5.The Community Succession of Sarcosaphagous Insects on Pig Carcasses in Summer Indoor and Outdoor Environment in Shenzhen Area
Xiaojun YIN ; Mengyun MA ; Hui ZHOU ; Yue LAI ; Jiangfeng WANG
Journal of Forensic Medicine 2014;(3):172-177
Objective To explore the growing development and community succession of main sarcos-aphagous insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area and to estimate the postmortem interval (PMI). Methods From early May to August in 2013, in Forensic Med-ical Examination Center of Shenzhen Public Security Bureau, the main insect species and the decomposi-tion process were observed in two adult pig carcasses of simulative indoor and outdoor environment. The different decomposition stages and the community succession of insects were recorded. Results The indoor and outdoor pig carcasses showed skeleton 412.5 and 325 hours after death, respectively. The main species of flies on pig carcasses were Chrysomya megacephala, Chrysomya rufifacies and Chrysomya chani. The main species of beetles were Crecphilus maxillosus, Necrobia ruficollis, Saprinus splendens and Dermestes maculatu. The dominant species of flies in the outdoor pig carcasses obviously produced the second generations due to the effect of mass rainfall, nor in the indoor pig carcasses. Conclusion There are regular patterns on the community succession of insects on pig carcasses in summer indoor and out-door environment in Shenzhen area. The activity patterns of seven typical insects and their larva show important value for estimating PMI.
6.Protective effects of Cordyceps sinensis polysaccharide CPS-A on angiotensin Ⅱ-induced injury of liver L02 cells
Zhengqi ZHA ; Qiuli CHEN ; Yufeng WANG ; Hui ZHU ; Ying WANG ; Hongping YIN ; Long YUE
Journal of China Pharmaceutical University 2017;48(4):490-495
This study aimed at the molecular mechanism of Cordyceps sinensis polysaccharide-A(CPS-A) on angiotensin (Ang Ⅱ)-induced injury of L02 cells.The effect of AngⅡ and CPS-A on the proliferation of L02 cells was analyzed by MTT assay.PCR,Real-Time PCR and Western blot were also employed to determine the expression of IL-1β,AT1R,AT2R,NF-κB p65,TNFα and other inflammatory factors at mRNA and protein levels.The results showed that Ang Ⅱ and CPS-A could inhibit the proliferation of L02 cells by 1 × 10-5 mol/L and 200 μg/ mL,respectively.PCR,Real-Time PCR and Western blot showed that CPS-A could significantly down-regulate IL-1 β,TNF-α,NF-κB and AT1R.CPS-A has a good protective effect on AngⅡ-induced L02 cell injury.
7.The community succession of sarcosaphagous insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area.
Xiao-Jun YIN ; Meng-Yun MA ; Hui ZHOU ; Yue LAI ; Jiang-Feng WANG
Journal of Forensic Medicine 2014;30(3):172-177
OBJECTIVE:
To explore the growing development and community succession of main sarcosaphagous insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area and to estimate the postmortem interval (PMI).
METHODS:
From early May to August in 2013, in Forensic Medical Examination Center of Shenzhen Public Security Bureau, the main insect species and the decomposition process were observed in two adult pig carcasses of simulative indoor and outdoor environment. The different decomposition stages and the community succession of insects were recorded.
RESULTS:
The indoor and outdoor pig carcasses showed skeleton 412.5 and 325 hours after death, respectively. The main species of flies on pig carcasses were Chrysomya megacephala, Chrysomya rufifacies and Chrysomya chani. The main species of beetles were Crecphilus maxillosus, Necrobia ruficollis, Saprinus splendens and Dermestes maculatu. The dominant species of flies in the outdoor pig carcasses obviously produced the second generations due to the effect of mass rainfall, nor in the indoor pig carcasses.
CONCLUSION
There are regular patterns on the community succession of insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area. The activity patterns of seven typical insects and their larva show important value for estimating PMI.
Animals
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Autopsy
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Cadaver
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China
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Coleoptera
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Death
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Diptera
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Environment
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Insecta/growth & development*
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Larva
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Population Dynamics
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Postmortem Changes
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Seasons
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Swine
8.Effects of propyl gallate on serum inflammatory factors and protein expression of COX-2 and ICAM-1 in ischemic myocardium of rats with acute myocardial infarction.
Yue-Rong JIANG ; Hui-Jun YIN ; Ying LIU
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(10):921-924
OBJECTIVETo investigate the effects of Propyl Gallate (PrG) on serum inflammatory factors and protein expression of cyclooxygenase-2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) in ischemic myocardium of rats with acute myocardial infarction (AMI).
METHODSAMI model was induced by ligating the left anterior descending (LAD) branch of coronary artery in Wistar rats, and the perfect modeling was certified with ST segment elevation by standard limb lead II of electrocardiogram. Rats were randomly divided into 6 groups: Group A of normal rats, Group B of rats through sham operation, Group C of AMI model rats, Group D of model rats treated with high dose PrG (80 mg x kg(-1) x d(-1), via peritoneal injection), Group E of model rats treated with low dose PrG (40 mg x kg(-1) x d(-1), via peritoneal injection), and Group F of model rats treated with aspirin (25 mg x kg(-1) x d(-1), via gastrogavage), all the treatments were given in succession for 7 days. Radioimmunoassay (RIA) was used to determine serum contents of interleukin (IL)-1beta and tumor necrosis factor-alpha (TNF-alpha), immunohistochemistry was used to determine the level of COX-2 and ICAM-1 protein expression in myocardium.
