Hypocomplementemic urticarial vasculitis (HUV) is a rare autoimmune disorder characterized by recurrent urticarial eruptions, hypocomplementemia and systemic involvement. This disease is prone to misdiagnosis, and its systemic manifestations may include arthritis, abdominal pain, renal involvement, chronic obstructive pulmonary disease and so on. Herein, we report a case of HUV presenting with recurrent abdominal pain, generalized wheals and arthralgia. Laboratory tests revealed decreased complement levels, skin histopathology was consistent with leukocytoclastic vasculitis, and imaging examinations indicated spondyloarthritis, thus confirming the diagnosis of HUV. The patient initially received systemic glucocorticoids combined with colchicine, whereas recurrent rash and arthralgia persisted. Tofacitinib (a Janus kinase inhibitor) and methotrexate were subsequently added to the regimen, leading to remission of symptoms. This case suggests that tofacitinib may serve as a potential therapeutic alternative for patients with HUV who respond poorly to glucocorticoids.

Objective:To analyze the activity of lithium chloride (LiCl) against influenza virus A (H3N2) in human non-small cell lung cancer cells (A549).Methods:Different concentrations of LiCl were incubated with A549 cells, and the cytopathic effect (CPE) was observed after 24 hours, and the effect of LiCl on cell activity was determined by CCK-8 method. After H3N2 (MOI=1) infected A549 cells, different concentrations of LiCl were added and incubated for 24 hours, and the viral load was measured by real time/reverse transcription quantitative polymerase chain reaction (RT-qPCR), and the CPE was observed, and the viral titer was determined. Different concentrations of LiCl were incubated with A549 at 37 ℃ and 5% CO 2 for 2 hours, virus was added and incubated for 24 hours, and the viral load was determined by RT-qPCR. LiCl, H3N2 and A549 were incubated at 4 ℃ for 1 hour, 35 ℃, 5% CO 2 for 24 hours, and viral load was determined by RT-qPCR. H3N2 and A549 were incubated at 4 ℃for 1 hour, then different concentrations of LiCl were added, incubated at 35 ℃ with 5% CO 2 for 24 hours, and the viral load was determined by RT-qPCR. After H3N2 infected A549 cells, different concentrations of LiCl were added and incubated for 24 hours, and the viral RNA load and viral titer of the supernatant and cells were measured, respectively, and then the corresponding ratios of the supernatant and the cells were calculated. After H3N2 (MOI=10) and BV (MOI=1) infected A549 cells, different concentrations of LiCl were added for 24 h, and the viral load was determined by RT-qPCR. Results:When the concentration of LiCl was<50 mmol/L, the cell viability of A549>90%. Different concentrations of LiCl could significantly reduce the viral load of H3N2 ( P<0.000 1), and the CPE of the LiCl treatment group was more dose-dependent than that of the control group. LiCl did not inhibit viral replication by affecting the cell itself; Different concentrations of LiCl significantly inhibited the entry of H3N2 into A549 ( P<0.000 1), and also had a certain inhibitory effect on the adsorption of A549 cells ( P<0.1). LiCl did not affect the assembly and release of H3N2 ( P>0.05), and it was also found that LiCl had a broad spectrum of antiviral effects against multiple influenza virus strains ( P<0.000 1). Conclusions:LiCl may exert antiviral effect by inhibiting the adsorption and entry of H3N2 into A549 cells and the replication of H3N2 in A549 cells, which provides a data reference for the prevention and treatment of viral infection by LiCl.

【Objective】 To evaluate and analyze the impact of COVID-19 epidemic on inventory of red blood cells (RBCs)in local and municipal blood stations in China, and to provide reference for the management of public health emergencies. 【Methods】 Relevant data from 2018 to 2021 were collected, and the differences in the volume of qualified RBCs, the usage efficiency of inventory RBCs, the average daily distribution of RBCs,the blood distribution rate of RBCs prepared by 400 mL whole blood, the difference in the average storage days of RBCs at the time of distribution, the average daily inventory of RBCs and the time of the average daily inventory of RBCs to maintain the distribution in 24 local and municipal blood stations in China during the COVID-19 epidemic and non-epidemic periods were retrospectively analyzed. 【Results】 Compared with non-epidemic periods, the volume of qualified RBCs [(117 525.979 ±52 203.175)U] and the average daily distribution of RBCs [( 156. 468 ± 70. 186) U ] increased significantly, but the usage efficiency of inventory RBCs decreased(97.24%±0.51%) significantly (P<0.05).There was no significant difference in the blood distribution rate of RBCs prepared by 400 mL whole blood(73.88%±20.30%), the average storage days of RBCs distribution(13.040 ±3.486), the average daily stock quantity of RBCs[(2 280.542 ±1 446.538) U ] and the time of the average daily inventory of RBCs to maintain the distribution[(15.062 ±7.453) d] (P>0.5). 【Conclusion】 During the COVID-19 epidemic, the inventory management of RBCs operated well, the overall inventory remained relatively stable, the stock composition and storage period showed no significant change.

