1.PROGRESS IN RESEARCH OF MICROORGANISMS OF NATURAL ENVIRONMENTS IN THE VIABLE BUT NON-CULTURABLE STATE
Xiu-Juan YUE ; Li-Yan YU ; Yue-Qin ZHANG ;
Microbiology 1992;0(02):-
At the beginning of the 1980s, a concept of viable but non-culturable(VBNC) was suggested. VBNC is a survival strategy adopted by microorganisms when they are exposed to environmental stress. This article try to make a summary of research of the conditions of VBNC formation, recovery of culturability and methods of VBNC cells detection. In addition, introduces the first growth factor of microorganisms-Rpf.
2.Study of Methods to Isolate Viable but Non-culturable Microorganisms from Natural Environments
Xiu-Juan YUE ; Li-Yan YU ; Qiu-Ping LI ; Yu-Zhen WEI ; Yan GUAN ; Yue-Qin ZHANG ;
Microbiology 1992;0(03):-
This project is targeted on exploring some improving approaches to isolate and culture the microorganisms which are difficult to be isolated and cultured through the conventional ways. The results showed that betaine, sodium pyruvate, SOD and catalase are helpful for increasing the total number and variety of isolated strains. A kind of combined method was also used to isolate the micro-colony which can not be seen by naked eyes on the plates. Totally 52 Actinomycetes and 103 bacteria and 17 fungi were obtained from 4 soil samples using the above methods. 4. 325% microorganisms were obtained as positive strains to inhibit the growth of some kinds of test bacteria, which is higher than the percent using generally isolated ones. These microbial natural products may remain an important resource for the drug discovery.
3.Effect of comprehensive intervention on antimicrobial prophylaxis in clean incision surgery
Yinghua ZHANG ; Jinai QIN ; Lanlan LI ; Juan HUANG ; Xiaohong HUANG ; Jingjing YA ; Qunxiu GUO ; Yue QIU ; Xibing WANG ; Daohai CHENG
Chinese Journal of Infection Control 2016;15(2):111-113
Objective To evaluate the effect of comprehensive intervention on perioperative antimicrobial prophy-laxis in clean incision surgery in a hospital.Methods From 2011 ,clean incision surgery cases were performed com-prehensive intervention,antimicrobial use in 2011 -2013 were compared.Results A total of 5 945 cases of clean in-cision surgeries were investigated between 2011 and 2013,3 827 cases (64.37%)received prophylactic use of anti-microbial agents.Prophylactic antimicrobial usage rates in 2011 -2013 were 84.95%,69.99%,and 52.97% re-spectively(χ2 =380.94,P <0.001);the correct rates of medication time were 50.97%,79.99%,and 98.95% re-spectively(χ2 =827.02,P <0.001 );the percentages of prophylactic antimicrobial use ≤24 hours were 24.91 %, 39.96%,and 64.95% respectively(χ2 =422.55,P <0.001 );additional antimicrobial usage rates during surgery were 50.00%,60.00%,and 80.00% respectively(χ2 =59.47,P <0.001 ).Conclusion The implementation of comprehensive intervention measures can standardize antimicrobial use,reduce prophylactic antimicrobial usage rate,improve the correct rate of medication time,shorten the duration of antimicrobial use,and implement addition-al use of antimicrobial agents during surgery.
4.Nursing of the morbidly obese patients treated with laparoscopic vertical banded gastroplasty
Yue-Feng WU ; Ping CAO ; Xiao-Li GE ; Chun-Juan ZHOU ; Lian-Qin XU
Academic Journal of Second Military Medical University 2001;22(5):478-480
Objective: To study the nursing of patients with morbid obesity treated with laparoscopic vertical banded gastroplasty (LVBG). Methods: Before operation, obese degree, obesity-related conditions and mental states were examined routinely. Monitoring of respiratory tract, observing operative complications and instructing of diets were done after operation. Results: Among 6 patients, 5 were at the third degree of obese, one was at second. In obesity-related conditions, 4 patients had hypertension and acantha derma, 1 had arthritis, and all had respiratory sleeping syndrome. The operations were all successful. The food amount food and body weight both decreased significantly 1 month after operation. The common operative complications were mild bleeding (1 case), shoulder-back pain (1 case), nausea and vomiting (5 cases). Diet principle was high protein, low energy, liquid food was the first choice. Conclusion: Observing and preventing respiratory sleeping syndrome are the main points of postoperative cares. Instructing patients to establish correct diet habit is the key to reach the best efficacy of LVBG.
