Objective :Evening primrose oil contains abundance of Essentia polyunsaturated fatty acids and ?-linolenic acid is the mostly important,but the property of ?-linolenic acid is unstable and easily oxidized,in this experiment the ?-linolenic acid was made into liposome so as to make it stable and more effective,orthogonal test was used to choose the Best Conditions of preparing the liposome of ?-linolenic acid.Methods:The best proportion of phospholipid and evening primrose oil is 0.2:0.1,dissolved in 20 ml chloroform and 40 ml phosphate buffer solution,ultrasonic treatment for 10min.Results:the?-linolenic acid entrapment efficiency was more than 99% and the permeability is low.Conclusion:The method is simple,accurate and reliable and can be used for the new drug development and production.

Aim To study the influence of PD on the activity of PKC(protein kinase C) of VSMC during ischemia and hypoxia.Methods The hypoxia model was made by drawing off the oxygen of an enclosed acryl glass box and ischemia model was made by low sugar culture medium. Then the activity of PKC was measured by ?-scintillation counting instrument.Results It was found that the activity of PKC of cytoplasm in VSMC in ischemia and hypoxia situation increased (vs normal group P0.05 ).Conclusion PD can reverse the PKC activities induced by ischemia and hypoxia. It may be one of the mechanisms of PD to have antishock effect.

[Objective] To summarize Professor WEI Yuegang's experience in treating Yangyu Hanning acne.[Methods]Through collecting medical record from outpatient clinics, Prof. WEI Yuegang's understanding of Yangyu Hanning acne and experience of Yangyu Hanning acne syndrome differentiation and treatment were analyzed and summarized.[Results]Prof. WEI Yuegang believes that the pathogenesis of Yangyu Hanning acne is Yang deficienay and Yang depression, Yang is deficient in the body, and the cold dampness blocks meridians, dysfunction of Shaoyang, Yang deficiency and Yang depression influence each other, Yang cannot supply and distribute, Yin cannot moisten, affter a long time it leads to heat in the upper and cold in the lower, or cold heat complex. In the treatment, we should give priority to warming yang and give consideration to the solution of depression, Prof. WEI Yuegang added and subtract on the basis of the Shenqi Pill and Xiaochaihu Decoction, and formulated Wenyang Jieyu Xiaocuo Decoction for treating the disease. Wenyang Jieyu Xiaocuo Decoction can both warm Yang and dispel cold and resolve Qi stagnation in the provided medical record, so the case achieved good results. [Conclusion]Prof. WEI Yuegang's treatment experience of Yangyu Hanning acne has satisfactory clinical efficacy and is worth to use for reference and promotion.