OBJECTIVE:To discuss the role of clinical pharmacist on drug use intervention in pediatric ward. METHODS:During Aug.-Dec. in 2014,clinical pharmacists provided whole-course pharmaceutical care for 493 pediatric patients with chronic disease in pediatric ward of our hospital,guided rational drug use,and analyzed and summarized the problems. RESULTS:Clini-cal pharmacists intervened in drug use in 70.99%patients,with success rate of 96%. Among them,top three types of the non-stan-dard medication were:non-standard medication time,incorrect usage and missed medication. CONCLUSIONS:Due to clinical phar-macists’intervention,the problems of drug therapy can be found so as to put forward reasonable suggestions and guidance,reduce the incidence of ADR,and improve the effective rate and success rate.

OBJECTIVE: For the purpose of more accurate and rapid analysis of aconitine in medicine wine, a reversed-phase High Performance Liquid Chromatography (HPLC) method was developed. METHODS: Standard aconitine was added to the blank wine when the wine sample was pretreated. The pretreatment method of samples, the linear range, the precision, the recoveries in the plasma were investigated by using of white wine plasma spiked with standard aconitine. RESULTS: The linear range was 0.45 approximately 9.0 microg x mL(-1), r=0.998 8. The detective limit was 0.45 microg x mL(-1), The intra and inter-day precision of assay for aconitine were less than 3.1% and 4.7% (n=5) in wine respectively. The recoveries of aconitine were more (97.3+/-2.8)% in medicine wine. The HPLC method has been used to investigate the concentration of aconitine in one forensic medicine case. CONCLUSION The HPLC method of quantitative analysis aconitine is rapid and sensitive. It is only in the 2.0 h that determination of aconitine in the medicine wine.
Aconitine/analysis* ; Chromatography, High Pressure Liquid/methods* ; Forensic Medicine ; Humans ; Male ; Middle Aged ; Quality Control ; Sensitivity and Specificity ; Time Factors ; Wine/analysis*

OBJECTIVETo evaluate the effect of Helicobacter pylor (Hp) eradication in children with acute idiopathic thrombocytopenic purpura (ITP).

METHODSNinety-three children with acute ITP and Hp infection were divided into two groups and treated with prednisone and Hp eradication (group A, 51 cases) or with prednisone without Hp eradication (group B, 42 cases).

RESULTSThe Hp eradication rate was 94.1% in group A. No difference was found in the therapeutic effects on IPT between the two groups, but the recurrence rate in one year in group A was significantly lower than that in group B.

CONCLUSIONNHp eradication does not obviously enhance the therapeutic effect on childhood acute ITP, but can decrease the relapse rate in one year. HP eradication therapy is recommended in children with acute ITP and Hp infection.

Adolescent ; Anti-Bacterial Agents ; therapeutic use ; Child ; Child, Preschool ; Drug Therapy, Combination ; Female ; Helicobacter Infections ; drug therapy ; Helicobacter pylori ; drug effects ; immunology ; Humans ; Male ; Prednisone ; therapeutic use ; Purpura, Thrombocytopenic, Idiopathic ; immunology ; microbiology

