Objective:To investigate the effect of long-chain non-coding CDKN2B targeting miR-19 on the biological behavior of chronic myeloid leukemia cells and its mechanism.Methods: The expression of CDKN2B in different leukemia cells were detected by qPCR.Double luciferase reporter gene was used to detect the interaction between CDKN2B and miR-19.MTT proliferation assay and flow cytometry were used to detect the effect of CDKN2B on the proliferation and apoptosis of HL-60 cells.The changes of migration ability of leukemia HL-60 cells after overexpress of CDKN2B were detected by scratch test.The changes of invasion ability of leukemia HL-60 cells after silencing CDKN2B were detected by Transwell invasion assay.Scaling healing experiment and Transwell invasion assay were used to detect the effect of miR-19 on the migration and invasion of leukemia cells after silencing CDKN2B.The morphological changes of cytoskeleton microfilament microtubules after silencing CDKN2B were detected by phalloidin staining.Western blot was used to detect the expression of PI3K/AKT signaling pathway after silencing CDKN2B.Results: The expression level of CDKN2B was the lowest in leukemia cell HL-60.CDKN2B binds specifically to the 3′UTR of miR-19;overexpression of CDKN2B could inhibit the proliferation and enhance the apoptosis of leukemia HL-60 cells.Overexpression of CDKN2B can inhibit the invasion and migration of leukemia HL-60 cells.After overexpressed of CDKN2B,the cytoskeleton showed decreased pseudopodia and decreased exercise capacity.The expression of actin was down-regulated.The expression of PI3K/AKT pathway protein was down-regulated after overexpressed of CDKN2B.Conclusion: CDKN2B can target the regulation of miR-19 to regulate the biological behavior of leukemia cells.

Objective:To observe the clinical efficacy of combined acupuncture and scraping therapy for simple obesity and its effects on phlegm-dampness constitution.Methods:Seventy subjects with phlegm-dampness constitution were divided into treatment group and control group with a randomized digital table.Control group was given health education and dietary instructions,and treatment group was given acupuncture and scraping therapy besides health education and dietary instructions.After four-week treatments,the scores of obesity and phlegm-dampness constitution were recorded to assess the clinical efficacy.Results:There was a striking difierence in the total effective rate in treating obesity between the two groups (P＜0.01);combined acupuncture and scraping therapy can relieve the phlegm-dampness symptoms(P＜0.01);but the phlegm-dampness symptoms were not improved in the control group(P＞0.05).Conclusion:Combined acupuncture and scraping therapy is quite effective for simple obesity and in improving phlegm-dampness constitution of simple obesity people.

Aim To study the apoptosis effect of cucurbitacin Ⅱa on non-small cell lung cancer cell lines NCI-H460 and A549 and its underlying mechanism.Methods Cell viability was assessed by CCK-8 assay.The apoptosis effect and cell cycle arrest were detected by Flow cytometry.Western blot was employed to detect the related protein.Results The proliferation of lung cancer cell lines NCI-H460 and A549 was inhibited by CuⅡa, which showed cytotoxic activity with IC50 values of 224.9 nmol·L-1 and 108.3 nmol·L-1 against NCI-H460 and A549 respectively.CuⅡa induced the cells apoptosis and cell cycle arrest at G2/M phase.The results of Western blot showed CuⅡa inhibited the phosphorylation of STAT3 and Cofilin in a dose-dependent manner.Further, CuⅡa inhibited the phosphorylation of Aurora A, in line with the important characteristics of anti-tumor effect of Aurora A kinase inhibitor with blocking cells in the G2/M phase.Conclusion CuⅡa has obvious anti-tumor effect against non-small cell lung cancer, which suggests its value as a lead compound for lung cell carcinoma.

