Objective: To establish quality standard on Qinggan Granules (Radix Actinidiae valvatas, Radix Scutellariae, Rhizoma Atractylodis Macrocephalae, Badix Bupleuri, etc.). Methods: Radix Actinidiae valvatae, Radix Scutellariae, Rhizoma Atractylodis Macrocephalae and Radix Bupleuri were identified by TLC, and the content of Baicalin was determined by dual wavelength TLC scanning. Results: Baicalin showed a linear relationship at the range of 0.55～4.4?g, the average recovery was 102.9%. Conclusion: Method is simple, quick and good reproducible.

Objective:To study the epidemiological features of internet addiction disorder(IAD)in high school students in Beijing and explore the related factors.Methods:A stratified cluster sampling method was used.Totally 5100 high schools students were measured with the Internet Addiction Scale,Personality Diagnostic Questionnaire and Egna Minnen av Bardndoms Uppfostran(EMBU).Results:(1)The prevalence of IAD was 8.8% in high school students in Beijing.(2)Logistic regression analysis indicated that the risk factors of IAD were male,school area in urban area,parents' divorce,parents' refusal,parents' over-protection,paranoid personality disorder,avoidant personality disorder,antisocial personality disorder and histrionic personality disorder.The protective factor was parents' emotional warmth.Conclusion:It should be paid more attention to internet addiction disorder IAD in high school students in Beijing.Prevention and intervention should be conducted to the risk factors of internet addiction disorder IAD.

Objective:To observe the clinical efficacy of tuina plus ultrasonic therapy in treating infantile muscular torticollis. ＜br> Methods:Seventy kids with muscular torticollis were intervened by tuina plus ultrasonic therapy, and the efficacy was evaluated after 8-month treatment. ＜br> Results: After 8-month treatment, 41 subjects were cured, accounting for 58.6%, 27 were improved, occupying 38.6%, 2 failed, occupying 2.8%, and the total effective rate was 97.2%. ＜br> Conclusion: Tuina plus ultrasonic therapy can produce a significant efficacy in treating infantile muscular torticollis, without adverse effects.

Background:High-fat diet leads to intestinal mucosa barrier dysfunction,but the mechanism is not clear. High-fat diet can induce increase of bile acid. Aims:To investigate whether the high bile acid induced by high-fat diet could act on intestinal stem cell to disrupt stem cell differentiation and imparing the intestinal mucosa. Methods:Twenty-four rats were divided into 3 groups:fed with regular diet,high-fat diet and high-fat diet + cholestyramine,respectively,for 2 weeks. Serum bile acid was detected by ELISA. Ileal diameter was measured and HE staining was performed to observe histology of intestinal mucosa. Expression of Lgr5 gene was determined by RT-qPCR. Ileal tissue fed with regular diet was cultured with deoxycholic acid(DCA)or DCA + cholestyramine for 24 hours in vitro,expression of Lgr5 gene was determined by RT-qPCR. Results:Compared with control group,serum bile acid was significantly increased(P < 0. 05),ileal diameter was significantly decreased,height of intestinal crypts and villus was significantly decreased(P < 0. 05),and expression of Lgr5 gene was significantly decreased in high-fat diet group(P < 0. 01). All the above-mentioned indices were significantly ameliorated in high-fat diet + cholestyramine group(P < 0. 05). In vitro study showed that expression of Lgr5 gene was significantly decreased in DCA group than in control group(P < 0. 01),and cholestyramine could significantly increase expression of Lgr5 gene(P < 0. 05). Conclusions:High-fat diet induced increasing of circulatory bile acid can cause injury of intestinal mucosa by inhibiting stem cell function,which can be ameliorated by cholestyramine.

Objective:To observe the clinical efficacy of warm needling plus acupoint sticking therapy for primary dysmenorrhea.
Methods:A total of 60 eligible cases were randomly allocated into a treatment group (n=30) and a control group (n=30) according to their sequence of consultation. Patients in the treatment group were treated with warm needling combined with acupoint sticking therapy, whereas patients in the control group were treated with oral Ibuprofen (Fenbid) capsules. After two menstrual cycles, the visual analogue scale (VAS) score, COX menstrual symptom scale (CMSS) and clinical efficacy were compared between the two groups.
Results:After treatment, the VAS and CMSS scores were significantly reduced in both groups, indicating that both treatment protocols can remarkably alleviate pain. There was a between-group statistical difference in comparing the CMSS score (P<0.05), but no between-group statistical difference in the VAS score (P>0.05). However, the follow-up visit showed between-group statistical differences in comparing VOA and CMSS scores (bothP<0.01). The clinical effect, relapse rate and long-term efficacy in the treatment group were better than those in the control group.
Conclusion:Warm needling plus acupoint sticking therapy is a simple but effective therapy for primary dysmenorrhea. In addition, it causes fewer cases with relapse.

Objective:To evaluate the safety of the combination of cefotiam sodium and furosemide and to provide the reference for clinical medication. Methods:Three groups were established, including the blank control group, cefotiam sodium group at the dosage of 500 mg·kg-1 ·d-1 , cefotiam sodium combined with furosemide group at the respective dosage of 500 mg·kg-1 ·d-1 and 15 mg ·kg-1 ·d-1 . After the continuous administration for 12 days, the renal structure, serum uric acid, creatinine and urea nitrogen, u-rine of α1 microglobulin and β2 microglobulin in the rats were detected. Results:Cefotiam sodium at the dosage of 500 mg·kg-1 · d-1 showed no significant effects on the renal structure, serum uric acid, creatinine and urea nitrogen,urineα1 microglobulin andβ2 microglobulin in the rats. The combination group showed significantly increased urine β2 microglobulin (P<0. 05) and significantly decreased serum uric acid (P<0. 05). Conclusion:Short time use of cefotiam sodium exhibits no significant effect on the renal struc-ture and function in rats, while the combination of cefotiam sodium and furosemide has significant effects on urineβ2 microglobulin and serum uric acid in rats.