RESULTSCompared with Group B, the serum level of TNF-alpha increased significantly, but not the level of IL-1beta in Group C. Besides, the COX-2 and ICAM-1 protein expressions in ischemic myocardium increased in Group C. All the above-mentioned changes were reversed to certain extent in Group E after treatment.
CONCLUSIONSPrG (40 mg x kg(-1) d(-1)) could decrease the serum level of inflammatory factor TNF-alpha, and slightly inhibit COX-2 and ICAM-1 protein expression in ischemic myocardium of AMI rats.
Animals ; Cyclooxygenase 2 ; genetics ; metabolism ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Gene Expression ; drug effects ; Humans ; Inflammation Mediators ; blood ; Intercellular Adhesion Molecule-1 ; genetics ; metabolism ; Male ; Myocardial Ischemia ; drug therapy ; genetics ; metabolism ; Myocardium ; metabolism ; Propyl Gallate ; administration & dosage ; Rats ; Rats, Wistar
9.Effect of folium panax quinquefolium saponins on apoptosis of cardiac muscle cells and apoptosis-related gene expression in rats with acute myocardial infarction.
Hui-jun YIN ; Ying ZHANG ; Yue-rong JIANG
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(3):232-235
OBJECTIVETo observe the effect of Folium Panax quinquefolium saponins (PQS) on apoptosis of cardiac muscle cells (CMCs) and apoptosis-related gene expression in rats with acute myocardial infarction (AMI).
METHODSFifty Wistar rats were randomly divided into 5 groups, 40 rats underwent coronary ligation of left anterior descending branch to establish AMI rats model, while to the other 10 rats in the sham-operation group, thoracotomy without coronary ligation was carried out. The AMI model rats in treated groups were treated with high-dosage PQS, low-dosage PQS, captopril respectively for 7 days, and those in the control group were treated with normal saline instead. Then, the apoptotic CMCs were labeled by TUNEL and the expression of Bcl-2 and Fas in cardiac muscle cells were determined by immunohistochemical methods.
RESULTSThe apoptotic index of CMCs in the model rats (46.48%) was significantly higher than that in the sham-operation group ( 1.03%, P<0.01 ). CMCs apoptosis rate in the low-dosage PQS group, the large-dosage group and the captopril group was 23.53 %, 17.58 % and 25.17 %, respectively, all were significantly lower than that in the sham-operation group (P<0.01). The expression of Bcl-2 gene was up-regulated and expression of Fas protein was down-regulated in the three treated groups.
CONCLUSIONPQS can inhibit CMCs apoptosis in AMI rats, downregulate Fas protein expression and up-regulate Bcl-2 protein expression, and has antagonistic effect in myocardial ischemic injury.
Animals ; Apoptosis ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Male ; Myocardial Infarction ; blood ; genetics ; pathology ; Myocytes, Cardiac ; pathology ; Panax ; Plant Leaves ; chemistry ; Proteins ; genetics ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; biosynthesis ; genetics ; Random Allocation ; Rats ; Rats, Wistar ; Saponins ; pharmacology
10.Efficacy of rate and rhythm control strategy in patients with atrial fibrillation: meta-analysis.
Shao-jie CHEN ; Yue-hui YIN ; Ying DONG ; Jin-qi FAN ; Li SU
Chinese Journal of Cardiology 2012;40(1):68-72
OBJECTIVEThe efficacy of rate and rhythm control strategies for treating atrial fibrillation (AF) patients was analyzed in this meta-analysis.
METHODSEligible trials were searched in MEDLINE, the Cochrane Library, the Clinical Trials, the Chinese VIP database up to May 31, 2010. Ten prospective randomized control trials with 7876 patients (rate control n = 3932, rhythm control n = 3944) were included for final analysis.
RESULTSAll cause mortality (5.3% vs. 5.0%; OR: 1.03; 95%CI: 0.84 - 1.26; I(2) < 25%) and incidence of worsening heart failure (3.81% vs. 3.61%; OR: 1.04; 95%CI: 0.80 - 1.36; I(2) < 50%) were similar between the two groups. Subgroup analysis showed that all cause mortality (3.6% vs.1.9%; OR: 1.89; 95%CI: 1.01 - 3.53; I(2) < 25%) and rate of worsening heart failure (2.3% vs. 0.3%; OR: 5.6; 95%CI: 1.44 - 21.69; I(2) < 25%) were significantly higher in rate control group than in rhythm control group in patients with age < 65 years. Thromboembolic events (1.49% vs. 1.46%; OR: 1.02, 95%CI: 0.71 - 1.48) and bleeding events (1.78% vs. 1.73%; OR: 1.02, 95%CI: 0.70 - 1.49) were similar between rhythm control and rate control groups while rehospitalization rate was significantly lower in rate control group than in rhythm control group (17.56% vs. 22.98%; OR: 0.37, 95%CI: 0.19 - 0.71).
CONCLUSIONThis meta-analysis shows that rhythm control strategy is superior to rate control strategy for AF patients with age < 65 years in terms of reducing all cause mortality and incidence of worsening heart failure.
Arrhythmias, Cardiac ; prevention & control ; Atrial Fibrillation ; physiopathology ; prevention & control ; Heart Rate ; Humans ; Prospective Studies ; Randomized Controlled Trials as Topic