1.Effects of different pH conditions on ffh gene expression in Streptococcus mutans.
Zewen CHEN ; Jing LI ; Kaide LI ; Chuanbin QIU ; Yueyin QIAO ; Jing XUE ; Yuqing LI
West China Journal of Stomatology 2016;34(1):23-26
OBJECTIVEThis research aimed to detect the expression levels of ffh gene in Streptococcus mutans (S. mutans) UA159 under different pH conditions, analyze the effect of pH on the expression of ffh gene in S. mutans, and identify the factors regulating the ffh gene expression.
METHODSSamples of S. mutans were collected at different growth stages (4 h, 18 h) and pH values (pH 4.0-7.0). Fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the relative mRNA expression and trend of the target gene ffh in S. mutans at different growth stages and pH values.
RESULTSqRT-PCR results showed that the ffh gene expression decreased along with pH at 4 h, but the expression increased with decreasing pH at 18 h. Under the same pH conditions, the ffh gene expression was significantly different between 4 h and 18 h (P < 0.05).
CONCLUSIONGrowth stage and pH value influenced the ffh gene expression in S. mutans.
Bacterial Proteins ; Gene Expression ; Gene Expression Regulation, Bacterial ; Hydrogen-Ion Concentration ; Streptococcus mutans
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