Objective:To analyze the clinical characteristics and risk factors of fungal infections secondary to severe acute pancreatitis (SAP), so as to provide experience for clinical diagnosis and treatment.Methods:From January 2013 to August 2020, at The First Affiliated Hospital of Chongqing Medical University, 48 SAP patients with secondary fungal infection (infection group) were enrolled. At the same period, 72 SAP patients without fungal infection (non-infection group) were selected as control group. The location of fungal infection, bacterial species distribution, anti-fungal treatment of the infection group, and the prognosis of the two groups were analyzed. Independent sample t test, Wilcoxon rank sum test and chi-square test were used for statistical analysis. The factors that may affect the secondary fungal infection of SAP were analyzed by binary logistic regression analysis in order to detect the independent risk factors of SAP with secondary fungal infection, and receiver operating characteristic curve analysis was performed to evaluate their value in predicting SAP with secondary fungal infection. Results:There were 74 fungal infection sites in the infection group, mainly respiratory infections (25.7%); 54 fungal strains were isolated and all of which were Candida infection, mainly Candida albicans (48.1%). In the infection group, 36 patients received antifungal therapy, among whom 20 patients (55.6%) died; the remaining 12 patients did not receive antifungal therapy, six of them died, in total 26 (54.2%) patients died. In the non-infection group there were nine patients (12.5%) died. There was a significant difference in mortality between the infection group and the non-infection group (54.2% vs. 12.5%, χ2=24.20, P<0.01). Compared with the non-infection group, the acute physiology and chronic health evaluation Ⅱ (APACHEⅡ) score at admission, time of fasting and drinking, time of urinary catheter catheterization and deep venous catheterization, types and duration of broad-spectrum antibiotics usage, hospital stay, time of invasive ventilator using and indwelling time of abdominal drainage tube were higher in the infection group ((16.92±5.70) point vs. (13.32±4.73) point, (16.38±6.87) d vs. (9.51±4.90) d, (15.65±9.68) d vs. (10.40±9.45) d, (19.48±10.43) d vs. (12.74±10.28) d, (4.13±1.02) type vs. (2.35±1.78) type, (30.54±12.94) d vs.(19.10±9.48) d, (36.10±26.27) d vs.(21.93±9.91) d, 6.00 d (0.00 d, 21.75 d) vs. 0.00 d(0.00 d, 7.00 d), 9.00 d (0.00 d, 18.00 d) vs. 0.00 d (0.00 d, 0.00 d)), and the differences were statistically significant ( t=-3.61, -6.56, -3.08, -3.82, -6.86, -5.06 and -3.95, Z=-2.71 and -4.19, all P<0.01). The results of binary logistic regression analysis showed that APACHEⅡ score at admission, time of fasting and drinking, and broad-spectrum antibiotic usage were independent risk factors of SAP with secondary fungal infection (odds ratio=1.181, 2.589 and 1.205, 95% confidence interval ( CI) 1.036 to 1.347, 1.409 to 4.757 and 1.060 to 1.370), and the differences were statistically significant (all P<0.05). The area under curve values of APACHEⅡ score, broad-spectrum antibiotics usage and time of fasting and drinking were 0.695 (95% CI 0.596 to 0.794), 0.853 (95% CI 0.784 to 0.923) and 0.907 (95% CI 0.798 to 0.923), respectively; and the cut-off values at 17.5 point, 3.5 types, and 11.5 d were most effective in predicting secondary fungal infection of SAP. Conclusions:Respiratory tract is the most common site for secondary fungal infections in SAP. Candida albicans infection is more common. SAP patients with APACHE Ⅱ score >17 point at admission, time of fasting and drinking >11 d and more than three kinds of broad-spectrum antibiotics application are prone to secondary fungal infections in the later stage.

Objective:The association between leptin receptor (LEPR) Q223R (Gln>Arg) gene polymorphism and systemic lupus erythematosus (SLE) remains controversial.In this study, a meta-analysis was used to comprehensively evaluate the association between LEPR Q223R gene polymorphism and SLE susceptibility.Methods:Case control studies on the relationship between LEPR Q223R gene polymorphism and SLE susceptibility were comprehensively searched by Medline (PubMed), Web of Science, CNKI, Wanfang digital journal full-text database, etc., and the search time was up to April 2020.The data of A/G allele frequency and AA/AG/GG genotype in SLE patients and healthy controls were extracted, the odds ratio ( OR) value and 95% confidence interval ( CI) were used as the combined effect-size indicators to analyze the correlation between allele, genotype and SLE risk.The heterogeneity among studies was analyzed quantitatively, and the publication bias was evaluated by Begg and Egger’s test. Results:A total of 7 case-control studies from 4 studies were retrieved.A total of 9 052 patients with SLE and 8 146 healthy controls were included in the meta-analysis.The results showed that there was no significant association between LEPR Q223R A/G gene polymorphism and SLE susceptibility, and the OR of A allele in LEPR Q223R gene locus associated with SLE risk was 1.03(95% CI: 0.92-1.14). The dominant (AA+ AG vs GG) and recessive (AA vs AG+ GG) models both suggested that LEPR Q223R A/G gene polymorphism was not associated with SLE, and the combined OR (95% CI) was 0.88(0.15-5.37) and 1.13(0.37-3.49), respectively.The results also showed that the distribution of LEPR Q223R genotype was different among different populations, and the inter-study heterogeneity was large. Conclusion:The existing evidence is insufficient to indicate that there is an association between LEPR Q223R A/G gene polymorphism and SLE susceptibility, which needs to be confirmed by further studies.