and US for those plaques with image quality score≥3 and matching between the two methods. The plaque load index was compared by using the independent sample t test or the non?parametric Wilcoxon test, and the correlation between the indexes was based on the Pearson test. Results Forty?five carotid plaques were matched with HR?VWI and US. There was no significant difference in PT, LA, WA, TVA and NWI detected by HR?VWI and ultrasound (P>0.05). The parameters measured by two methods were correlated (r values were 0.83, 0.85, 0.32, 0.83 and 0.59, P<0.05). Conclusion There is a good consistency between HR?VWI and conventional ultrasound in the measurement of carotid plaque load.