The main nutrient compositions of dry Porphyra haitanensis cultivated in Lianjiang,Fujian Province were analyzed: the total amino acids content was 35.48%. The scores of amino acids were 71 for Ile and 80 for Leu. Crude fatty acids was as low as 0. 5%,in which EPA took 24.8%. Minerals in Porphyra haitanensis were abundant. The content of Ca、Fe、 Zn、Mg were 3. 63、0. 56、0. 095 and 3. 29mg?g～-1. The results indicated that Porphyra haitanensis is a kind of health food with high nutrition.

Objective To assess the association of 5-hydroxytryptamine 2A (5-HT2A) receptor gene polymorphism with schizophrenia by Meta analysis.Methods Literatures on the relationship between 5-HT2A receptor gene polymorphism T102C and the Chinese schizophrenia in EMBASE,PubMed,CBM,CNKI and Wanfang Med Online were taken as research objects.Screening the relevant data according to the included and excluded standard,then,Software of RevMan 5.0 was employed to do Meta analysis.Meanwhile,we inspect the literatures publication bias.Results Twelve literatures were included,collecting 4 728 individuals,enrolling 2 135 for the schizophrenia group and 2 593 for the control group.The research conforms to Hardy Weinberg equilibrium (all P＞0.05).The Meta analysis results showed that there was no significant association between 5-HT2A receptor gene polymorphism and schizophrenia including the allele model,recessive model and codominant model genotypes (all P＞ 0.05).The results of Dominant genetic model I2 =45％ with moderate heterogeneity,but we used subgroup analysis to remove the author Ni's data,there were significant difference analysis (I2=9％,Z=2.01,P＜0.05).Conclusions 5-HT2A receptor gene T102C site C allele is recessive genes,CC may be a healing factor,there needs evidence-based study provides further evidence.

BACKGROUND: Cardiac stem cells can differentiate into cardiomyocytes in vitro under induction of pilose antlerpolypeptides, which provides a new therapeutic idea for myocardial injury.OBJECTIVE: To explore the effect of pilose antler polypeptides on the apoptosis rate and membrane stability ofmitochondria in cardiac stem cells.METHODS: We chose healthy male Wistar rats born 2 days to extract cardiac stem cells. The culture dish was used asthe experimental unit, and extracted cells were divided into the following four groups (n=12). Blank control group: Thesame amount of buffer was added for induction; 5-azacytidine group: induced with 5-azacytidine (3 μmol/L); pilose antlerpolypeptides group: induced with pilose antler polypeptides (800 mg/L); combined group: induced with pilose antlerpolypeptides (800 mg/L) and 5-azacytidine (3 μmol/L). After 48 hours induction, apoptosis rate of cardiac stem cells ineach group was detected with flow cytometry. The membrane potential of mitochondria in cardiac stem cells wasdetected with immunofluorescence. The expression level of Nkx 2.5, GATA4, ATF-2, and MEF-2C in cardiac stem cellswas detected using western blot assay.RESULTS AND CONCLUSION: There were small, round and bright cells after 2 weeks culture, and cell colonies ofcardiac stem cells formed. The apoptosis rate of cardiac stem cells in the 5-azacytidine group increased significantly (P <0.05) and its membrane potential decreased significantly compared with the blank control group (P < 0.05). Theapoptosis rate of cardiac stem cells in the pilose antler polypeptides group and combined group decreased significantlycompared with the 5-azacytidine group (P < 0.05, P < 0.01), and their membrane potential increased significantly (P <0.05, P < 0.01). The results of western blot showed that the expression level of Nkx2.5 increased significantly in thepilose antler polypeptides group and combined group compared with the 5-azacytidine group (P < 0.05), while theexpression levels of GATA4 and ATF-2 in each group were low and there were no significant differences among groups(P > 0.05). The expression level of MEF-2C in each group was at a middle level, and there were no significantdifferences among groups (P > 0.05). To conclude, our experimental findings indicate that pilose antler polypeptidescould decrease the apoptosis rate and improve membrane stability of mitochondria in cardiac stem cells. Themechanism may be related to the increased expression of Nkx 2.5, but whether the mechanism is related to GATA4,ATF-2 and MEF-2C needs to be further studied.

