AIM To study the calcium antagonistic effects of Magnesium salicylate in central nervous system. MTEHODS Using calcium sensitive fluorescence indicator Fura-2 technique. RESULTS Resting [Ca 2+ ] i in brain cells under different extracellular calcium concentration(0,0 01,0 1,1 0,1 3 mmol?L -1 )were 41?5,59?6,84 7?2 5,104?7,(126?5)nmol?L -1 respectively( n =8～47).MgS 0 2 mmol?L -1 had no significant effects on resting [Ca 2+ ] i.At the same time, MgS (0 05～0 4 mmol?L -1 ) concentration dependently inhibited the [Ca 2+ ] i elevation induced by high extracellular potassium or L -glutamate in the presence of extracellular [Ca 2+ ] i 1 3 mmol?L -1 . The IC 50 values were 0 323 mmol?L -1 (95% CI 0 122～0 854 mmol?L -1 ) and 0 124 mmol?L -1 (95% CI 0 073～0 210 mmol?L -1 ) separately. CONCLUSION MgS possesses inhibitory effects on voltage-activated calcium channel and receptor-operated calcium chanel in CNS.

AIM To investigate the effects of taurine(TAU) and propafenone(PRO) combination on experimental arrhythmias and its influence on haemodynamics of anesthesized rats' heart. METHODS Rats were pretreated respectively with intravenous administration of saline, TAU, PRO, or TAU and PRO combination. Afterwards, the rats were intravenously administrated with BaCl 2 or aconitine to induce arrhythmia. The arrhythmia lasting time and incidence were observed and recorded on the lead Ⅱ ECG. The cumulative aconitine dosage to induce premature ventricular contraction(PVC), ventricular tachycardia(VT) and ventricular fibrillation(VF) were calculated respectively. The haemodynamic parameters such as LVSP, HR, +d p /d t max were recorded continuously in anesthetized rats pretreated with iv TAU, PRO or TAU and PRO combination. RESULT In BaCl 2 arrhythmia, the arrhythmia duration was shortened significantly; and the arrhythmia incidence was decreased in the combination group, as compared with the single same dosage drug groups. In aconitine arrhythmia, the cumulative aconitine dosages to induce PVC, VT and VF were increased significantly in the combination group. Haemodynamics parameters showed that PRO(2mg?kg -1 iv) significantly decreased LVSP and +LVd p /d t max , while these parameters were not significantly changed in the combination group with same dose of PRO. CONCLUSION TAU and PRO have synergism in antiarrhythmia in both BaCl 2 and aconitine induced arrhythmia; TAU may alleviate cardiac depression exerted by PRO.

OBJECTIVE:To provide reference for rational drug use in pediatric department. METHODS:The utilization of pe-diatric prescription drugs in inpatient department of our hospital during 2008 to 2014 was analyzed in respects of number,consump-tion sum,DDDs,etc. RESULTS:The consumption sum of antimicrobial drugs increased from 657 000 yuan in 2008 to 1 453 000 yuan in 2014. The consumption sum of creatine phosphate sodium increased from 384 000 yuan in 2012 to 889 000 yuan in 2014. The consumption sum of Lysine hydrochloride and sodium chloride injection entered the top 5 in 2014,reaching 205 000 yuan. The consumption sum of essential medicines changed greatly due to varieties. Top 5 antimicrobial drugs in the list of DDDs mainly wereβ-lactam,showing a descreasing trend on the whole. CONCLUSIONS:There are some problems in use of part drugs. It is need to strengthen propaganda and intervention of rational drug use.