To explore the differential expression of serum miRNAs in patients of advanced non- small cell lung cancer (NSCLC) treated by gifitinib before and after acquiring drug resistance.
 Methods: A total of 4 patients with advanced NSCLC from Affiliated Hospital of Yueyang Vocational Technical College, who acquired drug resistance during gefitinib therapy from June 2013 to June 2015, were enrolled. Serum samples were collected before treatment and after acquiring drug resistance. MicroRNA (miRNA) microarray was used to assess the levels and compositions of miRNAs in serum. Real-time RT-PCR was used to validate the results of miRNAs with significant differences in expression. The candidate miRNAs inhibitors and mimics were transfected into lung cancer cells by liposome, and the sensitivity of lung cancer cells to gifitinib was detected.
 Results: The miRNA microarray showed that there were significantly differential expression of miRNAs in serum of NSCLC patients after acquiring drug resistance, and 24 miRNAs were changed in more than 2-fold. Among them, 19 miRNAs were up-regulated and 5 miRNAs were down- regulated (both P<0.05). Especially, the expression of miR-21 in serum of NSCLC patients after obtaining resistance was up-regulated more than 10-fold compared with that before treatment. The results of RT-PCR was consistent with the results of miRNA microarray. The up-regulation of miR-21 in lung cancer cells could elevate the half maximal inhibition concentration (IC50) of gefitinib, and the down-regulation of miR-21 in lung cancer cells could reduce the IC50 of gefitinib (both P<0.05).
 Conclusion: There is differential expression of miRNAs in serum of NSCLC patients before treatment and after acquiring drug resistance during gefitinib therapy. The up-regulation of miR-21 may be involved in regulating the acquiring drug resistance of gefitinib.
Antineoplastic Agents ; pharmacology ; therapeutic use ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; Cell Line, Tumor ; Drug Resistance, Neoplasm ; drug effects ; genetics ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Lung Neoplasms ; drug therapy ; MicroRNAs ; blood ; genetics

OBJECTIVETo explore the mechanism of Shengji Huayu Recipe (SJHYR) and its 3 disassembled formulae in accelerating wound healing toward skin restoration.

METHODSUsing in vitro cultured fibroblasts from granulation tissue of wound and compared with the normal skin fibroblast of suckling rat, who were treated separately by drug serum containing high and low dose SJHYR and its disassembled prescriptions (Shengji formula and Huayu formula). The type I and III collagen contents in the fibroblasts were determined with immunocytochemical ABC method.

RESULTSShengji formula could increase the levels of type I and III collagens in fibroblasts, which was significantly higher than those in model cells and control (P < 0.01). Huayu formula lowered them to the levels below those in model cells (P < 0.01), while in comparing the levels in the cells treated by high or low dose SJHYR with those in control, no significant difference was shown(P > 0.05).

CONCLUSIONSJHYR might accelerate wound healing toward skin restoration through regulating the ratio of collagen type I and III, to adjust their metabolism.

Animals ; Animals, Newborn ; Cells, Cultured ; Collagen Type I ; biosynthesis ; Collagen Type III ; biosynthesis ; Drugs, Chinese Herbal ; pharmacology ; Fibroblasts ; metabolism ; pathology ; Male ; Phytotherapy ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Wound Healing ; drug effects ; Wounds and Injuries ; pathology

To study the interaction of traditional Chinese medicines and Western drugs in the interest of the interaction of Chinese and Western drugs viewing from their own characteristics, pharmacokinetics and pharmacodynamics to provide a scientific basis for rational clinical utilization of drugs. Pharmacological mutual coordination and/or restriction existed between the two kinds of drugs. When they were used in combination, it would increase or decrease adverse reactions, or even toxic reaction. When traditional Chinese drugs were used in combined with Western drugs, it is ought to know the nature and pharmacological effect of various chemical constituents in the compound for the best combination, try to avoid adverse drug interactions for the sake of reaching the goal of high effectiveness, safety and harmlessness.
Drug Incompatibility ; Drug Interactions ; Drugs, Chinese Herbal ; Medicine, Chinese Traditional

Objective To determine the serum levels of tumor necrosis factor (TNF)-α,interleukin (IL)-17,IL-22 and IL-17F in patients with palmoplantar pustulosis (PP),and to estimate their relationship with disease activity in PP.Methods Venous blood samples were collected from 30 patients with PP at both active stage and stationary stage and from 20 healthy human controls.Enzyme-linked immunosorbent assay (ELISA) was conducted to determine the serum levels of TNF-α,IL-17,IL-22 and IL-17F.The paired Wilcoxon signed rank test was carried out to compare the serum levels of cytokines between patients at active stage and at stationary stage,and the Mann-Whitney U test to compare those among different groups.Results The median serum levels of TNF-α,IL-17 and IL-22 in patients with PP at active stage were 186.35 (range,113.48-412.69) ng/L,420.45 (range,278.55-748.73) ng/L and 106.48 (range,69.13-251.86) ng/L respectively,significantly higher than those at stationary stage (42.52(18.83-95.37) ng/L,48.11 (36.43-80.04) ng/L,20.32 (10.55-48.75) ng/L,respectively,all P ＜ 0.05) and those in the controls (24.30 (12.0-61.56) ng/L,10.49 (6.24-24.44) ng/L,2.58 (1.41-5.78) ng/L,respectively,all P ＜ 0.05).Moreover,the patients at stationary stage showed a significant elevation in serum levels of TNF-α,IL-17 and IL-22 compared with the controls (u =2.71,3.53,2.18,respectively,all P ＜ 0.05).No statistical difference was noted in the serum level of IL-17F among the patients at different stages and controls (P ＞ 0.05).Conclusion The circulating levels of TNF-α,IL-17 and IL-22 were associated with disease activity in PP,hinting that they may be involved in the development of PP.

