Solanum Nigrum is a kind of dicotyledon solanaceae annual herb, and alkaloids are its main bioactive constituents, with good antitumor activity. The current extraction methods for alkaloids include solvent extraction, ultrasonic extraction, microwave extraction, supercritical fluid extraction, and column chromatography etc, while the detection methods include acid dye colorimetry, thin layer scanning, high performance liquid chromatography, reversed-phase high performance liquid chromatography, and ultra-high performance liquid chromatography etc. This article reviewed the research progress in extraction and detection methods of alkaloid from Solanum Nigrum in recent years, so as to provide references for further research and development of alkaloid from Solanum Nigrum.

Cerebral palsy greatly affects walking function of the child patients, showing low-effective walking gait. Orthosis is used to change function features or structures of nerve, muscle and skeletal system. Ankle-foot orthosis is commonly used, which could provide appropriate movement control on sagittal, coronal and horizontal planes, maintain joint line of force arrangement, promote functions and improve gait. However, ankle-foot orthosis exhibits some limitations, such as limited range of joint motion, limited muscle function, and infection due to local skin compression. Therefore, clinical doctors and researches are looking for improvements over the past years, for example, to supplement some treatment or protective function based on current orthosis, so as to obtain better clinical outcomes. Appropriate and individualized orthosis is development direction of orthosis creation and design.

Objective To investigate the expression of PTTG and VEGF-C in laryngeal carcinoma and the effect of angiogene-sis .Methods Immunohistochemistry was used to detect the expression of PTTG 、VEGF-C and LMVD in 60 cases of laryngeal car-cinoma and 32 cases of para-carcinoma tissue .D2 40 positive products was used to locate lymphatic endothelial cell cytoplasm and cell membrane ,and count lymphatic microvessel density (LMVD) .Results The expression of PTTG and VEGF-C in laryngeal car-cinoma was significantly higher than that in para-carcinoma tissue(P<0 .05) .In laryngeal carcinoma ,the expression of PTTG and VEGF-C were associated with differentiation ,lymph node metastasis and clinical stage(P<0 .05) ,but independent of clinical classi-fication ,smoking history ,tumor′size ,sex and gender(P>0 .05) .The expression of LMVD in laryngeal carcinoma was significantly higher than that in para-carcinoma tissue(P<0 .05) .The expression of LMVD was associated with lymph node metastasis (P<0 .05) ,but independent of differentiation ,clinical classification ,clinical stage smoking history ,tumor′size ,sex and gender ( P>0 .05) .A significantly positive relation was found between PTTG and VEGF-C(P<0 .05) .And there were positive relation between PTTG and LMVD、VEGF-C and LMVD(P<0 .05) .Conclusion PTTG and VEGF-C might play an important role in the carcino-genesis and development of laryngeal carcinoma .PTTG and VEGF-C could be a prognostic factor of colorectal cancer and a new tar-get of gene therapy .

Objective To investigate the effects of self-efficacy-oriented nursing intervention on postoperative quality of life of lung tumor patients. Methods Ninety patients with lung tumors undergoing chest surgery in our hospital from March 2010 to March 2012 , involved in the study. The nursing data were retrospectively analyzed for investigation of the nursing strategies. The patients were randomized into two group with random digits table in equal number: The control group received routine nursing and the intervention group with self-efficacy-oriented nursing intervention beside routine nursing care. 3 months after nursing intervention , the two groups were compared by Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS) in terms of quality of life and psychological state. Results The scores of the intervention group by SDS and SAS were both significantly higher than those of the control group (P < 0.05). The scores of the intervention group on self efficacy and quality of life were significantly higher than those of the control (P < 0.05). Conclusion The self-efficacy-oriented nursing intervention applied in the postoperative nursing care to lung cancer patients can significantly improve the postoperative symptoms and psychological status. It can also improve their self efficacy and then enhance their quality of life.

