The article analyzed the contemporary study of pattern recognition of tongue images in traditional Chinese medicine by computer technology from its key sectors. It regards that the methods of orthogonal design and evaluation of image quality should be introduced to get a high-quality and practicable condition. In the field of tongue image segmentation, multiple methods used together are expected to improve the effect of segmentation. A scientific and canonical standard of tongue diagnosis based on comprehensive experts' experience with Delphi evaluation method and multi-center experts diagnosis on internet are also very essential. The study of pattern recognition on tongue image is still concentrated on the color. Further study should be extended to achieve quantification. And a comprehensive diagnosis on pattern recognition of tongue image is the aim that should be focused on later.

With the further development of clinical modernization of traditional Chinese medicine (TCM), new request to tongue diagnosis for objectivity and quantification is raised. Many researchers have devoted themselves to this field and have achieved some accomplishments since 1950's. Especially in recent years, the revolution of information technique derived from computer industry brings the research new chances. The research history of the past five decades can be divided into three stages: (1) looking for micro-markers by newly-developed medical technique; (2) quantifying traditional markers by new technique; (3) further development based on information technique and corporation of multiple subjects. This article reviewed the three stages and described its prospect.

Objective To investigate the multiple factors correlated to femoral head necrosis in patients over 45 years old with femoral neck fractures after closed reduction and cannulated screws fixation.Methods Three hundred patients with femoral neck fracture were admitted from May 1999 to May 2004 and treated by closed reduction and internal fixation with cannulated screws.We analyzed the multiple factors:age,gender,Garden classification,follow-up time,procedure delay,quality of reduction,time of full-weight-loading,fixation removal, and preoperative traction.Multiple correlation factors were analyzed by SPSS10.0 statistic system.Results Complete case records were documented in 99 cases who were followed up for an average of 24.5 months(range,8 to 60 months).Femoral head necrosis occurred in 15 cases(15.2%).Time of femoral head necrosis was 8 to 50 months after operation.The combination of quality of reduction,preoperative traction,age,and age?fixation re- moval was found to have the most significant influence on femoral head necrosis after internal fixation for femoral neck fractures.Conclusions Good reduction is the most important factor correlated to the outcome of a femoral neck fracture.Age is linked to the risk of necrosis but it is relatively insignificant.Preoperative traction in wrong positions can increase the incidence of necrosis.Garden classification is important in predicting femoral head necrosis and is associated with quality of reduction,but it is not a risk one in the combination of multiple correlation factors that lead to the necrosis.

[Objective] To explore the mechanism of its formation of sublingual varix in patients with primary liver cancer(PLC) and from the vessels and haemodynamics so as to establish the basis for its future clinical study.[Methods] The related indexes of the haemodynamics vessels tongue and the portal system were measured by Doppler ultrasound.Then the mechanism of the formation of abnormal sublingual varix was explored from the local microcirculation of tongue,the morphology and haemodynamics of the tongue vessels and portal system.[Conclusion]The formation of abnormal sublingual varix was related to haemodynamics of the tongue,and the abnormal morphology and haemodynamics of portal system.The deep mechanism need to be further explored.

In order to investigate the role of Th17 cytokines in the pathogenesis of psoriasis, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of IL-17, IL-23 (p19/p40), and IL-6 in skin lesions and non-lesions of the patients with psoriasis and skin tissues of normal subjects. The results showed that the mRNA expression levels of IL-17, IL-23p19, IL-23p40 and IL-6 in psoriasis lesion were significantly higher than those of non-lesions (1.231 +/- 0.843 vs 1.003 +/- 0.044, 1.166 +/- 0.142 vs 0.765 +/- 0.133, 1.125 +/- 0.104 vs 0.730 +/- 0.103, 1.186 +/- 0.222 vs 0.976 +/- 0.122, respectively, all P < 0.05). Meanwhile, The expression levels of IL-17 mRNA, IL-23p19 mRNA, IL-23p40 mRNA and IL-6 mRNA were higher in non-lesions than those in normal skin tissues (1.003 +/- 0.044 vs 0.620 +/- 0.104, 0.765 +/- 0.133 vs 0.584 +/- 0.078, 0.730 +/- 0.103 vs 0.000 +/- 0.000, 0.976 +/- 0.122 vs 0.656 +/- 0.121, respectively, all P < 0.05). The overexpression of Th17 cytokines in the skin lesions of patients with psoriasis may indicate that Th17 cytokines play a very important role in the immunopathogenesis of psoriasis.

