Objective To explore the changes in serum concentrations of angiogenic factors in patients with unstable angina pectoris.Methods A total of 60 patients with unstable angina pectoris was eligible for study and divided into diabetes group ( A group, n =20) and non-diabetes group (B group, n =40).Another 30 general-matched healthy subjects from medical examination center were enrolled as control group .Serum samples were collected , and serum concentrations of vascular endothelial growth factor (VEGF), angiopoietin-1, angiopoietin-2, angiogenin, angiostatin, basic fibroblast growth factor (bFGF), and platelet-derived growth factor-BB ( PDGF-BB) were measured by cytokine array technology and compared between the groups .Results Compared with the control group, the serum concentrations of VEGF [(325.2 ±210.1)pg/ml] and angiopoietin-2 [(3031.3 ±1865.5)pg/ml] were sig-nificantly increased in patients with unstable angina pectoris (both P <0.05).Whereas,no significant differences in serum concentra-tions of angiogenin,angiopoietin-1,angiostatin,bFGF,and PDGF-BB were detected between control group and patient groups .There were no significant differences in serum concentrations of above all 7 biomarkers between diabetes group and non-diabetes group .Con-clusions Serum concentrations of VEGF and angiopoietin-2 were increased in patients with unstable angina pectoris ,and diabetes didn't affect the increases in serum concentrations of VEGF and angiopoietin-2 caused by unstable angina pectoris .

Objective:To find out the characteristic topog ra phic distribution of the muscle spindles in human masseter.Methods: The muscle spindles of masseter on both sides of 5 human corpses fixed in for malin for more than 1 year were observed by histological technique and quantitat ively analyzed by picture analysis equipment.Results: The muscle spindles were maldistributed and the spindles were commonly clustered in some i ndividual portions,and the muscle spindles figures of the masseter were diversif orm,which were predominantly composed of the spindle simple and spindle complexe s. The spindle complexes in the deep portion was obviously more than that in the superficial. The muscle spindles in the muscle close to the temporomandibular j oint and muscle tendon in the deep portion were more than that in the other port ions.Conclusion:The distribution of muscle spindles in the masse ter is heterogeneous,muscle spindle density in the deep portion is greater than that in the superficial.

BACKGROUND:How to effectively improve the airway management and improve oxygen supply at the same time through a simple and effective method becomes an operation key for physicians in fiberoptic bronchoscopy. OBJECTIVE:To observe the clinical effect of self-made simple masks in painless fiberoptic bronchoscopy. METHODS:A total of 60 patients receiving painless fiberoptic bronchoscopy, 32 males and 28 females, with a mean age of (57.8±10.9) years were randomized into two groups: test group with self-made simple mask during painless fiberoptic bronchoscopy and control group with laryngeal mask. In the course of examination, the changes of oxygen saturation, mean arterial pressure and heart rate were observed at different time points. And adverse events of both groups were also recorded. RESULTS AND CONCLUSION:Patients from both groups successfuly completed fibre bronchoscopy. The oxygen saturation, mean arterial pressure and heart rate in the two groups were not significantly different (P > 0.05). However, the test group (one case of bucking) was superior to the control group (seven cases of sore throat) in terms of adverse events (P < 0.05). The simple mask can improve the oxygen supply and reduce the adverse events.

Objective: To study the architectural features and the biomechanical properties of human masseter and temporolis muscles. Methods: The architecture parameters of masseter and temporolis muscles on both sides of 5 human corpes fixed in formalin for more than 1 year were measured and quantitatively analyzed. Results: Human masseter and temporolis muscles were multipennate, there existed many layers of aponeurosis to which muscle fibers attached especially within the superficial portion of the masseter. The parameters of superficial portion of the masseter were statistically different from those of the deep portion, so were those of anterior portion of temporolis from those of posterior portion; the posterior part of the temporolis was the heaviest, the deep of the masseter the lightest. The physiological cross-sectional area of the superficial part of masseter was the largest, the posterior of temporolis the second, the anterior of temporolis the third, and the deep of the masseter the smallest. Among the ratios of muscle weight vs physiological cross-sectional area in each portion of the masseter and temporolis, the ratio in the deep part of masseter was the largest, that in the posterior of temporolis the smallest, that in the superficial of the masseter was similar to that in the anterior of the temporolis. In addition, the fiber in the posterior of the temporolis was the longest and that in the deep of the masseter the shortest. Conclusion: The deep portion of masseter is designed for force production, the posterior of the temporolis for velocity production, and the superficial of the masseter and the anterior of the temporolis for both force and velocity production. The deep part of masseter and the anterior of temporolis play an important part in maintaining mandibular position and balence, and the superficial of masseter and the posterior of temporolis play a major role in producing occlusive force and mandibular fast retrusion.

Objective To study the immune response gene and the mechanism of membranous glomerulonephritis in the mouse. Methods Reproduced and identitied the animal model of MGN in mice ,extracted the total RNA of pathology group and the control group,amplified and verified I-Aβ1 gene through RT-PCR. Then sequenced and analyzed the I-AβI gene from the PCR production. Results The mutation rate of the I-Aβ1 gene was 2. 578‰ in the pathology group, and 0. 286‰ in the control group. It was obviously higher in the pathology group than in the control group(P ＜0.01). Conclusion I-Aβ1 gene rose in mice,may be related to membranous glomerulonephritis.

