1.Research progress in screening and applications of calcium-activated chloride ion channel modulators in Anoctamin family
Mingda WU ; Qiyuan HONG ; Yuejiao LAN ; Lan YAO ; Shiting XI ; Xueying LIU ; Juntao GAO ; Kai ZHENG ; Feng HAO
Chinese Journal of Pharmacology and Toxicology 2024;38(6):445-454
Calcium-activated chloride channels(CaCCs)are a class of channel proteins that trans-port chloride ions activated by intracellular calcium,which play a crucial role in regulating membrane potential,intracellular calcium balance,and cell excitability,particularly in neurons and muscle cells.In the Anoctamin(Ano)family,Ano1 is the most classic CaCC.Targeted modulators of Ano1 have poten-tial therapeutic effects against such diseases as cancer,cystic fibrosis,hypertension,diarrhea,and asthma.Since the discovery of Ano1 in 2008,several methods for screening CaCC-specific modulators have emerged including high-throughput primary screening of fluorescent proteins,electrophysiological patch clamp technique and virtual screening,and identification of small molecule modulators with diverse pharmacological effects.This paper summarizes the principles,advantages and disadvantages of the mainstream screening methods,and reviews the chemical structures and potential applications of Ano1-specific modulators discovered to date.
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