Objective To investigate the effect of treadmill running with different intensities on type Ⅱ collagen (Col2) of knee joint articular cartilages in rats.Methods A total of 48 adult Sprague-Dawley rats were randomly divided into a control (C) group,a low-intensity exercise (L) group,a moderate-intensity exercise (M)group and a high-intensity exercise (H) group,each of 12.Rats in three exercises groups were regularly trained on treadmill at low,moderate,and high intensities respectively.Eight weeks later,all the animals were sacrificed.The right tibial plateau samples were collected to observe collagen fibers under polarizing light microscopy,and the collagen Ⅱ content were examined using immunohistochemistry.The mRNA expression of biglycan (BGN),fibromodulin (FMOD) and Col2 was tested using the quantitative real-time reverse transcription-polymerase chain reaction.Results Compared with group C,collagen fibers in group L and M exhibited almost the same organization,whereas,alteration in organization and shape of collagen fibers was found in group H.Significantly lower content of type Ⅱ collagen was found in group H than that in group C.In comparison with group C,group L had significantly higher gene expression of Col2,whereas group H had significantly higher BGN mRNA expression.Conclusion Low-or moderate-intensity treadmill running appears to have beneficial effect on articular cartilages to maintain its integrity.Highintensity exercises induce lower content and disorder of type Ⅱ collagen in articular cartilages,but the self-healing of cartilage may still exist.

Objective To analyze the effects of losartan and amlodipine on leptin,adiponectin and insulin sensitivity in obese hypertensive patients.Methods 126 patients with obesity related hypertension were selected,and they were randomly divided into losartan group and amlodipine group according to the digital table,63 cases in each group.The losartan group were treated with losartan 50mg/time,1 time/d.The amlodipine group was given amlodipine 50mg/time,1 time/d.The two groups were given the corresponding drugs for 16 weeks.The body mass index (BMI),heart rate,systolic blood pressure (SBP),diastolic blood pressure (DBP),leptin,adiponectin,HOMA-IR and norepinephrine(NE) levels were measured before and after treatment in the two groups.Results After treatment,the BMI of the losartan group decreased significantly,the difference was statistically significant (t =6.660,P ＜ 0.05),but there was no significant difference in heart rate (t =0.180,P ＞0.05).After treatment,the BMI of the amlodipine group decreased significantly (t =3.647,P ＜ 0.05),heart rate increased significantly,the difference was statistically significant (t =3.098,P ＜ 0.05).After treatment,the BMI and heart rate in the losartan group were significantly lower than those in the amlodipine group,and the differences between the two groups were statistically significant (t =3.106,3.256,all P ＜0.05).After treatment,the SBP and DBP in the two groups were significantly decreased,the differences were statistically significant (tlosartan group =9.503,13.095;tamlodipine group =10.254,11.776,all P ＜ 0.05),there were no statistically significant differences between the two groups (t =0.975,0.659,all P ＞ 0.05).After treatment,the level of adiponectin in the losartan group was significantly increased,the leptin level and HOMA-IR score decreased significantly,the differences were statistically significant (t =4.462,2.004,5.572,all P ＜ 0.05),there was no significant difference in the level of NE (t =0.481,P ＞ 0.05).After treatment,NE level of the amlodipine group was significantly higher,the difference was statistically significant (t =6.090,P ＜ 0.05),there were no statistically significant differences in the levels of leptin,adiponectin and HOMA-IR score (t =0.126,0.053,0.448,all P ＞ 0.05).Conclusion Losartan and amlodipine can effectively control hypertension,but losartan can effectively regulate the metabolic disorder of obesity related hypertension patients with obesity alone,therefore,losartan alone has more significant effect compared with amlodipine.