ObjectiveTo investigate the relationship between the social disability and emotion disorder of the patients with irritable bowel syndrome (IBS). Methods168 patients with IBS were assessed with Social Disability Screening Schedule (SDSS), Hamilton Anxiety Rating Scale, and Hamilton Depression Rating Scale. The patients with SDSS≥2 were divided into 2 groups, and accepted the medical routine therapy and explanatory psychotherapy for 2 months, while the patients in the research group (n=35) received the antidepressive. Then they were assessed with SDSS again. ResultsThe incidence of social disability in IBS patients was 40.5%, and increased in those who combined with anxiety and/or depression. The SDSS score of the research group was lower than that of the control group after the treatment. ConclusionThe social disability can be found in the patients of IBS, especially in those with emotion disorder, and can be improved by the antidepressive.

Objective To further clarify the pathogenesis of different types of Kaposi′s sarcoma (KS) by measuring the protein expressions of caspase-3 and E-cad in tumor tissues of Xinjiang Uygur patients with acqured immunodeficiency syndrome (AIDS)-Kaposi′s sarcoma (KS) and classical KS.Methods From July 2011 to October 2014, 38 patients with KS at the First Affiliated Hospital of Xinjiang Medical University and Urumqi Infectious Disease Hospital were enrolled, among whom 28 were male and 10 were female, and all of them were uygur.Immunohistochemical and Western blot methods were used to detect the expressions of caspase-3 and E-cad proteins in 22 cases of AIDS-KS patients and 16 cases of classic KS.The quantitative data of normal distribution were analyzed by t test, while count data were compared with χ2 test with R × C table.Results KS lesions in patients with classic KS were confined to the skin, without mucosal, lymph node or visceral involvement.Lesions in AIDS-KS patients were not only confined to the skin and superficial lymph nodes, but also oral mucosa involved in 12 cases and internal organs involved in 7 cases.Liver and lung involvement was more common.The CD4+T lymphocyte count in patients with AIDS-KS was (200.8±166)/μL.All 15 AIDS cases with CD4+ T cell count less than 200/μL developed opportunistic infections.CD4+ T lymphocyte count of patients with classic KS was (562.52±222.66)/μL and the 16 patients with CD4+T lymphocyte count greater than 350/μL had no opportunistic infections.The results of immunohistochemistry showed that the positive expression rate of caspase-3 protein in KS tissues in patients with AIDS-KS was 68.2%, in patients with classic KS was 100.0%, with significant difference between two groups (χ2=7.37, P=0.01).The positive expression rate of E-cad protein in KS tissues in patients with AIDS-KS was 72.7%, in patients with classic KS was 100.0%, with significant difference between two groups (χ2=5.18, P=0.03).Western blotting showed that the gray value of caspase-3 in the KS tissue of patients with AIDS-KS was 0.55±0.36, and that in patients with classic KS was 0.86±0.56, with significant difference between two groups (t=-2.070, P<0.05).The gray value of E-cad in the KS tissue of patients with AIDS-KS was 0.54±0.41, and that in patients with classic KS was 0.85±0.45, with significant difference between two groups (t=-2.060,P<0.05).Conclusions There are differences in the protein expressions of caspase-3 and E-cad in tumor tissues of patients with AIDS-KS and classical KS in Xinjiang Uygur patients with Kaposi's sarcoma, which may correlate with a faster progression and a higher mortality rate for AIDS-KS.

Objective To compare the traditional electrophysiological testing with modified methods for differential diagnosis of Radial Tunnel Syndrome (RTS).Methods A total of 87 selected patients were initially diagnosed as Lateral Epicondylitis (LE) or Tennis Elbow (TE) by doctors from the Outpatient Department of Orthopedics and Rehabilitation.Medical history was asked.Patients received physical examination and examinations for the sensory nerve action potential (SNAP) of superficial radial nerve,the compound muscle action potential (CMAP) of radial nerve and needle electromyography (EMG) to record the muscle Motor Unit Action Potentials (MUAPs).Then,the modified methods for CMAP of radial nerve were conducted on the forearm in the neutral,pronation and supination positions.Three values of CMAP latency were compared.RTS was diagnosed when the difference value ≥0.3 ms.The x 2 test was used to compare the positive detectable rates of the two methods for the RTS diagnosis.Results Thirteen out of 87 patients were diagnosed as RTS,among which three had interosseous nerve lesion and one had superficial radial nerve lesion.The traditional EMG failed to diagnose the remaining 9 RTS cases.These patients were finally diagnosed due to their latency difference of radial nerve CMAP ≥0.3ms when their forearms were examined in three positions.Conclusion The modified electrophysiology method shows a higher positive rate for the diagnosis of RTS.(P＜0.05).

