OBJECTIVE:To optimize drug for injection dispensing model in order to supply reference for popularity of automatic injection drug dispensing system(AIDDS) in inpatient dispensary.METHODS:Two kinds of drug dispensing model were adopted in five departments.In 10 working days before and after application of AIDDS,the time of drug dispensing flow needed was recorded.The time of every drug dispensing step needed for single patient was recorded and analyzed to evaluate the benefit of system.RESULTS:After application of AIDDS,the drug dispensing time for single patient reduced from 1.5～2.1 min to 0.45～0.80 min.The drug dispensing efficiency was improved significantly,the extent of which was in positive correlation to amount of patient demanding drug dispensing.CONCLUSION:The application of AIDDS speedups drug for injection dispensing,shortens dispensing time and saves human resource.

Objective To investigate whether intensive atorvastatin treatment in patients after percutaneous coro-nary intervention ( PCI) could decrease the effect of contrast medium on kidney function and the incidence of con-trast-induced acute kidney injury( CI-AKI) . Methods A total of 128 patients with PCI were randomly divided into two groups:the enhanced treatment group (n=64) and the control group(n=64). The enhanced treatment group received 80 mg atorvastatin at 12~24 h before PCI and 24,48 h after PCI. The control group was given 20 mg ator-vastatin respectively before and after PCI. The primary end point was the incidence of CI-AKI. Serum creatinine (Scr), cystatin C, glomerular filtration rate(eGFR), urinary albumin and urinary β-2 microglobulin levels were observed at 24 h before PCI and 24, 48, 72 h after PCI. Results In the enhanced treatment group 3. 1 % (n=2) of patients developed CI-AKI versus 4. 7 % (n=3) in the control group, without statistical difference (P=1.00). There was no significant difference between two groups in postoperative Scr, cystatin C, eGFR, urinary al-bumin, urinary β-2 microglobulin and creatine kinase(CK). Three days after the operation, alanine aminotrans-ferase ( ALT) elevated in two groups, and aspartate aminotransferase ( AST) increased in the enhanced treatment group (P<0. 05), but they were all in the normal range. Conclusion There has been no significant difference in decreasing the incidence of CI-AKI and the damage of contrast medium on renal function between the enhanced treatment group and the control group before PCI.

Objective To comprehensively understand the first aid skills for spinal cord injury of army members and improve their first aid skills through interventions.Methods A total of 2 200 troops members were selected within the army (Navy,Army and Air Forces).Intervention methods included questionnaire assessment,multimedia teaching and demonstration of first aid for spinal injuries.The total intervention time was 1 year,with once every four months.Results There distributed 2 200 copies of questionnaire before intervention and received 2 118 valid copies,with the total reclaim rate of 96.27％.A total of 2 118 copies of questionnaires were distributed after intervention and received 2 074 valid copies,with the total reclaim rate of 97.92％.Theoretical examination and skill test results of the army members were significantly improved after the intervention (all P ＜0.01).The general individual factors showed no effect on first aid of spinal cord injury before and after intervention.Before the intervention,the navy members had higher score than the land forces members and the air force members; however,no significant difference was found on the scores of different forces after the intervention.Conclusions The first aid skills for spinal cord injury of the army members has a big gap from the actual requirements.Improvement of first aid skills for spinal cord injury of the officers and soldiers can save the lives of themselves or comrades and hence is important in minimizing the combat attrition in future potential local high-tech wars.

Objective To identify the genetic locus for disseminated superficial actinic porokeratosis(DSAP).Methods Genome DNA was extracted from the whole blood of the family members of a pedigree of DSAP.Genotyping on chromosome12q that had been identified was performed by using7microsatellite mark-ers to scan the family members of DSAP and analysed with LINKAGE(5.1Version).Results A maximum2-point lod score of5.15with marker D12S79at a recombination fraction(?)=0.00was found.Conclusion Our study supports that DSAP gene localizes at the long arm of chromosome12,which was first reported in the literature.

