1.Effect of Silver Sulfadiazine-impregnated Hydrocolloid Dressing on Wound Care of Nail Extraction
Fanhui MENG ; Yongkun WANG ; Jiaru WANG ; Yueqiang TIAN ; Minna YAN ; Xiaoqiu YANG
Chinese Journal of Nosocomiology 2006;0(03):-
OBJECTIVE To observe the effect of the silver sulfadiazine-impregnated hydrocolloid dressing on the pain of nail extraction wound during dressing change and the healing time of wound. METHODS Forty eight patients with nail extraction were randomly divided into two groups: in the study group,whose wound was covered with silver sulfadiazine-impregnated hydrocolloid dressing;in the control group,whose wound was applied vaseline gauze when the nail had been extracted and the wound was applied antibiotic gauze during dressing change.The pain scores of two groups were compared.Two groups were compared with healing time and the times of dressing change. RESULTS The pain scores in the study group were significantly lower than that of the control group.The healing time of wound and the times of dressing change in the study group were less than that of the control one((P
2.Construction of Attenuated Influenza Virus Vaccines of Vaccines of-2006-2007
Penghui YANG ; Yi YE ; Xinfu SHI ; Deyan LUO ; Yu ZHANG ; Yan YAN ; Pengfei ZHANG ; Yueqiang DUAN ; Li XING ; Xiufan LIU ; Xiliang WANG
Progress in Biochemistry and Biophysics 2008;35(3):312-319
Six gene segments,PB1,PB2,PA,NP,M and NS,were fully synthesized which derived from the master donor virus (MDV),cold-adapted(ca),temperature sensitive(ts),live attenuated influenza virus strain A/Ann Arbor/6/60(MDV-A).Meanwhile,five amino acid substitutions (PB1-391E,58lG,661T,PB2-265S,NP-34G) were artificially altered by human intervention.HA and NA fragments derived from the 2006-2007 circulating strain A/New Caledonia/20/99 (H1N1).Eight fragments were ligated with modified pAD3000 for rescue plasmid construction.Eiight transcription/expression plasmids were named as pMDV-A-PB2,pMDV-A-PB1,pMDV-A-PA,pMDV-A-NP,pMDV-A-M,pMDV-A-NS,pMDV-A-HA,pMDV-A-NA,respectively.The COS-l cells were co-transfected with eight plasmids representing 6 internal viral backbone of the strain A/AA/6/60 and two plasmids containing the CDNA of the HA and NA segments of the strain A/New Caledonia/20/99 (H1N1),the results showed that cold-adapted,attenuated reassortant influenza A virus Was rescued successfully.Titers of a reassorted influenza A virus in embryonated chicken eggs mnged from 1:29to l:210.The rescue system of six intemal genes used as backbone opens the way for further research on gene function and neotype vaccine candidate of cold-adapted,live attenuated human influenza virus.
3.Construction of Attenuated Influenza Virus Vaccines of 2006~2007
Penghui YANG ; Yi YE ; Xinfu SHI ; Deyan LUO ; Yu ZHANG ; Yan YAN ; Pengfei ZHANG ; Yueqiang DUAN ; Li XING ; Xiufan LIU ; Xiliang WANG
Progress in Biochemistry and Biophysics 2006;0(03):-
Six gene segments, PB1, PB2,PA, NP, M and NS, were fully synthesized which derived from the master donor virus(MDV), cold-adapted(ca),temperature sensitive(ts), live attenuated influenza virus strain A/Ann Arbor/6/60(MDV-A). Meanwhile, five amino acid substitutions (PB1-391E, 581G, 661T, PB2-265S, NP-34G) were artificially altered by human intervention. HA and NA fragments derived from the 2006~2007 circulating strain A/New Caledonia/20/99 (H1N1). Eight fragments were ligated with modified pAD3000 for rescue plasmid construction. Eight transcription/expression plasmids were named as pMDV-A-PB2, pMDV-A-PB1, pMDV-A-PA, pMDV-A-NP, pMDV-A-M, pMDV-A-NS, pMDV-A-HA, pMDV-A-NA, respectively. The COS-1 cells were co-transfected with eight plasmids representing 6 internal viral backbone of the strain A/AA/6/60 and two plasmids containing the cDNA of the HA and NA segments of the strain A/New Caledonia/20/99 (H1N1), the results showed that cold-adapted, attenuated reassortant influenza A virus was rescued successfully. Titers of a reassorted influenza A virus in embryonated chicken eggs ranged from 1∶29 to 1∶210. The rescue system of six internal genes used as backbone opens the way for further research on gene function and neotype vaccine candidate of cold-adapted, live attenuated human influenza virus.