Objectives To enhance the diagnosis of cervical epithelial neoplasia and cervical cancer at the early stage and to prevent cervical invasive carcinoma by analyzing the cervical/vaginal cytology or histopathologic results of Atypical squamous cells of undetermined significance (ASCUS).Methods From January 2014 to June 2015,a total of 4579 cases were selected in the census of gynecological diseases in Qiannan Hospital.TBS classification (the bethesda system) showed that 164 cases of ASCUS or above were found using cervical/vaginal cytology with live tissue under colposcopy.Results Among 164 cases,75 was cervical intraepithelial neoplasia level 3 (CINⅢ) accounting for 54.7％.Sixty-three cases of ASCUS and 16 of CINⅡ or above were found among patients at the age of 39 or younger (25.4％).A hundred and one cases of ASCUS and 59 of CINⅡ or above were found among women older than 40 (58.41％).Among patients with liquid-based cervical cytology positive,those older than 40 had higher risk.Liquid-based cervical cytology examination was divided into 5 levels.Spearman rank correlation analysis was applied with correlation coefficient (r =1) and positive correlation was shown (P =0.000,P＜0.01).The difference was statistically significant.The higher level of liquid-based cervical cytology examination level was required,the higher risk of cervical cancer was seen among women.Corclusion The use of liquid-based cervical cytology examination can help grassroots doctors improve CIN diagnosis rate and prevent cervical invasive carcinoma among women in the countryside.

Objective To summarize the key nursing points in patients with monochorionic twins undergoing selective feticide with bipolar electrocoagulation.Methods Selective feticide with bipolar electrocoagulation were performed in 68 monochorionic twins with one twin anomaly.And the perioperative care was performed on the patients.Results Operations were accomplished successfully in 68 cases.No complications,such as infections,premature rupture of membrane and so on,were found in all cases seven days after operation.Conclusions The nursing key points include preoperative psychological nursing by interpretation of the operations,monitoring fetal heart sounds during operations,and close observation of body temperature,uterine contractions and fetal heart rate in pregnant women after operations.Careful perioperative nursing is helpful for improvement of operative success and to ensure normal development of fetus.

Objective: 1.To analyze the nutritional composition and evaluation of the nutritional value of Mortierella isabelina powder. 2.To investigate the effects of Mortierella isabelina powder on prevention and regulation of hyperlipidemia in rats. Methods: 1. Mycelium powder was analyzed for proteins, lipids polysaccharides, fibre, ash, vitamin E, amino acids, fatty acids and minerals. 2. Mycelium powder was used to feed rats in different dosages together with high lipid diet for 10 days. 3. Mycelium powder was used to feed hyperlipidemic rats in different dosages for 30 days. Results: (1) There were about 20% of proteins and more than 50% of lipids in the mycelium. Further examination found that the mycelium contained rich essential amino acids and unsaturated fatty acids. The mycelium also contained vitamin E and useful minerals such as K, Ca, P, Fe, Zn and Mn. (2) 0.6 or 1.2 g/(kg?d) dosage of mycelium powder could prevent the rise of serum lipids. (3) When the mycelium powder was used to feed hyperlipidemic rats, the serum cholesterol, triglyceride, LDL C and VLDL C of the rats could be reduced, meanwhile HDL C could be increased. Conclusion: The results indicated that Mortierella isabelina powder has potential nutritional value and can be used to regulate the blood lipids in hyperlipidemic rats.

Objective To investigate the influence of application of local anesthesia with lidocaine on bronchial lavage fluid (BLF) pseudomonas aeruginosa culture and drug sensitivity in cases with lung infection .Methods Two hundred and seventy speci-men of BLF were collected from 135 patients with infection of lung .And BLF were collected directly from right-broncho in control group ,and from left-broncho in lidocaine group .The outcome of pseudomonas aeruginosa culture and drug sensitivity were com-pared in the two groups .Results Fourty-two cases were postitive in BLF pseudomonas aeruginosa culture in the control group ,and 40 cases were postitive in lidocaine group .The positive rates were 31 .11% and 29 .63% ,respectively .There were no significance between the two groups (P<0 .001) .Compared with the control group ,the sensitive strains of pseudomonas aeruginosa were obvi-ously less and the drug tolerance strains were much more in lidocaine group for Ciprofloxacin and Levofloxacin (P<0 .05) .Howev-er ,there were no influence for drugs such as Piperacillin/Tazobactam and Ceftazidime ,etc .Conclusion 2% lidocaine has no influ-ence on the outcome of BLF pseudomonas aeruginosa culture .But it may reduce the drug sensitivity of Ciprofloxacin and Levofloxa-cin in cases with infection of lung .