Objective:To investigate the characteristics of fragility fractures of pelvis(FFP) in the elderly and compare the clinical efficacy between conservative treatment and minimally invasive surgery.Methods:A retrospective study was conducted in the 56 elderly FFP patients who had been treated at Department of Orthopaedics, The General Hospital of Chinese People＇s Liberation Army from January 2017 to January 2019. They were 16 males and 40 females, with an age of 73.4 years (from 65 to 93 years). By the American Society of Anesthesiologists (ASA) classification, there were 12 cases of grade Ⅰ, 16 cases of grade Ⅱ, 20 cases of grade Ⅲ, and 8 cases of grade Ⅳ; by the FFP classification, there were 6 cases of type Ⅰ, 10 cases of type Ⅱ, 36 cases of type Ⅲ, and 4 cases of type Ⅳ. The morphological characteristics and injury mechanisms of FFP were analyzed. According to the treatment methods, the patients were divided into a conservative treatment group of 32 cases and a minimally invasive surgery group of 24 cases. The 2 groups were compared in terms of complication incidence, mortality and the Koval attenuation rate of walking ability after one-year follow-up.Results:There were mostly the fractures of pubic branches on both sides of the pubic symphysis and compression fractures of the sacral wing caused by lateral crush injury. The 2 groups were comparable due to no significant differences in the preoperative general data between them other than FFP classification ( P>0.05). By one year after treatment, the conservative treatment group had a complication incidence of 34.4% (11/32), a mortality of 9.4% (3/32) and a Koval attenuation rate of walking ability of 13.8% (4/29) while the minimally invasive surgery group had a complication incidence of 20.8% (5/24), a mortality of 4.2% (1/24) and a Koval attenuation rate of walking ability of 8.7%(2/23), showing no significant difference between the 2 groups ( P>0.05). Conclusions:The injury mechanism of geriatric FFP is mostly lateral compression injury. The fracture sites are mostly located on both sides of the pubic symphysis, pubic branches and the sacral wing of anterior and posterior rings simultaneously. Although there may be no significant difference in complication incidence, mortality or Koval attenuation rate of walking ability between conservative treatment and minimally invasive surgery after one year, the minimally invasive surgery deals with more unstable fracture types.

Objective To analyze the impact of feedforward control nursing intervention on e-mergence agitation and recovery quality in general anesthesia surgery patients.Methods A total of 118 patients undergoing general anesthesia surgery were selected by convenient sampling,and ran-domly divided into control group and observation group,with 59 patients in each group.The control group received routine nursing intervention during emergence anesthesia,while the observation group received feedforward control nursing intervention.The incidence of emergence agitation,recovery quality,pain condition,and nursing quality were compared between the two groups.Results The incidence of emergence agitation during emergence in the observation group was significantly lower than that in the control group(42.37％versus 69.49％,P＜0.05).The Richmond Agitation-Seda-tion Scale(RASS)scores,duration and frequency of agitation were lower or shorter in the observation group than those in the control group(P＜0.05).The observation group exhibited shorter time for spontaneous respiration recovery,eye opening,extubation,full consciousness,and stay in the postan-esthesia care unit compared to the control group(P＜0.05).The Steward emergence score was higher in the observation group(P＜0.05).The postoperative pain score was lower,while nursing quality score was higher in the observation group than those in the control group(P＜0.05).Conclusion Feed-forward control nursing intervention can reduce the incidence of emergence agitation in general anesthesia surgery patients,improve recovery quality,alleviate postoperative pain,and enhance nursing quality.