AIM:To evaluate the antitumor effect of caffeic acid Ge on U14 tumor bearing mice.METHODS:The tumor inhibitory ratios of caffeic acid Ge on the growth of U14 in mice was observed.Apoptosis morphological transformation of U14 cells induced by caffeic acid Ge was detected by electronic scan microscope and MG-P staining.Alteration of cell cycle was analyzed by flow cytometry.Apoptosis-related protein levels of Bax and Bcl-2 were determined by immunity histochemistry technology.MTT assay was applied to study the antitumor activities of caffeic acid Ge in U14 cell lines in vitro.RESULTS:Tumor inhibitory rates in caffeic-acid Ge groups were 38.50%,47.17% and 64.02%(from low dose to high dose)(P

Objective To analyze the quality nursing measures for upper digestion tract hemorrhage treated by gastroscopy.Methods A total of 100 patients with upper gastrointestinal bleeding admitted in our hospital were randomly divided into control group (n =50) and study group (n =50).Patients of two groups were treated with endoscopy before surgery,the control group was given routine nursing intervention,and the study group was given quality care intervention on the basis of the control group.The changes of indicators (DBP,SBP,HR) in the two groups during the perioperative period (preoperative,intraoperative and postoperative),the changes of anxiety before and after intervention,and the quality of life at 1 month after operation were compared.Results The intraoperative and postoperative DBP,SBP and HR of the study group were lower than those of the control group (P ＜ 0.05).The incidence of severe anxiety in the study group was lower than the control group (10.00％ vs.38.00％,P ＜ 0.05).The proportion of patients without anxiety was higher than that of the control group (60.00％ vs.30.00％,P ＜ 0.05).The scores of sleep,energy,emotion,physical activity,pain and social life were lower in the study group than that in the control group (P ＜ 0.05).Conclusion High quality care measures for upper gastrointestinal bleeding can stabilize the physiological indicators of patients,but also ease anxiety,and improve the quality of life.

Objective To explore the effect of self-efficacy theory on nursing of patients with lung cancer.Methods Totals of 80 patients with lung cancer were randomly divided into study group and control group,control group received routine treatment and nursing,while study group received self-efficacy intervention measures.Between the two groups,patients' self-efficacy and quality of life (WHOQOL-100) score were compared.Results The score of self-efficacy in study group and control group respectively was (37.05 ± 4.23) and (31.12 ±5.21).And the quality of life (WHOQOL-100) score in study group and control group was (77.14 ±4.25) and (62.03 ± 4.52),respectively.Patients' self-efficacy and quality of life scores of two groups were significantly increased (t =5.607,15.403,respectively; P ＜ 0.01).Conclusions Self-efficacy theory has a guiding meaning for pain care of cancer patients,which can improve the quality of life of patients.

Objective To explore structured psychological intervention in improving lung cancer patients’ preoperative self-efficacy.Methods Totals of 60 patients with lung cancer were randomly divided into the study group and the control group .The control group was given conventional nursing care .The study group was given structured psychological nursing intervention , including psychological support , health education , stress treatment and coping technique .Before and after 5 days of the intervention , patients ’ self-efficacy was compared.Results The General Self Efficacy Scale ( GSES) scores before and 5 days after intervention were (26.25 ±4.05) and (36.23 ±4.54) in the study group, and (26.18 ±6.21) and (30.05 ±5.05) in the control group .And the structured psychological intervention can significantly improve the preoperative self -efficacy level of patients with lung cancer (t=4.98,P<0.05).Conclusions The structured psychological intervention can enhance preoperative patients ’ self-efficacy, and can effectively help patients cope with disease and surgery.

Objective To analyze the quality nursing measures for upper digestion tract hemorrhage treated by gastroscopy.Methods A total of 100 patients with upper gastrointestinal bleeding admitted in our hospital were randomly divided into control group (n =50) and study group (n =50).Patients of two groups were treated with endoscopy before surgery,the control group was given routine nursing intervention,and the study group was given quality care intervention on the basis of the control group.The changes of indicators (DBP,SBP,HR) in the two groups during the perioperative period (preoperative,intraoperative and postoperative),the changes of anxiety before and after intervention,and the quality of life at 1 month after operation were compared.Results The intraoperative and postoperative DBP,SBP and HR of the study group were lower than those of the control group (P ＜ 0.05).The incidence of severe anxiety in the study group was lower than the control group (10.00％ vs.38.00％,P ＜ 0.05).The proportion of patients without anxiety was higher than that of the control group (60.00％ vs.30.00％,P ＜ 0.05).The scores of sleep,energy,emotion,physical activity,pain and social life were lower in the study group than that in the control group (P ＜ 0.05).Conclusion High quality care measures for upper gastrointestinal bleeding can stabilize the physiological indicators of patients,but also ease anxiety,and improve the quality of life.