ObjectiveTo investigate the infection situation of AIDS， syphilis and hepatitis B among pregnant women in Yueyang City， to provide formulated treatment and intervention measures to reduce mother-to-child transmission， and to improve the quality of birth population. MethodsA total of 24 546 pregnant women who agreed to take the tests of AIDS， syphilis and hepatitis B from January 2018 to December 2021 in our hospital were retrospectively analyzed. ResultsThe positive rate of human immunodeficiency virus antibody （anti-HIV） was 0.045%； positive rates of syphilis antibody （anti-TP） and HBsAg were 4.64% and 0.64%， respectively. The positive rates of anti-HIV， anti-TP and HBsAg varied from 2018 to 2021， but the differences were not statistically significant （P>0.05）. The positive rate of HBsAg increased gradually with the increase of maternal age， and the difference was statistically significant （P<0.01）. There were 16 mixed infections among 1 309 positive cases， 15 of which were hepatitis B and syphilis mixed infections. ConclusionThe infection of AIDS， syphilis and hepatitis B in pregnant women in Yueyang City is relatively high. The screening of AIDS， syphilis and hepatitis B before pregnancy is helpful to prevent and reduce the risk of mother-to-child transmission of infectious diseases in time， which is of great significance to eugenic and eugenic education.

OBJECTIVETo study the major risk factors and characteristics of Schistosoma japonicum in the newly identified areas around the Dong Ting Lake.

METHODSData was collected regarding the distribution of snails, prevalence of infection as well as the source of transmission in the newly identified areas in Hunan province from 1979 to 2003.

RESULTSIn all the newly identified sites, Schistosomiasis japonica patients appeared to be the first-seen evidence, some even under acute phase. The highest infection rate among residents had reached 87.1% in the newly identified villages. Most of the new endemic areas concentrated in the ditch and beach areas that close to cities and towns.

CONCLUSIONSEvidence showed that snails had been transferred through boats and fishing nets to be disseminated. Data also revealed that the sources of transmission would include those local infected residents who had been infected in the old endemic areas and the newly imported domestic animals with infection.

Adolescent ; Adult ; Animals ; Cattle ; Child ; Child, Preschool ; China ; epidemiology ; Disease Outbreaks ; Disease Reservoirs ; Female ; Health Education ; Humans ; Male ; Middle Aged ; Prevalence ; Schistosomiasis japonica ; epidemiology ; prevention & control ; Snails ; parasitology

To obtain higher potency and specificity, a series of 7-alkoxy analogues of illudalic acid was synthesized on the base of structure-activity relationship (SAR). All of these compounds exhibited submicromolar inhibition of the enzyme when tested against human leukocyte common antigen-related phosphatase (LAR) (for example, for 15e, IC50 = 180 nmol x L(-1)). They represent the most potent small-molecule inhibitors of LAR so far. These analogues also display excellent selectivity for LAR over other protein tyrosine phosphatases (PTPs) except for the highly homologous PTPsigma. The compound 15f is of 120-fold selectivity for LAR versus PTP-1B inhibition. The development of potent enzyme-specific inhibitors is so important that they may serve both as tools to study the role of LAR and as therapeutic agents for treatment of type II diabetes.