5.Relationship between serum HBV DNA level and follicular helper T lymphocyte in patients with chronic hepatitis B and its significance.
Juan-Hua WANG ; Xi-Bing GU ; Yin-Fang ZHU ; Zhong HUA ; Dong WANG ; Xiao-Juan YANG ; Yue-Qin XU ; Zhong-Hua LU
Chinese Journal of Experimental and Clinical Virology 2013;27(5):351-353
OBJECTIVETo explore relationship between HBV DNA level and peripheral blood follicular helper T lymphocyte (Tfh) in patients with chronic hepatitis B (CHB) and its significance.
METHODSHBV DNA levels of 179 cases of CHB patients with positive HBV DNA, positive HBeAg and positive human leukocyte antigen(HLA)-A2 were tested with real time fluorescent quantitative PCR. Tfh and HBV specific CTL were tested with flow cytometry. IL-21 was also tested. 179 cases of CHB patients were divided into group A and group B based on HBV DNA levels, 86 cases in group A, HBV DNA levels were 10(4)-10(5) copies/ml, 93 cases in group B, HBV DNA levels were 10(6)-10(7) copies/ml. Above testing indexes of the two groups were compared.
RESULTSHBV DNA levels of group A were (4.85 +/- 0.37) log10 copies/ml, HBV DNA levels of group B were (6.83 +/- 0.31 ) log10 copies/ml, t = 27.31, P < 0. 001; Tfh of group A was (5.96 +/- 1.59)%, higher than that of group B (3.71 +/- 2.15)%, t = 4.92, P < 0.01; IL-21 of group A was (42.61 +/- 15.11)ng/L, higher than that of group B (14.91 +/- 3.15) ng/L, t = 8.62, P < 0.01; HBV specific CTL of group A was (0.36 +/- 0.08)%, higher than that of group B (0.18 +/- 0.06)%, t = 19.99, P < 0.001.
CONCLUSIONSerum HBV DNA level of CHB patients is related to the level of peripheral blood Tfh level: patients with low HBV DNA level have high Tfh level, high IL-21 level and high HBV specific CTL level. Patients with high HBV DNA level have low Tfh level, low IL-21 level and low HBV specific CTL level. The mechanism of baseline HBV DNA level affecting anti-viral therapy may be related to Tfh level.
Adult ; CD4 Lymphocyte Count ; DNA, Viral ; blood ; genetics ; Female ; HLA-A2 Antigen ; immunology ; Hepatitis B virus ; genetics ; isolation & purification ; Hepatitis B, Chronic ; blood ; immunology ; virology ; Humans ; Interleukins ; immunology ; Male ; T-Lymphocytes, Helper-Inducer ; cytology
6.Unusual prostate carcinoma characterized by extensive metastasis, significantly increased serum level of prostatic-specific antigen,and neuroendocrine differentiation: a case report.
Yu-Xin HU ; Juan YE ; Ying JIANG ; Qin-Fang ZHANG ; Yue-Long WU ; Yue-Yu CHEN
Chinese Medical Journal 2005;118(3):258-261
7.Association between mother's periodontal status and preterm low birth weight in Beijing.
Jun KANG ; Yue-qin SHA ; Lu HE ; Zhi-bin CHEN ; Jin-juan LIANG ; Zhen HUANG
Chinese Journal of Stomatology 2009;44(10):580-583
OBJECTIVETo investigate the possible association between mother's periodontal status and preterm low birth weight (PLBW) in Beijing.
