Objective To investigate the status of quality of life and mental health among nurses in ClassⅢ Grade A hospitals in Beijing and to explore the correlation between them and analyze its main influencing factors.Methods From March to August 2017, the MOS Item Short From Health Survey-36 (SF-36) and Symptom Checklist-90 (SCL-90) were used to investigate 480 nurses from four ClassⅢ Grade A hospitals in Beijing. The Pearson correlation analysis and canonical correlation analysis were used to analyze the correlation between the two variables from multiple perspectives to determine the major factors influencing the correlation between them.Results Among 472 nurses of ClassⅢ Grade A hospitals in Beijing recruited in this research, the mean of total score of SF-36 was (66.02±14.26) with (56.76±21.01) for the lowest score in general health. The mean of total score of SCL-90 was (1.69±0.69) with 46.40％ for the positive rate of psychological problems. Pearson correlation analysis showed that the total score of SF-36 was negatively correlated with the total score of SCL-90 (r=-0.712,P<0.01), and canonical correlation analysis revealed that its major influencing factors included the dimensions in general health, mental health and depression. Conclusions Nurses of ClassⅢ Grade A hospitals of Beijing are with a low level of life quality and poor psychologic status with a negative correlation between them. Related functional departments should improve nurses' life quality and mental health from two aspects in physiology and psychology.