Objective To observe the dynamic histological changes of condylar cartilage ,and detect expression of estrogen re‐ceptors and collagen typeⅡin the ovariectomized rat ,exploring the relationship between OA and abnormity of shorting estrogen . Methods Histological changes of condylar cartilage of the ovariectomized rat at different ages were observed by HE staining meth‐od .The expression of estrogen receptors and collagen typeⅡwere determined by immunohistochemical method ,and the average per‐centage of positive staining area was measured by image analyzer .Results The lack of estrogen led to the degeneration of condylar cartilage .The expression of ER and ColⅡ were suppressed at low concentration of estrogen ,and these effects were enhanced when the treating time was prolonged .Conclusion The lack of estrogen might lead to the rat′s condylar cartilage lesion ,and the low con‐centration of estrogen could suppress the expression of ER .These results highlight the results that expression of condylar cartilage collagen is associated with estrogen .

lsartan could reduce the release of p-selectin.Conclusions Valsartan could relieve myocardial ischemia reperfusion injury of rat, which may be through reducing p-seleetin of plasma.

Objective To observe the effects of Gross Saponins of Tribulus terrestris(GSTT)on energy metabolism and lactic acid metabolism in hyperlipemic rats with acute myocardial infarction(AMI).Methods Rats were fed with high fat diet for 15 days to induce hyperlipidemia,then left anterior descending branch of coronary artery of rats were ligated.Rats showed ST segment elevation in lead Ⅱof electrocardiogram were proved to be AMI rats.AMI rats with hyperlipidemia were divided randomly into five groups(normal group,model group,24.3 mg?kg-1 GSTT group,12.15 mg?kg-1 GSTT group,Simvastatin group)and test drugs were given in succession for 28 days.Left ventricular ischemic myocardium were harvested to prepare myocardial mitochondria.Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity and lactic acid content were measured.Results Compared with the normal group,Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity significantly lowered in model group(P

Objective To understand the status and influencing factors of benefit finding in patients with cancer.Methods 350 patients with cancer were collected with convenience sampling method from two Jiangsu hospitals.All patients received a questionnaire survey.Multiple linear regression analysis was used to analyze the influencing factors of benefit finding in patients with cancer.Results (1) The score of benefit finding in patients with cancer was statistically significant in different frequency of physical exercise (F=8.37,P=0.00),different character (F=3.04,P=0.04) and different educational level (F=2.91,P=0.03).(2) There was a positive correlation between benefit finding(78.53± 10.50) and communication with physicians(7.27±2.19),medical coping(43.48±4.50),optimism(17.33±2.24) and social support(44.81±3.91)(r=0.31,0.35,0.29,0.23,all P＜0.05) in patients with cancer.The benefit finding in patients with cancer was negatively correlated with depression(8.59± 1.83) (r=-0.37,P＜0.05).(3) Multiple linear regression analysis results showed that medical coping modes-confrontation,objectively social support and utilization,physical exercise,depression,communication with physicians and character were the factors influencing benefit finding in patients with cancer(△R2 =28％,P＜ 0.05;△R2 =5.5％,P＜ 0.05;△R2 =3.1％,P＜ 0.05;△R2 =1.9％,P＜0.05;△R2 =1.7％,P＜0.05;△R2 =1.0％,P＜0.05;△R2 =1.1％ P＜0.05),which explained 42.0％ of the total variance.Conclusion Medical coping modes-confrontation,objectively social support and utilization,physical exercise,depression,communication with physicians and character are the factors influencing benefit finding in patients with cancer.

A new method using on-line cleanup technology combined with liquid chromatography tandem mass spectrometry ( UHPLC-MS/MS) was developed for the determination of seven kinds of β2-agonists residues, Formoterol, Salmeterol, Carbuterol, Clenisopenterol, Clenpenterol, Clencyclohexerol and Clenbuterol-hydroxymethyl in livestock manure. The sample was sufficiently extracted by acidic acetonitrile and diluted by 0. 2% formic acid. The extract was online purified on HyperSep Retain CX column where the sample matrix was washed away and the analytes were retained. The analytes were eluted into Hypersil Gold C18 column by 2% Ammonia-methanol solution. The seven β2-agonists were detected in selected reaction monitoring ( SRM) mode via positive electrospray ionization ( ESI+) by liquid chromatography-tandem mass spectrometry. The results showed good linearity correlation coefficients over 0 . 9928 for the seven kinds of β2-agonists in 0 . 5-100 μg/L concentration range. The LOD of seven kinds of β2-agonists in livestock mature is 1 μg/kg, while the LOQ is 5 μg/kg. When 5-50 μg/kg of the seven kinds ofβ2-agonists were added into the blank livestock manure, an average recovery of 67% -112% was obtained with the relative standard deviations of 2. 9%-10. 2%. The method is simple, rapid and has good reproducibility for quantitative and confirmatory analysis ofβ2-agonist residues.