Objective: To establish quality standard on Qinggan Granules (Radix Actinidiae valvatas, Radix Scutellariae, Rhizoma Atractylodis Macrocephalae, Badix Bupleuri, etc.). Methods: Radix Actinidiae valvatae, Radix Scutellariae, Rhizoma Atractylodis Macrocephalae and Radix Bupleuri were identified by TLC, and the content of Baicalin was determined by dual wavelength TLC scanning. Results: Baicalin showed a linear relationship at the range of 0.55～4.4?g, the average recovery was 102.9%. Conclusion: Method is simple, quick and good reproducible.

Pem gene is one of the homeobox gene. Unlike members of Hox gene family, Pem gene is unique located at the proximal end of the X chromosome and its expression has been detected in several immortalized and transformed cells, placenta, embryos and reproductive tissues. Numeral studies showed that its expression is controlled by hormone such as androgen. This review discussed the possible role of Pem in regulating genes involved in the differentiation and development of extraembryonic tissue, spermatogenesis and sperm maturation.

Objective To explore clinical characteristics and the perinatal effects of multiple systems in pre-pregnancy obesity patients with early-onset preeclampsia. Methods A total of 111 cases with early-onset preeclampsia in our Inpatient Obstetric Department were reviewed in this study. According to pre-pregnancy body mass index (BMI), patients were divided into normal before pregnancy group (BMI<28 kg/m2, n=56) and obese before pregnancy group (BMI≥28 kg/m2, n=55) . The body weight, BMI, weight gain during pregnancy, risk factors, clinical features, and clinical features of neonatal index were compared between two groups. The correlation between maternal and neonatal indexes and glucose and lipid indexes were compared. Results Obese before pregnancy was more prone to blood concentration and disorders of lipid metabolism. The serum levels of lipid, glycosylated hemoglobin (HbA1c), hematocrit, platelet and fibrinogen were significantly higher in obese before pregnancy group than than those of normal group. The induced neonatal acidosis and other organ damage, newborn Apgar score, pH, glucose were significantly lower in obese before pregnancy group than those of normal group. The residual alkali (BE), lactic acid (LAC), creatine kinase (CK) and creatine kinase (CKMB) were higher in obese before pregnancy group than those of normal before pregnancy group. Correlation analysis showed that maternal low density lipoprotein cholesterol (LDL-C), total cholesterol (TC), three acyl glycerol (TG) and HbA 1c were negatively correlated with neonatal Apgar score and pH, and which were positively correlated with neonatal LAC and CKMB. Both differences were significant (P<0.05 or P<0.01). Conclusion The dyslipidaemia in early onset preeclampsia in obesity patients before pregnancy is closely related with maternal and perinatal outcomes.

Objective To explore the IL-8 and ICAM-1 in premature infants with cerebral white matter damage (CWMD) and their correlations with prognosis. Methods One hundred and two cases of CWMD were selected from March 2009 to June 2012 as experimental group and 42 cases of normal preterm children were selected as control group. Serum IL-8 and ICAM-1 levels were measured during 48-72 h after birth. Motor development index (psychomotor developmental index, PDI) and mental development index (mental development index, MDI) were evaluated by Bayley scale. The correlations of IL-8 and ICAM-1 levels with prognosis were analyzed. Results Serum IL-8 and ICAM-1 levels in the experimental group were signiifcantly higher than those in the control group (P<0.05). MDI and PDI scores in the experimental group were signiifcantly lower than those in the control group (P<0.05). There were negative correlations of serum IL-8 and ICAM-1 levels with MDI (r=-0.64, P<0.05;r=-0.66, P<0.05)+and PDI (r=-0.70, P<0.05;r=-0.71, P<0.05). Conclusions Serum IL-8 and ICAM-1 levels in infants with CWMD are signiifcantly increased, and are negatively correlated with MDI and PDI scores.

Objective: To explore the relationship between parental rearing behavior and personality disorder. Method: Descriptive study was carried out in one key-university in Beijing. Two thousand two hundred and five freshmen were investigated with Personality Diagnostic Questionnaire-Revised (PDQ-R) and EMBU scale. Fifty-five personality disorder cases were diagnosed by International Personality Disorder Examination (IPDE). Results: The scores of emotional warmth, over-protection, and favoring subject of the maternal were higher than those of the paternal. Parental scores of EMBU factors were highly correlative. The scores of rejection, overprotection, favoring subject in personality disorder cases were significantly higher than those in control group, while the score of emotional warmth was significantly lower. Conclusion: Inappropriate parental rearing behavior is a risk factor of personality disorder.