Objective To evaluate the clinical efficacy of acupuncture plus acupoint application in treating irritable bowel syndrome with diarrhea (IBS-D).Method Sixty patients were randomized into two groups, 30 cases in each group. The acupuncture group was intervened by acupuncture at Neiguan (PC6), Zhongwan (CV12), and Zusanli (ST36), etc, plus application withBai Jie Zi(Semen Brassicae) powder at Qihai (CV6), Guanyuan (CV4), and Tianshu (ST25), etc; the medication group was prescribed with oral administration of Trimebutine maleate capsules (0.2 g each dose, 3 times a day). Four-week treatment was taken as a course. The symptoms score and quality of life (QOL) scale were evaluated prior to and after the treatment.Result The clinical symptoms were significantly improved in the acupuncture group, and the total effective rate was89.3%, significantly higher than 63.0% in the medication group (P<0.05). After 4-week treatment, the clinical symptoms scores of the two groups were analyzed by rank-sum test (Z=-19.627,P=0.000<0.05), revealing a significant difference between the two groups and indicating that the improvements of clinical symptoms in the acupuncture group were more significant than those in the medication group. After 4-week treatment, the general QOL scores of the two groups were examined by rank-sum test (Z=-10.039, P=0.000<0.05), revealing a significant difference between the two groups and indicating that the improvement of QOL in the acupuncture group was superior to that in the medication group.Conclusion Acupuncture plus acupoint application can obviously mitigateabdominal pain and discomfort.

Objective: To investigate the prevalence of affective disorder in Kunming City. Methods: A rep-resentative sample of the Kunming general population composed of 5033 individuals aged 15 years and older was se-lecteed randomly by the method of probability proportional to size (PPS), and then was interviewed with the World Health Organization Composite International Diagnostic Interview Version 2.1 (CIDI, version 2.1).The preva-lence of affective disorder was investigated. And the relative factors, onset age and comorbid were also analyzed. Results: The 30-duy prevalence of affective disorder was 0.98%, the 12-month prevalence was 1.22%,and the lifetime prevalence was 1.89%. The results of the single factor analysis showed that the urban residents, the persons who divorced, limited divorced or lost one's spouse, and the unemployed or the higher educated were more vulnerable to depression. The results of the multifactor analysis showed that the rural residents and the males were protective factors to affective disorder. Different subunits of affective disorder had different onset ages. The onset age of 90% bipolar disorder patients was before 25 years old. The patients before 50 years old was risky to dysthy-mia. The patients with major depression had a lifetime risk of onset. Anxiety disorder and pain disorder would in-crease the risk of affective disorder. Conclusion: The findings show that the prevalence of the affective disorder is lower than the same kind survey abroad while it is close to the domestic's. More attention and intervention should been given to the urban population, the females, the singles, the unemployed, and the higher educationed.

Objective To investigate the relationship between metabolic syndrome (MS) and education level in Su-Xi-Chang area to provide evidence for healthcare policy making. Methods A clustered sampling of 6798 subjects from Su-Xi-Chang areas of Jiangsu Province was included. Questionnaires and physical examinations were completed to collect clinical data. Logistic regression was used to analyze weather education level could be an independent risk factor of MS. Results Significant differences were found in waist circumstance (WC) ,systolic blood pressure (SBP) and fasting blood glucose (FBG) among participants with different education level. Significant differences were also existed in triglyceride and diastolic blood pressure (DBP) between subjects with higher and lower education level ( P＜0. 05 ). In single-factor or multivariate analysis, education level was negatively correlated with the prevalence of MS (P＜0. 05 ). Conclusions Education level may be an independent risk factor of MS. People with low and moderate education level have higher risk of MS. Therefore,it is recommended that health-related information should become an integral part of the compulsory education system.

Objective: To screen out suspected risk factors associated with neurosis, such as personality dis-order, life events, parental rearing behavior, and to explore degree of their associations. Methods: A case-control study was carried out among 100 cases with neurosis and 200 controls without mental disorder, matched by gender and age (+3 years) . The Life Events Scale (LES), Parental Rearing Style Questionnaire (EMBU), Personali-ty Diagnostic Questionnaire (PDQ-4) were self-reported by the subjects. Results: Single factor analysis showed that there were statistical differences (P <0.05) between case and control groups in negative life event (22.5 vs. 2.5), paternal rejection [(40.4±14.1) vs.(35.4±7.9)], paternal overprotection [(29.1±7.5)vs.(28.2±5.6)], maternal rejection rearing behavior [(40.4±13.7)vs.(36.8±8.5)], and overall personality disorder [(29.8±14.1)vs.(17.1±13.0)] . Using non-conditional logistic regression analysis, the potential protective factor was positive events (OR=0.92, 95% CI:0.87-0.98), and the potential risk factors included negative live events (OR=1.06, 95% CI:1.04～1.08) and overall personality disorder (OR=4.84, 95% CI: 2.24～10.49) . Conclusion: Positive life event may be a protective factor for neurosis, while negative life e-vent and personality disorder may be risk factors.