24 hours) and versus control group(

Objective To investigate the effects of propofol and sevoflurane on oxidative stess response induced by short period pure oxygen inhalation during general anesthesia.Methods Sixty ASA Ⅰ or Ⅱ patients aged 20-60 yr weighing 50-85 kg undergoing elective abdominal surgery under general anesthesia were randomly divided into 2 groups (n=30 each):group propofol (group P) and group sevoflurane (group S).Each group was further divided into 2 subgroups inhaling 40% O2 (P0.4,S0.4) and 100%O2(P1.0,S1.0) respectively during operation.Anesthesia was induced with propofol 1-2 mg/kg,midazolan 0.02 mg/kg and sufentanil 0.1-0.2 mg/kg.Tracheal intobation was facilitated with rocuronium 0.6-0.8 mg/kg.The patients were mechanically ventilated(VT 8 ml/kg,RR 12 bpm).PET CO2 was maintained at 35-40 mmHg.Anesthesia was maintained with in both groups.BIS was maintained at 40-60.Arterial blood samples were collected immediately before induction of anesthesia (baseline),at 2,4,6h after tracheal intubation(T1-3) and 24h after operation(T4) for determination of PaO2,serum 8-iso-PGF2α and MDA concentrations and SOD activity.PaO2/FiO2 was calculated.Results In subgroup S1.0 the serum 8-iso-PGF2α and MDA concentrations were significantly increased while serum SOD activity was significanfly decreased at T1-3 as compared with the baseline.Serum 8-iso-PGF2α and MDA concentrations were significantly higher while serum SOD activity and PaO2/FiO2 were significantly lower at T1-3 in subgroup S1.0 than in stress response induced by≤6h pure O2 inhalation but inhalation of 1.5%-3.0% sevoflurane can not.

Objective To assess the effect of repetitive transcranial magnetic stimulation (rTMS) at different frequencies on behavioral and psychological symptoms and cognitive function in patients with Alzheimer's disease.Methods Seventy-two patients were randomly divided into a high frequency group,a low frequency group and a control group (n =24 for each),and given 10 Hz,1 Hz or sham rTMS over the left and right dorsolateral prefrontal cortex at an intensity was 80％ of each patient's movement threshold.The stimulation was for 30 min a day,five days a week.During the 4 weeks of treatment,risperidone was used at a fixed dose of 1 mg per day for all patients.Before and after the treatment,all 3 groups were assessed using the behavioral pathology in Alzheimer's disease rating scale (BEHAVE-AD) and the mini-mental state examination (MMSE).Results Two weeks after the treatment,the average BEHAVE-AD score of the high frequency group had decreased significantly from (16.75±6.26) to (13.84± 6.10),significantly lower than the low frequency and the control groups' averages.Two weeks later,the average BEHAVE-AD scores of the 3 groups were significantly lower than before the treatment,but the decrease of the high frequency group was of significantly greater compared to the other 2 groups.Four weeks after the treatment the average MMSE score of the high frequency group was significantly better than before the treatment (P＜0.01),and better than the low frequency and the control group's averages.The effectiveness rates of the high frequency group,the low frequency group and the control group were 88％,58％ and 54％ respectively,all significantly different.Conclusion High frequency (10 Hz) rTMS combined with risperidone can improve the behavioral and psychological symptoms and cognitive function of AD patients,while low frequency (1Hz) rTMS does not have obvious effects.

Schistosomiasis is a kind of zoonosis with serious hazard,which is popular in many countries and regions in the world. One of the efforts for schistosomiasis prevent and control is developing new drugs and vaccines,and knowing the tran?scription regulation mechanism and the function of transcription factors will help us find the targets of new drugs and vaccines as soon as possible. This article reviews the progress of Schistosoma transcription factors and research methods.

Objective To observe the anti‐metastatic effect and mechanism of baicalin on the growth of HeLa cells was measured by MTT assay ,and cell migration baicalin on human cervical cancer HeLa cells .Methods The effects of baicalin on the proliferation and invasion of HeLa cells were analyzed by MTT method and Transwell assay .Moreover ,Real‐time PCR was used for investigating the expressions of MMP‐2 and MMP‐9 at the RNA level .Western blot was used for investigating the expressions of MMP‐2 ,MMP‐9 ,P38 and p‐P38 at the protein level .Results Baicalin could significantly inhibit the proliferation of HeLa cells in the dose‐dependent manner at the concentration above 60 μg/mL . Anti‐metastatic signaling induced by baicalin was characterized by down‐regulating the RNA and protein expressions of MMP‐2 and MMP‐9 ,and down‐regulating the phosphorylation level of P38 . Pre‐treatment of P38 signal pathway inhibitor could enhance the inhibitory effect of baicalin on the expressions of MMP‐2 and MMP‐9 .Conclusion These results indicate that baicalin‐induced anti‐metastatic effect involves the inhibition of MMP‐2 and MMP‐9 in HeLa cells through P38 signal pathway .