Objective To construct recombinant adenovirus carrying the gene of human somatostatin receptor subtype 2(SSTR2),to investigate its effect on growth of human pancreatic cancer xenografts in nude mice,and to further elucidate the underlying mechanisms.Methods The recombinant adenovirus coding for human SSTR2(Ad-SSTR2)and reporter gene LacZ(Ad-LacZ)were generated by site-specific recombination from Jun 1,2004 to Dec 10,2005.Human pancreatic cancer cell line was implanted subcutaneously into nude mice;normal saline(control group),Ad-LacZ(reporter gene control group)and Ad-SSTR2(experimental group)were injected into pancreatic cancer xenografts,respectively.The tumor volume and weight were measured.RTPCR and Western blot were used to determine the expression of SSTR2 after pancreatic cancer xenografts were infected with Ad-SSTR2.The expression level of ERK2 and ras proteins were assessed by Western blot.Results The virus titer of Ad-SSTR2 and Ad-LacZ was 6.0?1012pfu/L and 6.5?1012pfu/L,respectively.SSTR2 mRNA and protein were detected after Ad-SSTR2 infected pancreatic cancer xenografts.Growth of pancreatic cancer was significantly inhibited in the experimental group as compared with the control group.The growth inhibitory rate was 48.2%(P

Objective:To survey the periodontal status of patients with metabolic syndrome in a Beijing community,so as to evaluate the relationship between periodontal status and metabolic syndrome.Methods:152 metabolic syndrome(MS)patients,106 post-acute myocardial infarction patients,211 hypertension patients and 56 healthy subjects were involved.Mesial buccal and distal lingual sites per tooth were examined.The periodontal status including plaque index(PLI),bleeding index(BI),probing depth(PD)and attachment loss(AL)as well as missing teeth number were recorded.Results:In male patients,PLI,BI and PD in MS group were higher than that in the other three groups although the mean age and smoking percentage of MS group were the lowest(P

Objective To compare the clinical value of two kinds of imunoassay method including CLIA and ELISA in the detection of related antibody of patients with HCV.Methods 60 patients with HCV were chosen as study subjects,and the related antibodies of patients were detected by CLIA and ELISA.The positive rates of anti HCV antibody,anti-3E antibody,anti-GP210 antibody,anti-SP100 antibody,anti-PML antibody and anti-AMA -M2 antibody detected by CLIA and ELISA were compared.Results The positive rates of anti-HCV antibody detected by CLIA and ELISA were 83.33％ (50/60),98.33％ (59/60),respectively.The anti-HCV antibody detected by CLIA was significantly higher than by ELISA (x2 =4.73,P =0.03).The positive rates of anti-3E antibody,anti-GP210 antibody,anti-SP100 antibody,anti-PML antibody and anti-AMA-M2 antibody detected by ELISA were 11.67 ％ (7/60),6.67 ％ (4/60),11.67 ％ (7/60),10.00 ％ (6/60),11.67 ％ (7/60),respectively,those detected by CLIA were 73.33 ％ (44/60),50.00％ (30/60),38.33 ％ (23/60),58.33 ％ (35/60),80.00％(48/60),respectively.The positive rates of anti-3E antibody,anti-GP210 antibody,anti-SP1O0 antibody,anti PML antibody and anti-AMA-M2 antibody detected by CLIA were significantly higher than by ELISA(x2 =18.81,11.51,13.23,15.08,13.75,all P＜0.05).Concelusion Compared with ELISA,CLIA in the detection of related antibodies of patients with HCV can efficiently higher the positive rate of anti-HCV and autoantibody.

AIM To investigate the effects of taurine(TAU) and propafenone(PRO) combination on experimental arrhythmias and its influence on haemodynamics of anesthesized rats' heart. METHODS Rats were pretreated respectively with intravenous administration of saline, TAU, PRO, or TAU and PRO combination. Afterwards, the rats were intravenously administrated with BaCl 2 or aconitine to induce arrhythmia. The arrhythmia lasting time and incidence were observed and recorded on the lead Ⅱ ECG. The cumulative aconitine dosage to induce premature ventricular contraction(PVC), ventricular tachycardia(VT) and ventricular fibrillation(VF) were calculated respectively. The haemodynamic parameters such as LVSP, HR, +d p /d t max were recorded continuously in anesthetized rats pretreated with iv TAU, PRO or TAU and PRO combination. RESULT In BaCl 2 arrhythmia, the arrhythmia duration was shortened significantly; and the arrhythmia incidence was decreased in the combination group, as compared with the single same dosage drug groups. In aconitine arrhythmia, the cumulative aconitine dosages to induce PVC, VT and VF were increased significantly in the combination group. Haemodynamics parameters showed that PRO(2mg?kg -1 iv) significantly decreased LVSP and +LVd p /d t max , while these parameters were not significantly changed in the combination group with same dose of PRO. CONCLUSION TAU and PRO have synergism in antiarrhythmia in both BaCl 2 and aconitine induced arrhythmia; TAU may alleviate cardiac depression exerted by PRO.

Objective To study the changes of NO plasma levels in patient with ischemic stroke(IS) of different types.Methods Using HNO 3 deoxidize enzyme method, we examined the levels of plasma NO in 230 patients with cerebral infarction, 103 patients with lacular cerebral infarction and 169 normal controls.Results In cerebral infarction group, the level of plasma NO was significant higher than the two other groups ( P