The vitamin B 12 Productivity of 14 marine bacterial strains isolated from the China Sea was determined microbiologically using Escherichia coli 113 3. Tests were made on the culturing media and culture conditions for Vitamin B 12 production. The results showed that the culturing medium in which the extract from soybean cake with seawater and some nutriments were added was suitable to recognize further some marine Vitamin B 12 producing bacterial strains. The medium with appropriate cobalt chloride and some salts added may be useful for Vitamin B 12 biosynthesis. The PH value of the fermentation for the testing strains was raised with the lapse of time for fermentation. A better Vitamin B 12 production may be obtained by keeping the fermentation liquid slightly basic till about 96h. No.2627 strain in all the strains tested showed well in the productivity. The mixed cultivation of some strains (including No.2627 strain) tested often has potentiality and superiority for Vitamin B 12 production.

Objective To investigate mutations of oncogene K-ras in colorectal cancer tissues and the relationship between mutations of K-ras and clinicopathological characteristics in colorectal cancer.Methods Specimens of 90 patients with colorectal cancer were detected K-ras gene by direct sequencing.Then satistical analysis were used to analyzed the associations between K-ras mutation status and clinicopathological characteristics in colorectal cancer.Results 31 cases were detected mutation in 90 cases of colorectal cancer specimens.The total mutation rate of K-ras was 34.4 ％.There were 21 eases of single mutation occurred in codon 12.One case had double mutation in codon 12.9 cases mutation were occurred in codon 13.There were significant difference between K-ras mutation rate and the location of tumor (P =0.042).The frequency of 12/13 codon mutation was no correlated with age,gender,tumor size,dukes stage,hepatic metastases,location of tumor.Conclusion The rate of K-ras mutation is correlated with the location of tumor.

The efficacy of different kinds of insulin analogue administered by multiple subcutaneous injection or continuous insulin infusion was compared. The two forms of administration all can well control blood glucose. Continuous subcutaneous injection has better compliance.

Objective To investigate the prevalence of elevated serum alanine aminotransferase ( ALT) and ALT-related dysmetabolism and risk factors. Methods A total of 12 789 adult participated,with no hepatitis B virus infection or alcohol over-intake, received health check-up, including blood pressure measurement, blood glucose and lipid profile test, liver function evaluation and ultrasonography. The subjects were then assigned into two groups according to the serum level of ALT. Metabolic composition was compared between the two groups. Results The prevalence of elevated ALT activity was 8.69%. The waist circumstance,body mass index (BMI) ,systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,total cholesterol (TC) ,triglyceride (TG) , low-density lipoprotein cholesterol (LDL-C) , urine acid (UA) , fasting blood glucose (FBG) ,HbAlc,total bilirubin (TBIL) ,-y-glutamyltransferase (GGT) ,alkaline phos-phatase ( ALP) of the elevated ALT group were significantly higher than those of the normal ALT group. However,age and high-density lipoprotein cholesterol ( HDL-C) in the elevated ALT group were lower than those in the normal ALT group ( P < 0. 05 ) . The occurrence of overweight, obesity, hyperlipidemia, impaired fasting glucose,diabetes, increased DBP, hyperuricacidemia and fatty liver in the elevated serum ALT group was significantly more than that in the normal ALT group ( P < 0. 05 ) . Multiple logistic regression analysis demonstrated that fatty liver, DBP, BMI.TC, HbAlc were risk factors of elevated serum ALT. Conclusion Non-alcoholic fatty liver disease, lipid metabolic disorders and obesity may be the main risk factors of elevated ALT.

Objective To compare the clinical efficacy of postoperative analgesia between intercostal nerve freezing and con‐trolled intravenous analgesia in patients of thoracic surgery .Methods 80 patients of thoracic surgery from January 2012 to June 2013 were randomly divided into two groups :Intercostal nerve cryotherapy group (frozen group n=40) and intravenous analgesia group(control group n=40) .Frozen group :the intercostal incision and down each one intercostal and chest tube placement of inter‐costal nerve roots were frozen before sternal closure ;control group :intravenous analgesia pump were used postoperative .According to VAS method to evaluate pain level and observe adverse reactions ,complications and analgesic drug usage of postoperative pa‐tients .Results The analgesic effect of frozen group was better than that of control group within five days after thoracotomy .Com‐pared with the control group ,the incidence of adverse reactions ,postoperative complications ,and analgesic drug usage was signifi‐cantly reduced in frozen group ,there was a significant difference between the two groups (P<0 .05) .Postoperative follow‐up dis‐play :intercostal nerve area in some patients may appear numbness ,dysesthesia ,etc .,but the above situation can return to normal gradually .Conclusion The analgesic effect of intercostal nerve cryotherapy for thoracotomy patients is excellent ,and with few side effects and good safety ,and it is worthy of promotion .

