Objective:To study the effects of agonistic anti gp130 monoclonal antibody B S12 on the differentiation, maturation and function of dendritic cells (DC).Methods:Monocytes isolated from human peripheral blood were cultured with GM CSF plus IL 4, and differentiated into immature DC. The phenotype of DC was analyzed by cytometry after the addition of B S12 antibody to the culture of immature DC. In addition, the abilities for DC to uptake antigen, secrete IL 12, initiate the mixed lymphocyte reaction and chemoattract T cells were tested. The effects of agonistic anti CD40 monoclonal antibody 5C11 on the differentiation, maturation and function of DC were simutaneously compared with those of B S12 antibody.Results:Agonistic anti gp130 monoclonal antibody B S12 had DC to up regulate the expression of CD1a, costimulatory molecules CD80 and CD86 as well as CD83, which is special marker for mature DC, down regulate the expression of CD14. Moreover, B S12 antibody decrease the up take of antigen by DC, enhance the abilities for DC to secrete IL 12, initiate the mixed lymphocyte reaction and chemoattract T cells. The comparison of roles of B S12 and 5C11 antibodies in DC showed that 5C11 was more effective than B S12.Conclusion:The direct stimulation of gp130 on immature DC by B S12 antibody could induce immature DC to differentiate into mature DC.

Objective:To explore the effects of father-feeding attitudes on feeding status in preterm infants at discharge, and the role of father support breastfeeding self-efficacy, in order to provide a certain reference and basis for the intervention to improve the breastfeeding rate of premature infants.Methods:This was a cross-sectional study. A total of 402 premature infants' fathers were selected from 3 ClassⅢ Grade A hospitals from Zhejiang Province by convenience sampling method from July to November 2020. The basic data questionnaire, Iowa Infant Feeding Attitude Scale and Father Support Breastfeeding Self-Efficacy Scale-Short Form were used in the investigation. A total of 402 questionnaires were distributed, and 376 valid questionnaires were recovered. The recovery rate of effective questionnaires was 93.5%.Results:The score of father-feeding attitude of the 376 premature infants' fathers was (49.80±9.26) , and the score of support breastfeeding self-efficacy was (43.23±8.52) . Father's self-efficacy of breastfeeding support played a mediating role between feeding attitude and feeding status of premature infants at discharge, and the mediating effect accounted for 33.3% of the total effect.Conclusions:The father's feeding attitude can affect the feeding status of premature infants at discharge by influencing their self-efficacy of supporting breastfeeding. Medical staff should help premature fathers pay attention to breastfeeding, adopt practical and diverse health education methods to help premature fathers gradually master specific support behaviors and enhance their self-efficacy in supporting breastfeeding, and ultimately improve the rate of breastfeeding in premature infants.