AIM:To establish the normalized chromatographic fingerprints(NCFP) of Shegan Antiviral Injectionl(Rhizoma Belamcandae,Flos Lonicerae japonicae,Herba Artemisiae scopariae,Radix Bupleuri,etc.)(SGKBDI) and to evaluate them by using the 25 relatively normalized parameters.METHODS:The chromatographic fingerprints of the eight herbal drugs,two intermediates and preparation of SGI were determined by RP-HPLC.The 11 chromatographic fingerprints were evaluated by 25 relatively normalized index calculated by the software of Digitalized Evaluation System of Super-information Characteristic for Traditional Chinese Medicine Fingerprints 3.0.RESULTS:The best fingerprints were S4 Herba Artemislae Scopariae and S7 Radix Isatidis,the next were S2 Flos Lonicerae、 S5 Radix Bupleuri、 S6 Herba Taraxaci、 S8 Folium Isatidis,S10 intermediat Ⅱ and S11 Sgkbdi.CONCLUSION:The 25 characteristic parameters can be used to objectively,authentically and thoroughly reveal the separation of chromatographic fingerprints and its normalized characteristics of the NCFP of SGKBDI.

Exosomes are a subpopulation of extracellular vesicles ranging in size from 30 to 150 nm. They contain a variety of biological molecules including proteins, lipids, and genetic materials by which they can act as mediators of cell-to-cell communication. Increasing studies on exosomes have elucidate the mechanism of communication between immune cells. Mast cells are found to release exosomes in both resting and activated states, but the quantity, contents and functions of the exosomes are significantly different in different states. Mast cell-derived exosomes are involved in the maturation and antigen presentation of dendritic cells, and mediate the activation of T lymphocytes and the polarization of macrophages. These results provide new insights into the role of exosomes in mast cell function and immune regulation.

Prostate cancer (PCa) patients who progress to metastatic castration-resistant PCa (mCRPC) mostly have poor outcomes due to the lack of effective therapies. Our recent study established the orphan nuclear receptor ROR