Objective To investigate the effect of myeloid differentiation factor88 (MyD88)on the experimental autoimmune myocarditis (EAM) in Balb/c mice.MethodsTotally 90 Balb/c mice were randomly divided into three groups:MyD88 ( n =30),myocarditis ( n =30),and control ( n =30 )groups.An injection of MyD88 high expression adenovirus was carried out through vena caudalis at day 3.The mice of myocarditis group received porcine cardiac myosin at the same time and position.The mice of control group received PBS only.Serum and myocardium samples were gained at day 21.The inflammatory infiltration and the serum levels of cTnI,IgG,and IgM were examined.Results 21 days later,the inflammatory infiltration score of the myocarditis group was higher than the control group (2.39 ± 1.23 vs 0.68 ±0.65,P ＜0.05).The MyD88 group was significantly higher than the other 2 groups ( F =94.194,P ＜0.01 ),the myocarditis group ( 3.56 ± 1.34 vs 2.39 ± 1.23,P ＜ 0.05 ),and the control group ( 3.56 ±1.34 vs 0.68 ±0.65,P ＜0.01).The serum levels of cTnI,IgG,and IgM of the MyD88 group were significantly higher than the myocarditis group and the control group( P ＜0.01,P ＜0.05).Conclusions MyD88 participates in the pathogenesis of experimental autoimmune myocarditis in mice.

Objective To compare the characteristics of pulmonary function between stable chronic obstructive pulmonary disease (COPD) and asthma-COPD overlap syndrome (ACO) in the elderly males. Methods Three hundred and eighteen elderly male patients with stable COPD were followed up between January 2014 and December 2017. A11 patients underwent pulmonary function tests and bronchial dilation test (BDT) in the stable condition. According to the occurrence of ACO, the patients were divided into ACO group (54 cases) and non-ACO group (264 cases). The indexes of pulmonary function before and after BDT (T0 and T1) in two groups were statistically compared. Results Among 318 elderly male patients with stable COPD, ACO patients accounted for 17.0％(54/318). Compared with non-ACO group, at T0 ACO group had significantly lower values in forced vital capacity (FVC) pred [(83.5 ± 15.4)％vs. (93.9 ± 5.6)％], in forced expiratory volume in 1 second (FEV1) pred [(61.1 ± 13.6)％vs. (74.5 ± 12.0)％], in forced expiratory volume in 3 seconds (FEV3) pred [(70.5 ± 13.4)％ vs. (81.8 ± 12.9)％] and in forced expiratory flow rat (FEF25％-75％) pred [(26.7 ± 8.5)％vs.(36.7 ± 10.2)％](P<0.05), but at T1 there were no significant differences in FVC pred, FEV1 pred, FEV3 pred and FEF25％-75％ pred between the two groups(all P>0.05). And ACO group also had significantly higher values in residual volume pred [(138.9 ± 25.7)％vs. (117.5 ± 26.6)％] and in residual volume/total lung capacity [(55.8 ± 9.7)％ vs. (45.1 ± 8.9)％] (P<0.05). Conclusions ACO is common in the elderly male patients with stable COPD, and ACO patients have lower time vital capacity as compared with non-ACO patients. But after bronchodilation test, the two groups have similar ventilation function, and the small airway function in ACO patients improves more significantly .

Objective:To analyze the correlation between cuproptosis genes and immune infiltration during the occurrence and development of multiple sclerosis(MS),and to predict the traditional Chinese medicine,to provide theoretical basis for the mecha-nism study of cuproptosis in MS immune infiltration and Chinese medicine to intervene in immune regulation.Methods:The gout chip of MS was downloaded from the GEO database and standardized;based on the processed data,immune cells and functions were ex-tracted and quantified,and the correlation and differences of immune cells and functions were analyzed;at the same time,cuproptosis genes related to MS were screened out,a risk model was constructed,and enrichment analysis was carried out;the prediction of cu-proptosis genes and immune-related biological processes were carried out.Results:① Among immune cells,T follicular helper cell and B cell showed the strongest positive correlation;among the immune functions,parainflammation and typeⅠIFN reponse showed the strongest positive correlation;②B cell,T helper cell and human leukocyte antigen were lowly expressed in MS patients,while major histocompatibility complex class Ⅰ,parainflammation and typeⅠIFN response were significantly expressed;③ The cupropto-sis genes associated with MS were SLC31A1,PDHA1,NLRP3,MTF1,GLS and DBT,of which DBT was the most likely risk factor for MS;④The occurrence and development of MS involves biological processes such as IL-4 production,T-helper cell differentiation,and acute inflammatory response,which were related to pathways such as arginine biosynthesis,citrate cycle,and propanoate metabo-lism;⑤Banxia,Danshen,Jianghuang and other traditional Chinese medicines may be used as potential molecular drug sources.Con-clusion:The expression of cuproptosis gene is closely related to MS-related immune cells and functions,which can provide support for the basic research of MS.