Objective:To evalute the accuracy and clinical outcome of a real-time navigation system for the placement of quad zygomatic implants.Methods:Twenty-four patients [9 males and 15 females, mean age was (50.8±14.7) years old], from January 2015 to December 2019, with 96 zygomatic implants placed under a real-time navigation system in Department of Second Dental Center and Department of Oral Implantology of Ninth People′s Hospital, Shanghai Jiaotong University School of Medicine were included in the study. The preoperative and the postoperative multislice CT or cone-beam CT were fused to measure and record the entry, exit and angle deviation between the planned and placed implants. The implants were divided into groups according to implant insertion approach (real-time navigation and free-hand), implant length (<47.5 mm and ≥47.5 mm) and implant position (proximal and distal implant). And the differences of implant accuracy were analyzed. The intraoperative and postoperative complications were also recorded. The implant survival rate was evaluated after 6 months follow-up. A P value<0.05 indicates statistical significance. Results:The mean entry, exit and angle deviation of zygomatic implants were (1.49±0.64) mm, [2.03(1.58, 2.40)] mm and (2.49°±1.12°), respectively. The average entry, exit and angle deviation of the navigation guided implant insertion group were (1.45±0.60) mm, (1.96±0.44) mm and (2.66±1.13°) respectively, while those of the free-hand group were (1.50±0.64) mm, (2.04±0.79) mm and (2.50°±1.13°) respectively. There was no significant difference between the two groups ( P>0.05). The average entry, exit and angle deviation of the group with length<47.5 mm were (1.42±0.60) mm, (2.13±0.60) mm and (2.61°±1.08°) respectively and those of the group with length ≥ 47.5 mm were (1.52±0.65) mm, (1.98±0.82) mm and (2.43°±1.14°) respectively. No significant difference was found between the two groups ( P>0.05). In proximal implant group, the average entry, exit and angle deviation were (1.55±0.69) mm, (2.05±0.92) mm and (2.48°±1.16 °) respectively while those of distal implant group were (1.43±0.57) mm, (2.01±0.57) mm and (2.49°±1.10°), respectively. No significant difference was detected between the two groups ( P>0.05). All zygomatic implants were placed uneventfully. There were no intra-operative complications, and post-operative reversible complications developed in 3 patients. Two zygomatic implants were lost and the overall zygomatic implant survival rate was 97.9% (94/96) within a follow-up of 6 months. Conclusions:Quad zygomatic implant placement can be achieved with high accuracy and predictable clinical outcome under guidance of a real-time navigation system.

Objective: This study aimed to reveal the potential clinical and biological functions of frizzled related protein (FRZB) mRNA expression in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Methods: We used the keyword “lung cancer” to search the data through Gene Expression Omnibus (GEO) database attached to NCBI(National Center of Biotechnology) and download the data of LUAD and LUSC from TCGA (The Cancer Genome Atlas) Database. A total of eight LUAD and six LUSC datasets were incorporated in this analysis. We defined cutoff value of FRZB using Cutoff Finder into the two groups to calculate hazard ratio (HR). Results: We found that high expression level of FRZB mRNA in tumor tissues was a positive prognostic factor for overall survival in LUAD [pooled HR（95%CI）=0.54（0.46-0.64）,P＜0.05 in univariate analysis; pooled HR（95%CI）=0.66（0.54-0.79）,P＜0.05 in multivariate analysis]. Interestingly, there was no similar results in LUSC [pooled HR（95%CI）=1.11（0.67-1.84）,P＞0.05 in univariate analysis; pooled HR（95%CI）=1.13（0.71-1.78）,P＞0.05 in multivariate analysis]. We also found that FRZB may inhibit WNT signal pathway by t-SNE and correlation analysis. By enrichment analysis, FRZB and its most correlated genes were involved in multiple immune-related pathways, such as complement and coagulation cascades, humoral immune response, etc. Conclusion High expression of FRZB mRNA in LUAD was associated with better prognosis of lung adenocarcinoma. These results suggest that FRZB may be used as a potential marker for favorable prognosis of lung adenocarcinoma.

Objective This study aimed to examine temporal trends in all cancer-eliminated life expectancy and potential years of life lost (PYLL) in Kunshan city, Jiangsu province, between 1981 and 2015. Methods Data were collected from the vital records of Kunshan city. Cancer-eliminated life expectancy and PYLL were calculated by sex,and the Chinese population from the year 2000 was used to calculate age-standardized PYLL. Estimated annual percentage changes (APC) and 95% confidence intervals (CI) were used to examine temporal trends in increased cancer-eliminated life expectancy. Results Between 1981 and 2015,there was a significant decrease in age-standardized PYLL among males (APC=-2.31%, 95% CI:-2.51% to-2.11%) and females (APC=-1.91%, 95% CI:-2.26% to-1.56%). However, an increase was found in cancer-eliminated life expectancy in males and females, from 3.23 to 4.72 years in males(APC=2.42%,95% CI:1.64% to 3.20%)and from 1.10 to 2.94 years in females(APC=3.91%, 95% CI: 1.78% to 6.04%). Conclusion An overall decrease in age-standardized PYLL was observed when all cancers were considered,and an increase in cancer-eliminated life expectancy was found, suggesting that premature deaths due to cancers were reduced gradually. However, the negative effects of cancer on the health of the whole population have not been fully alleviated.

