OBJECTIVE: To study the bacteriostatic action of polysaccharides of the root and stem of Rosa laevigata.METHODS: The bacteriostatic action of the polysaccharides of the root and stem of R.laevigata was determined by punching method in agar diffusion method.Four holes were punched in every medium and marked by No.1,2,3 and 4.A few drops of culture solution of bacteria or fungi were dripped to culture medium to be evenly distributed using smear bar.Hole No.1 was added with antibiotics,hole No.2 blank control,No.3 polysaccharides solution of the root of R.laevigata and No.4 polysaccharides solution of stem of R.laevigata at different concentrations;then the medium was put into incubator for culture of a period of time in a suitable constant temperature. Then the bacteriostatic circle diameter was observed.RESULTS: Polysaccharides of the root and stem of R.laevigata had inhibitory action on staphylococcus albus,staphylococcus citreus,staphylococcus aureus,standard klebsiella pneumoniae and dysentery bacillus in a dose-dependent manner.CONCLUSION: The polysaccharide of both the root and the stem of R.laevigata have bacteriostatic action;and the root and the stem of R.laevigata have no significant differences in bacteriostatic action.

Objective To study the quality control method of Feireqingjie Oral Liquid. Methods UV was used to determine the content of total flavonoid. Cortex Mori was identified qualitatively by TLC. Pheretima was identified qualitatively by PC. Results Total flavonoid showed a good linear relationship in the range of 0.005 26-0.052 60 mg/mL, r=0.999 8. The average recovery rate was 98.65%, RSD=0.28% (n=6). The TLC spot of Cortex Mori was coincident with the standard reference, and Pheretima showed the same color as the reference in PC, with no interference of negative control. Conclusion The method is convenient, accurate and can be used as one of the quality control methods of Feireqingjie Oral Liquid.