Objective To evaluate the value of goat models in training and promoting key first aid skills of combat medics.Methods A total of 45 goats were anaesthetized with ketamine.Asphyxia models were created by wadding the goats' mouths and noses,pneumothorax models were established by piercing goats' chests,and massive hemorrhage models were built by piercing goats' limbs.Based on the above models,83 combat medics were trained to perform tracheotomy,tube thoracostomy,and artery suture.The differences in mastering these three skills before training,after training (immediately after training),and one year after training were recorded and compared.The effect of military service length and education background on the skill examination was evaluated before and after training.The recognition of the goat models from the combat medics was investigated.Results Before the training,success rates of performing tracheotomy,tube thoracostomy and artery suture were 39％,33％ and 25％,respectively.After the training,the corresponding parameters were 94％,86％ and 64％,respectively.One year after training,the success rates dropped to 66％,62％ and 43％,respectively.Compared with the status before training,the improvement of three skills was statistically significant after training (P ＜ 0.01).The decrease of three skills one year after training was statistically significant (P ＜ 0.01).The military service length had effects on the performance before training (P ＜ 0.05).And the military service length had no effect on the performance after the training (P ＞ 0.05).Education background had effects on the performance of surgical success rates before (P ＜ 0.01) and after training (P ＜ 0.05).According to the cross-sectional study,80％ of the military doctors chose the goat models as the first option for training.Conclusion Goat models are not only easy to be created but also are suitable for training the combat medics in key first aid skills.Such model is worthy of further application.

Objective To investigate the effect of transcranial direct current stimulation (tDCS) combined with motor imagery therapy on the function of upper limbs of stroke survivors.Methods Ninety-four stroke patients were randomized into a routine group (n=31),a motor imagery group (n=31),and a combination group (n =32).All the patients received basic routine rehabilitation therapy,while the routine group accepted traditional occupational therapy,the motor imagery group accepted motor imagery therapy,and the combination group accepted tDCS and motor imagery therapy.The upper limb function of the patients was assessed using Fugl-Meyer assessment (FMA) of upper extremities,the Hong Kong version of functional test for the hemiplegic upper extremity (FTHUE) and the modified Barthel Index (MBI) before and after 8-week treatment.Results The average FMA of the combination group and motor imagery group were (37.81 ± 12.80) and (31.39± 15.62),respectively,both significantly higher than that of the routine group (27.61± 14.90).The average FTHUE of the combination group and motor imagery group was both significantly higher than that of the routine group.Moreover,the average MBI of the combination group and motor imagery group were (57.78±7.73) and (51.87±9.31),respectively,both significantly higher than that of the routine group (45.19:t:9.07) (P＜0.05 for all).Conclusion tDCS combined with motor imagery therapy can significantly improve the upper limb function of stroke patients,significantly superior to tDCS or motor imagery therapy solely.

Objective To train the combat medics to learn key battle field operative technologies such as tracheotomy, thoracic close drainage, control of massive hemorrhage, etc by practicing the above skills on goats' traumatic models. Methods From 2012 to 2014 for consecutive 3 years, a group army trained the combat medics to learn how to treat war trauma first aid skills every year. 30 combat medics were randomly selected from 136 combat medics who had received goat model training to be tested. Goat model preparation methods: 35 healthy adult goats were anaesthetized with ketamine, then the suffocation models were reproduced by wadding the goat mouths and noses;pneumothorax models were replicated by cutting goat chest cavities; massive hemorrhagic models were produced by cutting goat femoral arteries by scissors. 136 combat medics were trained to perform tracheotomy, tube thoracostomy or thoracic close drainage, and hemorrhagic control by above models. The differences in mastering these skills before training, immediately after training, and one year after training were recorded and compared, and the factors influencing the combat medics training grade were analyzed. Results At the end of the training, the 30 combat medics' successful rates of performing cricothyrotomy or tracheotomy, tube thoracostomy, hemorrhage control were significantly higher than those before the training [respectively was 63.3% (19/30) vs. 10.0% (3/30), 66.7% (20/30) vs. 13.3% (4/30), 86.7% (26/30) vs. 53.3% (16/30), all P < 0.05]. After 1 year of training, the success rates of tracheotomy and thoracic close drainage were 33.3% (9/27) and 37.0% (10/27) respectively, which were significantly lower than those immediately after the end of training; the success rate of hemostasis after femoral artery rupture was 70.4% (19/27), which was lower than that at the end of training, but the difference was not statistically significant (P > 0.05). The education level had effect on the combat medics' performance after training. The success rate of combat medics with higher or above higher education was significantly higher than that of them with high school and below [88.9% (24/27) vs. 65.1% (41/63), P < 0.05]. Cross-sectional survey result showed that in 107 combat medics simultaneously received multimedia teaching, high analogue simulation human model teaching and animal model teaching, 85 combat medics (79.4%) chose the goat models as the first option for training. Conclusion By performing battle field key first aid techniques on goat trauma models, the combat medics' skills can be obviously elevated, they approve this animal model training as the first option, but repetition of the training is necessary to maintain the skills long lasting.

