Objective To explore the effects of nicorandil on cardiac function and blood glucose in patients with chronic heart failure(CHF). Methods 64 patients with CHF were randomly divided into control group and treatment group. Both 2 groups received conventionaltherapy for heart failure. And the treatment group was treated with nicorandil additionally (5 mg once, 3 times a day for 12 weeks). 6-minutewalk text (6MWT), plasma N-terminal-pro brain natriuretic peptide (NT-proBNP), fasting plasma glucose (FPG), postprandial 2 hours plasmaglucose (P2hPG) and glycated hemoglobin A1c (GHbA1c) were measured before and after treatment. Results After the treatment, the6-minute walking distance and the plasma NT-proBNP levels of both groups were improved significantly (P<0.01), especially in treatmentgroup (P<0.05); the level of FPG, P2hPG and GHbA1c were not significantly different between 2 groups. Conclusion Nicorandil can effectivelyimprove the exercise tolerance of the patients with CHF, reduce the Plasma NT-proBNP level, but it has no effect on the level of bloodglucose.

Solanum Nigrum is a kind of dicotyledon solanaceae annual herb, and alkaloids are its main bioactive constituents, with good antitumor activity. The current extraction methods for alkaloids include solvent extraction, ultrasonic extraction, microwave extraction, supercritical fluid extraction, and column chromatography etc, while the detection methods include acid dye colorimetry, thin layer scanning, high performance liquid chromatography, reversed-phase high performance liquid chromatography, and ultra-high performance liquid chromatography etc. This article reviewed the research progress in extraction and detection methods of alkaloid from Solanum Nigrum in recent years, so as to provide references for further research and development of alkaloid from Solanum Nigrum.

Objective To investigate the differences in some measured paremeters on spino-pelvic sagittal X-ray films in middle-aged and elderly patients between lumbar spondylolysis and isthmic spondylolisthesis,and to explore the predictors that the spondylolysis at lumbar 5 may develope into spondylolisthesis in order to provide imaging details for the clinical treatment of teenage patients with lumbar spondylolysis.Methods Imaging data of 60 patients with spondylolysis at lumbar 5 and other 60 with isthmic spondylolisthesis in middle or elder age in our hospital were analyzed retrospectively,and a control group with 60 normal volunteers were recruited in this study.Some parameters including the angles of pelvic incidence (PI),pelvic tilt (PT),sacral slope (SS),lumbar-sacral angle (LSA)and lumbar lordosis (LL),and the sagittal vertical axis (SVA)were measured in sagittal radiographs of the spine and pelvis. Differences in sagittal parameters among 3 groups were analyzed by one-way variance analysis and SNK-q test.Results The PI,PT, SS and LL were higher,and LSA was lower in patients with isthmic spondylolisthesis than those in the control group and lumbar spondylolysis one (P<0.05).However,there was no significant difference in SVA (P>0.05).No significant differences in all spino-pelvic sagittal parameters were found between lumbar spondylolysis group and the control (P>0.05).Conclusion Such spino-pelvic sagit-tal parameters as PI,PT,SS,LL and LSA on X-ray film can be regarded as predictors that lumbar 5 spondylolysis may develop into spondylolisthesis and may provide imaging reference for the clinical treatment of teen-age patients with lumbar spondylolysis.

Objective To evaluate the influence of metabolic syndrome(MS) on the left ventricular hypertrophy in non-diabetic hypertensive patients.Methods A total of 321 essential hypertensives were included in which 134 patients were diagnosed as MS(EH+MS),187 patients with hypertension alone(EH) as controls.Left ventricular mass index(LVMI) and relative wall thickness(RWT) were determined by echocardiography.Results Compared with hypertension alone(EH) patients,hypertensive patients with MS(EH?MS) were shown to have greater end-diastolic left ventricular internal dimension(LVEDd),end-diastolic interventricular septum thickness (IVSD),end-diastolic posterior wall thickness(LVPWd) and left ventricular mass indexed(LVMI)(P0.05).Left ventricular mass index(LVMI) was strongly related with waist circumstance(r=0.692,P

BACKGROUND: There are some limitations in induced differentiation of bone-marrow-derived mesenchymal stem cells (hBMSCs) and transplantation of nucleus pulposus cells (NPCs). Inhibition of autologous NPCs degeneration is expected to become an effective way for treatment of disc degeneration in the future. OBJECTIVE: To establish a layered coculture model of hBMSCs and NPCs in vitro, and to observe the effect of hBMSCs on the differentiation of NPCs. METHODS: The fourth passage of hBMSCs was divided into 2 groups. NPCs in the new-style layer culture group were incubated in Transwell cabin without arm, 0.4-μm membrane well; the proportion to BMSCs was 1: 1. NPCs in the traditional culture group were incubated in Transwell cabin with arm. NPC culture group was set. Each group was incubated for 7 days. Collagen Ⅱ was detected by immunohistochemical method. Aggrecan expression was detected by [~(35)S ]-sulfate uptake. RESULTS AND CONCLUSION: Compared with NPC culture group, collagen Ⅱ and aggrecan expression of NPCs were upregulated most evidently in new-style culture and traditional culture groups (P < 0.05, P < 0.01). The increased range of new-style culture group was greater than the traditional culture group (P < 0.05). These results suggested that coculture of BMSCs and NPCs can significantly increase NPC proliferation and specific substance expression. The surrounding of new-style culture surpassed the traditional culture.

