1.Clinical study on root canal filling combined with crown restoration for the treatment of large area defect of deciduous teeth
Yuezhi JIANG ; Wenyun XU ; Hua XU
Chinese Journal of Primary Medicine and Pharmacy 2021;28(4):574-578
Objective:To investigate the clinical efficacy of root canal filling combined with crown restoration in the treatment of large area defect of deciduous teeth.Methods:The clinical data of 160 children patients with large area defect of deciduous teeth who received treatment in Wenling No.4 People's Hospital from January 2017 to January 2018 were retrospectively analyzed. All included children received root canal filling treatment. According to the different postoperative repair methods, these children were divided into two groups. In the observation group ( n = 80), repair with a prefabricated metal crown was performed. In the control group ( n = 80), resin filling repair was given. Gingival health (gingival index) and restoration effect, including secondary caries, gingival condition (congestion or not) and food impaction were compared between the observation and control groups. Tooth condition (marginal tightness, adjacency) and safety (restoration and abrasion) were compared between the two groups after 1 year of follow up. Results:Before restoration, there was no significant difference in gingival index between the observation and control groups [(0.76 ± 0.41) vs. (0.73 ± 0.32), P > 0.05]. After restoration, gingival index in the observation group was significantly lower than that in the control group [(0.86 ± 0.55) vs. (1.35 ± 0.62), t = 5.288, P < 0.001]. In the observation group, no secondary caries or food impaction was observed, and there was one case of gingival bleeding. In the control group, there were eight cases of secondary caries, four cases of good impaction, and nine cases of gingival bleeding. The incidence of adverse reactions in the observation group was significantly lower than that in the control group ( χ2 = 6.826, P < 0.05). One-year follow-up results revealed that 79 cases (98.75%) were qualified in tooth bonding and 78 cases (97.50%) were qualified in tooth occlusion in the observation group, while 70 cases (87.50%) were qualified in tooth bonding and 69 cases (86.25%) were qualified in the occlusion in the control group. There were significant differences in qualified bonding and occlusion between the two groups ( χ2 = 7.907, 6.781, both P < 0.05). The success rate of marginal tightness, the success rate of adjacency, restoration and abrasion in the observation group were higher or superior to those in the control group (98.75% vs. 86.25%, 100.00% vs. 87.50%, 100.00% vs. 85.00%, 98.75% vs. 83.75%) ( χ2 = 9.009, 10.666, 12.973, 11.272, all P < 0.05). Conclusions:Root canal filling combined with crown restoration in the treatment of large area defect of deciduous teeth has a high success rate of qualified bonding and occlusion, less adverse defects, low incidence of food impaction and gingival bleeding, decreases the risk of secondary caries, protects the remaining tooth tissue, maintains normal chewing function, and is conducive to the eruption of permanent teeth.
2.The Plasma Load of Inoculated EIAV Vaccine Strain EIAV_(FDDV) Was Not Boosted by Immune Suppression
Jian MA ; Chenggang JIANG ; Yuezhi LIN ; Liang GUO ; Wei GUO ; Xiangang KONG ; Rongxian SHEN ; Yiming SHAO ; Xiaoyan ZHANG ; Jianhua ZHOU
Progress in Biochemistry and Biophysics 2006;0(11):-
The attenuated Chinese equine infectious anemia virus (EIAV) vaccine is the first lentiviral vaccine that provides solid protective immunities to vaccinated horses. To investigate properties of EIAV vaccine, especially the relationship between its replication and the immunity, viral plasma loads of an EIAV vaccine strain EIAVFDDV in immune suppressed horses were detected. Three horses, which were immunized with EIAVFDDV for 16 months, were treated with dexamethasone for 14 days to suppress their immunities. Reduced immune response in these animals was confirmed by significantly declined lymphocyte proliferation rate detected after 10 days of the drug treatment. The plasma viral loads of EIAVFDDV, which was indicated by the genomic RNA copy numbers, in horses before and after the treatment of dexamethasone were monitored by real time RT-PCR. Results revealed that the viral plasma loads in two of three immune-suppressed horses were kept a steady low level around 103~ 104 copies/ml. The load was increased by 10 folds in the third horse, but was still among the standard levels for EIAVFDDV vaccinated horses. As a positive control, the viral copy number of an asymptomatic carrier of EIAV virulent strain EIAVLiao was jumped nearly 25 000-fold higher after being treated with dexamethasone. The typical clinical symptoms of EIA, characterized by febrile episodes and thrombocytopenia, were also appeared in this horse. These results clearly indicate that it is the unique biological feature of the attenuated EIAV vaccine, but not the immunity, resulted in EIAVFDDV remaining in low levels in the body harmlessly. In addition, the steady low level of viremia and the inability to cause clinical symptoms of EIAVFDDV in immune-suppressed hosts further demonstrated the safety of attenuated Chinese EIAV vaccines. The data provide a new sight for studies on the immunity to lentiviruses.
3.Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application.
Yuezhi LU ; Wenjie ZHANG ; Jie WANG ; Guangzheng YANG ; Shi YIN ; Tingting TANG ; Chunhua YU ; Xinquan JIANG
International Journal of Oral Science 2019;11(2):17-17
Bone defects caused by trauma, tumour resection, infection and congenital deformities, together with articular cartilage defects and cartilage-subchondral bone complex defects caused by trauma and degenerative diseases, remain great challenges for clinicians. Novel strategies utilising cell sheet technology to enhance bone and cartilage regeneration are being developed. The cell sheet technology has shown great clinical potential in regenerative medicine due to its effective preservation of cell-cell connections and extracellular matrix and its scaffold-free nature. This review will first introduce several widely used cell sheet preparation systems, including traditional approaches and recent improvements, as well as their advantages and shortcomings. Recent advances in utilising cell sheet technology to regenerate bone or cartilage defects and bone-cartilage complex defects will be reviewed. The key challenges and future research directions for the application of cell sheet technology in bone and cartilage regeneration will also be discussed.
Bone Regeneration
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Bone and Bones
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Cartilage, Articular
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Regeneration
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Tissue Engineering
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trends
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Tissue Scaffolds