Objective:To explore the effect of combining motor imagery therapy (MIT) with kinesio taping in rehabilitating the upper limb motor function of stroke survivors.Methods:Ninety-two stroke survivors were randomized into a control group ( n=31), an MIT group ( n=31), and a combination group ( n=30). All were given 40 minutes of basic rehabilitation therapy daily, while the MIT group received additional MIT therapy, and the combination group received kinesio taping with the MIT therapy. The taping was applied according to a patient′s condition and changed every other day. The MIT was conducted twice a day. The experiment lasted 8 weeks, six days a week. Before and after the 8 weeks, the upper limb functioning, ability in the activities of daily living and muscle tension of each subject were assessed using the Fugl-Meyer assessment for the upper extremities (FMA-UE), the Hong Kong version of the functional test for a hemiplegic upper extremity (FTHUE-HK), the modified Barthel index (MBI) and the modified Ashworth scale (MAS). Results:The average post-treatment FMA-UE and MBI scores of the combination group were significantly higher than those of the MIT group, and both were significantly higher than the control group′s averages. The average FTHUE-HK grading of the combination group and MIT group after the treatment was significantly higher than in the control group, with that of the combination group significantly superior to the MIT group′s average. After the intervention the average MAS rating of the combination group was significantly lower than that of the control group.Conclusion:MIT combined with Kinesio taping can significantly improve the upper limb motor functioning of stroke survivors, and significantly reduce their abnormal muscle tone compared to traditional treatments.

OBJECTIVETo disclose the molecular genetic mechanism of type 2 diabetes mellitus and to determine chromosomal location of type 2 diabetes mellitus gene.

METHODSGenome-wide screening and genotyping were conducted in a family of type 2 diabetes mellitus, and linkage analysis by LINKAGE and GENEHUNTER package was used to determine the potential chromosomal location of the family of type 2 diabetes mellitus gene.

RESULTSEvidence of linkage was found at the long arm of chromosome 2. The maximum Lod score is 1.80 and non-parameter Lod score is 5.06.

CONCLUSIONThe type 2 diabetes mellitus gene of the family is located at 2q.

Adult ; Age of Onset ; Aged ; Aged, 80 and over ; Chromosome Mapping ; Chromosomes, Human, Pair 2 ; genetics ; Diabetes Mellitus, Type 2 ; genetics ; Family Health ; Female ; Genetic Linkage ; Genetic Predisposition to Disease ; genetics ; Genome, Human ; Humans ; Lod Score ; Male ; Microsatellite Repeats ; Middle Aged ; Pedigree

Objective: To understand the current status of BMI of the elderly and related factors in longevity areas in China, and provide scientific evidence for the control of BMI level in elderly population. Methods: Data used in this study were obtained from Healthy Aging and Biomarkers Cohort Study, a sub-cohort of the Chinese Longitudinal Healthy Longevity Survey. A total of 2 825 elderly in 8 longevity areas in China were surveyed and measured in 2017. The BMI levels of 2 217 elderly aged 65 years and older were calculated and in follow up. The ordered classification logistic regression model was used to analyze the influencd factors for the BMI in the elderly. Results: The BMI of the elderly in 8 longevity areas in China was (22.36±3.87) kg/m², and it was (22.76±3.58) kg/m² for males and (21.75±3.98) kg/m² for females. The BMI levels were normal in 1 165 elderly persons. The prevalence of underweight, overweight and obesity were 15.8%, 24.0% and 7.7%, respectively. Multivariate analysis showed that the main factors affecting the BMI of people under 100- years old were age (65-: OR=2.78, 95%CI: 1.87-4.15; 80-: OR=1.47, 95%CI: 1.00-2.17), smoking status (OR=0.46, 95%CI: 0.32-0.66), annual household income (<30 000 Yuan: OR=1.26, 95%CI: 1.07-1.47; 30 000-70 000 Yuan: OR=1.52, 95%CI: 1.12-1.86), and frequency of tea intake(OR=1.36, 95%CI: 1.01-1.71), while the factor in people aged ≥100 years was gender (OR=3.68, 95%CI: 1.32-10.36). Conclusions The prevalence of underweight, overweight and obesity were high in the elderly from longevity areas in China. It is necessary to pay attention to the trend of overweight and obesity due to smoking, higher annual household income and regular tea drinking in the elderly men.