1.Clinicopathological characteristics of axillary lymph node metastasis in lung cancer
Yue KONG ; Yujin XU ; Ming CHEN
Chinese Journal of Clinical Oncology 2017;44(2):87-91
Objective:To investigate the clinicopathological characteristics of lung cancer patients with axillary lymph node metastasis (ALNM). Methods:The clinical data of 91 lung cancer patients with ALNM who were treated in Zhejiang Cancer Hospital from January 1st, 2007 to December 31st, 2013 were retrospectively analyzed. The relevance of the sites of the tumor site, local lymph node, and ax-illary lymph node was checked by contingency table. Survival rates were calculated by the Kaplan-Meier method and compared by a log-rank test. Cox proportional hazards model was applied to analyze the prognostic factors. Results:The proportion of lung cancer pa-tients with ALNM was 0.63%, and the patients were often presented with adenocarcinoma, peripheral tumor type, pleura invasion with pleural effusion, or chest wall invasion. A relationship between tumor sites, local lymph node sites, and axillary lymph node sites was observed. The median survival time of lung cancer patients with ALNM was 19.02 months, and the two-year survival rate is 62.64%. Patients identified with ALNM at the initial diagnosis reported poor prognosis (P=0.002). Cox regression analysis showed that the relative risk of death in patients with ALNM at initial diagnosis was elevated 2.18 times (95%CI:1.330?3.572, P=0.003). Conclu-sion:ALNM in lung cancer is rare, and it may involve through direct chest wall invasion and spread from supraclavicular and mediasti-nal lymph node metastasis or systemic origin. Patients detected with ALNM at the initial diagnosis indicated poor prognosis.
2. Relationship of hepatitis B virus genotypes with clinicopathological characteristics in patients with chronic hepatitis B and hepatocellular carcinoma
Academic Journal of Second Military Medical University 2010;30(3):279-282
Objective: To investigate the distribution of different hepatitis B virus (HBV) genotypes in patients with chronic hepatitis B (CHB) and hepatocellular carcinoma (HCC), and to analyze the clinical laboratory examination outcomes and pathological characteristics of CHB and HCC by infection with different HBV genotypes. Methods: Totally 89 patients with CHB and 86 patients with HCC were randomly chosen for this study. HBV genotypes were determined by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) combined with double immunofluorescence staining TaqMan MGB probes. The general information and the laboratory and pathological data of patients were obtained by reviewing of the clinical documentation of patients. Statistical software SPSS10.0 was used to for statistical analyses. A P value less than 0.05 was considered statistically significant. Results: HBV B was dominant in the CHB patients in our group, accounting for 78.65%; the mixed B and C type accounted for 3.37%. HCC patients in our group were dominated by C type (70.93%). There were no other genotypes other than B and C in our group, and there was significant different between their frequency in our group (P<0.001). There were no significant differences in the clinical experimental and pathological parameters in CHB patients with different HBV subtypes. In HCC patients, those with genotype C had higher positive rate of HBV e antigen than those with genotype B(P<0.05). HCC patients infected with HBV genotype B had larger turner size (P<0.05). No associations were found between HBV genotypes with TNM stage, vascular invasion, or metastasis. Conclusion: Patients with CHB are dominantly infected with genotype B in our group. HBV genotype C and positive HBV e antigen are risk factors of HCC. Antiviral therapy and promoting e antigen seroconversion may reduce the incidence of HCC. HBV genotype B might be associated with larger tumor size.
3.Establishment of malignant pleural effusion model of Lewis lung cancer based on Micro Echo Technology
Hanze YANG ; Chunyang WANG ; Yue CHEN ; Ming JI ; Xiaoguang CHEN
Chinese Journal of Biochemical Pharmaceutics 2017;37(2):5-7
Objective To establish a malignant pleural effusion model of Lewis lung cancer in C57BL/6 mice based on Micro Echo technology. Methods Single tumor cell suspension of Lewis lung cancer was injected into thoracic cavity.The pleural effusion was detected by Micro Echo technology.The volumes of effusion were quantified and the tumor cells were counted.Results After implanted of tumor cell, malignant pleural effusion can be detected by Micro Echo technology and observed after autopsy.Chemotherapy drugs such as Cyclophosphamide and Cisplatin can decrease the effusion volumes.Conclusion Pleural effusion model of Lewis lung cancer based on Micro Echo technology can be used to evaluate the efficacy of antitumor drugs.