Objective:To observe the clinical efficacy of warm needling plus acupoint sticking therapy for primary dysmenorrhea.
Methods:A total of 60 eligible cases were randomly allocated into a treatment group (n=30) and a control group (n=30) according to their sequence of consultation. Patients in the treatment group were treated with warm needling combined with acupoint sticking therapy, whereas patients in the control group were treated with oral Ibuprofen (Fenbid) capsules. After two menstrual cycles, the visual analogue scale (VAS) score, COX menstrual symptom scale (CMSS) and clinical efficacy were compared between the two groups.
Results:After treatment, the VAS and CMSS scores were significantly reduced in both groups, indicating that both treatment protocols can remarkably alleviate pain. There was a between-group statistical difference in comparing the CMSS score (P<0.05), but no between-group statistical difference in the VAS score (P>0.05). However, the follow-up visit showed between-group statistical differences in comparing VOA and CMSS scores (bothP<0.01). The clinical effect, relapse rate and long-term efficacy in the treatment group were better than those in the control group.
Conclusion:Warm needling plus acupoint sticking therapy is a simple but effective therapy for primary dysmenorrhea. In addition, it causes fewer cases with relapse.

Objective To analyse the clinicopathological characteristics of synovial sarcoma (SS) from histomorphology,immunohistochemical (IHC) and fluorescence in situ hybridization (FISH),and to investigate the related factors influencing the prognosis of SS patients.Methods 94 cases were collected,including 60 SS cases and 34 in dined to SS cases.The expressions of common antibodies related to the diagnosis of SS Vimentin,CD99,AE1/AE3,EMA,CD34,Calponin,Ki-67 were detected by IHC,and the SYT-SSX fusion gene was detected by FISH.The clinical pathologic factors that affecting the prognosis of patients were analyzed through the statistical method.Results The positive rates of Vimentin,CD99,AE1/AE3,EMA,CD34,Calponin,Ki-67 were 100.00 ％,74.47 ％,36.17 ％,28.72 ％,17.02 ％,90.43 ％ and 60.64 ％ respectively.FISH results showed that 81 patients were tested with SYT gene translocation,including 54 cases who were considered as SS,and 27 cases who were suspected/inclined to SS,and the number of positive cells surpassed 80 ％.The gene translocation was not related with the patient' s age,sex,tumor position,histological type,differentiation of the cancer (all P ＞ 0.05).Single factor survival analysis showed that the degree of differentiation,tumor diameter,the recurrence and metastasis had certain influences on patients' survival time (all P ＜ 0.05).Multiple factors regression analysis showed that the degree of differentiation and tumor diameter were the risk factors influencing the prognosis of SS (risk coefficients ＞ 1,P ＜ 0.05).Conclusions IHC combined with FISH SYT-SSX fusion gene detection have the vital significance in the diagnosis of SS.The degree of differentiation and tumor size are the risk factors influencing the prognosis of SS.

AIM: To investigate the effects of the sera from the rats after limb ischemic preconditioning (LIPC) on human umbilical vein endothelial cells (HUVECs) injured by hydrogen peroxide (H2O2).METHODS:The HUVECs were divided into 5 groups:the cells in control group were cultured without any intervention;the cells in model group (M) were damaged by 1 mmol/L H2O2 for 2 h;the cells in early preconditioning serum (EPS) group, delayed pre-conditioning serum (DPS) group or sham limb ischemic preconditioning serum (SPS) group were treated with the corre-sponding serum at 5%for 12 h, respectively , and then treaed with H 2 O2 for 2 h.The viability of the HUVECs was ana-lyzed by flow cytometry .The lactate dehydrogenase ( LDH) in the culture media was detected .The cell adhesion molecules in the HUVECs were detected by real-time PCR.The mRNA and protein expression of heme oxygenase-1 (HO-1) was also determined .RESULTS:The viability of HUVECs incubated with 1 mmol/L H2 O2 for 2 h significantly decreased compared with the control cells , which was accompanied with the augmentations of LDH in the medium and the cell adhesion mole -cules in cells , such as vascular cell adhesion molecule-1 ( VCAM-1 ) and intercellular adhesion molecule-1 ( ICAM-1 ) . Preincubation with EPS and DPS derived from the rats subjected LIPC attenuated these injuries .Furthermore, pretreatment with EPS and DPS increased the expression of HO-1 at mRNA and protein levels .CONCLUSION:LIPC protects the HU-VECs from H2O2-induced injury.

