Objective To develop a solid-phase extraction and HPLC method for the determination of midazolam and its hydroxy metabolites in child plasma. Methods Blood plasma samples were collected twenty four hours after treated with midazolam by continuous infusion of 2μg·kg-1·min-1 in our intensive care unit, the concentration of midazolam and its metabolites in plasma was detected by solid-phase extraction and RP-HPLC. The separation was performed on ZORBAX Eclipse XDB～C18 column(4.6 ×250mm,5μ m) with 7.56mmol/L ammonium sulfate - acetonitrile as mobile phase. The flow rate was 1.0ml/min,the column temperature was 25℃ and the wavelength of UV detector was 254nm. Results The retention time of 4-hydroxymidazolam, 1'-hydroxymidazolam and midazolam was detected as 8.14,9.09 and 11.18 min. The average recoveries for midazolam and its metabolites was 98.88% ～100.01% ,and RSD ＜ 4%. 29 patients took part in the study, the average plasma concentration of midazolam, 1'-hydroxymidazolam and 4-hydroxymidazolam was 0.68,0.28 and 0.08mg/L. Conclusion It is suitable for convention monitoring of the midazolam and its hydroxy metabolites concentration in the plasma

OBJECTIVE:To upgrade the management of drug storehouse.METHODS:The framework,function and advantages of management information system in our hospital drug storehouse was introduced and analysed.RESULTS&CONCLUSION:The application and improvement of information system in management of drug storehouse helps to make a more regular and scientific process.

Objective To explore the diagnostic value of CT hypotonic colonography in carcinoma of colon.Methods A total of 55 cases who were diagnosed as ileus,abdomen mass or suspected colonic carcinoma clinically were undergone CT hypotonic clolonography in six years recently,in our hospital.Of them,CT data of 35 cases identified by pathology were analysed retrospectively.Results CT diagnosis was corresponded to the pathologic results in 34 cases .Of them,33 cases were colonic adenocarcinoma(1 case with intussusception of ileum-colon type),one case was abdominal multiple metastatic adenocarcinoma.One case with cecal inflammation and granulation tissues hyperplasia was misdiagnosed for colonic carcinoma by CT.21 of 35 cases were undergone colongraphy,of them,15 cases were diagnosed as colonic carcinoma,1 case was normal,colonographic failure occurred in one case and 4 cases were false negative.Conclusion Hypotonic water perfusion CT scan in the cases permitted under clinical condition(patient with ileus should be perfused with 3% meglumine diatrizoate) can display clearly the primary focus,the position and range of ileus,surrounding organ.It is more excellent than conventional CT and X-ray examination.

OBJECTIVE:To establish a scientific evidence for evaluation of individualized administration among children.METHODS:Hydrocortisone probe was used for determination of CYP3A activity in children with quantitative method.RESULTS:CYP3A activity in the health children volunteers showed Gaussian distribution in the form of natural logarithm,the average value was1.69?1.04,with the value of1.63?0.99in males and1.75?1.09in females.CONCLUSION:There is no significant difference in CYP3A activity between the two sexes.

Objective To evaluate the cost-effectiveness of budesonide/formoterol powder inhalation(BF)versus salmeterol/fluticasone powder inhalation(SF)for the treatment of persistent asthma in adolescents,and to provide economic evidence and reference for clinical medication and decision-making.Methods From perspective of Chinese healthcare,a Markov model was established based on a prospective cohort study conducted by Shanghai Fudan University Affiliated Minhang Hospital.The cycle length was 7 days,and the time horizon was 6 years.A discount rate of 5％per year was applied.Clinical parameters including asthma acute exacerbation,treatment costs,and health utility values were obtained from relevant literature.The primary outcomes were direct medical costs,quality-adjusted life-years(QALYs),and incremental cost-utility ratio(ICUR).Cost-utility analysis was performed using TreeAge Pro 2022.The one-way sensitivity analysis and probabilistic sensitivity analysis were conducted to validate the robustness of the basic analysis results.Results Compared with SF group,BF group contributed to an incremental QALYs of 0.002 5 and reduced the cost by 7 043.93 yuan,which implied that BF group was a dominating treatment.Results of one-way sensitivity analysis showed that SF price,BF price and Hazard ratio of asthma acute exacerbation(BF vs.SF)were the main sensitivity factors of model.Probabilistic sensitivity analysis demonstrated the probability of BF being cost-effective was about 80％within the willingness to pay threshold of 257 100 yuan/QALY.Conclusions It was found out in this study that BF was a more cost-effective option for the adolescents with persistent asthma compared with SF.