OBJECTIVE:To establish HPLC method for the determination of effective components in Huoxue tongluo tablets by HPLC.METHODS:The contents of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Re were simultaneously determined by a HPLC on Agilent C18(150 mm?4.6 mm,5 ?m)column,with mobile phase consisted of acetonitrile-0.05%H3PO4(gradient elution) at a flow rate of 1.0 mL?min-1 and a detection wavelength of 203 nm.RESULTS:The linear ranges of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Re were 0.38～3.80 ?g(r=0.999 6),0.94～9.40 ?g(r= 0.999 5),0.15～1.50 ?g(r=0.999 8),respectively,their average recoveries were 99.23%,97.56%and 107.32%,respectively,and the corresponding RSD were 4.35%,4.94% and 4.77%,respectively.CONCLUSION:The results indicate that the HPLC method is simple,feasible and reproducible,and it is applicable for the quality control of Huoxue tongluo tablets.

OBJECTIVE:To establish a HPLC method for the simultaneously determination of three components in Bikeling nasal spray. METHODS: The column we used was Hypersil ODS and the mobile phase was 0.1%mtriethylamine(adjust pH3.2 with phosphate acid)-acetonitrile (75∶25). The detective wavelength was 256nm and the sample size was 10?L. RESULTS: The linear rangs of ofloxacin, ephedrine hydrochloride and dexamethasone sodium phosphate were 32～320?g?mL-1(r=0.999 9),64～640?g?mL-1(r=1.000 0) and 4～40?g?mL-1(r=1.000 0)respectively, and the average recoveries were 100.05%(RSD=0.32%), 100.17%(RSD=0.48%) and 99.85%(RSD=1.67%),respectively. CONCLUSION: The method was simple and accurate and can be used for the quality control of Bikeling nasal spray