OBJECTIVE:To study the difference and implication of 6?-OHFC/FC ratio at different phases of chemother-apy for leukemia children.METHODS:Urine samples were used for determination of 6?-OHFC/FC by HPLC.RESULTS:6?-OHFC/FC ratio had marked differences at different phases of treatment for leukemia children.CONCLUSIONS:6?-OHFC/FC ratio is of certain value in providing reference for treatment of children with leukemia,prognostic evaluation and risk classification.

OBJECTIVE: To establish a HPLC method for the determination of plasma level of cefuroxime in experimental dogs. METHODS: 1. 0mL plasma samples were taken from experimental dogs at different time after intravenous injection of cefuroxime sodium 50mg? kg-1. Following pretreatment, the samples were subjected to determination on XDB-C18 chromatographic column. The mobile phase consisted of CH3CN-1‰ ( NH4) 2SO4 ( 12∶ 88) with a flow rate of 1. 0mL? min-1. The detection wavelength was 273nm and the column temperature was 25℃ . The quantification was performed by external standard method. RESULTS: Good linear relationship was achieved when the detection concentration of cefuroxime was within the range of 0. 5～ 250? g? ml-1( r=0. 999 5) . The average recovery of cefuroxime was 97. 76～ 116. 00% ( RSD

OBJECTIVE:To establish a HPLC method for determination of prodatin in Polygonum cuspidatum injection.M_ETHODS:The analytical column was Hypersil ODS column(100mm?2.1mm,5?m).The mobile phase consisted of methanol-water(25∶75).The flow rate was 1.0ml/min.The UV detection wavelength was 290nm.RESULTS:The linear range was 0.145～2.32?g/ml(r=0.9 999).The average recovery of prodatin was 98.8%(RSD=1.15%).CONCLUSION:The method is convenient,accurate and could be used for quality control in the production of Polygonum cuspidatum injection.

OBJECTIVE:To establish a HPLC assay for determining stability of5%aciclovir ointment.METHODS:A HPLC method was used for the content determination of aciclovir in the ointment.The stablity of aciclovir ointment under humidity,light and heat was studied.RESULTS:The regression equation of aciclovir with the concentration C to the peak heights A∶C=9.310?10 -3 A+0.159,r=0.9999(n=6);the linear confine was0.6～19.2?g/ml;recovery was98.51%;RSD was0.42%.The quality and content showed no significant changes under humidity and light.The labelling amount percentages of aciclovir were98.36%,98.47%and98.26%in the test at40℃,60℃and80℃respectively.CONCLUSION:The HPLC assay is proved to be sensitive,accurate and simple.The ointment preparation has good stability in the test at40℃and under high light.

Objective:To establish an UHPLC-MS/MS method to determine the contents of baicalin,scutellarin,wogonin,caffeic acid,ephedrine,belamcandin,irisflorentin and baicalein in Shegan mixtures. Methods:The chromatographic separation was performed on a ZORBAX SB-C18 column(2. 1 mm × 50 mm,1. 8 μm)with the mobile phase consisting of acetonitrile and water(0. 1% formic acid),the flow rate was 0.3 ml·min-1,and the column temperature was 35℃. Electrospray ionization source(ESI)was used with multiple reaction monitoring( MRM)combined with positive and negative scanning switch. üegative ion mode detection was used for caf-feic acid,belamcandin and scutellarin ,while positive ion mode detection was used for baicalin,wogonin,irisflorentin,baicalein and e-phedrine. Results:The quantification limit of baicalin,scutellarin,wogonin,caffeic acid,ephedrine,belamcandin,irisflorentin and ba-icalein was 1. 44 × 10-4 ,4. 20 × 10-3 ,2. 95 × 10-4 ,7. 80,4. 90 × 10-3 ,4. 6 × 10-2 ,3. 18 × 10-4 ng·ml-1 and 4. 85 ng·ml-1 . The detection limit was 4.32 ×10-5,1.3 ×10-3,8.84 ×10-5,0.77,2.90 ×10-4,3.33 ×10-4,9.5 ×10-5 ng·ml-1 and 1.46 ng· ml-1 ,respectively. The correlation coefficients( R2 )were all higher than 0. 992 3 within the linear ranges. Both intra- and inter-day RSD were less than 5%. The average recoveries of the eight components were within the range of 80%-120%. Conclusion:The method is simple,rapid,sensitive and accurate. It can be used to determine the contents of baicalin,scutellarin,wogonin,caffeic acid,ephed-rine,belamcandin,irisflorentin and baicalein in Shegan mixtures for the quality control.