Aim To observe the effects of pituitrin ad- ministrated intravenously on electrocardiogram (ECG), and the effects of taurine on the ECG changes induced by pituitrin in conscious unrestrained rats. Methods ECG(leadⅡ)was recorded telemetrically and analyzed with Data Science International (DSI, USA); the changes of the ECG T wave and the heart rate at different times after the intravenous(iv) injection of pituitrin with or without intraperitoneal (ip) pre-administration of taurine were observed and calculated. Results ① Pituitrin 0.25～4.0 U?kg -1 dose-dependently elevated the ECG T wave; the T wave values were increased 0.75～3.42 times over the values before pituitrin, and the maximal effect appeared at 15 s after the injection. ② TAU 100, 200 or 400 mg?kg -1,injected intraperitoneally 30 min before the pituitrin, inhibited the T wave elevation and heart rate decrease induced by intravenous injection of pituitrin; compared with NS+pituitrin group, T wave elevation(mV) was decreased from 1.77?0.165 to 1.60?0.13(P

Aim To investigate the effects of topiramate (TPM) o n the model of seizure rats induced by penicillin and explore its mechanism of ant iconvulsant action.Methods Using the model of seizure rats indu ced by penicillin localized injected in cortex, we investigated the effect of TP M on the changes of seizure extent and recorded the latency of epileptiform disc harge, frequency of epileptiform wave, highest wave of hippocampus EEG. The leve ls of Glu, Asp, Gly and GABA in hippocampus were determined by high performance liquid chromatography (HPLC). Results Compared with the model g roup, TPM (110 mg?kg -1, 440 mg?kg -1, ig) could significantly light ened the extent of seizure, prolonged the latency of epileptiform discharge, red uced the frequency of epileptiform wave and minished the highest wave of hippoca mpus EEG (P

Objective To study the effect of humic acid (HA) and soil on dissolution performance of depleted uranium (DU).Methods Using the static dissolve-adsorption experiment and inductively coupled plasma-mass spectrometry(ICP-MS) to determine uranium concentration and 235U/238U isotope ratios of the samples at different time and study the dissolution of DU in water.Results The solubility of DU in water was reduced by 90％ by adding HA.Soil could increase the solubility of DU in water by nearly 25％,adding an appropriate amount of HA could play a supporting role on the dissolution of DU,in this experiment adding 5％ of HA was best.Conclusions Soil and HA could produce positive and negative impact on the solubility of DU in water,and the combined effect of the two relied on the complex absorption and complexation reactions of soil,HA and dissolved uranium ions.

Objective:To evaluate the safety of the combination of cefotiam sodium and furosemide and to provide the reference for clinical medication. Methods:Three groups were established, including the blank control group, cefotiam sodium group at the dosage of 500 mg·kg-1 ·d-1 , cefotiam sodium combined with furosemide group at the respective dosage of 500 mg·kg-1 ·d-1 and 15 mg ·kg-1 ·d-1 . After the continuous administration for 12 days, the renal structure, serum uric acid, creatinine and urea nitrogen, u-rine of α1 microglobulin and β2 microglobulin in the rats were detected. Results:Cefotiam sodium at the dosage of 500 mg·kg-1 · d-1 showed no significant effects on the renal structure, serum uric acid, creatinine and urea nitrogen,urineα1 microglobulin andβ2 microglobulin in the rats. The combination group showed significantly increased urine β2 microglobulin (P<0. 05) and significantly decreased serum uric acid (P<0. 05). Conclusion:Short time use of cefotiam sodium exhibits no significant effect on the renal struc-ture and function in rats, while the combination of cefotiam sodium and furosemide has significant effects on urineβ2 microglobulin and serum uric acid in rats.