4.Secreted Expression of M annanase Gene in Pichia pastoris and Anylysis of Enzymic Properties
Yu QIAO ; Xiao-Bing CHEN ; Hong-Biao DING ; Ming YUE ;
China Biotechnology 2006;0(07):-
A PCR method was used to amplify the sequence encoding the mature peptide of?-mannanase of Bacillus subtilis. The gene was inserted into the Pichia pastoris vector pPIC9K, downstream of?-factor signal peptide sequence. The resultant recombinant plasmid pPIC9K-MAN was lineared by BglII digestion and introduced into the host Pichia pastoris GS115 by PEG method. After screen, the recombinant P. pastoris strain MAN22 was obtained and fermented in large scale 5L fermenter. The recombinant mannanase activity could reach to 1102IU/ml. The properties of the recombinant mannanase were characterized.
6.Clinical analysis of premedication regimen with dexamethasone at different doses for docetaxel-induced hypersensitivity reaction.
Ming-Jun ZHANG ; Zhen-Dong CHEN ; Yue-Yin PAN
Chinese Journal of Oncology 2009;31(10):795-795
Aged
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Antineoplastic Agents
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administration & dosage
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adverse effects
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therapeutic use
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Dexamethasone
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administration & dosage
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therapeutic use
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Dose-Response Relationship, Drug
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Drug Hypersensitivity
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etiology
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prevention & control
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Exanthema
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chemically induced
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prevention & control
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Fever
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chemically induced
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prevention & control
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Humans
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Middle Aged
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Neoplasms
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drug therapy
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Premedication
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Taxoids
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administration & dosage
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adverse effects
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therapeutic use
7.Application Progress in Vinblastines Combined with Traditional Chinese Medicine in the Treatment of Lung Cancer
Chen YUE ; Xianzheng WANG ; Li ZHAO ; Ming XIE
China Pharmacist 2017;20(7):1229-1232,1235
Vinblastines as third-generation chemotherapy drugs are widely used in the treatment of a variety of cancers.Among them, vinorelbine (NVB) is a first-line chemotherapy drug for non-small cell lung cancer, while its side effects often limit its clinical use.There are more and more clinical studies show that Chinese medicine and NVB in the chemotherapy program has a synergistic anti-tumor effect, which can improve quality of life and immunity of patients, and reduce such side reactions as gastrointestinal reactions, bone marrow suppression and so on.In this paper, the application progress in traditional Chinese medicine combined with vinblastine in the treatment of lung cancer in recent ten years was reviewed.
8.Carbon monoxide-releasing molecule CORM-2 protects against renal ischemia-reperfusion injury in mice
Yongle RUAN ; Lu WANG ; Yue ZHAO ; Junxiang WANG ; Song CHEN ; Changsheng MING ; Gang CHEN
Chinese Journal of Organ Transplantation 2013;34(11):685-689
Objective To investigate if the administration of CORM-2 can provide protection against renal ischemia-reperfusion injury (IRI).Method Murine renal ischemia was induced by clamping left renal pedicles for 40 min with vascular micro damps at 32 C,then the contralateral kidney was removed.CORM-2 or vehicle was administered via intravenous infusion 1 h before the onset of ischemia.The blood plasma and renal samples were obtained at 24 h after reperfusion to assess renal function and cellular injury.Plasma Cr and BUN levels,HE and TUNEL were performed to estimate the magnitude of renal damage.Kidneys were retrieved from indicated animals at various time points after renal IRI,and the sections were prepared for histological evaluation.MPO staining procedures were performed to assess the neutrophils infiltration in the renal IRI.Besides,Immunofluorescent stain of TNF-α was performed on the kidneys which were retrieved from indicated animals to determine the production of inflammatory mediators in renal I/R.Results The plasma Cr and BUN were significantly increased at 24 h after reperfusion in IRI control mice,and CORM-2 treatment could markedly diminish the increase of plasma Cr and BUN in mice subjected to I/R.In parallel,histological analysis demonstrated that CORM2 treatment markedly reduced apoptosis of the renal tubular epithelium cells and hemorrhage.IRI caused marked infiltration and accumulation of the MPO-positive neutrophils in renal interstitium.Administration of CORM-2 before ischemia dramatically inhibited neutrophils infiltration as compared with IRI or iCORM-2 group.Furthermore,we confirmed that CORM-2 markedly decreased production of TNF-α.Conclusion Carbon monoxidereleasing molecule CORM-2 could ameliorate inflammation to protect against the renal IRI in mice.