Objective To analyze the impact of feedforward control nursing intervention on e-mergence agitation and recovery quality in general anesthesia surgery patients.Methods A total of 118 patients undergoing general anesthesia surgery were selected by convenient sampling,and ran-domly divided into control group and observation group,with 59 patients in each group.The control group received routine nursing intervention during emergence anesthesia,while the observation group received feedforward control nursing intervention.The incidence of emergence agitation,recovery quality,pain condition,and nursing quality were compared between the two groups.Results The incidence of emergence agitation during emergence in the observation group was significantly lower than that in the control group(42.37％versus 69.49％,P＜0.05).The Richmond Agitation-Seda-tion Scale(RASS)scores,duration and frequency of agitation were lower or shorter in the observation group than those in the control group(P＜0.05).The observation group exhibited shorter time for spontaneous respiration recovery,eye opening,extubation,full consciousness,and stay in the postan-esthesia care unit compared to the control group(P＜0.05).The Steward emergence score was higher in the observation group(P＜0.05).The postoperative pain score was lower,while nursing quality score was higher in the observation group than those in the control group(P＜0.05).Conclusion Feed-forward control nursing intervention can reduce the incidence of emergence agitation in general anesthesia surgery patients,improve recovery quality,alleviate postoperative pain,and enhance nursing quality.

Perilla (Perilla frutescens L.) is an important edible-medicinal oil crop, with its seed containing 46%-58% oil. Of perilla seed oil, α-linolenic acid (C18:3) accounts for more than 60%. Lysophosphatidic acid acyltransferase (LPAT) is one of the key enzymes responsible for triacylglycerol assembly in plant seeds, controlling the metabolic flow from lysophosphatidic acid to phosphatidic acid. In this study, the LPAT2 gene from the developing seeds of perilla was cloned and designated as PfLPAT2. The expression profile of PfLPAT2 gene was examined in various tissues and different seed development stages of perilla (10, 20, 30, and 40 days after flowering, DAF) by quantitative real-time PCR (qRT-PCR). In order to detect the subcellular localization of PfLPAT2 protein, a fusion expression vector containing PfLPAT2 and GFP was constructed and transformed into Nicotiana benthamiana leaves by Agrobacterium-mediated infiltration. In order to explore the enzymatic activity and biological function of PfLPAT2 protein, an E. coli expression vector, a yeast expression vector and a constitutive plant overexpression vector were constructed and transformed into an E. coli mutant SM2-1, a wild-type Saccharomyces cerevisiae strain INVSc1, and a common tobacco (Nicotiana tabacum, variety: Sumsun NN, SNN), respectively. The results showed that the PfLPAT2 open reading frame (ORF) sequence was 1 155 bp in length, encoding 384 amino acid residues. Functional structure domain prediction showed that PfLPAT2 protein has a typical conserved domain of lysophosphatidic acid acyltransferase. qRT-PCR analysis indicated that PfLPAT2 gene was expressed in all tissues tested, with the peak level in seed of 20 DAF of perilla. Subcellular localization prediction showed that PfLPAT2 protein is localized in cytoplasm. Functional complementation assay of PfLPAT2 in E. coli LPAAT mutant (SM2-1) showed that PfLPAT2 could restore the lipid biosynthesis of SM2-1 cell membrane and possess LPAT enzyme activity. The total oil content in the PfLPAT2 transgenic yeast was significantly increased, and the content of each fatty acid component changed compared with that of the non-transgenic control strain. Particularly, oleic acid (C18:1) in the transgenic yeast significantly increased, indicating that PfLPAT2 has a higher substrate preference for C18:1. Importantly, total fatty acid content in the transgenic tobacco leaves increased by about 0.42 times compared to that of the controls, with the C18:1 content doubled. The increased total oil content and the altered fatty acid composition in transgenic tobacco lines demonstrated that the heterologous expression of PfLPAT2 could promote host oil biosynthesis and the accumulation of health-promoting fatty acids (C18:1 and C18:3). This study will provide a theoretical basis and genetic elements for in-depth analysis of the molecular regulation mechanism of perilla oil, especially the synthesis of unsaturated fatty acids, which is beneficial to the genetic improvement of oil quality of oil crops.
Acyltransferases ; Cloning, Molecular ; Escherichia coli/metabolism* ; Fatty Acids ; Perilla frutescens/metabolism* ; Plant Oils ; Plant Proteins/metabolism* ; Saccharomyces cerevisiae/metabolism* ; Seeds/chemistry* ; Tobacco/genetics*