OBJECTIVE To make a new sterilizer to disinfect the instruments used for the root pulp treatment.METHODS After studying the SL sterilizer used in Japan,a new sterilizer was made.Studied how long the sterilizer could be used after putting through the electric.The effect of it was compared with the Eurouda sterilizer E6-18/24 by the method of culturing the bacteria.RESULTS 208.8 seconds after putting through the electric,the sterilizer could be used to sterilize the apparatus.And the effect of the new sterilizer was the same as the Eurouda sterilizer E6-18/24.CONCLUSIONS The effect of the sterilizer is testified,and the sterilizer could be used for the root pulp treatment instruments.

OBJECTIVE: To explore the clinical repair value of facial skin and tissue defect caused by tumour, trauma and infection with the axial flap. METHOD: The clinical data of 38 patients with facial skin and tissue defect were analyzed retrospectively. All of them were repaired by the axial flap. RESULT: The axial flap was alive in all patients, and all incision healed in the first stage. All patients had a satisfied result after the second stage of flap surgery. CONCLUSION The method of the axial flap in face can be clinically applied in the facial skin and tissue defects caused by tumour, trauma injury and infection. Because the axial flap cotained named blood vessel, the survival rate of it is high.
Dermatologic Surgical Procedures ; Face ; surgery ; Humans ; Reconstructive Surgical Procedures ; Retrospective Studies ; Skin Transplantation ; Surgical Flaps ; Wound Healing

Objective To investigate the epidermal growth factor receptor (EGFR),KRAS and BRAF mutations and their correlation with clinicopathological characteristics in non-small-cell lung cancer (NSCLC).Methods The mutations of exon 18,exon 19,exon 20 and exon 21 of the EGFR,codon 12,codon 13 of the KRAS and codon 600 of the BRAF gene in 143 cases of NSCLC were detected by gene sequencing.The relationship between the mutations and clinicopathological features was analyzed by SPSS 16.0.Results EGFR mutation was detected in 57 cases (39.9 ％),including 2 mutations in exon 18,25 in exon 19,3 in exon 20,24 in exon 21 and 3 multiple point mutations.KRAS mutation was found in 25 cases (17.5 ％),including 23 in codon 12 and 2 in codon 13.BRAF V600E mutation was detected only in 2 cases (1.4 ％).No patient harboring multiple EGFR,KRAS and BRAF mutations was found.EGFR mutation rate was related to gender,smoking history,histological types,differentiation and tumor size (P ＜ 0.05).However,no relationship was found among lymph node metastasis,pTNM stage and EGFR mutation (P ＞ 0.05).There was no association between KRAS mutation and clinicopathological features including gender,smoking history,histological types,differentiation,tumor size,lymph node metastasis and pTNM stage (P ＞ 0.05).Conclusions The frequency of EGFR mutation in NSCLC is high,and usually occurs in female,non-smokers,smaller tumors,better differentiation and adenocarcinomas.The frequency of KRAS mutation is not associated with the clinicolpathological features.The frequency of BRAF mutation is very low,and EGFR,KRAS and BRAF gene mutations do not occur at the same time.These results contribute to the target therapy of NSCLC.

Objective To apply hydride generation atomic fluorescence spectrophotometry (HG-AFS method) in urinary arsenic detection,and to provide a better,newer and more convenient detection method for quantitative analysis of urinary arsenic.Methods According to the Guide to Develop Biological Sample Inspection Method(WS/T 68-1996) and Guide for Establishing Occupational Health Standards-part 5:Determination Methods in Biological Materials (GB/T 210.5-2008),HG-AFS method was established to detect arsenic content in urine after modification of the method for sample pretreatment,and to verify the linear range of standard curve and linearity,detection limit,precision,accuracy,stability of the sample,and to compare the experimental results of HG-AFS method with those of standard methods of WS/T 28-1996 and Determination of Arsenic in Urine by Cyanide Generation Atomic Fluorescence Method (WS/T 474-2015).Results The HG-AFS method linear range was from 0-100 μg/L,the correlation coefficient r =0.999 9,the detection limit was 0.07 μg/L,the precision was 1.96％-3.97％,and the recovery rate was 95.1％-105.0％.There was no statistical significance between HG-AFS method,the standard of WS/T 28-1996 or WS/T 474-2015 methods (t =1.539,0.353,all P ＞ 0.05).Conclusion The new method is superior to the current detection method owing to its low detection limit,high precision,good accuracy,and wide linear range.

Objective To explore dyslipidemia in the people in the part of enterprises in Beijing.Methods Total cholesterol, triglyceride, high-density lipoprotein cholesterol, etc., in blood were detected in the physical examination of 12908 subjects in enterprises in Beijing. Dyslipidemia was analysed for the distribution of gender and the trend of age.Results The findings showed that the occurrence of hypercholesteremia is 21.3% with 20.3% for male vs 23.0% for female; hypertriacylglycerolemia is 30.4% with 36.19% for male vs 20.49% for female; and low level of high-density lipoprotein is 6.5% with 9.4% for male vs 1.6% for female. The trend of dyslipidemia increased with age. The subjects with coronary artery disease and related high risk diseases, who need medicine care, occupied 80.6% of the subjects with dyslipidemia.Conclusion Dyslipidemia increases with age and so regular detection of lipid is necessary especially for the subjects with coronary artery disease and related high risk diseases.