METHODSThe periodontal status of 83 women who bore a preterm low birth weight infant (PLBW group) and 44 women who bore a normal birth weight infant (NBW group) within two years in four hospitals in Beijing were evaluated. These women were all generally healthy. The clinical periodontal parameters including plaque index (PLI), probing depth (PD), attachment loss (AL) and bleeding index (BI) recorded.
RESULTSThe percentages of PD > or = 4 mm sites, AL > or = 2 mm sites and BOP(+) sites in two groups were 8.1% vs 6.9%, 12.8% vs 6.1% and 70.4% vs 66.9%, respectively, which showed significantly higher in PLBW group than in NBW group(P < 0.01, P < 0.001, P < 0.01).
CONCLUSIONSThere was possible association between mother's periodontal status and preterm low birth weight in the investigated area. Poor periodontal status may be one of the risk factors of PLBW.
China ; epidemiology ; Female ; Humans ; Infant, Low Birth Weight ; Mothers ; Periodontal Diseases ; epidemiology ; Premature Birth ; etiology ; Risk Factors
8.The effects of demographic features on differences in sensitivity between PCL-C and SCL-90 scores in a follow-up study in secondary school students in the Wenchuan earthquake region.
Feng Su HOU ; Ting LI ; Juan LI ; Xiao Qin HU ; Zhi Yue LIU ; Ping YUAN
Biomedical and Environmental Sciences 2011;24(6):642-648
OBJECTIVETo analyze the sensitivity of effect factors between the PCL-C and the SCL-90, to provide evidence for social psychological crisis screening and post-trauma interventions.
METHODSWe administered the PCL-C and SCL-90 to screen for PTSD and other psychological problems among students who survived the disaster and continued their school studies. The surveys were carried out 3, 6, 9, and 12 months after the earthquake. A bivariate 2-level logistic model was used to explore the different levels of sensitivity among students. The factors influencing the relationships between PTSD and depression, and between PTSD and anxiety were examined.
RESULTSWe analyzed data from 1 677 students, revealing that female students in higher grades were more likely to exhibit symptoms of depression, rather than PTSD, compared with the control group (males in lower grades), and the difference was significant (P<0.05). In contrast, ethnic minorities were more likely to exhibit PTSD symptoms compared to the others. In addition, female students were more likely to exhibit symptoms of anxiety than PTSD. Other effects that did not reach statistical significance were suggested to have a similar influence on PTSD, depression, and anxiety.
CONCLUSIONAfter a natural disaster, specific aspects of depression and anxiety should be examined, avoiding an overemphasis on PTSD in social psychological crisis interventions.
Adolescent ; Age Factors ; Child ; China ; Depression ; etiology ; psychology ; Disasters ; Earthquakes ; Female ; Humans ; Logistic Models ; Male ; Psychiatric Status Rating Scales ; Sex Factors ; Stress Disorders, Post-Traumatic ; etiology ; psychology ; Students ; psychology ; Surveys and Questionnaires ; Young Adult
9.A preliminary study on serum protein SP70 as a novel biomarker for the detection of non-small cell lung cancer
Ying PENG ; Shiyang PAN ; Fang WANG ; Peijun HUANG ; Ting XU ; Lei HUANG ; Lixia ZHANG ; Qing LI ; Hongmei ZHANG ; Jian XU ; Xuejun QIN ; Yue HAN ; Juan XU ; Shanjun ZHU
Chinese Journal of Laboratory Medicine 2012;35(6):554-558