4.Association between dietary selenium intake and hypertension in Zhejiang residents
Mengjie HE ; Danting SU ; Yan ZOU ; Lichun HUANG ; Dong ZHAO ; Wei WANG ; Yueqiang FANG ; Enshan HUANG ; Wei GU ; Ronghua ZHANG
Journal of Preventive Medicine 2019;31(1):5-9
Objective:
To investigate the association between dietary selenium intake and hypertension among Zhejiang residents .
Methods:
By multistage stratified random sampling method,four urban sites and two rural sites out of Zhejiang Province,four communities or villages out of each site,then 20 households out of each community or village were selected,and all the family members of the selected households were recruited as participants. The questionnaire of Chinese Health and Nutrition Survey was used to collect information about socio-demographic characteristics and dietary selenium intake. The blood pressure,blood lipid and other data were collected via physical examination. A multivariate logistic regression model was used to analyze the relationship between dietary selenium intake and hypertension .
Results:
A total of 1 222 participants with complete dietary selenium intake data were included for analysis. The number(%)of participants with selenium intake higher than the level of estimated average requirement(EAR),between the levels of EAR and recommanded nutrient intake (RNI),between the levels of RNI and upper intake(UI)and higher than the level of UI were 729 (59.66%),151(12.36%),341(27.91%)and 1(0.01%),respectively. There were 283 (30.53%)patients with hypertension out of 927 participants examined. The mean amount of selenium intake in patients with hypertention was(43.06±20.96)μg/d,which was significantly lower than(51.56±30.06) μg/d in non-hypertention participants(P<0.05). After adjusting for age,body mass index,total cholesterol,triglyceride and diabetes mellitus in the multivariate logistic regression model,dietary selenium intake significantly reduced the risk of hypertension(OR=0.985,95%CI:0.978-0.993) .
Conclusion
About 60% of residents in Zhejiang Province had lower dietary selenium intake than estimated average requirement. Higher selenium intake was associated with lower risk of hypertension.
5. The treatment of application of pedicled myocutaneous island flap of anterolateral thigh muscle for the suprapubic defect in adolescent with bladder exstrophy
Lihua GUO ; Qian ZHANG ; Lei WANG ; Ji LI ; Yueqiang YAN ; Ningning YANG ; Ning LIU ; Yingzhong FAN ; Jiaxiang WANG
Chinese Journal of Plastic Surgery 2018;34(12):1023-1026
Objective:
To explore the treatment effect of pedicled myocutaneous island flap of anterolateral thigh muscle on the suprapubic defect caused by bladder exstrophy in children.
Methods:
The clinical data of 3 adolescents with bladder exstrophy were analyzed. All 3 cases underwent one-stage Kelly procedure and received the treatment using pedicled myocutaneous island flap of anterolateral thigh muscle to repair the suprapubic defect. The bladder expansion was performed with ileal sarcoplasmic layer graft, and the Cohen ureteral reimplantation was performed as well, for 1 case 1, 1 year after the first operation. The others 2 patients didn′t receive the second operation.
Results:
All operations were successful. The femoral donor regions were directly closed. Postoperative follow-up period was 1-7 years. The myocutaneous island flaps were survived and grew well. All incisions of the femoral donor region were primary healing without scar hypertrophy. Urinary continence was achieved in 2 cases, and partially achieved in 1 case. Recurrent urinary tract infection, recurrent fever or abdominal pain were not observed in all patients.
Conclusions
The pedicled anterolateral thigh myocutaneous island flap is an alternative way to repair suprapubic defect caused by bladder exstrophy.
