Objective To establish an HPLC fingerprint ofJinjing solid beverage,and provide experimental evidence of evaluating its quality.Methods Ten batches ofJinjing solid beverage were analyzed by HPLC under the gradient elution condition. A Kromasil C18 column(4.6 mm×250 mm, 5μm) was used, and the flow phase was acetonitrile-H2O(acidified to 0.1% with phosphoric acid) with gradient elution, and the detection wavelength was 327 nm, and the flow rate was 1.0 ml/min, and the column temperature was 35℃. The data were evaluated by the similarity evaluation software for TCM fingerprint.Results The HPLC fingerprint ofJinjing solid beverage were established. Twelve common peaks including chlorogenic acid were identified with similarity of more than 0.9.Conclusion HPLC method is a reliable, available and quick method, that provides a means for controlling and evaluating the quality ofJinjing solid beverage.

To extract sub-signal of heart period signal (HPS), a new statistical signal processing approach, namely independent component analysis (ICA) was addressed. Electrocardiosignal (ECS) was acquired from ten volunteers. ECS was sampled 8 minutes when the volunteer was in supine position, and then when the same volunteer was in erect position. HPS was extracted from ECS. According to time-delay, HPS was divided into five groups as mixed signals. Five signals were reconstructed into two groups by ICA. The rebuilt signals were transformed by Fourier transformation. One centralized in low frequency (called IC1); the other did in high frequency (called IC2). The power of IC1 was significantly increased (P<0.01) while that of IC2 showed no significant change (P>0.05), and the ratio of IC1 to total power also significantly increased with the change from supine position to erect position. Comparsion between the two postural results reveals that IC1 may express sympathetic activity, and IC2 represents parasympathetic activity. Sympathetic and parasympathetic nervous functions can be evaluated respectively and quantitatively by use of data and graphs from the two decomposed components.
Autonomic Nervous System ; physiology ; Electrocardiography ; Heart Rate ; physiology ; Humans ; Parasympathetic Nervous System ; physiology ; Signal Processing, Computer-Assisted

Objective Using hybridoma technique and screened hybridoma cell strains stably, efficiently secreted anti glycosylated hemoglobin monoclonal antibody to provide specific material for the development of glycosylated hemoglobin ELISA kit. Methods The immune antigen was prepared by maleimide method, multi-level immune mice by BALB/c,through cell culture fusion, screening of hybridoma cell culture medium HAT, ammonium sulfate salting out method and G protein chromatography. Monoclonal antibody subclasses were identified by monoclonal antibody subtype identi-fication Kit operation. Results Through cell fusion, screening and cloning culture, etc., the final selection screened 1 strain stably secreting specific antibody hybridoma cell line, named N5B4; cell culture supernatant liquid was 1:5000, ascites titer was 1:100 million; a standard curve to calculate the concentration in the sample human glycat ed hemoglobin was 99.2%. Conclusion After KET monoclonal antibody cell line to obtain a high specificity and high sen-sitivity of screening.