BACKGROUND: Removable partial denture is the main way of repairing dentition defects, and its casting materials mainly include pure titanium, cobalt-chromium al oy and Vital um 2000. OBJECTIVE: To analyze the biocompatibility of removable partial dentures made of different materials in repair of dentition defects. METHODS: Total y 120 patients with dentition defects, aged 24-68 years old, were enrol ed and underwent repair with removable partial dentures. Among these patients, 40 patients were treated with cobalt-chromium al oy-casted removable partial dentures, 40 patients with pure titanium-casted removable partial dentures, and 40 patients with Vital ium 2000 casted removable partial dentures. Al patients were fol owed up for 2 years. The chewing efficiency, coagulation, thrombosis, alveolar bone loss, patient satisfaction, and the incidence of adverse reactions, such as abutments periodontal disease and secondary caries were compared between these three groups. RESULTS AND CONCLUSION: (1) Chewing efficiency and patient satisfaction rate: Vital um 2000 group > titanium group > cobalt-chromium al oy group (P < 0.05). (2) Coagulation, thrombosis, the incidence of alveolar bone loss: Vital um 2000 group < titanium material group < cobalt-chromium al oy group (al P < 0.05). (3) Incidence of adverse reactions: Vital um 2000 group < titanium group

Cerebral palsy greatly affects walking function of the child patients, showing low-effective walking gait. Orthosis is used to change function features or structures of nerve, muscle and skeletal system. Ankle-foot orthosis is commonly used, which could provide appropriate movement control on sagittal, coronal and horizontal planes, maintain joint line of force arrangement, promote functions and improve gait. However, ankle-foot orthosis exhibits some limitations, such as limited range of joint motion, limited muscle function, and infection due to local skin compression. Therefore, clinical doctors and researches are looking for improvements over the past years, for example, to supplement some treatment or protective function based on current orthosis, so as to obtain better clinical outcomes. Appropriate and individualized orthosis is development direction of orthosis creation and design.

ObjectiveTo observe the therapeutic efficacy of L-carnitine on patients with microvascular angina (MVA).Methods48 MVA patients were divided randomly into the trail group and control group with 24 cases in each group. The patients of the trail group were treated with L-carnitine vein dropping, those of the control group took isosorbide-mononitrate orally. All treatment lasted for 12 weeks.ResultsAfter the treatment, attack times of MVA and consumption of nitroglycerin of the patients in two groups decreased obviously ( P<0.01). The time for inducing angina, the time for 0.1 mV ST segment depression, the total exercise time and ∑ST depression by exercise were improved obviously ( P<0.05). The serum total cholesterol, triglyceride and low density lipoprotein cholesterol decreased by 20.3%, 32.7% and 16.6% respectively, and high density lipoprotein cholesterol levels increased by 34.5% in the trail group ( P<0.01).ConclusionL-carnitine has affirmative effect on MVA, same as isosorbide-mononitrate.

BACKGROUND:The laser applied in the clinical oral medicine is mainly for early diagnosis of caries, removal of carious tissue, pulpotomy for pulp bleeding, dentin hypersensitivity treatment, disinfection of infected root canals, and periodontitis treatment. OBJECTIVE:To explore the changes of basic fibroblast growth factor in inflammatory pulp after laser treatment. METHODS:Five healthy adult beagle dogs were enroled, and six teeth were randomly selected from each dog. Then, these teeth were randomly divided into three groups, 10 teeth in a group, and they were treated with laser irradiation, saline and gentamicin irrigation respectively. At days 1, 2, 3 after operation, exudates from the pulp were colected for detection of basic fibroblast growth factor expression. Meanwhile, the dental pulp was detected by hematoxylin-eosin staining and immunohistochemistry method. RESULTS AND CONCLUSION:In the al three groups, the expression of basic fibroblast growth factor at day 3 was significantly higher than that at days 1 and 2, and there were no differences between days 1 and 2. Moreover, these three groups also showed no significant differences. Under hematoxylin-eosin and immunohistochemical staining, blood vessels in the pulp cavity were in good condition and the pulp arranged tightly. These findings suggest that the laser is safe, convenient and effective for oral clinical application, and has no injury to the inflammatory pulp.

Objective Using ROC curve to determine the best cut-off point of serum HE-4 in the diagnosis of ovarian cancer and provide important value to diagnosis early ovarian cancer.Methods The levels of serum HE-4 in 68 ovarian cancer pa-tients,42 ovarian benign tumor patients and 30 healthy female were detected by electrochemistry irradiance method.The ROC curve was drawn and the cut-off point of HE-4 was determined by statistical software.Results The levels of serum HE-4 were all non-normal distribution in the groups of ovarian cancer,ovarian benign tumor and healthy controls.Whats more,there was no significant difference between ovarian benign tumor group and normal control group.And compared with benign ovarian tumors and normal control group,the level of HE-4 in ovarian cancers was significantly increased (P <0.01).It would be best for diagnosis when the level of serum HE-4 was 108pmol/L in ovarian cancer.Youden’s index showed the maximum (0.713)and the sensitivity and specificity of diagnosing were 77.9% and 93.1% respectively.The positive predictive value was 91.4% and negative predictive value was 91.4%.At the same time,the positive likelihood ratio was 11.6 and the negative likelihood ratio was 0.2,odds ratio reached to 47.3.Conclusion The detection of HE-4 is an ideal mark for diagnosing and excluding ovarian cancer.Selecting 108 pmol/L as ovarian cancer diagnosis point is relative appro-priate.