Objective To evaluate whether the protein SP70 could be used as a serum biomarker for the diagnosis of non-small cell lung cancer (NSCLC).Methods Polyclonal antibody was prepared by immunizing New Zealand rabbit with SPC-A1 cells.Sandwich ELISA was carried out by using newly-prepared polyclonal antibody(PcMb) coating assay plates,monoclonal antibody (McAb) NJ001 and HRP goat antimouse antibody as primary antibody and labeling antibody respectively.After optimizing the experiment conditions,serum from 175 lung cancer patients [ 80 NSCLC adenocarcinoma,70 NSCLC squamous carcinoma and 25 small cell lung cancer ( SCLC) ],25 benign lung disease ( BLD) patients and 300 healthy controls (HC) were examined.CEA,NSE,CYFRA21-1 were measured by ECLIA for comparison.Results Positive rates of NSCLC adenocarcinoma,NSCLC squamous carcinoma,SCLC and BLD were 68.8%,51.4%,16.0% and 12.0% respectively,obviously higher than that of HC (7.3%).NSCLC (adenocarcinoma,Squamous carcinoma) had significantly higher positive rate than SCLC (60.7% υs 16.0%,x2 =17.23,P<0.05)and BLD(60.7% υs 12.0%,x2 =20.41,P <0.05).Among 68 NSCLC patients who had definite staging,positive rates at early stage ( Ⅰ/Ⅱ,n=30) reached up to 76.7%.Meanwhile,positive rates of CEA,NSE and CYFRA21-1 (32.7%,18.0% and 37.3%) were significantly lower than the targeting antigen to McAb NJ001 in NSCLC(60.7% υs 32.7%,x2 =23.63,P <0.05;60.7%υs18.0%,x2 =57.22,P<0.05;60.7% υs37.3%,x2=16.34,P<0.05).Conclusions It showed high positive rates of SP70 in the serum of NSCLC patients,which suggested thai SP70 might be a potential valuable biomarker for the diagnosis of NSCLC.
10.Relations between ALT level and count of HBV special CTL and non-specific CTL in patients with chronic hepatitis B.
Xi-bin GU ; Xiao-juan YANG ; Dong WANG ; Zhong HUA ; Hang-yuan WU ; Yue-qin XU ; Zhong-hua LU
Chinese Journal of Experimental and Clinical Virology 2009;23(5):343-345
OBJECTIVETo explore relations between ALT level and hepatitis B virus (HBV) specific CTL and non-specific CTL in patients with chronic hepatitis B (CHB).
METHODS148 cases of CHB were divided into three groups according to ALT level. 35 cases in group A, ALT > or =2 x upper limit of normal value (ULN)--5 x ULN (100-250 IU/L); 53 cases in group B, ALT > 5 x ULN-- < or =10 x ULN (251-500 IU/L); 60 cases in group C, ALT > 10 x ULN ( > 500 IU/L). Flow cytometry is used to determine non-specific CTV. HBV specific CTL was tested on 74 cases of CHB (17 in group A, 27 in group B and 30 in group C) with positive (HLA)-A2. Compare HBV specific CTL, non-specific CTL, HBV DNA levels and positive rate of HBeAg.
RESULTSHBV specific CTL: Group A (0.42 +/- 0.10)% is higher than group B (0.25 +/- 0.08)%, t = 6.37, P < 0.01, group B is higher than group C (0.17 +/- 0.004)%, t = 5.14, P < 0.01; Non-specific CTL: Group A (15.01 +/- 3.01)% is lower than group B (18.1 +/- 5.02)%, t = 2.81, P < 0.01, group B is lower than group C (21.5 +/- 6.11)%, t = 3.07, P < 0.01; HBV DNA level: Group A [(4.97 +/- 0.86) log10 copies/ml] is lower than group B [(5.92 +/- 0.92) log10 copies/ml], t = 4.87, P < 0.01. Group B is lower than group C [(6.37 +/- 0.71) log10 copies/ml], t = 2.92, P < 0.01; Positive HBeAg: Group A (15 cases, 42.86%) is lower than group B (32 cases, 60.38%), chi2 = 2.59, P > 0.05. Group B is lower than group C (41 cases, 68.33%), chi2 = 0.78, P > 0.05. Group A is lower than group C, chi2 = 5.929, P < 0.05.
CONCLUSIONThe higher the non-specific CTL of patients with CHB is, the higher the ALT level would be, whereas the lower the HBV specific CTL is, the stronger the HBV replication would be.
Adult ; Alanine Transaminase ; metabolism ; Female ; Hepatitis B virus ; genetics ; immunology ; physiology ; Hepatitis B, Chronic ; enzymology ; immunology ; virology ; Humans ; Lymphocyte Count ; Male ; T-Lymphocytes, Cytotoxic ; immunology ; Virus Replication ; Young Adult