6.Study Feasibility of Multi-payment for Drugs for Rare Diseases ——Taking Gaucher’s Disease in Qingdao as Example
Yan YANG ; Jiangjiang HE ; Yueqiang WANG ; Qi KANG ; Shanlian HU
China Pharmacy 2019;30(19):2593-2597
OBJECTIVE: To investigate the feasibility of multi-payment for drugs for rare diseases and also provide reference for rare disease treatment and the formulation of related policy. METHODS: Taking Gaucher’s disease in Qingdao as an example, the relevant medical insurance policies and drug supply were analyzed; according to the sources of financing, the economic burden of treatment drugs for patients with Gaucher’s disease in Qingdao was estimated. Based on the average total cost of patients with Gaucher’s disease in Qingdao, the drug cost burden of patients with Gaucher’s disease in the whole country was simulated according to the relevant epidemiological survey data, and the problems of medical insurance model for rare diseases in Qingdao were analyzed. RESULTS & CONCLUSIONS: A series of medical insurance policies were formulated in Qingdao. The financing mode of medical insurance includes social funds (from Qingdao Charity Federation, drugs are provided by pharmaceutical manufacturers), medical insurance funds (used for personal self-payment assistance within the scope of medical insurance co-ordination) and civil assistance (used for assistance outside the scope of medical insurance co-ordination). Imidase is currently the only approved specific drug for Gaucher’s disease in China. The designated physician, treatment and drug-taken system is adopted in Qingdao. By the end of 2017, the annual drug consumption of 8 patients in Qingdao was 38-170 bottles, totaling 686 bottles. The annual cost of treatment ranged from 786 600 to 3 519 000 yuan, totaling 1 420 200 yuan. The self-paid expenses ranged from 9 800 to 197 400 yuan (the self-paid ratio ranged from 0.46% to 8.87%), totaling 661 400 yuan. The reimbursement cost by supplementary medical insurance was 509 800 to 1 789 800 yuan (accounting for 54.59% to 65.94%), totaling 8 577 800 yuan. Three patients received civil assistance, the amount of which ranged from 23 100 to 13 000 yuan (accounting for 1.89% to 4.18%), totaling 241 400 yuan. According to the relevant epidemiological survey data (the prevalence rate was 0.15 per 100 000), it is estimated that there are about 2 093 patients with Gaucher’s disease in China. Referring to Qingdao multi-payment model, it is estimated that the total cost of drugs for Gaucher's disease in China is about 3.715 billion yuan, charitable assistance can bear 1.238 billion yuan, while medical insurance expenditure is about 2.255 billion yuan, and individual self-payment is about 222 million yuan. From the point of view of drug cost burden, the multi-payment model in Qingdao has lightened the personal burden of patients’ has achieved significant results. There are problems in Qingdao’s multi-payment model, such as “medical insurance immigration”, low drug accessibility, drug price monopoly, three-level disease prevention needs to be strenghten, etc. The state or provinces and cities can refer to Qingdao model when formulating policies related to rare diseases. Great importance should be paid attention to the existing problems.
7. Retrospectively analysis of characteristics and risk factors of immune treatment-related hepatic adverse events in malignant tumor
Shuluan LI ; Xiaoping GAO ; Qianqi CHEN ; Xiaohong FU ; Yan ZHAO ; Jiangman DUAN ; Yueqiang TANG ; Jie SUN ; Junling LI ; Qiming ZHOU
Chinese Journal of Oncology 2020;42(1):50-54
Objective:
To explore the clinical features and risk factors of hepatic injury due to immune checkpoint inhibitors (CPI) therapy in malignant tumor.
Methods:
Data of 112 patients (64 men and 48 women) who received CPI between January 2016 and March 2019 in Chinese Academy of Medical Sciences and Peking Union Medical College Shenzhen Hospital, and Huazhong University of Science and Techology Union Shenzhen Hospital were retrospectively collected. The median age of these patients was 60 years.
Results:
Hepatic adverse events were observed in 30 patients out of 112 patients (26.8%). Among them, the incidence of grade 3-5 hepatic adverse events were 7.14% (8/112). The median time of hepatic adverse event occurrence was 3 weeks (2-30) after undergoing therapy. The results of univariate and multivariate analyses showed that liver cancer was attributed to the CPI induced hepatitis (