Objective To explore the mortality trends of cerebrovascular diseases and to quantitatively estimate the influence of population factors on cerebrovascular disease mortality between 1993 to 2016 in Kunshan city, Jiangsu province. Methods Records of cerebrovascular disease deaths were obtained from a register of all causes of death. Crude death rate and age standardized rate (ASR) were calculated, and annual percentage change (APC) based on sex and age were estimated. Difference decomposition was used to estimate the contribution of demographic and non?demographic factors to the change in cerebrovascular disease mortality. Results Between 1993 and 2013, the ASR of cerebrovascular diseases declined significantly for both sexes (APC=-4.9%, 95% CI:-5.5% to-4.3%), males alone (APC=-4.8%, 95% CI:-5.5% to-4.1%), and females alone (APC=-5.5%, 95% CI:-5.7% to-4.3%). However, from the year 2013 to 2016, the ASR of cerebrovascular diseases increased for both sexes together (APC=7.5%, 95% CI: 3.8% to 11.2%) and men alone (APC=10.2%, 95% CI: 4.3% to 16.1%), but there was no significant change for women (APC=4.3%, 95% CI:-1.2% to 9.8%). The overall decrease in mortality due to cerebrovascular disease was mainly due to non?demographic changes; the proportions of its contribution in men, women, and both sexes were 238.78%, 241.09%, and 245.89%, respectively. Conclusion The mortality due to cerebrovascular disease decreased substantially in the past two decades, and this decline was hindered by some demographic factors. It is imperative to explore the causes of this decline to decrease the burden caused by cerebrovascular disease.

Objective To explore the temporal trend of cancer death rates in different age and the influencing factors in Kunshan,Jiangsu province,1981 to 2015.Methods Data were derived from cancer rcgistry and vital registration system.The Chinese age structure in 2000 was used to calculate age-standardized death rates (ASR),and annual percentage changes (APC) and 95％ confidence interval (Cl) were used to estimate the temporal trend of cancer death rates.Difference decomposition method was applied to analyze the contribution of demographic and non-demographic factors for the change of cancer mortality.Results Between 1981 and 2015,the age standardized all cancers death rate decreased from 162.49 to 93.74 per 100,000 (APC=-l.6％,95％ CI:-1.8％--1.5％).However,the ASR for those aged 70 years or above was stable over time (APC=0.2％,95％ CI:-0.2％-0.5％),whereas aged 30-69 years was decreased from 240.01 in 1981 to 93.28 in 2015 (APC=-2.8％,95％ CI:-3.0％--2.6％).In addition,the proportion of leading cancers were changed obviously.The proportion of lung cancer increased from 1981 to 2015,while gastric cancer,liver cancer,esophageal cancer and colorectal cancer decreased.Compared with the crude cancer mortality in 1993,the effect of the demographic and non-demographic factors to the increased death rate in 2015 were 308.93％ and-208.93％,respectively.Conclusion The ASR death rate of all cancers was decreasing,and the rate in those aged 30 to 69 years decreased significantly,whereas stable in those aged 70 years or above.The effect of demographic characteristics on cancer mortality was significantly greater than that of non-demographic characteristics.

The paper reported three young women who collapsed into sudden abnormal behavior and convulsions and fell into unconsciousness. After admission, they were found to suffer from ovarian teratomas with anti-NMDAR antibody positive in cerebrospinal fluid or serum. The patients were diagnosed as anti-NMDAR encephalitis associated with ovarian teratoma, and teratoma resection was performed.

Objective To explore the effect of low dose ionizing radiation on the thyroid of radiation workers in our country. Methods The literatures were searched of domestic and foreign journals from 1996 to 2017. A total of 15 studies involving 6332 participants were included. These studies were screened according to the inclusion and exclusion criteria. Three indicators of the thyroid function were analyzed in radiation workers, including triiodothyronine ( T3 ) , thyroxine ( T4 ) and thyroid stimulating hormone( TSH) . The Meta analysis was performed using Stata 14. 0 software. Results For radiation workers, the standardized mean difference (SMD) of T3 levels was -0. 32[95% CI ( -0. 61,-0. 04), P=0. 03?, lower than that of the control group. Univariate meta - regression analysis of sources of heterogeneity showed that only gender composition was related to heterogeneity of the study [ adjusted R-squared (Adj R2 ) =29. 99%, P =0. 03]. The SMD of T4 levels was -0. 49 [95%CI ( -0. 94,-0. 03), P=0. 04], lower than that of the control group. Only the gender composition was related to heterogeneity of the study[Adj R2 =26. 60%, P=0. 02]. However, the SMD of TSH levels was 1. 10 [95% CI (0. 32,1. 89), P=0. 006], higher than that of the control group. Conclusions The long term exposure of radiation workers to low dose ionizing radiation may lead to negative impact on thyroid. It is important to strengthen radiation protection of thyroid.