9.Alteration of tear film after sutureless large incision manual cataract extraction
Yue, ZHANG ; Wan-Rong, HUANG ; Jin-Hong, CAI ; Yan, CHEN ; Duan-Xiao, WU ; Yan Ming, HUANG
International Eye Science 2010;10(1):18-20
AIM: To investigate the alterations of tear film after sutureless large incision manual cataract extraction (SLIMCE). METHODS: Sixty-eight SLIMCE operation eyes were studied with slit-limp microscope, break- up time (BUT), SchirmmerⅠtest (SⅠt),and fluorescence(FL) to observe the alterations of tear film at different time points in postoperation. Impression cytology and microphoto-analyses technique were also applied to observe the goblet cells at different time points postoperation(7,14,30,60,90 days). RESULTS: Subjective complaint of dry eye within 90 days after the operations were significantly increased compare with preoperations(5-27,23,19,16,13; 2-16,14,8,6,3). The schirmmer Ⅰ test were greatly increased in 14 days postoperation(10.1±4.5;15.0±4.7,13.8±5.7),the mean scores of fluorescence increased (0-17,9,5;0-8,3,1) and the mean break-up time decreased in 30 days post-operation(10.3±2.2;5.5±2.3,7.0±2.4,7.9±2.2) (P<0.05). CONCLUSION: SLIMCE operation have effect on the stability of tear film.
10.Size-dependent biological effects on vascular endothelial cells induced by different particulate matters.
Wen-Juan, CHENG ; Yi, RONG ; Ting-Ming, SHI ; Ting, ZHOU ; Yue-Wei, LIU ; Wei-Hong, CHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(3):314-21
The contribution of particles to cardiovascular mortality and morbidity has been enlightened by epidemiologic and experimental studies. However, adverse biological effects of the particles with different sizes on cardiovascular cells have not been well recognized. In this study, sub-cultured human umbilical vein endothelial cells (HUVECs) were exposed to increasing concentrations of pure quartz particles (DQ) of three sizes (DQPM1, <1 μm; DQPM3-5, 3-5 μm; DQPM5, 5 μm) and carbon black particles of two sizes (CB0.1, <0.1 μm; CB1, <1 μm) for 24 h. Cytotoxicity was estimated by measuring the activity of lactate dehydrogenase (LDH) and cell viability. Nitric oxide (NO) generation and cytokines (TNF-α and IL-1β) releases were analyzed by using NO assay and enzyme-linked immunoabsorbent assay (ELISA), respectively. It was found that both particles induced adverse biological effects on HUVECs in a dose-dependent manner. The size of particle directly influenced the biological activity. For quartz, the smaller particles induced stronger cytotoxicity and higher levels of cytokine responses than those particles of big size. For carbon black particles, CB0.1 was more capable of inducing adverse responses on HUVECs than CB1 only at lower particle concentrations, in contrast to those at higher concentrations. Meanwhile, our data also revealed that quartz particles performed stronger cell damage and produced higher levels of TNF-α than carbon black particles, even if particles size was similar. In conclusion, particle size as well as particle composition should be both considered in assessing vascular endothelial cells injury and inflammation responses induced by particles.