Objective To investigate the effect of using root cause analysis(RCA)on the improvement of disinfection and isolation.Methods A RCA group was established to find out the problems in disinfection and isolation.Then the related personnel were organized to study relevant knowledge,quality control was performed and disinfection management system was strictly executed. The managements of ultraviolet lamp,hand hygiene,use of disinfectants as well as the biological tests on air and hand were all compared between pre-and post-implementation of RCA.Results After implementing RCA,the use of ultraviolet lamp disinfection and hand hygiene were both improved than before implication(P < 0.05).But use of disinfectant for disinfection before the RCA implementation was not significantly better than that after the implementation(P>0.05).Conclusion The implementation of RCA may notably improve the quality of disinfection and isolation.