AIMTo evaluate CYP3A activity of hum an liver microsomes by the ratio of lidocaine(LDC) to its metabolites. M ETHODSIn 1 g?L -1 microsomal protein lidocaine was incubated at 37 ℃ for 1 hour. Lidocaine, monoethylglycinexylidide(MEGX) and glycinexylidide(GX) were determined by HPLC. RESULTSThe linear regress equations fo r LDC, MEGX and GX were = 0 293 4 X- 0 005 661 (r= 0 9 99 7 )、= 0 791 3 X- 0 008 916 (r= 0 999 3 ) and = 0 679 9 X- 0 007 770 (r= 0 998 5 ). The best conditio n for the incubation test in vitro was 2 0 mg?L -1 LDC plus 1 0 g ?L -1 microsomal protein for 60 min. The mean (MEGX+GX)/LDC ratio was 3 2 8. CONCLUSIONThe (MEGX+GX)/LDC ratio can be used to evaluate C YP3A activity of human liver microsomes.

OBJECTIVE: To establish HPLC method for the determination of thioguanosine in human red blood cells. METHODS: The determination was performed on Hypersil ODS colum. The mobile phase consisted of methanol-water (10∶90) with detection wavelength at 342nm. 6-Thioguanine(6-TG)was generated from thioguanosine through heating and hydrolyzing. 6-TG was extracted into 0.1mol/L hydrochloric acid for sample injection assay with external reference method for the quantification. RESULTS: The linear range of 6-TG was 30～1 200pmol/8?108RBCs. Its optimum hydrolyzing time was 1 hour and optimal extraction pH value ranged from 11 to 12. The content of phenyl mercury acetate in the extraction solution was 1.3mmol/L. CONCLUSION: Under optimized conditions, HPLC method for the determination of thioguanosine is fast, accurate and sensitive.

OBJECTIVE: To establish HPLC method for the determination of plasma etoposide level in children with leukemia. METHODS: The determination was performed on column Hypersil ODS. The mobile phase was water-methanol (45∶55) and the wavelength for detection was 284nm. RESULTS: The intraday recovery ranged from 93.56 to 96.24% with SD ranged at 1.07%～2.63%, and the inter-day recovery ranged from 92.85% to 94.26% with SD ranged at 3.55%～5.89%. CONCLUSION: This method is simple and sensitive, showed a good specificity, and suitable for the determination of etoposide in clinic samples.

OBJECTIVE:To discuss the development strategy of hospital preparations in new situation METHODS:To analyse existing circumstance and problems confronting us in development of hospital preparations RESULTS & CONCLUSION:In order to promote the development of hospital preparations,we should reform our work in respect to transformation of production,adjustment of kinds,promotion of R & D of new preparations and establishment of regional hospital preparation center

OBJECTIVE:To establish a HPLC method for the determination of ferulic acid in pedo spleen energy activating Misture.METHODS:The determination was performed on Hypersil ODS column,the mobile phase consisted of1%acetic acid-methanol(70∶30)with a flow rate of0.8ml/min,the column temperature was26℃,the detection wavelength was320nm and the sample size was20?l.RESULTS:Good linear relationship with peak area score was achieved when the detection con?centration range of ferulaic acid was within a range of0.02～1.2mg/ml(r=0.9997),the average recovery was97.88%(RSD=1.19%).CONCLUSION:The method can be served as a quality control for pedo spleen energy activating Misture.