Objective To study the correlation between immune imbalance mediated by Th 17 and Treg cells and impaired lung function in patients with chronic obstructive pulmonary disease ( COPD ) . Methods Ninety-five patients with moderate or severe COPD , thirty-five smokers with normal lung function and thirty-one healthy non-smokers from Yinzhou People′s Hospital from January 2009 to December 2012 were recruited in this study .The percentages of circulating Th 17 and Treg cells in peripheral blood samples were determined by flow cytometry .The concentrations of cytokines in serum samples and supernatants of in-duced sputum samples were measured by enzyme-linked immunosorbent assay (ELISA).The expression of ROR-γt and Foxp3 at mRNA level in peripheral blood mononuclear cells ( PBMC) were determined by quan-titative real-time PCR.The potential association between ratios of Th 17/Treg cells and lung function was evaluated.Results Compared with smokers and healthy subjects , patients with moderate or severe COPD showed high levels of Th17 cells, IL-17A, IL-6 and IL-23, and an up-regulated expression of ROR-γt at transcriptional level .However, the percentage of Treg cells , IL-10 level and the expression of Foxp 3 at mRNA level were down-regulated in patients with COPD .The levels of cytokines in supernatants of induced sputum samples varied in the same way as that of Th 17/Treg in peripheral blood samples .The ratio of Th17 to Treg cells was negatively correlated with the values of forced vital capacity ( FVC) , forced expiratory vol-ume in one second (FEV1), and FEV1/FVC.Conclusion Th17/Treg cells imbalance was closely associ-ated with the deterioration of pulmonary function in patients with moderate or severe COPD .Th17/Treg cells imbalance might be involved in the progression of COPD .

Objective: To observe the therapeutic efficiency of adenovirus-mediated interleukin-12 gene(AdmIL-12) and CD40 ligand gene(AdmCD40L) intratumoral transfer in established murine melanoma in vivo. Methods: C57BL/6 mice were inoculated subcutaneously with B16 cells to establish the murine melanoma model. The tumor-bearing mice were injected intratumorally with murine IL-12 gene and CD40L gene recombinant adenovirus. Tumor growth and the survival of tumor-bearing mice were observed. The CTL activity was measured in vitro by lactate dehydrogenase(LDH) release assay. Results: Both AdmIL-12 and AdmCD40L can be efficiently expressed in vitro and in vivo. The treatment with AdmIL-12 could significantly inhibit the tumor growth and prolong the survival period of the tumor-beraing mice. Splenic CTL activity of the mice was also enhanced after IL-12 gene transfer. But the anti-tumor effects of AdmCD40L gene were not significant. In contrast, Co-delivery of IL-12 gene and CD40L gene lead to stronger antitumor effects than IL-12 gene alone. Conclusion: Adenovirus-mediated interleukin-12 gene and CD40 ligand gene transfer together intratumorally has significant therapeutic effects on mice melanoma in vivo.

Objective To investigate the relationship between serum HBV -DNA quantitative detection level and liver function markers and hepatitis B markers in patients with chronic hepatitis B virus. Methods The clinical data of 80 patients with chronic hepatitis B virus infection were retrospectively analyzed,and the HBV-DNA load,the serum hepatitis B markers and liver function indicators of the patients were detected and recorded. Another 80 cases received normal physical examination were selected as the control group. The serum markers were detected in both two groups. Results Of 80 patients with chronic hepatitis B virus infection,the positive rate of HBV-DNA was 45. 0% (36/80) and the detection rate of HBeAg was 30. 0% (24/80). The positive rate of HBV -DNA was positively correlated with HBeAg positive rate(χ2 =12. 46,P=0. 00). The levels of alanine aminotransferase (ALT),α-L-fucosidase (AFU),aspartate aminotransferase (AST) and plasma prothrombin time (PT) in HBV-DNA positive patients were significantly higher than those in HBV-DNA negative group( F=42. 693,18. 752,32. 692, 48. 225,all P=0. 000);and with the increase of the amount of virus,the level of the patients' index continued to increase. Conclusion For patients with chronic hepatitis B,the HBV-DNA level and the viral replication should be detected,and the liver function should be evaluated to observe the liver damage timely,thus to provide reference for clinical effective intervention.